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1.
  • Lozano, Rafael, et al. (author)
  • Measuring progress from 1990 to 2017 and projecting attainment to 2030 of the health-related Sustainable Development Goals for 195 countries and territories: a systematic analysis for the Global Burden of Disease Study 2017
  • 2018
  • In: The Lancet. - : Elsevier. - 1474-547X .- 0140-6736. ; 392:10159, s. 2091-2138
  • Journal article (peer-reviewed)abstract
    • Background: Efforts to establish the 2015 baseline and monitor early implementation of the UN Sustainable Development Goals (SDGs) highlight both great potential for and threats to improving health by 2030. To fully deliver on the SDG aim of “leaving no one behind”, it is increasingly important to examine the health-related SDGs beyond national-level estimates. As part of the Global Burden of Diseases, Injuries, and Risk Factors Study 2017 (GBD 2017), we measured progress on 41 of 52 health-related SDG indicators and estimated the health-related SDG index for 195 countries and territories for the period 1990–2017, projected indicators to 2030, and analysed global attainment. Methods: We measured progress on 41 health-related SDG indicators from 1990 to 2017, an increase of four indicators since GBD 2016 (new indicators were health worker density, sexual violence by non-intimate partners, population census status, and prevalence of physical and sexual violence [reported separately]). We also improved the measurement of several previously reported indicators. We constructed national-level estimates and, for a subset of health-related SDGs, examined indicator-level differences by sex and Socio-demographic Index (SDI) quintile. We also did subnational assessments of performance for selected countries. To construct the health-related SDG index, we transformed the value for each indicator on a scale of 0–100, with 0 as the 2·5th percentile and 100 as the 97·5th percentile of 1000 draws calculated from 1990 to 2030, and took the geometric mean of the scaled indicators by target. To generate projections through 2030, we used a forecasting framework that drew estimates from the broader GBD study and used weighted averages of indicator-specific and country-specific annualised rates of change from 1990 to 2017 to inform future estimates. We assessed attainment of indicators with defined targets in two ways: first, using mean values projected for 2030, and then using the probability of attainment in 2030 calculated from 1000 draws. We also did a global attainment analysis of the feasibility of attaining SDG targets on the basis of past trends. Using 2015 global averages of indicators with defined SDG targets, we calculated the global annualised rates of change required from 2015 to 2030 to meet these targets, and then identified in what percentiles the required global annualised rates of change fell in the distribution of country-level rates of change from 1990 to 2015. We took the mean of these global percentile values across indicators and applied the past rate of change at this mean global percentile to all health-related SDG indicators, irrespective of target definition, to estimate the equivalent 2030 global average value and percentage change from 2015 to 2030 for each indicator. Findings: The global median health-related SDG index in 2017 was 59·4 (IQR 35·4–67·3), ranging from a low of 11·6 (95% uncertainty interval 9·6–14·0) to a high of 84·9 (83·1–86·7). SDG index values in countries assessed at the subnational level varied substantially, particularly in China and India, although scores in Japan and the UK were more homogeneous. Indicators also varied by SDI quintile and sex, with males having worse outcomes than females for non-communicable disease (NCD) mortality, alcohol use, and smoking, among others. Most countries were projected to have a higher health-related SDG index in 2030 than in 2017, while country-level probabilities of attainment by 2030 varied widely by indicator. Under-5 mortality, neonatal mortality, maternal mortality ratio, and malaria indicators had the most countries with at least 95% probability of target attainment. Other indicators, including NCD mortality and suicide mortality, had no countries projected to meet corresponding SDG targets on the basis of projected mean values for 2030 but showed some probability of attainment by 2030. For some indicators, including child malnutrition, several infectious diseases, and most violence measures, the annualised rates of change required to meet SDG targets far exceeded the pace of progress achieved by any country in the recent past. We found that applying the mean global annualised rate of change to indicators without defined targets would equate to about 19% and 22% reductions in global smoking and alcohol consumption, respectively; a 47% decline in adolescent birth rates; and a more than 85% increase in health worker density per 1000 population by 2030. Interpretation: The GBD study offers a unique, robust platform for monitoring the health-related SDGs across demographic and geographic dimensions. Our findings underscore the importance of increased collection and analysis of disaggregated data and highlight where more deliberate design or targeting of interventions could accelerate progress in attaining the SDGs. Current projections show that many health-related SDG indicators, NCDs, NCD-related risks, and violence-related indicators will require a concerted shift away from what might have driven past gains—curative interventions in the case of NCDs—towards multisectoral, prevention-oriented policy action and investments to achieve SDG aims. Notably, several targets, if they are to be met by 2030, demand a pace of progress that no country has achieved in the recent past. The future is fundamentally uncertain, and no model can fully predict what breakthroughs or events might alter the course of the SDGs. What is clear is that our actions—or inaction—today will ultimately dictate how close the world, collectively, can get to leaving no one behind by 2030.
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4.
