| 1. |
- Klionsky, Daniel J., et al.
(author)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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In: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Research review (peer-reviewed)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 2. |
- Nag, Somnath, et al.
(author)
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Collective and noncollective states in (120)Te
- 2012
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In: Physical Review C (Nuclear Physics). - 0556-2813. ; 85:1
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Journal article (peer-reviewed)abstract
- High-spin states in (120)Te were populated in the reaction (80)Se((48)Ca, alpha 4n)(120)Te at a beam energy of 207 MeV and gamma-ray coincidences were measured using the Gammasphere spectrometer. The previously known level scheme is extended to higher spin and new interband transitions and side-feeding branches are established. Five highly deformed rotational bands, extending up to almost I = 50, are observed for the first time. The bands are compared with similar structures found recently in neighboring nuclei. The experimental results are interpreted within the framework of the cranked Nilsson-Strutinsky model. Configuration assignments to several terminating states and to the high-spin bands are discussed.
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| 3. |
- Nag, Somnath, et al.
(author)
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Revised level structure of Te-120
- 2014
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In: Physical Review C (Nuclear Physics). - 0556-2813. ; 90:3
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Journal article (peer-reviewed)abstract
- The level scheme of the nucleus Te-120, populated in the reaction Se-80(Ca-48, alpha 4n), was reinvestigated using gamma-ray coincidence data measured with the Gammasphere spectrometer. Previously, five high-spin rotational bands were discovered in this nucleus. The present reinvestigation revealed that the decay of band b1 is more complex than suggested in the earlier work and that it cannot be uniquely determined. Furthermore, a number of new transitions are added to the level scheme. The implications for the spin assignments and excitation energies of the five bands and for comparisons with cranked Nilsson-Strutinsky calculations are discussed.
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| 4. |
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| 5. |
- Singh, Purnima, et al.
(author)
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Core excitations beyond maximally aligned configurations in I-123
- 2012
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In: Physical Review C (Nuclear Physics). - 0556-2813. ; 85:3
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Journal article (peer-reviewed)abstract
- High-spin states in I-123 have been populated in the Se-80(Ca-48, p4n) I-123 reaction at 207 MeV and gamma-ray coincidence events have been recorded with the Gammasphere spectrometer. The level scheme of I-123 has been extended up to spin I = 63/2. The nucleus undergoes a shape transition from moderately deformed states with collective rotation at low spins to noncollective oblate configurations at higher spins. Maximally aligned terminating states involving all nine particles outside the Sn-114 core and states with one particle antialigned are identified. A large number of weak transitions feed the terminating states. Cranked Nilsson-Strutinsky calculations have been performed to determine possible configurations for the observed energy levels.
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| 6. |
- Singh, Purnima, et al.
(author)
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High-spin rotational bands in I-123
- 2012
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In: Physical Review C (Nuclear Physics). - 0556-2813. ; 86:6
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Journal article (peer-reviewed)abstract
- High-spin states in I-123 were populated in the reaction Se-80(Ca-48,p4n)I-123 at a beam energy of 207 MeV and gamma-ray coincidence events were measured using the Gammasphere spectrometer. Three weakly populated, high-spin rotational bands have been discovered with characteristics similar to those of the long collective bands recently observed in other nuclei of this mass region. Configuration assignments are proposed based on calculations within the framework of the cranked Nilsson-Strutinsky approach. DOI: 10.1103/PhysRevC.86.067305
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| 7. |
- Cederwall, Bo, et al.
(author)
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Evidence for a spin-aligned neutron-proton paired phase from the level structure of 92Pd
- 2011
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In: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 469:7328, s. 68-71
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Journal article (peer-reviewed)abstract
- Shell structure and magic numbers in atomic nuclei were generally explained by pioneering work(1) that introduced a strong spin-orbit interaction to the nuclear shell model potential. However, knowledge of nuclear forces and the mechanisms governing the structure of nuclei, in particular far from stability, is still incomplete. In nuclei with equal neutron and proton numbers (N = Z), enhanced correlations arise between neutrons and protons (two distinct types of fermions) that occupy orbitals with the same quantum numbers. Such correlations have been predicted to favour an unusual type of nuclear superfluidity, termed isoscalar neutron-proton pairing(2-6), in addition to normal isovector pairing. Despite many experimental efforts, these predictions have not been confirmed. Here we report the experimental observation of excited states in the N = Z = 46 nucleus Pd-92. Gamma rays emitted following the Ni-58(Ar-36,2n)Pd-92 fusion-evaporation reaction were identified using a combination of state-of-the-art high-resolution c-ray, charged-particle and neutron detector systems. Our results reveal evidence for a spin-aligned, isoscalar neutron-proton coupling scheme, different from the previous prediction(2-6). We suggest that this coupling scheme replaces normal superfluidity (characterized by seniority coupling(7,8)) in the ground and low-lying excited states of the heaviest N = Z nuclei. Such strong, isoscalar neutron-proton correlations would have a considerable impact on the nuclear level structure and possibly influence the dynamics of rapid proton capture in stellar nucleosynthesis.
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| 8. |
- Ghazi Moradi, Farnaz, 1976-, et al.
