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- Loring, Zak, et al.
(författare)
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Acute echocardiographic and hemodynamic response to his-bundle pacing in patients with first-degree atrioventricular block
- 2022
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Ingår i: Annals of Noninvasive Electrocardiology. - : Wiley. - 1082-720X .- 1542-474X. ; 27:4
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Tidskriftsartikel (refereegranskat)abstract
- Background: Atrial pacing and right ventricular (RV) pacing are both associated with adverse outcomes among patients with first-degree atrioventricular block (1°AVB). His-bundle pacing (HBP) provides physiological activation of the ventricle and may be able to improve both atrioventricular (AV) and inter-ventricular synchrony in 1°AVB patients. This study evaluates the acute echocardiographic and hemodynamic effects of atrial, atrial-His-bundle sequential (AH), and atrial-ventricular (AV) sequential pacing in 1°AVB patients. Methods: Patients with 1°AVB undergoing atrial fibrillation ablation were included. Following left atrial (LA) catheterization, patients underwent atrial, AH- and AV-sequential pacing. LA/left ventricular (LV) pressure and echocardiographic measurements during the pacing protocols were compared. Results: Thirteen patients with 1°AVB (mean PR 221 ± 26 ms) were included. The PR interval was prolonged with atrial pacing compared to baseline (275 ± 73 ms, p =.005). LV ejection fraction (LVEF) was highest during atrial pacing (62 ± 11%), intermediate with AH-sequential pacing (59 ± 7%), and lowest with AV-sequential pacing (57 ± 12%) though these differences were not statistically significant. No significant differences were found in LA or LV mean pressures or LV dP/dT. LA and LV volumes, isovolumetric times, electromechanical delays, and global longitudinal strains were similar across pacing protocols. Conclusion: Despite pronounced PR prolongation, the acute effects of atrial pacing were not significantly different than AH- or AV-sequential pacing. Normalizing atrioventricular and/or inter-ventricular dyssynchrony did not result in acute improvements in cardiac output or loading conditions.
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| 2. |
- Bergentoft, Helene, 1964, et al.
(författare)
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Teachers' actor-oriented transfer of movement pedagogy knowledge in physical education
- 2022
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Ingår i: Physical Education and Sport Pedagogy. - : Routledge. - 1740-8989 .- 1742-5786.
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Tidskriftsartikel (refereegranskat)abstract
- Background: Physical education (PE) teachers in practically all countries are expected to help their students develop movement capability. To achieve this objective, teachers need certain knowledge and competencies. The question of how PE teachers should develop their capacities to achieve this task has received only limited research attention.Aim: The broad objective of this paper is to contribute to the literature on how PE teachers can develop knowledge and competencies in the area of movement capability related to students' learning. The specific aim is to identify aspects of the design of instruction in physical education that enhance teachers' actor-oriented transfer of movement pedagogy knowledge, during a collaborative professional development intervention.Method: The study is an analysis of three conducted learning studies in PE at upper secondary schools in Sweden. The studies involved seven PE teachers from two different schools. Our empirical material consists of (a) notes from team meetings (n = 14), (b) lesson plans (n = 9), (c) video-recorded and transcribed lessons (n = 9), and (d) results of students' learning outcomes (n = 9).Findings: PE teachers' analysis of their own teaching sequences in teams supported their actor-oriented transfer of movement pedagogy knowledge, which developed their abilities to further elaborate their instruction in new teaching situations. Moreover, teachers gained insights into how to further develop the quality of instructional design as expansions of earlier experiences. Lastly, a relationship between PE teachers' actor-oriented transfer and students' increased learning of movements was found.Conclusion: Our conclusion is that collaborative professional development for PE teachers, which supports actor-oriented transfer, should be offered to enhance teachers' movement pedagogy knowledge.
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| 5. |
- Habbouche, S., et al.