  • Kumar, Manoj, et al. (author)
  • Arsenic distribution and mobilization : A case study of three districts of uttar pradesh and bihar (india)
  • 2015
  • In: Safe and Sustainable Use of Arsenic-Contaminated Aquifers in the Gangetic Plain. - Cham : Springer Publishing Company. - 9783319161242 - 9783319161235 ; , s. 111-123
  • Book chapter (other academic/artistic)abstract
    • Tectonic evolution of Himalayas is related to high erosional potential and substantial sediment transport. Fluvial deposition of clastic material in the Middle Gangetic plain (MGP) is mainly governed by crustal deformation and climatic condition of Himalayas (Singh M, Singh IB, Müller G, Geomorphology 86:144-175, 2007). Seven large Asian rivers-Ganga, Indus, Brahmaputra, Yangtze, Huang He or Yellow River, Salween and Mekong-are fed by Himalayan glaciers which are supplying ~30 % of the global sediments to the ocean (Milliman JD, Meade RH, J Geol 9:1-19, 1983; Singh VB, Ramanathan AL, Pottakkal JG, Kumar M, J Asian Earth Sci 79:224-234, 2014, 2005). High flux of sediment transported from different terrain of Himalayas is product of geologically young rock formation (Singh VB, Ramanathan AL, Pottakkal JG, Kumar M, J Asian Earth Sci 79:224-234, 2014). It provides an opportunity to study the fluvial system and post-depositional changes in sediment water interaction depending on the degree of mobility of element under the altered environmental conditions. Arsenic (As) contamination of groundwater is a global problem. Understanding of As mobilization from sediments to As-contaminated aquifers is important for water quality management in areas of MGP of India.
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5.
  • Mohan, Gowtham, et al. (author)
  • Experimental investigation of a novel solar thermal polygeneration plant in United Arab Emirates
  • 2016
  • In: Renewable energy. - : Elsevier BV. - 0960-1481 .- 1879-0682. ; 91, s. 361-373
  • Journal article (peer-reviewed)abstract
    • The demands for space air conditioning and clean drinking water are relatively high in Middle East North African (MENA) countries. A sustainable and innovative approach to meet these demands along with the production of domestic hot water is experimentally investigated in this paper. A novel solar thermal poly-generation (STP) pilot plant is designed and developed for production of chilled water for air conditioning using absorption chiller, clean drinking water with membrane distillation units and domestic hot water by heat recovery. The STP system is developed with a flexibility to operate in four different modes: (i) solar cooling mode (ii) cogeneration of drinking water and domestic hot water (iii) cogeneration of cooling and desalination (iv) trigeneration. Operational flexibility allows consumers to utilize the available energy based on seasonal requirements. Performance of STP system is analyzed during summer months in RAKRIC research facility. Energy flows in STP pilot plant during peak load operations are analyzed for all four modes. STP system with trigeneration mode utilizes 23% more useful energy compared to solar cooling mode, which improves overall efficiency of the plant. Economic benefits of STP with trigeneration mode are evaluated with fuel cost inflation rate of 10%. STP plant has potential payback period of 9.08 years and net cumulative savings of $454,000 based on economic evaluation.
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6.
  • Pandey, Kavita, et al. (author)
  • Improving electron transport in the hybrid perovskite solar cells using CaMnO3-based buffer layer
  • 2018
  • In: Nano Energy. - : ELSEVIER SCIENCE BV. - 2211-2855 .- 2211-3282. ; 45, s. 287-297
  • Journal article (peer-reviewed)abstract
    • In the present article, the detailed analyses of interface properties and device performance of inorganic perovskite CaMnO3-based buffer layer hybrid perovskite solar cell have been undertaken. Analyses are based on ab initio simulations and macroscopic modelling. A thorough study of electronic and optical properties and interface charge dynamics revealed that CaMnO3 presents a better candidate for the electron transport material in thin film hole transporting material free hybrid perovskite solar cells with the planar architecture than the most common anatase TiO2. This result is founded on the more appropriate band gap and better band alignment with the hybrid perovskite, leading to the faster charge carrier mobility, improved charge transfer and reduced exciton recombination. The results from theoretical simulations are justified by the solar cell model, which explored the basic cell characteristics and parameters: open circuit voltage, short circuit current, fill factor and efficiency, as the functions of cell performance factors, like defect density, diffusion length, absorber layer thickness and band offset. Our model suggests an unoptimized device with a photo-conversion efficiency of almost 10% for the low defect concentrations under 10(15). With efficiency in the upper range for HTM free perovskite solar cells, we propose that the CaMnO3-based solar cell poses as an improvement upon the up to now most frequently used ones and provides important step toward their commercialisation.
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  • Sundell, David, et al. (author)
  • AspWood : High-Spatial-Resolution Transcriptome Profiles Reveal Uncharacterized Modularity of Wood Formation in Populus tremula
  • 2017
  • In: The Plant Cell. - : Oxford University Press (OUP). - 1040-4651 .- 1532-298X. ; 29:7, s. 1585-1604
  • Journal article (peer-reviewed)abstract
    • Trees represent the largest terrestrial carbon sink and a renewable source of ligno-cellulose. There is significant scope for yield and quality improvement in these largely undomesticated species, and efforts to engineer elite varieties will benefit from improved understanding of the transcriptional network underlying cambial growth and wood formation. We generated high-spatial-resolution RNA sequencing data spanning the secondary phloem, vascular cambium, and wood-forming tissues of Populus tremula. The transcriptome comprised 28,294 expressed, annotated genes, 78 novel protein-coding genes, and 567 putative long intergenic noncoding RNAs. Most paralogs originating from the Salicaceae whole-genome duplication had diverged expression, with the exception of those highly expressed during secondary cell wall deposition. Coexpression network analyses revealed that regulation of the transcriptome underlying cambial growth and wood formation comprises numerous modules forming a continuum of active processes across the tissues. A comparative analysis revealed that a majority of these modules are conserved in Picea abies. The high spatial resolution of our data enabled identification of novel roles for characterized genes involved in xylan and cellulose biosynthesis, regulators of xylem vessel and fiber differentiation and lignification. An associated web resource (AspWood, http://aspwood.popgenie.org) provides interactive tools for exploring the expression profiles and coexpression network.
