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- Gladyshev, VN, et al.
(författare)
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Selenoprotein Gene Nomenclature
- 2016
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Ingår i: The Journal of biological chemistry. - 1083-351X. ; 291:46, s. 24036-24040
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Tidskriftsartikel (refereegranskat)
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| 3. |
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| 4. |
- Pietrzak, Witold, et al.
(författare)
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Ethanol, feed components and fungal biomass production from field bean (Vicia faba var. equina) seeds in an integrated process
- 2016
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Ingår i: Bioresource Technology. - 0960-8524 .- 1873-2976. ; 216, s. 69-76
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Tidskriftsartikel (refereegranskat)abstract
- The use of field beans, a non-food leguminous crop, was studied for ethanol, feed components and fungal biomass production. The seeds were hydrolyzed using enzymes or with combination of acid (H3PO4) and alkaline (Ca(OH)2) pretreatment and enzymatic hydrolysis. Fermentation by Saccharomyces cerevisiae, with or without removal of suspended solids, yielded 38.3-42.5gL(-1) ethanol (71.3-79.2% efficiency). The filtration residues contained ca. 247-326gkg(-1) crude protein, 10.6-15.5% acid detergent fiber and 19.9-29.1% neutral detergent fiber. They were enriched in phenolics (by up to 93.4%) and depleted in condensed tannin (by up to 59.3%) in comparison to the raw material. The thin stillages were used for cultivation of edible fungus Neurospora intermedia which produced 8.5-15.9gL(-1) ethanol and 4.8-16.2gL(-1) biomass containing over 62% protein. The mass balances showed that fermentation of unfiltered mashes was more efficient yielding up to 195.9gkg(-1) ethanol and 84.4% of protein recovery.[on SciFinder (R)]
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| 5. |
- Schmidtlein, Charles R., et al.
(författare)
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Initial performance studies of a wearable brain positron emission tomography camera based on autonomous thin-film digital Geiger avalanche photodiode arrays
- 2017
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Ingår i: Journal of Medical Imaging. - : SPIE - International Society for Optical Engineering. - 2329-4302 .- 2329-4310. ; 4:1
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Tidskriftsartikel (refereegranskat)abstract
- Using analytical and Monte Carlo modeling, we explored performance of a lightweight wearable helmet-shaped brain positron emission tomography (PET), or BET camera, based on thin-film digital Geiger avalanche photodiode arrays with Lutetium-yttrium oxyorthosilicate (LYSO) or LaBr3 scintillators for imaging in vivo human brain function of freely moving and acting subjects. We investigated a spherical cap BET and cylindrical brain PET (CYL) geometries with 250-mm diameter. We also considered a clinical whole-body (WB) LYSO PET/CT scanner. The simulated energy resolutions were 10.8% (LYSO) and 3.3% (LaBr3), and the coincidence window was set at 2 ns. The brain was simulated as a water sphere of uniform F-18 activity with a radius of 100 mm. We found that BET achieved >40% better noise equivalent count (NEC) performance relative to the CYL and >800% than WB. For 10-mm-thick LaBr3 equivalent mass systems, LYSO (7-mm thick) had similar to 40% higher NEC than LaBr3. We found that 1 x 1 x 3 mm scintillator crystals achieved similar to 1.1 mm full-width-half-maximum spatial resolution without parallax errors. Additionally, our simulations showed that LYSO generally outperformed LaBr3 for NEC unless the timing resolution for LaBr3 was considerably smaller than that presently used for LYSO, i.e., well below 300 ps.
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| 6. |
- Wang, Bo, et al.
(författare)
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Transient production of artemisinin in Nicotiana benthamiana is boosted by a specific lipid transfer protein from A. annua
- 2016
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Ingår i: Metabolic engineering. - : Elsevier BV. - 1096-7176 .- 1096-7184. ; 38, s. 159-169
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Tidskriftsartikel (refereegranskat)abstract
- Our lack of full understanding of transport and sequestration of the heterologous products currently limit metabolic engineering in plants for the production of high value terpenes. For instance, although all genes of the artemisinin/arteannuin B (AN/AB) biosynthesis pathway (AN-PW) from Artemisia annua have been identified, ectopic expression of these genes in Nicotiana benthamiana yielded mostly glycosylated pathway intermediates and only very little free (dihydro)artemisinic acid [(DH)AA]. Here we demonstrate that Lipid Transfer Protein 3 (AaLTP3) and the transporter Pleiotropic Drug Resistance 2 (AaPDR2) from A. annua enhance accumulation of (DH)AA in the apoplast of N. benthamiana leaves. Analysis of apoplast and cell content and apoplast exclusion assays show that AaLTP3 and AaPDR2 prevent reflux of (DH)AA from the apoplast back into the cells and enhances overall flux through the pathway. Moreover, AaLTP3 is stabilized in the presence of AN-PW activity and co-expression of AN-PW+AaLTP3+AaPDR2 genes yielded AN and AB in necrotic N. benthamiana leaves at 13 days post-agroinfiltration. This newly discovered function of LTPs opens up new possibilities for the engineering of biosynthesis pathways of high value terpenes in heterologous expression systems.
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