| 1. |
- Holgersson, J, et al.
(författare)
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Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.
- 1990
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Ingår i: Glycoconjugate journal. - 0282-0080. ; 7:6, s. 601-8
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Tidskriftsartikel (refereegranskat)abstract
- Total non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region.
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| 2. |
- Holgersson, J, et al.
(författare)
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Blood group A glycolipid antigen biosynthesis: discrimination between biosynthesized and enzyme preparation derived blood group A antigen by mass spectrometry.
- 1990
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Ingår i: Analytical biochemistry. - 0003-2697. ; 184:1, s. 145-50
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Tidskriftsartikel (refereegranskat)abstract
- A monofucosyl type 1 chain blood group A hexaglycosylceramide was biosynthesized in solution using the type 1 chain blood group H pentaglycosylceramide as precursor, a crude microsomal fraction prepared from the mucosa scraping of a blood group A pig small intestine as enzyme source, and uridine diphosphate-N-acetyl-(1-14C)galactosamine as sugar donor. The radioactive product was enriched using reversed-phase column chromatography and silica gel HPLC. The peak, as detected by a beta-flow scintillation counter, was collected, permethylated, and analyzed by mass spectrometry. Carbohydrate sequence ions were found, indicating the presence of both the biosynthesized and a native, non-14C-containing blood group A hexaglycosylceramide. The blood group A pig small intestinal mucosa used as the enzyme source contain blood group A hexaglycosylceramide as the predominant glycolipid. Therefore, it is concluded that the nonradioactive blood group A hexaglycosylceramide found after the biosynthesis is derived from the enzyme preparation.
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| 3. |
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| 4. |
- Jacobsson, Per, et al.
(författare)
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Reorientational motion of the NO3− ion through the liquid-glass transition in Ca0.4K0.6(NO3)1.4 and Ca(NO3)2 + 8H2O
- 1994
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Ingår i: Journal of Non-Crystalline Solids. - : Elsevier. - 0022-3093 .- 1873-4812. ; 172-174:Part 1, s. 161-166
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Tidskriftsartikel (refereegranskat)abstract
- The reorientational correlation function of the NO3- ion in Ca0.4K0.6(NO3)1.4 and in Ca(NO3)2 + 8H2O has been investigated by Raman spectroscopy from the low viscosity liquid at high temperatures through the liquid-glass transformation region and into the frozen glassy state. It was obtained from the reorientational contribution to the 1050 cm-1 symmetric stretch of the NO3- ion, determined from a comparison of the anisotropic and isotropic parts of the Raman band. It was found that the NO3- ion displays rapid reorientational motion over the entire temperature range studied, i.e., also well below the glass transition temperature, Tg. Two types of rotational motion were revealed from the time evolution of the reorientational correlation function which shows a change of behaviour around 0.2 ps. The fast process was attributed to a free-rotor-type motion and the slower process to reorientational diffusion. The relaxation time of the latter, τor, was observed to follow an Arrhenius temperature dependence over the range from above the melting temperature to well below Tg. It was found that τor decouples from the structural relaxation time at temperatures below ∼1.7Tg in the two systems studied.
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| 5. |
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| 6. |
- Wasserman, D, et al.
(författare)
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Parasuicide in the Nordic countries.
- 1994
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Ingår i: Scandinavian journal of social medicine. - 0300-8037. ; 22:3, s. 170-7
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Tidskriftsartikel (refereegranskat)abstract
- This article gives an account of some findings of the WHO/EURO multicentre study on parasuicide from the five Nordic centres: Helsinki (Finland), Stockholm (Sweden), and the countries of Funen (Denmark), Sø-Trøndelag (Norway) and Västerbotten (Sweden). For parasuicide patients treated in hospital in these five Nordic centres, the parasuicide rates per 100,000 inhabitants per annum were 222 for women, 213 for men and 224 for both sexes combined. In all the Nordic centres except Helsinki, women had higher parasuicide rates than men. The female/male parasuicide ratio was 1.16 for all Nordic centres. The highest parasuicide rates in the whole material were found in Helsinki, Stockholm and Funen, and the lowest in Sør-Trøndelag and Västerbotten. The highest parasuicide rates lay in the 30-39 age interval, i.e. the peak of parasuicide rates was in older age groups than previously reported. Single people, especially divorcees and those who have never been married, constitute a high-risk group in both sexes. The risk of parasuicide for single men is three times that for married men in all the Nordic centres except Sør-Trøndelag, where the risk is five times higher. The parasuicide risk for single women is twice as high as for married women except for women in Funen, where the risk for single and married women is almost equal. Exceptionally high parasuicide rates are also reported for Finnish citizens in Sweden, compared with Finns from the Helsinki centre. Parasuicide rates appear to be correlated with population density, since the densely populated areas of Helsinki, Stockholm and Funen evince higher parasuicide rates than the sparsely populated areas of Sør-Trøndelag and Västerbotten.
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| 7. |
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