| 1. |
- Adams, Robin, et al.
(författare)
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What is the word for 'Engineering' in Swedish : Swedish students conceptions of their discipline
- 2007
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Engineering education in Sweden – as in the rest of the world – is experiencing a decline in student interest. There are concerns about the ways in which students think about engineering education, why they join an academic programme in engineering, and why they persist in their studies. In this context the aims of the Nationellt ämnesdidaktiskt Centrum för Teknikutbildning i Studenternas Sammanhang project (CeTUSS) is to investigate the student experience and to identify and support a continuing network of interested researchers, as well as in building capacity for disciplinary pedagogic investigation. The Stepping Stones project brings together these interests in a multi-researcher, multi-institutional study that investigates how tudents and academic staff perceive engineering in Sweden and in Swedish education. The first results of that project are reported here. As this study is situated uniquely in Swedish education, it allows for exploration of “a Swedish perspective” on conceptions of engineering. The Stepping Stones project was based on a model of research capacity-building previously instantiated in the USA and Australia (Fincher & Tenenberg, 2006).
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| 2. |
- Björk, Jonas, et al.
(författare)
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Skydda naturen nära oss
- 2007
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Ingår i: Skånska dagbladet. - 1103-9973. ; , s. 5-5
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 3. |
- Björklund, Elisabet, et al.
(författare)
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Quality control of flow cytometry data analysis for evaluation of minimal residual disease in bone marrow from acute leukemia patients during treatment.
- 2009
-
Ingår i: Journal of pediatric hematology/oncology : official journal of the American Society of Pediatric Hematology/Oncology. - : Lippincott Williams & Wilkins. - 1536-3678 .- 1077-4114. ; 31:6, s. 406-15
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Tidskriftsartikel (refereegranskat)abstract
- Low levels of leukemia cells in the bone marrow, minimal residual disease (MRD), are considered to be a powerful indicator of treatment response in acute lymphatic leukemia (ALL). A Nordic quality assurance program, aimed on standardization of the flow cytometry MRD analysis, has been established before implementation of MRD at cutoff level 10 as one of stratifying parameters in next Nordic Society of Pediatric Hematology and Oncology (NOPHO) treatment program for ALL. In 4 quality control (QC) rounds 15 laboratories determined the MRD levels in 48 follow-up samples from 12 ALL patients treated according to NOPHO 2000. Analysis procedures were standardized. For each QC round a compact disc containing data in list-mode files was sent out and results were submitted to a central laboratory. At cutoff level 10, which will be applied for clinical decisions, laboratories obtained a high concordance (91.6%). If cutoff level 10 was applied, the concordance would be lower (85.3%). The continuing standardization resulted in better concordance in QC3 and QC4 compared with QC1 and QC2. The concordance was higher in precursor B as compared with T-cell ALL. We conclude that after standardization, flow cytometry MRD detection can be reliably applied in international, multicenter treatment protocols.
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| 4. |
- Blomberg, Jeanette, 1977-, et al.
(författare)
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Reduced FAS transcription in clones of U937 cells that have acquired resistance to Fas-induced apoptosis
- 2009
-
Ingår i: The FEBS Journal. - : Wiley. - 1742-464X .- 1742-4658. ; 276:2, s. 497-508
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Tidskriftsartikel (refereegranskat)abstract
- Susceptibility to cell death is a prerequisite for the elimination of tumour cells by cytotoxic immune cells, chemotherapy or irradiation. Activation of the death receptor Fas is critical for the regulation of immune cell homeostasis and efficient killing of tumour cells by apoptosis. To define the molecular changes that occur during selection for insensitivity to Fas-induced apoptosis, a resistant variant of the U937 cell line was established. Individual resistant clones were isolated and characterized. The most frequently observed defect in the resistant cells was reduced Fas expression, which correlated with decreased FAS transcription. Clones with such reduced Fas expression also displayed partial cross-resistance to tumour necrosis factor-alpha stimulation, but the mRNA expression of tumour necrosis factor receptors was not decreased. Reintroduction of Fas conferred susceptibility to Fas but not to tumour necrosis factor-alpha stimulation, suggesting that several alterations could be present in the clones. The reduced Fas expression could not be explained by mutations in the FAS coding sequence or promoter region, or by silencing through methylations. Protein kinase B and extracellular signal-regulated kinase, components of signalling pathways downstream of Ras, were shown to be activated in some of the resistant clones, but none of the three RAS genes was mutated, and experiments using chemical inhibitors could not establish that the activation of these proteins was the cause of Fas resistance as described in other systems. Taken together, the data illustrate that Fas resistance can be caused by reduced Fas expression, which is a result of an unidentified mode of regulation.
