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Process development...
Process development of oxygen-demanding reactions utilizing a simple design with parallel glass tube reactors - Evaluated using Gluconobacter oxydans (DSM 24525)
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- Grey, Carl (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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- Viloria-Cols, Maria (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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Jungvid, Hans (författare)
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- Adlercreutz, Patrick (författare)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
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(creator_code:org_t)
- 2012-11-08
- 2012
- Engelska.
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Ingår i: Biocatalysis and Biotransformation. - : Informa UK Limited. - 1024-2422 .- 1029-2446. ; 30:5-6, s. 441-445
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- To investigate the possibility of using simple glass tubes as reactors for oxygen-demanding reactions, a setup was assembled to study the initial rate of conversion of glycerol to dihydroxyacetone (DHA) using Gluconobacter oxydans. Several parallel 10 mL glass tubes were incubated in a temperature-controlled shaker. The concentration of DHA was determined using a fast spectrophotometric HPLC-based method that could process 3 samples/min. It was shown that the obtained results were reproducible and the reaction rates remained constant throughout the reaction. Further, the system reached a high volumetric activity of 15.48 g DHA L-1 h(-1) consuming 86 mmol L-1 h(-1) oxygen before the system became mass-transfer limited, indicating a high diffusion of oxygen. It was concluded that the reactor system is well suited for process development where the requirement for oxygen is high and that the assay developed can be used to determine the initial rate of DHA production.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Nyckelord
- bioreactor
- oxygen
- activity assay
- glycerol
- dihydroxyacetone
- Gluconobacter oxydans
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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