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Search: WFRF:(Kern G) > (2000-2004)

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  • Sarrou, J, et al. (author)
  • Nitric oxide-induced formation of the S-2 state in the oxygen-evolving complex of photosystem II from Synechococcus elongatus
  • 2003
  • In: Biochemistry. - : AMER CHEMICAL SOC. - 0006-2960 .- 1520-4995. ; 42:4, s. 1016-1023
  • Journal article (peer-reviewed)abstract
    • In spinach photosystem II (PSII) membranes, the tetranuclear manganese cluster of the oxygen-evolving complex (OEC) can be reduced by incubation with nitric oxide at -30 degreesC to a state which is characterized by an Mn-2(II, III) EPR multiline signal [Sarrou, J., Ioannidis, N., Deligiannakis, Y., and Petrouleas, V. (1998) Biochemistry 37, 3581-3587]. This state was recently assigned to the S-2 state of the OEC [Schansker, G., Goussias, C., Petrouleas, V., and Rutherford, A. W. (2002) Biochemistry 41, 3057-3064]. On the basis of EPR spectroscopy and flash-induced oxygen evolution patterns, we show that a similar reduction process takes place in PSII samples of the thermophilic cyanobacterium Synechococcus elongatus at both -30 and 0 degreesC. An EPR multiline signal, very similar but not identical to that of the S-2 state in spinach, was obtained with monomeric and dimeric PSII core complexes from S. elongatus only after incubation at -30 degreesC. The assignment of this EPR multiline signal to the S-2 state is corroborated by measurements of flash-induced oxygen evolution patterns and detailed fits using extended Kok models. The small reproducible shifts of several low-field peak positions of the S-2 EPR multiline signal in S. elongatus compared to spinach suggest that slight differences in the coordination geometry and/or the ligands of the manganese cluster exist between thermophilic cyanobacteria and higher plants.
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