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| 5. |
- Bäckman, Karin, et al.
(författare)
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Vårdens alltför svåra val? : kartläggning av prioriteringsarbete och analys av riksdagens principer och riktlinjer för prioriteringar i hälso- och sjukvården
- 2007
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- PrioriteringsCentrum har på uppdrag av Socialstyrelsen genomfört en kartläggning av på vilket sätt hälso- och sjukvårdens huvudmän och andra centrala aktörer arbetar med prioriteringar och har utvärderat hur detta arbete överensstämmer med intentionerna i riksdagens beslut om prioriteringar. Vi har även analyserat innehållet i och tillämpningen av riksdagens riktlinjer för prioriteringar i hälso- och sjukvården. Det har skett genom en etisk analys och mot bakgrund av ett stort antal intervjuer i landsting och kommuner samt med representanter för statliga myndigheter och yrkesorganisationer och med ledning av vad som framkommit i tidigare uppföljningar. Vi föreslår i rapporten ett anta förändringar och förtydliganden av riktlinjerna.Vi kan konstatera att sättet att arbeta med prioriteringar i landsting och kommuner inte är helt olikt det som gällde när Prioriteringsdelegationen redovisade en motsvarande uppföljning år 2001. Fortfarande finns knappast några öppna beslut om fördelning och prioritering av resurser om man med öppenhet avser att beslutsfattaren medvetet överväger flera alternativ och att grunderna för besluten är kända för dem som önskar ta del av dem.I situationer då tillgängliga resurser inte befinner sig i paritet med önskvärda ambitioner får sjukvårdspersonalen ta det största ansvaret för att besluta om och genomföra ransonering av vården. Förutom på chefsnivå tycks dock sjukvårdpersonal fortfarande i liten utsträckning vara medveten om de etiska principer som enligt riksdagsbeslutet ska styra prioriteringar i vården. Få känner till den etiska plattformen med de tre etiska principerna. Lokala mallar eller styrdokument för prioriteringar är ovanliga. Det saknas nödvändiga förutsättningar för att tillämpa riksdagens prioriteringsbeslut och det finns inte heller några tydliga strategier för hur man vill skapa sådana förutsättningar inom landstingen.Den kommunala vård- och omsorgsverksamheten upplever sig fortfarande i ringa utsträckning berörd av den etiska plattformen och prioriteringsprinciperna. Någon gemensam prioritering mellan huvudmännen sker knappast alls.Medborgarna är i mycket liten utsträckning involverade i prioriteringsarbetet. Den ökade öppenheten gentemot brukare innebär oftast att viss information om prioriteringar sker genom traditionella kanaler som patientorganisationer, pensionärsråd och handikappråd och synpunkter inhämtas via allmänna patientenkäter medan klagomål hanteras genom patientnämnder.Vi har också funnit tydliga skillnader när det gäller hur arbetet med prioriteringar bedrivs idag jämfört med för sex år sedan. Genom Socialstyrelsen och Läkemedelsförmånsnämnden har staten tagit ledningen när det gäller att visa hur prioriteringar kan göras på ett systematiskt och öppet sätt. Detta arbete har resulterat i en tydlig metodutveckling. Idag finns det dessutom flera exempel på konkret utvecklingsarbete och samverkan mellan huvudmän kring det vidare begreppet kunskapsstyrd vård till vilket systematiska prioriteringar är starkt relaterat. Vi kan också notera olika initiativ till vertikala prioriteringar i verksamheten där det framförallt är läkarkåren som engagerat sig; men också enstaka försök med systematiska politiska prioriteringar. Det finns dessutom flera lovande utvecklingsprojekt rörande prioriteringar som initierats av och drivs av sjukvårdspersonal både lokalt och nationellt. Yrkesförbunden är också mer aktiva idag när det gäller att sprida kunskap om prioriteringar....
