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IAP-Based Cell Sort...
IAP-Based Cell Sorting Results in Homogeneous Transplantable Dopaminergic Precursor Cells Derived from Human Pluripotent Stem Cells
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- Lehnen, Daniela (author)
- Miltenyi Biotec GmbH
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- Barral, Serena (author)
- Aix-Marseille University,University College London
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- Cardoso, Tiago (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups
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- Grealish, Shane (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups
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- Heuer, Andreas (author)
- Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine
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- Smiyakin, Andrej (author)
- Miltenyi Biotec GmbH
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- Kirkeby, Agnete (author)
- Lund University,Lunds universitet,Human neural utvecklingsbiologi,Forskargrupper vid Lunds universitet,Human Neural Developmental Biology,Lund University Research Groups
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- Kollet, Jutta (author)
- Miltenyi Biotec GmbH
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- Cremer, Harold (author)
- Aix-Marseille University
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- Parmar, Malin (author)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups
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- Bosio, Andreas (author)
- Miltenyi Biotec GmbH
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- Knöbel, Sebastian (author)
- Miltenyi Biotec GmbH
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(creator_code:org_t)
- Elsevier BV, 2017
- 2017
- English.
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In: Stem Cell Reports. - : Elsevier BV. - 2213-6711. ; 9:4, s. 1207-1220
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http://www.cell.com/...
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Abstract
Subject headings
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- Human pluripotent stem cell (hPSC)-derived mesencephalic dopaminergic (mesDA) neurons can relieve motor deficits in animal models of Parkinson's disease (PD). Clinical translation of differentiation protocols requires standardization of production procedures, and surface-marker-based cell sorting is considered instrumental for reproducible generation of defined cell products. Here, we demonstrate that integrin-associated protein (IAP) is a cell surface marker suitable for enrichment of hPSC-derived mesDA progenitor cells. Immunomagnetically sorted IAP+ mesDA progenitors showed increased expression of ventral midbrain floor plate markers, lacked expression of pluripotency markers, and differentiated into mature dopaminergic (DA) neurons in vitro. Intrastriatal transplantation of IAP+ cells sorted at day 16 of differentiation in a rat model of PD resulted in functional recovery. Grafts from sorted IAP+ mesDA progenitors were more homogeneous in size and DA neuron density. Thus, we suggest IAP-based sorting for reproducible prospective enrichment of mesDA progenitor cells in clinical cell replacement strategies. In this article, Knöbel and colleagues identified IAP as a cell surface marker for mesDA progenitor cells. Immunomagnetic sorting for IAP+ led to reproducible and homogeneous cell compositions. Intrastriatal transplantation of sorted cells at day 16 of differentiation in a PD rat model resulted in functional recovery, and grafts were more homogeneous in size and DA neuron density than unsorted cells.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- CD47
- Cell replacement therapy
- Dopaminergic
- Floor plate
- IAP
- Immunomagnetic sorting (MACS)
- MesDA progenitor cells
- Midbrain
- Parkinson's disease
- PD
- Regenerative medicine
Publication and Content Type
- art (subject category)
- ref (subject category)
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To the university's database
- By the author/editor
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Lehnen, Daniela
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Barral, Serena
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Cardoso, Tiago
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Grealish, Shane
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Heuer, Andreas
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Smiyakin, Andrej
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show more...
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Kirkeby, Agnete
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Kollet, Jutta
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Cremer, Harold
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Parmar, Malin
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Bosio, Andreas
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Knöbel, Sebastia ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
- Articles in the publication
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Stem Cell Report ...
- By the university
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Lund University