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- Halldin, S, et al.
(author)
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Continuous long-term measurements of soil-plant-atmosphere variables at an agricultural site
- 1999
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In: AGRICULTURAL AND FOREST METEOROLOGY. - : ELSEVIER SCIENCE BV. - 0168-1923. ; 98-9, s. 75-102
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Journal article (peer-reviewed)abstract
- It is a major challenge in modem science to decrease the uncertainty in predictions of global climate change. One of the largest uncertainties in present-day global climate models resides with the understanding of processes in the soil-vegetation-atmosphe
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- Ekberg-Jansson, A, et al.
(author)
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A comparison of the expression of lymphocyte activation markers in blood, bronchial biopsies and bronchoalveolar lavage: evidence for an enrichment of activated T lymphocytes in the bronchoalveolar space
- 1999
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In: Respiratory Medicine. - 1532-3064. ; 93:8, s. 563-570
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Journal article (peer-reviewed)abstract
- In this study healthy never-smoking subjects (n = 18) were recruited from a population study. Bronchoalveolar lavage (BAL), blood lymphocytes and bronchial biopsies, analysed both in the epithelium and lamina propria, were stained for T and B lymphocytes, natural killer (NK) cells and different subpopulations of T lymphocytes. In BAL, significantly higher proportions of T lymphocytes (CD3), T lymphocyte activation markers; HLA-DR, CD26+, CD49a+, CD54+ and CD69+, helper T (CD3+4+) and memory helper T lymphocytes (CD4+45RO+29+) and memory T lymphocytes (CD3+45RO+) were found, compared to blood. However, the proportion of IL-2 receptor-positive T lymphocytes (CD25+) was lower in BAL than in blood. A previously described higher ratio of CD3+4+/CD3+8+ in BAL than in blood (3.4 vs 1.7; P = 0.001) was confirmed. In bronchial biopsies, we found significantly higher numbers of CD8+ cell profiles per mm2 in the epithelial compared to the lamina propria compartment. We conclude that healthy never-smoking men have higher levels of activated memory T lymphocytes in BAL than in blood, and that the T-cell subpopulations differ in the epithelial compared to the lamina propria compartment in the bronchial mucosa and these compartments should be analysed separately. It is reasonable to think that there is a gradient from blood to the airway lumen where T cells are recruited from blood to take part in the defense towards damaging agents.
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- Esbjornsson-Liljedahl, M, et al.
(author)
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Metabolic response in type I and type II muscle fibers during a 30-s cycle sprint in men and women
- 1999
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In: Journal of applied physiology (Bethesda, Md. : 1985). - : American Physiological Society. - 8750-7587 .- 1522-1601. ; 87:4, s. 1326-1332
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Journal article (peer-reviewed)abstract
- The acute metabolic response to sprint exercise was studied in 20 male and 19 female students. We hypothesized that the reduction of muscle glycogen content during sprint exercise would be smaller in women than in men and that a possible gender difference in glycogen reduction would be higher in type II than in type I fibers. The exercise-induced increase in blood lactate concentration was 22% smaller in women than in men. A considerable reduction of ATP (50%), phosphocreatine (83%), and glycogen (35%) was found in type II muscle fibers, and it did not differ between the genders. A smaller reduction of ATP (17%) and phosphocreatine (78%) was found in type I fibers, and it did not differ between the genders. However, the exercise-induced reduction in glycogen content in type I fibers was 50% smaller in women than in men. The hypothesis was indeed partly confirmed: the exercise-induced glycogen reduction was attenuated in women compared with men, but the gender difference was in type I rather than in type II fibers. Fiber-type-specific and gender-related differences in the metabolic response to sprint exercise might have implications for the design of training programs for men and women.
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- Hankamer, B, et al.