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| 6. |
- Fernaeus, Ylva, et al.
(författare)
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Are we living in a robot cargo cult?
- 2009. - 8
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Ingår i: HRI '09 Proceedings of the 4th ACM/IEEE international conference on Human robot interaction. - New York, NY, USA : ACM. - 9781605584041
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Konferensbidrag (refereegranskat)abstract
- We use the Cargo Cult metaphor to discuss visions, methods and communication of robot research. Essentially cargo cult involves performing of imitative rituals that are conducted without understanding the underlying cause of a phenomenon. We discuss how this is an ongoing challenge within the field of HRI, and what researchers could do to avoid contributing to a robotic cargo cult.
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| 7. |
- Fernaeus, Ylva, et al.
(författare)
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Are We Living in a Robot Cargo Cult?
- 2009
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Ingår i: Proceedings of the 4th ACM/IEEE international conference on Human robot interaction. - La Jolla, California, USA.
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Konferensbidrag (refereegranskat)
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| 8. |
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| 9. |
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| 10. |
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| 11. |
- Gustafsson, Erik, et al.
(författare)
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Invasive Staphylococcus aureus strains are highly variable in PFGE patterns, agr group and exoprotein production
- 2009
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Ingår i: Scandinavian Journal of Infectious Diseases. - : Informa Healthcare. - 0036-5548 .- 1651-1980. ; 41:8, s. 577-583
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Tidskriftsartikel (refereegranskat)abstract
- In the present study, we have investigated 37 invasive Staphylococcus aureus strains (collected between 1997 and 2005) from 33 human episodes of septicaemia causing either endocarditis or vertebral osteomyelitis. All S. aureus strains were typed using pulsed field gel electrophoresis (PFGE), and most strains belonged to any of 4 different PFGE clusters. There was no correlation between any of the PFGE clusters with site of infection. All strains showed highly different expression patterns of extracellular proteins, i.e. we found a vast variation in the number of proteins and amount of individual proteins expressed by the different strains. There was no correlation between any cluster of exoprotein patterns with endocarditis or with vertebral osteomyelitis. We did not find any correlation between agr group and endocarditis, as previously reported. On the other hand, a correlation between some of the PFGE clusters with a certain agr group was found. Known risk factors for S. aureus infections were observed in a majority of the patients.
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| 12. |
- Holmberg, Lars I, 1944-
(författare)
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Health, Risk-Taking Behavior and Sexuality in Swedish Adolescents
- 2007
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The overall aims of this research were to develop methods of identifying adolescents with unhealthy and/or risk-taking behavior with special reference to sexuality, and to evaluate support measures for young people in need of such interventions. A further aim was to assess strategies for preventing unhealthy and/or risk-taking behavior. Data were obtained by questionnaires (studies I, III, IV and V) and interviews (study II).The results showed that young men involved in unintended pregnancies would benefit from active participation in the decision making regarding continuation or termination of the pregnancy, and from support at Outpatient Clinics for Adolescents in Sweden, including information together with the partner (I).It was also concluded that consideration should be paid to questions and problems, e.g. feelings, apprehensions, moral issues and psychosocial factors, that require individual support from personnel with knowledge and resources to help young men in this difficult situation (II).Among the most important findings regarding young men who had been involved in an unplanned pregnancy were that in this group a high percentage had previously considered suicide and that anabolic steroids were frequently used, compared with young men without experience of pregnancy (III).The finding that unprotected intercourse occurred in a fairly high frequency among 13- to 18-year-olds, despite massive education and easy access to contraceptives, gives reason for further considerations regarding appropriate ways of providing information to young people (IV).Girls and boys in vocational programs in Swedish high schools exhibit more risk-taking behaviors than those in theoretical programs and these two groups differ in clustering and accumulation of these behaviors. This means that preventive interventional strategies need to be elaborated with regard to these differences, with the aim of improving health maintenance among adolescents (V).Child-and-youth centers with a developmental and research capacity need to be established with the broad purpose of drawing up health prevention programs for children and young people.