(författare)
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Comparison of the novel WEst coast System for Triage (WEST) with Rapid Emergency Triage and Treatment System (RETTS (c)): an observational pilot study
- 2022
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Ingår i: International Journal of Emergency Medicine. - : Springer Science and Business Media LLC. - 1865-1372 .- 1865-1380. ; 15:1
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Tidskriftsartikel (refereegranskat)abstract
- Background Most Swedish emergency departments (ED) use the triage system Rapid Emergency Triage and Treatment System (RETTS (c)), which over time has proven to prioritize patients to higher triage levels. When many patients are prioritized to high triage levels, challenges with identifying true high-risk patients and increased waiting time for these patients has emerged. In order to achieve a more balanced triage in relation to actual medical risk, the triage system WEst coast System for Triage (WEST) was developed, based on the South African Triage Scale (SATS). The aim of this study was to perform an initial evaluation of the novel emergency triage system WEST compared to the existing RETTS (c). Methods Both RETTS (c) and WEST are five level triage systems illustrated by colors. Nurses from each of the three adult EDs of Sahlgrenska University Hospital in Gothenburg and the ambulance service assessed and triaged 1510 patients according to RETTS (c) and immediately thereafter filled out the WEST triage form. Data from each triage report were analyzed and grouped according to the triage color, chief complaint, and outcome of each patient. Data on discharge categories and events within 72 h were also collected. Data were analyzed with descriptive statistical methods. Results In general, WEST displayed lower levels of prioritization compared to RETTS (c), with no observed impact on patients' medical outcomes. In RETTS (c) orange triage level, approximately 50% of the patients were down prioritized in WEST to yellow or green triage levels. Also, in the RETTS (c) yellow triage level, more than 55% were down prioritized to green triage level in WEST. The number of patients who experienced a serious event during the first 72 h was few. Three patients died, these were all prioritized to red triage level in RETTS (c). In WEST two of these patients were prioritized to red triage level and one to orange triage level. All these patients were admitted to hospital before deterioration. Conclusions WEST may reduce over prioritization at the ED, especially in the orange and yellow triage levels of RETTS (c), with no observed increase in medical risk. WEST can be recommended for a clinical comparative study.
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| 6. |
- Holmqvist, Anna Sällfors, et al.
(författare)
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Severe, life-threatening, and fatal chronic health conditions after allogeneic blood or marrow transplantation in childhood
- 2023
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Ingår i: Cancer. - : Wiley. - 0008-543X .- 1097-0142. ; 129:4, s. 624-633
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Tidskriftsartikel (refereegranskat)abstract
- Background: A comprehensive assessment of morbidity after allogeneic bone marrow transplantation (BMT) performed in childhood remains understudied. Methods: Seven hundred eighty-nine allogeneic BMT recipients who had survived ≥2 years after BMT performed between 1974 and 2014 at age <22 years and 690 siblings completed a 255-item survey self-reporting sociodemographics and chronic health conditions. A severity score (grade 3 [severe], 4 [life-threatening], or 5 [fatal]) was assigned to the conditions using Common Terminology Criteria for Adverse Events, version 5.0. For the BMT cohort, the cumulative incidence of chronic health conditions was calculated as a function of time from BMT. Proportional subdistribution hazards models were used to determine predictors of grade 3–5 conditions. Logistic regression was used to estimate the risk of grade 3–4 conditions in BMT recipients who were alive at the time of this study compared with siblings. Results: The median age at transplantation was 11.3 years (range, 0.4–22.0 years), and the median length of follow-up was 11.7 years (range, 2.0–45.3 years). The most prevalent primary diagnoses were acute lymphoblastic leukemia (30.7%), and acute myeloid leukemia/myelodysplastic syndrome (26.9%). At age 35 years, the cumulative incidence of a grade 3–4 condition was 53.8% (95% CI, 46.7%–60.3%). The adjusted odds ratio of a grade 3–4 condition was 15.1 in survivors (95% CI, 9.5–24.0) compared with siblings. The risk of a grade 3–5 condition increased with age at BMT (hazard ratio [HR], 1.03; 95% CI, 1.01–1.05) and was higher among females (HR, 1.27; 95% CI, 1.02–1.59), patients who received total body irradiation (HR, 1.71; 95% CI, 1.27–2.31), and those reporting chronic graft-versus-host disease (HR, 1.38; 95% CI, 1.09–1.74). Conclusions: Two-year survivors of allogeneic BMT in childhood have an increased risk of grade 3–4 chronic health conditions compared with siblings, suggesting the need for long-term follow-up.
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| 8. |
- Johansson, S, et al.
(författare)
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Participation in social/lifestyle activities in people with multiple sclerosis: Changes across 10 years and predictors of sustained participation
- 2020
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Ingår i: Multiple sclerosis (Houndmills, Basingstoke, England). - : SAGE Publications. - 1477-0970 .- 1352-4585. ; 26:13, s. 1775-1784
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Tidskriftsartikel (refereegranskat)abstract
- Identification of people with multiple sclerosis (PwMS) with increased risk of restricted participation in social and lifestyle activities (e.g. social outings and pursuing a hobby) could guide the development of interventions supporting sustained participation. Objective: To explore changes in participation in complex and social everyday activities over 10 years in PwMS in relation to multiple sclerosis (MS) severity and to identify predictors of sustained participation. Methods: This study was based on a 10-year follow-up of 264 PwMS living in Stockholm County, Sweden. Ten-year changes in participation in social/lifestyle activities were assessed and compared between PwMS with different MS severity with the Frenchay Activities Index using age- and sex-related normative values. Multiple logistic regression analyses were used to predict sustained participation at 10 years using personal factors, disease severity and functioning as independent variables. Results: While a majority of people with mild MS demonstrated sustained participation (67%), a minority of PwMS moderately (26%) and severely affected by MS (5%) demonstrated sustained participation. Significant predictors of sustained participation after 10 years were walking speed ⩾1.2 m/s and ⩾32 correct responses on the Symbol Digit Modalities Test. Conclusion: Our findings accentuate the importance for health services to support mobility and cognition to obtain sustained participation.