(author)
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Isolation and biochemical characterisation of monomeric and dimeric photosystem II complexes from spinach and their relevance to the organisation of photosystem II in vivo
- 1997
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In: European Journal of Biochemistry. - 0014-2956 .- 1432-1033. ; 243:1-2, s. 422-429
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Journal article (peer-reviewed)abstract
- Membranes enriched in photosystem II were isolated from spinach and further solubilised using n-octyl beta-D-glucopyranoside (OctGlc) and n-dodecyl beta-D-maltoside (DodGlc(2)). The OctGlc preparation had high rates of oxygen evolution and when subjected to size-exclusion HPLC and sucrose density gradient centrifugation, in the presence of DodGlc(2), separated into dimeric (430 kDa), monomeric (236 kDa) photosystem II cores and a fraction containing photosystem II light-harvesting complex (Lhcb) proteins. The dimeric core fraction was more stable, contained higher levels of chlorophyll, beta-carotene and plastoquinone per photosystem II reaction centre and had a higher oxygen-evolving activity than the monomeric cores. Their subunit composition was similar (CP43, CP47, D1, D2, cytochrome b 559 and several lower-molecular-mass components) except that the level of 33-kDa extrinsic protein was lower in the monomeric fraction. Direct solubilisation of photosystem-II-enriched membranes with DodGlc(2), followed by sucrose density gradient centrifugation, yielded a super complex (700 kDa) containing the dimeric form of the photosystem II core and Lhcb proteins: Lhcb1, Lhcb2, Lhcb4 (CP29), and Lhcb5 (CP26). Like the dimeric and monomeric photosystem II core complexes, the photosystem II-LHCII complex had lost the 23-kDa and 17-kDa extrinsic proteins, but maintained the 33-kDa protein and the ability to evolve oxygen. It is suggested, with a proposed model, that the isolated photosystem II-LHCII super complex represents an in vivo organisation that can sometimes form a lattice in granal membranes of the type detected by freeze-etch electron microscopy [Seibert, M., DeWit, M. & Staehelin, L. A. (1987) J. Cell Biol. 105, 2257-2265].
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| 43. |
- Henriksson, Anders E., et al.
(author)
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Experimental haemorrhage and blood component transfusion in humans : no change in plasma concentration of thrombin-antithrombin complex and plasmin-antiplasmin complex.
- 1996
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In: Thrombosis Research. - : Elsevier BV. - 0049-3848 .- 1879-2472. ; 82:5, s. 409-15
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Journal article (peer-reviewed)abstract
- The influence of haemorrhage and blood transfusion on primary haemostasis, coagulation and fibrinolysis was investigated in ten healthy male volunteers. Acute loss of 10% of the blood volume did not give any significant alteration in thrombin- antithrombin III (TAT) complex and plasmin-alpha 2-antiplasmin (PAP) complex levels compared with a control series. The skin bleeding time with the Simplate II device was not altered after the 10% blood loss. Acute loss of 10% of blood volume followed by transfusion of packed red cells or stored plasma did not resulted in any significant change in bleeding time, TAT and PAP complex levels. It could be concluded that a controlled haemorrhage does not give any detectable changes of the platelet dependent primary haemostasis, blood coagulation and fibrinolysis. Transfusion of one unit of packed red cells or stored plasma does not seem to adversely affect the haemostasis.
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| 44. |
- Hermansson, M, et al.
(author)
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Self-diffusion of oil in lubricating greases by NMR
- 1996
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In: J Synthetic Lubrication. - 1557-6841. ; 13, s. 279-288
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Journal article (peer-reviewed)abstract
- Self diffusion of base oil in six model-lubricating greases and in the base oils themselves were measured by pulsed-field gradient nucleic magnetic resonance (NMR) at 23, 40, 70 and 90 °C. Three greases were based on naphtenic mineral oils and three were based on synthetic polyalphaolefin oils. Soaps of 12-hydroxy stearic acid were used as thickener agents in all six greases. The ratio between the diffusion coefficient of the base oil in grease and neat base oil in itself were evaluated as function of temperature. We judge this ratio as a direct indicator of the obstruction effect that the thickener has on the oil. The thickener showed a stronger obstruction effect on the base oil in the synthetic grease than in the mineral based. This is related to the soap content, which for these greases, is roughly twice as high in the synthetic greases as in the mineral ones. The obstruction effect is constant in the temperature interval 40-90°C for mineral- as well as synthetic based greases. We judge NMR to be a promising method for investigation of how the gellant affects the diffusion coefficient of oil in a lubricating grease.
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| 49. |
- Jansson, E, et al.
(author)
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On the acoustical function of the violin
- 1997
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In: 25. Congresso nazionale : Perugia, 21-23 maggio 1997 : atti / Associazione italiana di acustica.
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Conference paper (peer-reviewed)
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