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| 13. |
- Håkansson, Maria, et al.
(författare)
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Facilitating Mobile Music Sharing and Social Interaction with Push!Music
- 2007
-
Ingår i: Proceedings of the 40th Hawaii International Conference on System Sciences. - Los Alamitos, Calif. : IEEE Computer Society Washington. - 1530-1605. - 0769527558 ; , s. 87-
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Konferensbidrag (refereegranskat)abstract
- Push!Music is a novel mobile music listening and sharing system, where users automatically receive songs that have autonomously recommended themselves from nearby players depending on similar listening behaviour and music history. Push!Music also enables users to wirelessly send songs between each other as personal recommendations. We conducted a two-week preliminary user study of Push!Music, where a group of five friends used the application in their everyday life. We learned for example that the shared music in Push!Music became a start for social interaction and that received songs in general were highly appreciated and could be looked upon as 'treats'.
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| 14. |
- Jacobsson, G., et al.
(författare)
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Outcome for invasive Staphylococcus aureus infections
- 2008
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Ingår i: European Journal of Clinical Microbiology and Infectious Diseases. - : Springer. - 0934-9723 .- 1435-4373. ; 27:9, s. 839-848
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Tidskriftsartikel (refereegranskat)abstract
- We report a survey of invasive Staphylococcus aureus (ISA) infections concerning outcome variables such as mortality, recurrence and residual symptoms. A prospective, population-based study of all cases of ISA was conducted in the catchment area of Skaraborg Hospital (population 255,109) in western Sweden during the period from 1st March 2003 to 28th February 2005. One hundred and fifty-seven patients were included. Recurrences were seen in 13 cases (9.3%). Thirty patients (19.1%) died during the first 28 days. Mortality rates for complicated bacteraemia and severe sepsis were 32% and 54%, respectively. Older patients (> 65 years of age), patients with concomitant heart disease and patients with endovascular infections all suffered higher mortality. Line-associated infections had a higher recurrence rate. Residual symptoms were common, with 34% of the living patients reporting incomplete recovery. Accessory gene regulator (agr) type within the bacteria did not affect disease presentation. We conclude that ISA infections are of major medical importance, with high rates of mortality (19.1%), recurrence (9.3%) and residual functional impairment (34%).
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| 15. |
- Jacobsson, Kerstin, et al.
(författare)
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A sociology of scandal and moral transgression : the Swedish ’ Nannygate ’ scandal
- 2008
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Ingår i: Acta Sociologica. - : SAGE. - 0001-6993 .- 1502-3869. ; 51:3, s. 203-216
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Tidskriftsartikel (refereegranskat)abstract
- In this article, we use the case of a government scandal in Sweden to develop a theoretical perspective by which to understand the moral nature of political scandals. We draw on insights from Durkheim's sociology of morality and point, inter alia, to the ritual character of scandals. However, in contrast to most Durkheimian readings, the perspective presented does not presume the existence of a moral consensus. The scandal is understood as a confrontation between various systems of norms. Rather than confirming a given moral order, scandals provoke moral positioning and help in clarifying — and dramatizing — lines of difference or conflict. The empirical case studied is a government scandal in Sweden in 2006 (Nannygate) that forced two government ministers to resign after less than 10 days in office when it was revealed that they had not paid their TV licence and, moreover, had bought `black' (untaxed) services. This transgression provoked a massive public reaction at the time.