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8.
  • Sundell, David, et al. (author)
  • High-spatial-resolution transcriptome profiling reveals uncharacterized regulatory complexity underlying cambial growth and wood formation in Populus tremula
  • 2016
  • Other publication (other academic/artistic)abstract
    • Trees represent the largest terrestrial carbon sink and a renewable source of ligno-cellulose. There is significant scope for yield and quality improvement in these largely undomesticated species, however, efforts to engineer new, elite varieties are constrained by the lack of a comprehensive understanding of the transcriptional network underlying cambial growth and wood formation. We generated RNA Sequencing transcriptome data for four mature, wild-growing aspens (Populus tremula) from high-spatial-resolution tangential cryosection series spanning the secondary phloem, vascular cambium, expanding and secondary cell wall forming xylem cells, cell death zone and the previous years annual ring. The transcriptome comprised 28,294 expressed, previously annotated protein-coding genes, 78 novel protein-coding genes and 567 long intergenic non-coding RNAs. Most paralogs originating from the Salicaceae whole genome duplication had diverged expression, with the notable exception of those with high expression during secondary cell wall deposition. We performed co-expression network analysis to identify central transcriptional modules and associated several of these with known biological processes. This revealed previously uncharacterized complexity underlying the regulation of cambial growth and wood formation, with modules forming a continuum of activated processes across the tissues. The high spatial resolution suggested novel roles for known genes involved in xylan and cellulose biosynthesis, regulators of xylem vessel and fiber differentiation and components of lignification. The associated web resource (AspWood, http://aspwood.popgenie.org) integrates the data within a set of interactive tools for exploring the co-expression network of cambial growth and wood formation.
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  • Bandaru, Manoj Kumar, 1987- (author)
  • Zebrafish models for large-scale genetic screens in dyslipidemia and atherosclerosis : Validation and application
  • 2019
  • Doctoral thesis (other academic/artistic)abstract
    • Hundreds of loci have been robustly associated with circulating lipids, atherosclerosis and coronary artery disease; but for most loci the causal genes and mechanisms remain uncharacterized. The overall aim of my thesis is to develop and validate novel in vivo model systems that are suitable for high-throughput, image-based genetic screens in coronary artery disease and related traits, and use these model systems to systematically characterize positional candidate genes.In Study I, I developed an experimental pipeline to validate the suitability of zebrafish larvae as a model system for systematic, large-scale characterization of drugs and genes associated with dyslipidemia and atherosclerosis. Using this pipeline, I showed that five days of overfeeding and cholesterol supplementation have independent pro-atherogenic effects in zebrafish larvae, which could be diminished by concomitant treatment with atorvastatin and ezetimibe. CRISPR-Cas9-induced mutations in orthologues of proof-of-concept genes resulted in higher LDL cholesterol levels (apoea), and more early stage atherosclerosis (apobb.1). Finally, the pipeline helped me to identify putative causal genes for circulating lipids and early-stage atherosclerosis (LPAR2 and GATAD2A).In Study II, I characterized cardiometabolic traits in apoc2 mutant zebrafish larvae and found that, similar to humans, larvae with two non-functional apoc2 alleles have higher whole-body levels of triglycerides and total cholesterol, and more vascular lipid deposition than larvae without mutations in apoc2. Interestingly, apoc2 mutant larvae also had lower glucose levels after adjusting for triglyceride levels, suggesting that therapeutic stimulation of apoc2 to prevent hypertriglyceridemia may result in hyperglycemia. Still, zebrafish larvae with mutations in apoc2 can be a useful model to identify and characterize additional causal genes for triglyceride metabolism.In Study III, I examined the effects of mutations in pcsk9 on atherosclerosis and diabetes-related traits in nearly 5,000 zebrafish larvae. Similar to the loss-of-function mutations in PCSK9 in humans, larvae with mutations in pcsk9 had lower LDLc levels and were protected from early-stage atherosclerosis. Interestingly, mutations in pcsk9 also resulted in fewer pancreatic β-cells in 10 days old larvae, which suggests the higher risk of diabetes in humans with mutations in PCSK9 may result from a direct effect on the beta cell.Based on these large-scale proof-of-concept studies, my thesis confirms that zebrafish larvae can be used for large-scale, systematic genetic screens in dyslipidemia and early-stage atherosclerosis.
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10.
  • Gallardo, Rodrigo, et al. (author)
  • De novo design of a biologically active amyloid
  • 2016
  • In: Science. - : AMER ASSOC ADVANCEMENT SCIENCE. - 0036-8075 .- 1095-9203. ; 354:6313, s. 720-
  • Journal article (peer-reviewed)abstract
    • Most human proteins possess amyloidogenic segments, but only about 30 are associated with amyloid-associated pathologies, and it remains unclear what determines amyloid toxicity. We designed vascin, a synthetic amyloid peptide, based on an amyloidogenic fragment of vascular endothelial growth factor receptor 2 (VEGFR2), a protein that is not associated to amyloidosis. Vascin recapitulates key biophysical and biochemical characteristics of natural amyloids, penetrates cells, and seeds the aggregation of VEGFR2 through direct interaction. We found that amyloid toxicity is observed only in cells that both express VEGFR2 and are dependent on VEGFR2 activity for survival. Thus, amyloid toxicity here appears to be both protein-specific and conditional-determined by VEGFR2 loss of function in a biological context in which target protein function is essential.
