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- Wroblewski, R, et al.
(författare)
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X-ray microanalysis of in situ and isolated pancreatic islets
- 1998
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Ingår i: Pancreas. - 0885-3177 .- 1536-4828. ; 16:2, s. 134-140
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Tidskriftsartikel (refereegranskat)abstract
- The effect of stimulation of insulin secretion in pancreatic beta cells on the elemental composition ofthese cells was investigated by x-ray microanalysis. In vitro experiments on isolated islets ofLangerhans from ob/ob mice were compared to in situ experiments. The only significant difference inthe elemental composition of beta cells from ob/ob mice versus their lean counterparts is a lower Ca concentration in the ob/ob animals. The nucleus of the beta cells has a higher concentration of P, K, and Na than the cytoplasm, which has a higher concentration of S and Cl. No polarized ion distributionin the cytoplasm of the beta cells was observed. Isolated beta cells show a higher concentration of Na and Cl and a lower concentration of K than their in situ counterparts. Stimulation of insulin secretion with glucose both in situ and in vitro showed only very small effects on the elemental composition of the beta cells: a tendency to a decreased P content was noted. In vitro experiments using stimulation with high extracellular K+ showed, in addition, a small increase in the intracellular K concentration. Inconclusion, while the elemental content of beta cells in vitro differs from that in situ, the response to glucose stimulation appears to be similar in both systems.
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