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| 16. |
- Jacobsson, Kerstin, et al.
(författare)
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En expertorganisation ändrar uppfattning: Om OECDs omsvängning i arbetsmarknadspolitiken
- 2008
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Ingår i: Arbetsmarknad & Arbetsliv. - Karlstad : Karlstads universitet. - 1400-9692 .- 2002-343X. ; 14:3, s. 31-43
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Tidskriftsartikel (refereegranskat)abstract
- I 30 år har OECD trummat ut sitt budskap om behovet av strukturreformer på arbetsmarknaden. Receptet har varit likartat oavsett patient och ofta tycks förskrivningen ha skett enligt devisen ”ju beskare medicin, desto trovärdigare behandling”. Många blev därför förvånade när OECD, efter en ambitiös översyn av sin jobbstrategi, år 2006 gav upp tanken om en enda gyllene reformväg. Artikeln pekar på viktiga förändringar i den reviderade jobbstrategin och analyserar vilka faktorer som låg bakom OECD:s omsvängning i arbetsmarknadspolitiken.
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| 17. |
- Jacobsson, Mattias, et al.
(författare)
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GlowBots : Designing and Implementing Engaging Human-Robot Interaction
- 2008
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Ingår i: Journal of Physical Agents. - 1888-0258. ; 2:2, s. 51-60
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Tidskriftsartikel (refereegranskat)abstract
- GlowBots are small tangible, communicating and interactive robots that show eye-catching visual patterns on a round LED display. This paper details the development of the GlowBots from the early user-oriented design phase, through hardware and software development and onto preliminary user studies. In the design phase we outlined a robot application based on a study of how owners relate with unusual pets, such as snakes and lizards. This led to an application concept of a set of ”hobby robots” which would communicate with each other and the user through dynamic patterns. Based on these requirements, we developed a LED display called see- Puck, which together with an open robot platform was used for the GlowBots application itself. One particular issue is dealing with energy consumption problems, as resources in embedded systems often limit the potential time for user interaction. We conclude with a report on early user experiences from demonstrating GlowBots and a preliminary user study in a home environment as well as remarks about future directions.
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| 18. |
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| 19. |
- Jacobsson, Mattias, et al.
(författare)
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GlowBots: Robots that Evolve Relationships
- 2007. - 1
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Konferensbidrag (refereegranskat)abstract
- GlowBots are small wheeled robots that develop complex relationships between each other and with their owner. They develop attractive patterns which are affected both by user interaction and communication between the robots. The project shows how robots can interact with humans in subtle and sustainable ways for entertainment and enjoyment.
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| 20. |
- Jacobsson, Markus, et al.
(författare)
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Love and authentication
- 2008
-
Ingår i: Proceedings of ACM CHI 2008 Conference on Human Factors in Computing Systems. ; , s. 197-200
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Konferensbidrag (refereegranskat)
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| 21. |
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| 22. |
- Jacobsson, Mattias, et al.
(författare)
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The see-Puck: a platform for exploring human-robot relationships
- 2008. - 1
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Ingår i: ACM Conference on Human Factors in Computing Systems.
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Konferensbidrag (refereegranskat)abstract
- We present the see-Puck, a round display module that extends an open robot platform, the e-Puck. It holds 148 LEDs (light emitting diodes) to enable the presentation of eye-catching visual animated patterns, while keeping hardware costs and energy consumption at a minimum. The see-Puck was a result of a study of future robot applications, where relationship and interaction qualities found in owners of unusual pets (e.g. spiders, snakes, and lizards) were transferred to the robotic domain. In our first proof-of-concept application, humans and robots can engage in a playful open ended interaction. We argue that open interactive robot platforms such as the see-Puck point to opportunities not only in robotics but also future user interfaces and ubiquitous computing.