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11.
  • Gerber, Lorenz, et al. (author)
  • High-throughput microanalysis of large lignocellulosic sample sets by pyrolysis-gas chromatography/mass spectrometry
  • 2016
  • In: Physiologia Plantarum. - : Wiley. - 0031-9317 .- 1399-3054. ; 156, s. 127-138
  • Journal article (peer-reviewed)abstract
    • High-throughput analytical techniques to assess the chemistry of lignocellulosic plant material are crucial to plant cell-wall research. We have established an analytical platform for this purpose and demonstrated its usefulness with two applications. The system is based on analytical pyrolysis, coupled to gas chromatography/mass spectrometry - a technique particularly suited for analysis of lignocellulose. Automated multivariate-based data-processing methods are used to obtain results within a few hours after analysis, with an experimental batch of 500 analyzed samples. The usefulness of multivariate sample discrimination methods and hierarchical clustering of samples is demonstrated. We have analyzed an Arabidopsis mutant collection consisting of 300 samples representing 31 genotypes. The mutant collection is presented through cluster analysis, based on chemotypic difference, with respect to wild type. Further, we have analyzed 500 thin sections from five biological replicate trees to create a spatial highly resolved profile of the proportions of syringyl-, guaiacyl- and p-hydroxyphenyl lignin across phloem, developing and mature wood in aspen. The combination of biologically easy to interpret information, the low demand of sample amount and the flexibility in sample types amenable to analysis makes this technique a valuable extension to the range of established high-throughput biomaterial analytical platforms.
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  • Hedegård, Lars, 1979-, et al. (author)
  • Contradictions In Reuse-based Fashion Retail - the ReTuna Mall Case
  • 2016
  • In: GLOBAL FASHION CONFERENCE 2016 STOCKHOLM – SWEDEN. - 9789892070537
  • Conference paper (other academic/artistic)abstract
    • PurposeThe purpose of this paper is to describe and analyse the management of a novel commercial fashion retail concept – a shopping mall based on reuse and a local circular fashion supply chain – with the aim of identifying potential strategic issues with the concept.Design/methodology/approachThis is an explorative case study, based on observations and interviews with shop managers, employees and the mall management.FindingsThe reuse concept strongly influences the mall's strategy, and the sourcing process is a key factor. The local reuse-based fashion supply chain follows the typical reuse process, but this study shows that the business logic that underpins the commercial strategy is not in line with the reuse and social enterprise ethoses that the mall ostensibly follows.Research limitations/implicationsThis study illustrates the difficulties inherent in organising a reuse-based mall due to the need to combine a commercial strategy, a local and circular fashion supply chain, and a social enterprise ethic.Practical implicationsThe findings highlight the mall management's responsibility for the sourcing of goods, the need for a closer cooperation between mall management and tenants in a reuse-based mall, and the need for competence in terms of reuse, fashion, and retail in order for the concept to be developed further.Originality/valueReTuna represents a new fashion retail phenomenon – the reuse-based shopping mall – that has not been studied yet.KeywordsFashion retail, textile reuse, clothing reuse, mall management, fashion supply chain, recycling, sustainability, circular supply chain.Article classificationResearch paper
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  • Hedegård, Lars, 1979-, et al. (author)
  • Management of sustainable fashion retail based on reuse : A struggle with multiple logics
  • 2019
  • In: The International Review of Retail, Distribution and Consumer Research. - : Informa UK Limited. - 0959-3969 .- 1466-4402.
  • Journal article (peer-reviewed)abstract
    • In scholarly conversations, reuse is one of the common suggested strategies to render fashion retail sustainable. Previous research has stressed the complexity of fashion reuse and the importance of a well-organized system. The complexity stems from processes that involve many actors as well as products hard to evaluate. Consequently, it is challenging to organize reuse-based fashion retail, and studies are needed to further develop knowledge regarding how to manage such systems. Hence, the purpose of this paper is to highlight the complexity in the management of such an initiative, by identifying and explaining obstacles as well as implications. With institutional logics as a framework, three local logics (shopping mall, reuse, and work integration) are used to analyze the management of a reuse-based mall. Despite the mall’s success in getting sufficient donations and creating publicity, it has struggled to establish itself as viable reuse-based fashion retail. The findings illustrate the complexity created by the interplay of different logics and how the complexity influences both the daily and strategic management of the mall. Further, the outcome of this interplay depends largely on which rationality is enacted by involved actors. The study also extends literature on institutional logics, showing that differences in individual actors’ attention, knowledge, skills, coordination, and material conditions influence how logics are enacted and managed. We suggest that there are inherent managerial contradictions in the sustainable practices in fashion retail. Thus, in scholarly conversations, it is important to discuss what different divergent sustainability dimensions imply when seeking solutions for sustainable retail. In practice, there is a need to acknowledge and balance the presence of multiple logics, making it crucial to have competence in all logics. Also, managers of reuse-based fashion retail must consciously and continuously scrutinize their own strategies and actions to avoid an imbalance between the logics.
