| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Fodarella, Cristina, et al.
(författare)
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The importance of detailed context reinstatement for the production of identifiable composite faces from memory
- 2021
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Ingår i: Visual cognition (Print). - : Taylor & Francis. - 1350-6285 .- 1464-0716. ; 29:3, s. 180-200
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Tidskriftsartikel (refereegranskat)abstract
- Memory is facilitated by reflecting upon, or revisiting, the environment in which information was encoded. We investigated these “context reinstatement” (CR) techniques to improve the effectiveness of facial composites – visual likenesses of a perpetrator’s face constructed by eyewitnesses. Participant-constructors viewed a face and, after a one-day-delay, revisited (Physical CR) or recalled the environmental context (Mental/Detailed CR) before recalling the face and constructing an EvoFIT or a PRO-fit composite. Detailed CR increased correct naming of ensuing composites, but only when participant-constructors suitably encoded the environment. Detailed CR was also effective when combined with another interviewing technique (Holistic-Cognitive Interview), with focus on a target’s character; it was no more effective prompting constructors to engage in greater environmental recall. Analyses indicate that the Detailed CR advantage was mediated by an increase in face recall. Results are applicable by forensic practitioners to aid eyewitness memory, thereby potentially increasing suspect identification and subsequent arrest rates.
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| 3. |
- Pilla, Rachel M., et al.
(författare)
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Deeper waters are changing less consistently than surface waters in a global analysis of 102 lakes
- 2020
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 10:1
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Tidskriftsartikel (refereegranskat)abstract
- Globally, lake surface water temperatures have warmed rapidly relative to air temperatures, but changes in deepwater temperatures and vertical thermal structure are still largely unknown. We have compiled the most comprehensive data set to date of long-term (1970–2009) summertime vertical temperature profiles in lakes across the world to examine trends and drivers of whole-lake vertical thermal structure. We found significant increases in surface water temperatures across lakes at an average rate of + 0.37 °C decade−1, comparable to changes reported previously for other lakes, and similarly consistent trends of increasing water column stability (+ 0.08 kg m−3 decade−1). In contrast, however, deepwater temperature trends showed little change on average (+ 0.06 °C decade−1), but had high variability across lakes, with trends in individual lakes ranging from − 0.68 °C decade−1 to + 0.65 °C decade−1. The variability in deepwater temperature trends was not explained by trends in either surface water temperatures or thermal stability within lakes, and only 8.4% was explained by lake thermal region or local lake characteristics in a random forest analysis. These findings suggest that external drivers beyond our tested lake characteristics are important in explaining long-term trends in thermal structure, such as local to regional climate patterns or additional external anthropogenic influences.
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