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| 23. |
- Jacobsson, Mattias, et al.
(författare)
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When media gets wise: Collaborative filtering with mobile media agents
- 2006
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Ingår i: International Conference on Intelligent User Interfaces, Proceedings IUI. - New York, NY, USA : ACM. - 1595932879
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Konferensbidrag (refereegranskat)abstract
- We present a model where media (e.g. music files) are autonomous entities that carry their own individual information. Our goal is to turn such files into autonomous, rule-following agents capable of building their own identities from interactions with other agents and users, We are exploring how collaborative filtering-like behaviour could emerge out of large ensembles of interacting agents, which are distributed over mobile devices in social networks. We have implemented a first version of the model in the form of a music player application for mobile devices, called Push!Music. This system takes advantage of active recommendations as well as implicit user activity to build a profile for each media file.
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| 24. |
- Johansson, Patrik, 1969, et al.
(författare)
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A novel field of ab initio studies: complexation of simple anions within neutral cryptands
- 2005
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Ingår i: Journal of Molecular Structure: THEOCHEM. - : Elsevier BV. - 0166-1280. ; 717:1-3, s. 215-221
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Tidskriftsartikel (refereegranskat)abstract
- We present here an ab initio study on anion guest - macrocycle host relationships. The complexation of different simple inorganic anions (F - , Cl - , BF 4 - and ClO 4 - ) with the neutral cryptand, the macrotricycle (H 3 BN) 4 [(CH 2 ) n ] 6 , (n=3-6), has been studied using ab initio Hartree-Fock calculations and density functional theory using the B3LYP hybrid functional. As the side-lengths of the pure cryptands increase with the oligomeric sidechain (n), the cryptand cavity increases as n 3 . F - is the most strongly preferred anion for all cryptands, only for the largest host cavity (n=6) complexes are formed exothermally for all anion guests. Morokuma-Kitaura decomposition has been performed to analyse the total interaction energies within the created supermolecules in physically relevant terms including the importance of treatment of the basis set superposition error. According to this scheme the polarisation/charge transfer parts differ in importance amongst the complexes in facilitating a strong binding of the anion guests, but the dominant balance being that of Coulombic attraction and Pauli repulsion. © 2004 Elsevier B.V. All rights reserved.
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| 25. |
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| 26. |
- Lönnerholm, Gudmar, et al.
(författare)
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In vitro cellular drug sensitivity at diagnosis is correlated to minimal residual disease at end of induction therapy in childhood acute lymphoblastic leukemia.
- 2009
-
Ingår i: Leukemia research. - Oxford : Elsevier BV. - 0145-2126 .- 1873-5835. ; 33:1, s. 46-53
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Tidskriftsartikel (refereegranskat)abstract
- Leukemic cells from 85 children with newly diagnosed precursor B-lineage ALL were tested for in vitro drug sensitivity to a panel of anti-cancer drugs. Minimal residual disease (MRD) was measured by RQ-PCR. There was a significant correlation between MRD day 29 and in vitro sensitivity to prednisolone (p<0.001) and doxorubicin (p=0.017), drugs administered during induction therapy. In patients with t(12;21) (n=20), in vitro sensitivity to doxorubicin was an independent factor for MRD <0.1% (p=0.031; R(2)=0.66). Thus, data show that in vitro drug sensitivity at diagnosis is correlated to cell kill during induction therapy as measured by MRD day 29.
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| 27. |
- Petruson, Karin, 1967, et al.