(author)
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Character of particle-hole excitations in Ru-94 deduced from gamma-ray angular correlation and linear polarization measurements
- 2014
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In: Physical Review C. Nuclear Physics. - 0556-2813 .- 1089-490X. ; 89:1, s. 014301-
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Journal article (peer-reviewed)abstract
- Linear polarization and angular correlations of gamma-rays depopulating excited states in the neutron-deficient nucleus Ru-94(44)50 have been measured, enabling firm spin-parity assignments for several excited states in this nucleus. The deduced multipolarities of strong transitions in the yrast structure were found to be mostly of stretched M1, E1, and E2 types and, in most cases, in agreement with previous tentative assignments. The deduced multipolarity of the 1869 keV and the connecting 257 and 1641 keV transitions indicates that the state at 6358 keV excitation energy has spin parity 12(1)(-) rather than 12(3)(+) as proposed in previous works. The presence of a 12(1)(-) state is interpreted within the framework of large-scale shell-model calculations as a pure proton-hole state dominated by the pi(p(1/2)(-1)circle times g(9/2)(-5)) and pi(p(3/2)(-1) g(9/2)(-5)) configurations. A new positive-parity state is observed at 6103 keV and is tentatively assigned as 12(2)(+). The 14(1)(-) state proposed earlier is reassigned as 13(4)(-) and is interpreted as being dominated by neutron particle-hole core excitations. The strengths of several E1 transitions have been measured and are found to provide a signature of core-excited configurations.
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| 9. |
- Ghazi Moradi, Farnaz, et al.
(author)
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Spectroscopy of the neutron-deficient N=50 nucleus Rh-95
- 2014
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In: Physical Review C. Nuclear Physics. - 0556-2813 .- 1089-490X. ; 89:4, s. 044310-
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Journal article (peer-reviewed)abstract
- The neutron-deficient semimagic (neutron number N = 50) Rh-95 nucleus has been produced at high spins using the projectile-target system Ca-40 + Ni-58 at 125 MeV beam energy. The gamma-decays of levels populated by the 3p fusion evaporation reaction channel were studied using gamma-gamma coincidences, and 20 new gamma-ray transitions involving 15 new positive-and negative-parity states were observed. Spin and parity for many of the excited states were firmly deduced for the first time using the combined directional angular correlation and direction-polarization techniques. The observed structures are discussed within the framework of large-scale shell model calculations. E1 transition strengths were deduced and used together with the results of the shell model calculations to study the contribution of different particle-hole configurations, in particular for analyzing contributions from core-excited configurations.
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| 10. |
- Padovani, M., et al.
(author)
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Adaptable radiative transfer innovations for submillimetre telescopes (ARTIST) Dust polarisation module (DustPol)
- 2012
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In: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 543, s. Article Number: A16 -
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Journal article (peer-reviewed)abstract
- We present a new publicly available tool (DustPol) aimed to model the polarised thermal dust emission. The module DustPol, which is publicly available, is part of the ARTIST (Adaptable Radiative Transfer Innovations for Submillimetre Telescopes) package, which also offers tools for modelling the polarisation of line emission together with a model library and a Python-based user interface. DustPol can easily manage analytical as well as pre-gridded models to generate synthetic maps of the Stokes I, Q, and U parameters. These maps are stored in FITS format which is straightforwardly read by the data reduction software used, e. g., by the Atacama Large Millimeter Array (ALMA). This turns DustPol into a powerful engine for the prediction of the expected polarisation features of a source observed with ALMA or the Planck satellite as well as for the interpretation of existing submillimetre observations obtained with other telescopes. DustPol allows the parameterisation of the maximum degree of polarisation and we find that, in a prestellar core, if there is depolarisation, this effect should happen at densities of 10(6) cm(-3) or larger. We compare a model generated by DustPol with the observational polarisation data of the low-mass Class 0 object NGC 1333 IRAS 4A, finding that the total and the polarised emission are consistent.
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| 11. |
- Zheng, Y., et al.
(author)
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gamma-ray linear polarization measurements and (g(9/2))(-3) neutron alignment in Ru-91
- 2013
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In: Physical Review C. Nuclear Physics. - : American Physical Society (APS). - 0556-2813 .- 1089-490X. ; 87:4, s. 044328-
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Journal article (peer-reviewed)abstract
- Linear polarization measurements have been performed for gamma rays in Ru-91 produced with the Ni-58(Ar-36,2p1n gamma)Ru-91 reaction at a beam energy of 111 MeV. The EXOGAM Ge clover array has been used to measure the gamma-gamma coincidences, gamma-ray linear polarization, and gamma-ray angular distributions. The polarization sensitivity of the EXOGAM clover detectors acting as Compton polarimeters has been determined in the energy range 0.3-1.3 MeV. Several transitions have been observed for the first time. Measurements of linear polarization and angular distribution have led to the firm assignments of spin differences and parity of high-spin states in Ru-91. More specifically, calculations using a semiempirical shell model were performed to understand the structures of the first and second (21/2(+)) and (17/2(+)) levels. The results are in good agreement with the experimental data, supporting the interpretation of the nonyrast (21/2(+)) and (17/2(+)) states in terms of the J(max) and J(max) - 2 members of the seniority-three nu(g(9/2))(-3) multiplet.
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