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  • Hedegård, Lars, 1979-, et al. (author)
  • MANAGEMENT OF SUSTAINABLE FASHION RETAIL BASED ON REUSE – A STRUGGLE WITH MULTIPLE LOGICS
  • 2019
  • Conference paper (peer-reviewed)abstract
    • Reuse is a strategy to render fashion retail sustainable and an example is the take-back schemes established by international retailers. Managerial aspects are important in a reuse system, but management issues have seldom been studied. Accordingly, empirical investigations of the management of reuse systems are needed. Hence, the purpose of this study is to show the complexity in the management of fashion-retail based on reuse by identifying and explaining obstacles in the process. This is achieved by an analyze of ReTuna, a shopping mall based on reuse, from the perspective of institutional logics. ReTuna opened in 2015 and consists of approximately fourteen stores. The shops at ReTuna sell reused products, but this unconventional sourcing of goods aside, ReTuna aims to be a traditional mall. Most shops are staffed by the owner(s) and in some cases an employee. Garments and textiles that are sold origins from donations that are collected by the mall. The case illustrates the complexity, as it failed in establishing reuse-based fashion retail, despite its success in achieving enough donations and creating publicity. The analysis shows that the goal of re-circulating fashion is hindered by actors not being able to equally integrate the divergent sustainability dimensions in the mall owner’s goals. The obstacles are a result of the actors prioritizing the logics differently at the same time as not being able to fulfill the demands of the logics due to a lack of knowledge, experience and skills, and coordination.
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  • Johri, Atul K., et al. (author)
  • Fungal association and utilization of phosphate by plants : success, limitations, and future prospects
  • 2015
  • In: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 6
  • Research review (peer-reviewed)abstract
    • Phosphorus (P) is a major macronutrient for plant health and development. The available form of P is generally low in the rhizosphere even in fertile soils. A major proportion of applied phosphate (Pi) fertilizers in the soil become fixed into insoluble, unavailable forms, which restricts crop production throughout the world. Roots possess two distinct modes of P uptake from the soil, direct and indirect uptake. The direct uptake of P is facilitated by the plant's own Pi transporters while indirect uptake occurs via mycorrhizal symbiosis, where the host plant obtains P primarily from the fungal partner, while the fungus benefits from plant-derived reduced carbon. So far, only one Pi transporter has been characterized from the mycorrhizal fungus Glomus versiforme. As arbuscular mycorrhizal fungi cannot be cultured axenically, their Pi transporter network is difficult to exploite for large scale sustainable agriculture. Alternatively, the root-colonizing endophytic fungus Piriformospora indica can grow axenically and provides strong growth-promoting activity during its symbiosis with a broad spectrum of plants. P indica contains a high affinity Pi transporter (PiPT) involved in improving Pi nutrition levels in the host plant under P limiting conditions. As P indica can be manipulated genetically, it opens new vistas to be used in P deficient fields.
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  • Kumar Paras, Manoj, et al. (author)
  • ReTuna Recycling Mall : Reuse based Circular Fashion Supply Chain Management
  • 2016
  • Conference paper (other academic/artistic)abstract
    • The shopping mall concept has emerged to provide unique mall profiles to satisfy consumers who search for the ultimate shopping experience. Under one roof different sellers are assembled together with food outlets and entertainment to full fill the requirements of consumers.Gradually an awareness of over consumption has raised together with calls for reuse activities that reduce the consumption of new products. As an answer to this problem a shopping mall for sustainable practice and reuse: ReTuna, has been developed in Eskilstuna, Sweden.This study has been undertaken to understand the practice of ReTuna and the local based circular fashion supply chain that it incorporates. Still in its beginning ReTuna is indeed a revolutionary concept to enhance the practice of reuse.
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  • Pal, Rudrajeet, et al. (author)
  • Creating value through reverse logistics in a multi-echelon used clothing chain
  • 2016
  • In: Sustainable Transport and Supply Chain Innovation. - Kaohsiung, Taiwan. ; , s. 419-429
  • Conference paper (peer-reviewed)abstract
    • Purpose of this paper:Reverse logistics (RL) in retail value chains is an increasingly emerging phenomenon yet under-explored in research (Bernon et al., 2011). The literature becomes shallower while discussing the “process” of value creation in such context. Given the inherent complexity and differentiated value creation in many RL networks (Schenkel et al., 2015), e.g. in used clothing, such values are constituted by different actors by prioritizing and committing their strategic resources for developing distinct rent-earning competencies.In this context, the purpose of this paper is to explore how differential value is created by firms embedded in a multi-echelon reverse value chain for used clothing, by successfully exploiting multi-level (intra- and inter- firm) resources, via various underlying rent-earning mechanisms. Design/methodology/approach:An explorative case study approach is adopted in reverse clothing value chain context to investigate the take-back scheme that includes multiple actor types and also spans globally. An abductive research process is adopted along two stages; Stage 1 (proposes a new theoretical framework on “how” value is created in reverse value chains based on resource-based (RB) and relational rent-earning views to exploit various RL attributes or capabilities) and Stage 2 (seeks real-life case observations to explore the empirical reality), and finally systematically combining these knowledge.Data is collected through semi-structured interviews, observation and documented notes and reports, conducted with various actors, viz. retailers, social enterprises (charities and non-profit retailers), commercial brokers/sorters, and specialized sorting firms from India. Findings:Differentiated values are created by the actors involved with multi-echelon take-back network. The RB and relational theories underpin the rent-earning mechanisms further highlighting several key ways to sustain this value.The VRIO model in the RB theory (Barney and Clark, 2007) shows how value is created within firm boundaries. The relational view highlights four rent-earning mechanisms: relational asset specificity and information sharing for the success of cost-neutral take-back agreement, along with resource and capability complementarities and trust in the relationship. Together they provide understanding of the entire “process” of rent generation. Value:This research contribute to exploring the “process” of rent-earning generated by critical intra- and inter- organizational enablers of value creation in complex RL networks. Practical implications:The paper improves the understanding of the key mechanism for value creation for actors working within the used clothing chain. References:Barney, J., & Clark, D. (2007). Resource-Based Theory: Creating and Sustaining Competitive Advantage. Oxford: Oxford University Press.Bernon, M., Rossi, S., & Cullen, J. (2011). Retail reverse logistics: a call and grounding framework for research. International Journal of Physical Distribution & Logistics Management, 41(5), 484-510.Schenkel, M., Caniëls, M., Krikke, H., & van der Laanc, E. (2015). Understanding value creation in closed loop supply chains – Past findings and future directions. Journal of Manufacturing Systems. doi: http://dx.doi.org/10.1016/j.jmsy.2015.04.009