(författare)
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Nitric oxide production in the sphenoidal sinus by the inducible and constitutive isozymes of nitric oxide synthase
- 2005
-
Ingår i: Rhinology. - 0300-0729. ; 43:1, s. 18-23
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To study the production of nitric oxide (NO), and the presence of different isoforms of the NO-synthesising enzyme, NO-synthase (NOS), in the paranasal sinus. MATERIALS AND METHODS: Ten patients, undergoing surgery for pituitary adenoma, were examined for the presence of NO gas in the sphenoidal and maxillary sinus. The distribution of different NOS isozymes in mucosal biopsies from sphenoid and maxillary sinus and ethmoidal cells was studied. RESULTS: The mean concentration of NO was 2575 ppb in the sphenoidal sinus and 6792 ppb in the maxillary sinus. Morphological analyses revealed intense NADPH-diaphorase staining throughout the epithelium. Immunoreactivity against NOS2 (inducible NOS) was observed in the apical cell layer but not of the basal layer. NOS1 (neuronal NOS)-immunoreactivity was mainly seen in the subapical part of the epithelium and NOS3 (endothelial NOS)-immunoreactivity was observed only in the most apical part of the epithelium. CONCLUSION: NO concentration in the sphenoidal sinus is about the same as in the nasal cavity and approximately half of the concentration found in the maxillary sinus. All of the three main different isozymes of NOS can be demonstrated in the mucosa of the sphenoidal and maxillary sinus and ethmoidal cells, NOS2 being the most abundant isoform.
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| 28. |
- Pettersson, Erik, et al.
(författare)
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Allelotyping by massively parallel pyrosequencing of SNP-carrying trinucleotide threads
- 2008
-
Ingår i: Human Mutation. - : Hindawi Limited. - 1059-7794 .- 1098-1004. ; 29:2, s. 323-329
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Tidskriftsartikel (refereegranskat)abstract
- Here we present an approach for allelotyping combining the multiplexing features of the trinucleotide threading (TnT) method with pooling of genomic DNA and massively parallel pyrosequencing, enabling reliable allele frequency estimation in large cohorts. The approach offers several benefits as compared to array-based methods and allows undertaking highly complex studies without compromising accuracy, while keeping the workload to a minimum. This proof-of-concept study involves formation of trinucleotide threads, targeting a total of 147 single-nucleotide polymorphisms (SNPs) related to obesity and cancer, for multiplex amplification and allele extraction from a pool of 462 genomes, followed by massively parallel pyrosequencing. Approximately 177k reads were approved, identified, and assigned to SNP-carrying threads rendering representative allele frequencies in the cohort.
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| 29. |
- Ryberg, Martin, 1976, et al.
(författare)
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Mining metadata from unidentified ITS sequences in GenBank: a case study in Inocybe (Basidiomycota)
- 2008
-
Ingår i: BMC Evolutionary Biology. - : Springer Science and Business Media LLC. - 1471-2148. ; 8:50
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Tidskriftsartikel (refereegranskat)abstract
- Background The lack of reference sequences from well-identified mycorrhizal fungi often poses a challenge to the inference of taxonomic affiliation of sequences from environmental samples, and many environmental sequences are thus left unidentified. Such unidentified sequences belonging to the widely distributed ectomycorrhizal fungal genus Inocybe (Basidiomycota) were retrieved from GenBank and divided into species that were identified in a phylogenetic context using a reference dataset from an ongoing study of the genus. The sequence metadata of the unidentified Inocybe sequences stored in GenBank, as well as data from the corresponding original papers, were compiled and used to explore the ecology and distribution of the genus. In addition, the relative occurrence of Inocybe was contrasted to that of other mycorrhizal genera. Results Most species of Inocybe were found to have less than 3% intraspecific variability in the ITS2 region of the nuclear ribosomal DNA. This cut-off value was used jointly with phylogenetic analysis to delimit and identify unidentified Inocybe sequences to species level. A total of 177 unidentified Inocybe ITS sequences corresponding to 98 species were recovered, 32% of which were successfully identified to species level in this study. These sequences account for an unexpectedly large proportion of the publicly available unidentified fungal ITS sequences when compared with other mycorrhizal genera. Eight Inocybe species were reported from multiple hosts and some even from hosts forming arbutoid or orchid mycorrhizae. Furthermore, Inocybe sequences have been reported from four continents and in climate zones ranging from cold temperate to equatorial climate. Out of the 19 species found in more than one study, six were found in both Europe and North America and one was found in both Europe and Japan, indicating that at least many north temperate species have a wide distribution. Conclusions Although DNA-based species identification and circumscription are associated with practical and conceptual difficulties, they also offer new possibilities and avenues for research. Metadata assembly holds great potential to synthesize valuable information from community studies for use in a species and taxonomy-oriented framework.
