| 1. |
- Ahlman, Håkan, 1947, et al.
(författare)
-
Adrenocortical carcinoma--diagnostic and therapeutical implications.
- 1993
-
Ingår i: The European journal of surgery = Acta chirurgica. - 1102-4151. ; 159:3, s. 149-58
-
Tidskriftsartikel (refereegranskat)abstract
- To evaluate the results of treatment of a consecutive series of patients with adrenocortical carcinoma who presented during the six year period 1985 to 1991.
|
|
| 2. |
|
|
| 3. |
- Brabant, G, et al.
(författare)
-
E-cadherin: a differentiation marker in thyroid malignancies.
- 1993
-
Ingår i: Cancer research. - 0008-5472. ; 53:20, s. 4987-93
-
Tidskriftsartikel (refereegranskat)abstract
- Loss of E-cadherin (uvomorulin), a Ca(2+)-dependent cell adhesion molecule required for normal epithelial function, has been attributed a pathogenetic role in tumor invasion. The expression of E-cadherin was studied in normal and neoplastic follicular epithelium of the human thyroid by Northern blot analysis and immunofluorescence on frozen tissue sections. In the normal thyroid (n = 10) and in benign thyroid disorders (n = 21; toxic diffuse goitre; multinodular goitre; follicular adenomas), E-cadherin mRNA levels were equally high and the follicles were generally stained, mainly along the lateral surface of the epithelial cells, by the anti-E-cadherin monoclonal antibody. In anaplastic thyroid carcinomas (n = 6) E-cadherin expression was very low or lacking. In papillary carcinomas (n = 23), E-cadherin mRNA levels varied from nearly normal to highly reduced, which roughly correlated with the overall immunofluorescence intensity. However, the immunostaining also revealed a heterogeneous "all-or-nothing" expression of E-cadherin among adjacent cells in the same tumor. In the follicular carcinomas (n = 9), E-cadherin mRNA levels were in general rather high but the immunostaining varied considerably. A few papillary and follicular tumors lacked immunoreactive E-cadherin in spite of high mRNA levels. In oxyphilic (Hürthle) cell tumors, comprising both adenomas (n = 4) and carcinomas (n = 2), E-cadherin immunoreactivity was reduced and distributed intracellularly rather than at the cell surface. The expression of E-cadherin in relapsing thyroid carcinomas and in tumors with metastatic spreading was, irrespective of the histiotype, low or lacking. Sequential Northern analysis revealed a close correlation between the expression levels of E-cadherin and the thyrotropin receptor. Together, the data suggest that in human thyroid malignancies both gene expression and posttranscriptional control of E-cadherin may be impaired.
|
|
| 4. |
- Esbjörnsson, M, et al.
(författare)
-
Muscle fibre types and enzyme activities after training with local leg ischaemia in man.
- 1993
-
Ingår i: Acta Physiologica Scandinavica. - : Wiley. - 0001-6772 .- 1365-201X. ; 148:3, s. 233-41
-
Tidskriftsartikel (refereegranskat)abstract
- Eight healthy men performed supine one-legged training on a bicycle ergometer 45 min per leg four times per week for 4 week. The ergometer and lower body were inside a pressure chamber, the opening of which was sealed at the level of the crotch. One leg trained with impeded leg blood flow (I-leg), induced by an increased (50 mmHg) chamber pressure, at the highest tolerable intensity. The contralateral leg trained at the same power under normal pressure (N-leg). Before and after training biopsies were taken from the vastus lateralis of both legs and maximal one-legged exercise tests were executed with both legs. Biopsies were repeated when the subjects had been back to their habitual physical activity for 3 months. Training increased exercise time to exhaustion, but more in the I-leg than in the N-leg. After training, the I-leg had higher activity of citrate synthase (CS), a marker of oxidative capacity, and lower activity of the M-subunit of lactate dehydrogenase isoenzymes. It also had a higher percentage of type-I fibres and a lower percentage of IIB fibres, larger areas of all fibre types and a greater number of capillaries per fibre. It is concluded that ischaemic training changes the muscle metabolic profile in a direction facilitating aerobic metabolism. An altered fibre-type composition may contribute, but is not enough prerequisite for the change.
|
|
| 5. |
- Fu, Michael, 1963, et al.
(författare)
-
Increase in functional activity rather than in amount of Gi-alpha in failing human heart with dilated cardiomyopathy.