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  • Pal, Rudrajeet, Biträdande Professor, et al. (author)
  • Multidimensional value creation through different reverse supply chain relationships in used clothing sector
  • 2019
  • In: Supply chain management. - : Emerald Group Publishing Limited. - 1359-8546 .- 1758-6852. ; 24:6, s. 729-747
  • Journal article (peer-reviewed)abstract
    • PurposeThis paper aims to purport deeper understanding of, and instigate theoretical elaboration to, multidimensional value created through different reverse supply chain (RSC) relationships.Design/methodology/approachBy capturing the relationships (and their differences) constituted and embedded in three “extreme” case studies from global used clothing supply chain, the sources of multidimensional values are explored in line with Dyer and Singh’s (1998) relational theory.FindingsIn the RSC, when downstream relationships are typically more opportunistic, value is created using inter-personal ways of knowledge sharing and through use of informal safeguards. In contrast, the upstream RSC relationships are more symbiotic, and value is created through more seamless (and routinized) knowledge sharing practices, and additional use of more formal transaction-specific controls or financial incentives as safeguarding instruments.Research limitations/implicationsThe use of consolidated case studies may affect the consistency in the findings presented. Another limitation relates to deriving propositions per each source presented in relational theory.Practical implicationsPractitioners particularly from industries whose global RSCs include different natures of relationships and multiple value incentives can be benefited through this study.Originality/valueThe paper extends the original sources of value creation prescribed in relational theory by contextualizing them in RSCs. It depicts how multidimensional values are created relationally by dyadic partners as the nature of relationship differs between upstream and downstream.
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20.
  • Paras, Manoj Kumar, et al. (author)
  • A Romanian case study of clothes and accessories upcycling
  • 2019
  • In: Industria textila. ; 70:3, s. 285-290
  • Journal article (peer-reviewed)abstract
    • The present paper aims to investigate the practice of upcycling and redesign. The study draws on the multiple organizations involved in the redesigning activities. The organizations selected for the study are located in the northern part of Romania. Semi-structured interviews along with direct observations were used to collect information. The paper provides practical insights to upcycling process.Various kinds of redesigned products are made out of consumer and industrial wastes such as redesigned clothes, accessories for ladies, handbags, ladies purses and office stationery. Upcycling is generally considered as economically non-feasible. However, this study has found contradictory results. The demand-based redesign activities can help an organization to earn a profit. Two out of three selected organizations are able to self-sustain. One of the organizations is newly entered into the Romanian used clothing markets and ables to compete with existing players. This study could be seen as one of the early attempts to empirically explore the practice of textile and accessories upcycling practice in Eastern Europe. The findings from the current case study can provide several useful insights for other similar companies to make redesign activities profitable.
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21.
  • Paras, Manoj Kumar, et al. (author)
  • A state-of-the-art Literature Review of Upcycling: A Clothing Industry Perspective
  • 2016
  • In: CORTEP 2016 - Book of Abstracts. - Bucharest : Editura Acreditata de Cncsis Bucuresti. - 9786066854641 ; , s. 121-
  • Conference paper (peer-reviewed)abstract
    • Aim: The purpose of this study is to review and appreciate the developments in the literature of upcycling domain; (i) To comprehend the concept of upcycling and subsequently, understanding the difference among the prominent terminologies used in the literature (ii) To identify application of upcycling across various industries; (iii) To propose a framework of upcycling practices for clothing industries based on the insights. Methodology: A scientific literature review procedure proposed by Mayring (2002) was adopted to select and screen the paper which comprised of four steps; (i) Material collection: The collection of material is well defined and delimited based on the profiling approach. Each paper is defined as unit of analysis; (ii) Descriptive analysis: Different criteria are set to analyze collected materials. These are publication year, journal, methodology and author affiliations; (iii) Category selection: To do analysis different categories have been identified. Further those categories were divided into sub-categories; (iv) Material evaluation: According to above mentioned categorization, research papers are analyzed and interpreted to form a conceptual framework. Result: The paper has identified terminologies and definitions used in the literature. Recycling may be considered as  the use of the material properties (e.g. as a fire retardant non-woven material in a mattress spring cover) (Morley, Bartlett et al. 2009). Down-cycling may be conceptualized as making an inferior product or broken down into raw material. However, several scholars proposed various definitions of upcycling. The prominent may include: (i) Value/quality of product is improved by making superior product. (Dervojeda, Verzijl et al. 2014); (ii) Giving new value to materials that are either discarded, or are not being used anymore" (Fletcher and Grose 2012); (iii) repurposing lower-value items such as a neck scarf to construct a higher-value end use item, such as a wrap skirt or halter top (Janigo and Wu 2015). The results indicated that designing may be considered as one of the important steps in upcycling process. The process of redesigning consists of ideation, reconstruction and fitting. The limitation of redesigning is variability in size and pattern. This can be overcome by; craftsmanship, time, innovation, provenance, desire, narrative. Conclusion: The extant literature revealed that no study so far has attempted to summarize the literature in upcylcing area. Thus, this could be seen as a significant and unique contribution to the literature. Further, the bibliography and insights provided in the study may be used by future scholars as a ready reference for their research.