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| 30. |
- Svensson, Jenny, 1977-
(författare)
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The Regulation of Rule-Following : Imitation and Soft Regulation in the European Union
- 2009
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Present times are sometimes referred to as "the golden era of regulation", as more and more areas of social life are regulated. But regulation is not only increasing; it is also changing. New regulators are emerging, and they are issuing new kinds of rules. These new kinds of regulation are frequently not legally binding, and are therefore labelled soft regulation as opposed to hard law. It is not compulsory to follow soft rules but many actors - including sovereign states - still do, and the thesis asks the question why this is so. Why do even states, which are powerful regulators themselves, abide by soft regulation, and wherein lies the regulative power of soft rules? Through an in-depth study of the European Union's pre-accession instrument Twinning an answer to the question of the power of soft regulation has been arrived at. Treating Twinning as a critical case of soft regulation, and using theories of imitation to grasp the meaning and evolution of Twinning projects, makes it possible to define three regulative elements involved in soft regulation. These are the combinative, co-productive and constitutive elements of soft regulation, from which the thesis suggests that it derives its power. First of all, soft regulation combines different kinds of rules, the regulation of identity and the regulation of activity, and a variety of sources of legitimacy. Second, it depends on regulators and regulatees interacting to co-produce regulation. And third, as its main result, it constitutes the rule-followers as formal, rational, and modern organisations. Accordingly, soft regulation has rather impressive regulative capabilities, builds on complex, dynamic, and social interactions, and embodies as well as promotes some of Western society's most strongly institutionalised ideas. The thesis argues that it is through these characteristics that actors, including states, are compelled to follow soft rules.
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- Thörn, Ingrid, 1957-, et al.
(författare)
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Minimal residual disease assessment in childhood acute lymphoblastic leukemia : Results of a Swedish multi-centre study comparing real-time PCR and multi-colour flow cytometry
- 2009
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- In this Swedish multi-center study of early treatment response in childhood acute lymphoblastic leukemia (ALL), we evaluated the concordance between multicolour flow cytometry (FCM) and real-time quantitative polymerase chain reaction (RQ-PCR) for assessment of minimal residual disease (MRD). Multiple time points (i.e. day 15, 29, 50 and 106) were evaluated with the NOPHO (Nordic Society of Pediatric Hematology and Oncology) ALL 2000 treatment protocol as backbone. During 2002-2006, 334 children were diagnosed with ALL, where 228 had paired samples taken at any of the four time points. With the detection level of 0.1%, the concordance between RQ-PCR and FCM was 90% in the 726 paired samples analyzed. At day 29, the correlation between the methods was greater with MRD levels >0.1% (rs=0.7, p<0.001) than below (rs=0.2, p=0.024). MRD levels higher than 0.1% at day 29 was a significant predictor of higher risk of having a bone marrow relapse. This was true both for BCP ALL and T-ALL analysed with either FCM or RQ-PCR, although RQ-PCR was a better discriminator than FCM in T-ALL. However, using the NOPHO ALL 2000 protocol, our data indicate that a higher cut-off value (0.2%) should be applied in BCP ALL when using RQ-PCR as MRD method. In contrast, MRD levels ≥ 0.1%, analysed with either method late during induction therapy, was not a predictor of isolated extramedullary relapse. We therefore conclude that MRD assessment by RQ-PCR based IG/TCR rearrangement and multicolour FCM monitoring can be used as a clinical tool if the aim is to find childhood ALL cases with increased risk of having bone marrow relapses.
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