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| 9. |
- Redlinger-Pohn, Jakob D., et al.
(författare)
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Mechanisms of Cellulose Fiber Comminution to Nanocellulose by Hyper Inertia Flows
- 2022
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Ingår i: ACS Sustainable Chemistry and Engineering. - : American Chemical Society. - 2168-0485. ; 10:2, s. 703-719
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Tidskriftsartikel (refereegranskat)abstract
- Nanocelluloses are seen as the basis of high-performance materials from renewable sources, enabling a bio-based sustainable future. Unsurprisingly, research has initially been focused on the design of new material concepts and less on new and adapted fabrication processes that would allow large-scale industrial production and widespread societal impact. In fact, even the processing routes for making nanocelluloses and the understanding on how the mechanical action fibrillates plant raw materials, albeit chemically or enzymatically pre-treated, are only rudimentary and have not evolved significantly during the past three decades. To address the challenge of designing cellulose comminution processes for a reliable and predictable production of nanocelluloses, we engineered a study setup, referred to as Hyper Inertia Microfluidizer, to observe and quantify phenomena at high speeds and acceleration into microchannels, which is the underlying flow in homogenization. We study two different channel geometries, one with acceleration into a straight channel and one with acceleration into a 90° bend, which resembles the commercial equipment for microfluidization. With the purpose of intensification of the nanocellulose production process, we focused on an efficient first pass fragmentation. Fibers are strained by the extensional flow upon acceleration into the microchannels, leading to buckling deformation and, at a higher velocity, fragmentation. The treatment induces sites of structural damage along and at the end of the fiber, which become a source for nanocellulose. Irrespectively on the treatment channel, these nanocelluloses are fibril-agglomerates, which are further reduced to smaller sizes. In a theoretical analysis, we identify fibril delamination as failure mode from bending by turbulent fluctuations in the flow as a comminution mechanism at the nanocellulose scale. Thus, we argue that intensification of the fibrillation can be achieved by an initial efficient fragmentation of the cellulose in smaller fragments, leading to a larger number of damaged sites for the nanocellulose production. Refinement of these nanocelluloses to fibrils is then achieved by an increase in critical bending events, i.e., decreasing the turbulent length scale and increasing the residence time of fibrils in the turbulent flow. © 2022 The Authors.
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| 10. |
- Stenum, Thomas Søndergaard, et al.
(författare)
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RNA interactome capture in Escherichia coli globally identifies RNA-binding proteins
- 2023
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Ingår i: Nucleic Acids Research. - : Oxford University Press. - 0305-1048 .- 1362-4962. ; 51:9, s. 4572-4587
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Tidskriftsartikel (refereegranskat)abstract
- RNA-binding proteins (RPBs) are deeply involved in fundamental cellular processes in bacteria and are vital for their survival. Despite this, few studies have so far been dedicated to direct and global identification of bacterial RBPs. We have adapted the RNA interactome capture (RIC) technique, originally developed for eukaryotic systems, to globally identify RBPs in bacteria. RIC takes advantage of the base pairing potential of poly(A) tails to pull-down RNA-protein complexes. Overexpressing poly(A) polymerase I in Escherichia coli drastically increased transcriptome-wide RNA polyadenylation, enabling pull-down of crosslinked RNA-protein complexes using immobilized oligo(dT) as bait. With this approach, we identified 169 putative RBPs, roughly half of which are already annotated as RNA-binding. We experimentally verified the RNA-binding ability of a number of uncharacterized RBPs, including YhgF, which is exceptionally well conserved not only in bacteria, but also in archaea and eukaryotes. We identified YhgF RNA targets in vivo using CLIP-seq, verified specific binding in vitro, and reveal a putative role for YhgF in regulation of gene expression. Our findings present a simple and robust strategy for RBP identification in bacteria, provide a resource of new bacterial RBPs, and lay the foundation for further studies of the highly conserved RBP YhgF.
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