  •  
22.
  • Paras, Manoj Kumar, et al. (author)
  • A Sustainable Application Based on Grouping Genetic Algorithm for Modularized Redesign Model in Apparel Reverse Supply Chain
  • 2018
  • In: Sustainability. - : MDPI AG. - 2071-1050. ; 10:9
  • Journal article (peer-reviewed)abstract
    • The scarcity of natural resources and the problem of pollution have initiated the need for extending the life and use of existing products. The concept of the reverse supply chain provides an opportunity to recover value from discarded products. The potential for recovery and the improvement of value in the reverse supply chain of apparel has been barely studied. In this research, a novel modularized redesign model is developed and applied to the garment redesign process. The concept of modularization is used to extract parts from the end-of-use or end-of-life of products. The extracted parts are reassembled or reconstructed with the help of a proposed group genetic algorithm by using domain and industry-specific knowledge. Design fitness is calculated to achieve the optimal redesign. Subsequently, the practical relevance of the model is investigated with the help of an industrial case in Sweden. The case study finding reveals that the proposed method and model to calculate the design fitness could simplify the redesign process. The design fitness calculation is illustrated with the example of a polo t-shirt. The redesigned system-based modularization is in accordance with the practical situations because of its flexibility and viability to formulate redesign decisions. The grouping genetic algorithm could enable fast redesign decisions for designers.
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23.
  • Paras, Manoj Kumar, et al. (author)
  • An Exploratory Study of Swedish Charities to Develop a Model for the Reuse-Based Clothing Value Chain
  • 2018
  • In: Sustainability. - : MDPI AG. - 2071-1050. ; 10:4
  • Journal article (peer-reviewed)abstract
    • The present paper aims to explore the current clothes reuse business in order to develop a charity-driven model for the reuse-based clothing value chain. An exploratory study was carried out in Sweden to understand the business flow of clothes reuse. This study builds on the insights gained from the multiple charities involved in the reuse-based clothing value chain. Semi-structured interviews along with direct and participatory observation were used for data collection. In the current study of Swedish charities, the founders and senior managers of the organizations were interviewed. This paper provides several insights in the form of propositions and a model related to different drivers of the reuse-based clothing value chain. In this model, business factors (system, legislation, and awareness), product factors (design, quality, and price), and consumer attitude as donor/buyer are found to be key drivers. Product design, quality, and price depend upon clothes brand, construction, and material, which are collectively important for the sale of used products. In the future, researchers are encouraged to test the present set of propositions and the proposed model across different cultural settings. The model can serve as a framework for practitioners and will be helpful for designing business strategies based on the different factors identified in this study.
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24.
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25.
  • Paras, Manoj Kumar (author)
  • Application of fuzzy technique for closed loop decision in clothing value chain
  • 2018
  • In: International journal of value chain management. - 1741-5357 .- 1741-5365. ; 9:2, s. 105-121
  • Journal article (peer-reviewed)abstract
    • Increasing concern over the environment has encouraged consumer and industry to think about the huge amount of discarded clothes. This study has considered four methods: direct reuse, up-cycling, down-cycling and incineration to close the loop of clothing value chain. Three factors (material, economy and technology) affecting closed loop decision have been identified. These factors have a different degree of influences on the decision making the process of closed loop clothing value chain. Research data has been collected from the reverse logistic experts from clothing industry. The fuzzy technique has been used to quantify the response of experts. Findings have been analysed with the help of analytic hierarchy process technique. The material factor is found to dominating factor in the closed loop decisions over economy and technology. Up-cycling and direct reuse emerged to be the best alternatives in the current scenario. The clothing companies may replicate this approach of developing hierarchy model to choose best alternatives for the closed loop value chain.
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26.
  • Paras, Manoj Kumar, et al. (author)
  • Application of Markov chain for LCA: a study on the clothes 'reuse' in Nordic countries
  • 2017
  • In: The International Journal of Advanced Manufacturing Technology. - : Springer Science and Business Media LLC. - 0268-3768 .- 1433-3015.
  • Journal article (peer-reviewed)abstract
    • The purpose of this paper is to develop a model to count the number of cycles or trips that a clothing product could make in a reuse-based closed loop cycle. The model is primarily based on three scenarios: (i) self-reuse (ii) discard to second-hand market and (iii) disposed to incineration or the recycling stations. The present study extended and complemented the existing literature by presenting the application of the Markov chain to analyse the future of textile products on the basis of probabilities. Subsequently, the proposed model has been used to study the textile waste flow in the Nordic countries, i.e. Denmark, Finland, Iceland, Norway and Sweden. The application of the proposed model on the data from the Nordic Countries indicated that the average number of times the clothes reuse is highest in Denmark, whereas the lowest was found in Finland. Repair and redesign were found a hotspot for the recovery of clothes. Variation in these hotspots can increase the trip number of clothes. A sensitivity analysis is performed and conclusions are made regarding variations of clothes reuse under different scenarios. The proposed model may help in the decision formulation for the companies, government authorities and research agencies which focus on reuse and recycling of textile products. Based on the insights from the present work, the decision maker may take several initiatives to increase the life span of a textile product.