- 1992
-
Ingår i: Cardiovascular research. - 0008-6363. ; 26:10, s. 950-5
-
Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: The aim was to investigate whether or not increased pertussis toxin catalysed ADP ribosylation correlates with increased amount of Gi-alpha in failing human heart. DESIGN: Antisera raised against unique synthetic peptides corresponding to alpha subunits of Gs and Gi 1-3 were used in immunoblotting and ELISA to determine amounts of various G proteins. Adenylyl cyclase activity, beta adrenoceptors, and muscarinic receptors were then measured in cardiomyopathic hearts (n = 6) obtained at transplant in order to study whether or not an altered expression of G proteins has relevance to the integrity and function of the receptor--adenylyl cyclase system. Six non-failing control hearts were also studied. RESULTS: No significant differences in the peptide equivalent amounts of either Gs or Gi were found in the failing human heart as compared to the non-failing heart. However, functional activity of Gi was shown to increase significantly since there was a decrease in basal (57%), isoprenaline stimulated (60%), and guanyliminodiphosphate stimulated (52%) adenylyl cyclase activity. In contrast the density of beta adrenoceptors was markedly decreased (51%) in failing human heart in comparison to non-failing hearts. Neither the density nor the affinity of muscarinic receptors changed in the failing human heart. CONCLUSION: These results suggest that in the failing human heart, there is an increase in functional activity rather than in amount of Gi, and an important part of functional expression of Gi-alpha may be regulated at the post-translational level.
|
|
| 6. |
|
|
| 7. |
- Jansson, M, et al.
(författare)
-
Effects of luminal stimuli on polyamine metabolism in the small intestine of the rat : the role of enteric nerves
- 1993
-
Ingår i: Acta Physiologica Scandinavica. - : Wiley. - 0001-6772 .- 1365-201X. ; 149:4, s. 90-483
-
Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to investigate to what extent polyamine metabolism in the small intestine of the rat is controlled by the enteric nervous system. Polyamine metabolism was followed by measuring the activity of ornithine decarboxylase (ODC) and in some instances also the content of polyamines (putrescine, spermidine and spermine). ODC activity in the intestine was increased when intraluminal pressure was increased and 3 h after placing cholera toxin in the intestinal lumen. Cholera toxin also increased the tissue putrescine content. Atropine or hexamethonium given i.v. did not influence the evoked changes of ODC activity. The pressure induced changes were not decreased by placing lidocaine on the serosal surface. On the other hand, the ODC activity of control segments were decreased by hexamethonium or atropine. The presence of glucose in the intestinal perfusate did not augment tissue ODC activity, neither did the heat stable enterotoxin from Escherichia coli (STa). It is concluded that the effect on polyamine metabolism evoked by luminal pressure or cholera toxin seems not to be mediated via nerves, while nerves seem to influence ODC activity during control conditions. The experiments with enterotoxins suggest that cAMP is the intracellular second messenger controlling intestinal ODC activity.
|
|
| 8. |
- Kaijser, L., et al.
(författare)
-
Muscle oxidative capacity and work performance after training under local leg ischemia
- 1990
-
Ingår i: Journal of applied physiology. - : American Physiological Society. - 8750-7587 .- 1522-1601. ; 69:2, s. 785-787
-
Tidskriftsartikel (refereegranskat)abstract
- Healthy young men executed supine one-legged cycle training four times per week for 4 wk with legs and the cycle ergometer inside a pressure chamber, the opening of which was sealed by a rubber membrane at the level of the crotch. Each training session started by training one leg under ischemic conditions induced by increased chamber pressure (50 mmHg) at the highest intensity tolerable for 45 min. Then the other leg was trained with the same power profile but normal atmospheric chamber pressure. Before and after the training period, both legs executed one-legged exercise tests under both normal and increased chamber pressure and muscle biopsies were taken from the vastus lateralis. Ischemic training increased performance more than normal training, the difference being greater for exercise executed under ischemic conditions. The difference in performance increase between the legs was paralleled by a greater muscle citrate synthase activity in the ischemically than in the normally trained leg.
|
|
| 9. |
- Ledin, T., et al.
(författare)
-
Dynamic posturography in psychoorganic syndrome
- 1991
-
Ingår i: Vertigo, nausea, tinnitus and hypoacusia due to head and neck trauma. - Amsterdam : Excerpta Medica. - 0444811508 ; , s. 367-369
-
Konferensbidrag (refereegranskat)
|
|
| 10. |
|
|
| 11. |
- Ljungberg, Ulla K, et al.
(författare)
-
The interaction between different domains of staphylococcal protein A and human polyclonal IgG, IgA, IgM and F(ab')2: separation of affinity from specificity
- 1993
-
Ingår i: Molecular Immunology. - 1872-9142. ; 30:14, s. 1279-1285
-
Tidskriftsartikel (refereegranskat)abstract
- Binding properties of staphylococcal protein A (SpA) to different human immunoglobulins have been investigated. In this analysis, intact SpA as well as SpA-derived fragments containing one to five IgG-binding domains of different compositions, were used. The affinity binding constants of the different proteins to human polyclonal IgG, IgA, IgM and F(ab')2-fragments as well as their binding capacity to the immunoglobulin molecules were determined. The results show that although all the proteins bound to IgG, regardless of size or composition, the binding strength differed significantly. Proteins containing five domains have a stronger affinity for IgG than those containing one or two. There were no marked differences in binding strength between different domains. However, the binding ability to IgA and IgM showed a marked difference between the various SpA-derived proteins of different compositions. This discrepancy was correlated to differences in their relative binding properties to isolated F(ab')2-fragments of IgG. Hence, we conclude that the binding affinity is mainly affected by the number of domains, whereas the binding specificity is to a large extent determined by which domains are selected.
|
|
| 12. |
|
|
| 13. |
- Mölne, Johan, 1958, et al.
(författare)
-
Adherence of RFD-1 positive dendritic cells to the basal surface of thyroid follicular cells in Graves' disease.
- 1994
-
Ingår i: Autoimmunity. - 0891-6934. ; 17:1, s. 59-71
-
Tidskriftsartikel (refereegranskat)abstract
- HLA-DR positive cells infiltrating Graves' thyroid tissue were examined for their expression of cell-specific immunological markers using light and electron microscopic immunostaining of frozen sections and isolated open thyroid follicles. Graves' glands (n = 21) were enriched of CD68 and Leu-M5/CD11c positive monocytes/macrophages as well as CD4 and CD8 lymphocytes. These cell types were distributed juxta-follicular as well as in other tissue areas. Only the RFD-1 antibody, considered to label antigen-presenting cells including dendritic cells, identified cells invariably located close to the interstitial follicular surface. After follicle isolation, RFD-1 cells were enriched compared to Leu-M5 cells and exclusively adherent to the follicular epithelium. The plasma membrane of RFD-1 positive cells were in intimate contact with the basolateral membrane of the thyrocytes, sometimes extending deeply into the intercellular space of the epithelium. Parallel labelling experiments suggested that the follicle-adhering RFD-1 cells also expressed HLA-DR. Our findings show that in human thyroid glands with Graves' disease RFD-1 positive cells with a dendritic morphology establish direct contact with the follicular epithelium. In view of the fact that both HLA-DR and RFD-1 are associated with antigen-presenting functions it is suggested that a direct interaction of dendritic cells with thyrocytes might be an important component of the autoimmune reaction in Graves' disease.
|
|
| 14. |
- Mölne, Johan, 1958, et al.
(författare)
-
Non-polarized cell surface expression of HLA-A,B,C and HLA-DR antigens in Graves' thyroid follicle cells.
- 1991
-
Ingår i: Autoimmunity. - 0891-6934. ; 10:3, s. 189-99
-
Tidskriftsartikel (refereegranskat)abstract
- The intrathyroidal distribution and cell surface location of HLA-A,B,C and HLA-DR antigens was studied in polarized thyroid follicle cells from Graves' (n = 11) and normal (n = 3) thyroid tissue, using light and electron microscopy. Cryosections and isolated, open follicle segments were incubated with monoclonal antibodies against HLA-A,B,C and HLA-DR antigens and with patient sera containing autoantibodies against the microsomal antigen/thyroperoxidase. Immunoreactivity for HLA-A,B,C and HLA-DR on isolated thyroid follicle cells was frequently detected in Graves' disease, but absent in normal glands. There was a large variation in the immunolabelling between follicles as well as between different glands. Both HLA-A,B,C and HLA-DR immunoreactivity were detected on the apical and the basal surface of the follicle cells. Microsomal antigen/thyroperoxidase immunoreactivity was restricted to the apical cell surface. In contrast to normal tissue, HLA-DR positive cells with a dendritic or macrophage-like morphology were frequent in Graves' tissue. These cells adhered directly to the basal surface of isolated follicle segments. We conclude that HLA antigens are, unlike thyroid-specific plasma membrane constituents, expressed in a non-polarized manner at the surface of the follicular epithelium. These observations might have implications on the immune recognition of thyroidal autoantigens in Graves' disease.
|
|
| 15. |
- Saldner, Henrik, et al.
(författare)
-
Video demonstration of violin body vibrations
- 1992
-
Ingår i: Proceedings of the International symposium on musical acoustics. - Tokyo : Musical Acoustics Research Group of Acoustical Society of Japan.
-
Konferensbidrag (refereegranskat)
|
|
| 16. |
- Tunlid, Anders, et al.
(författare)
-
Infrared monitoring of the adhesion of Catenaria anguillulae zoospores to solid surfaces
- 1991
-
Ingår i: Experimental Mycology. - : Elsevier BV. - 0147-5975. ; 15:3, s. 206-214
-
Tidskriftsartikel (refereegranskat)abstract
- Electron microscopic studies of nematodes infected with the chytridiomycetous fungusCatenaria anguillulae indicated that zoospores of the fungus adhered to the cuticle of nematodes by a layer of extracellular polymers. The chemical composition of the adhesive polymers and their interaction with a solid surface were examined with Fourier transform infrared spectroscopy, using an attenuated total reflectance cell. On-line monitoring of the adhesion of zoospores to a germanium crystal with this technique showed that the adhesive polymers consisted of a protein(s) containing amide I and II bands. The adsorption of these proteins, measured as the increase in the amide II band, had a rapid initial phase of ca. 20 minutes, followed by a slower increase during the course of incubation. Fluorescein isothiocyanate staining of the attached cells at the end of the experiment showed that the adhesion of the zoospores occurred before the formation of the cyst wall.
|
|