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27.
  • Paras, Manoj Kumar, et al. (author)
  • Developing a framework for the performance evaluation of sorting and grading firms of used clothing
  • 2018
  • In: Journal of Global Operations and Strategic Sourcing. - : Emerald Group Publishing Limited. - 2398-5364 .- 2398-5372.
  • Journal article (peer-reviewed)abstract
    • PurposeThis paper aims to propose a framework for evaluating the performance of reverse value chain activities in the clothing industry operating at base of the pyramid. Specifically, the research explores firm and supply chain factors influencing clothing reverse value chain activities with a focus on developing economies.Design/methodology/approachThe study adopted an explorative technique using direct observations and semi-structured interviews to collect information from eight companies and two traders. Internal resources and value chain capabilities were examined using theoretical underpinnings of resource-based view, transaction cost economics and base of the pyramid.FindingsThe paper identified multiple benefits of offshoring reverse value chain activities to the developing countries (at the base of the pyramid). Low operation cost, skilled manpower, business knowledge and location are found to be internal success factors. While favourable government legislation and domestic recycling markets are important external factors contributing to the success. Developing economies such as India contribute to firm performance by integrating, transforming, acquiring and co-creating the resources at base of the pyramid. Further, it was found that to achieve higher assets specificity, a few companies have opened their own shops in African countries, while others have opened sourcing branches in Canada or the USA to ensure good quality of raw materials. Collaboration and coordination among different value chain partners minimise cost and increases profitability. Innovation in the process such as clothes mutilation for recycling has created new business opportunities.Research limitations/implicationsInformation was collected from only eight organisations and two traders from India. Future scholars may extend the research to generalise the findings by documenting similar phenomena.Practical implicationsThe proposed framework can serve a basis for the practitioners to evaluate firm performance, and the insights can be used to achieve sustainability by engaging producers, employees, consumers and community using base of the pyramid approach.Originality/valueThe study provides unique insights into the prevalent export and re-exports phenomena of used clothing. The resource-based view, transaction cost economics and base of the pyramid strategy underpinned together to develop a framework for understanding reverse value chain activities of clothing.
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28.
  • Paras, Manoj Kumar, et al. (author)
  • ReTuna: The Recycling Mall
  • 2016
  • In: ReTuna: The Recycling Mall.
  • Conference paper (other academic/artistic)abstract
    • This study has been undertaken to understand the practice of ReTuna. This has been done through two phases: In the first phase backend operations have been studied by visiting collecting and sorting facilities and interviewing mall management. In the second phase each mall tenants have been studied and interviewed. After each interview data has been analysed and follow up interview were done to strengthen the information. The study shows that the reuse mall has been established near a recycling centre to promote the concept of reuse. Instead of disposing of goods to incineration, Citizens are encouraged to donate the goods to the mall. The collected products are sorted on the basis of type by mall employees and kept at different designated location of each tenants in line with their contract. Employees of the tenants visit the warehouse to receive and sort their assigned goods according to conditions and product categories. Some of the tenants have facilities to re-design, repair and wash the garments to improve the functionality of products. The mall management is doing efforts to increase the number of upcycling activities that the tenants perform to increase the value of the reused goods. The reuse mall also provides workshop and laboratory space to college involved in the education of reuse and re-design. Students of the college experiment with donated goods to redesign new products. 
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29.
  • Paras, Manoj Kumar, et al. (author)
  • Reuse based Closed Loop Clothing Value Chain: An Empirical investigation into Multinational Charities and Organizations of Norway, Sweden and UK
  • 2016
  • In: Reuse based Closed Loop Clothing Value Chain: An Empirical investigation into Multinational Charities and Organizations of Norway, Sweden and UK.
  • Conference paper (peer-reviewed)abstract
    • Purpose of this paper:The concept of closed loop value chain maximise the utilization of product. There are different ways to close the loop, reuse is most sustainable ways to do. Purpose of this paper are twofold; first to understand the activities of reuse based closed loop clothing value chain in Norway, Sweden and UK; and second to explore, how multinational charities and organizations in reuse based business can extract maximum values from discarded clothes. Design/methodology/approach:The present study adopted a single case study approach to understand the reverse logistics of clothes in three countries. Multiple sites were studied in different cultural environments to enhance the robustness of case study approach (Lau, 2012). Direct observation was made to understand the process in seven organizations. Eight face to face interviews were conducted with operation heads to understand the practices in-depth. These visits and interviews were made between September 2015 and February 2016. All interviews were transcribed and analysed with help of qualitative analysis software Nvivo 10. Different themes of value creation were categorised and used for cross-comparison of findings. Theoretical lens of resource based view has been used to understand to capability of a firm to extract maximum value from discarded clothes. In addition, we also examined the benefits of outsourcing different operations to supply chain partners in the absence of in-house facilities. Findings:Organizations endeavour to invent, re-invent and implement new ways to collect, sort and reprocess the used clothes. Collection events are organised in the different shopping malls to create awareness and increase the amount of collection. Contracts are signed with schools, local municipality, companies and postal departments to improve collection quality and quantity. After collection, the used garments are sorted and segregated into different categories. Sorting may be considered as one of the crucial stage which can provide competitive advantage to an organization in the second-hand clothing business (Ruiz-Torres, Ablanedo-Rosas, & Mukhopadhyay, 2013). Several firms had state-of-the-art machineries to simplify and handle the material management during sorting process efficiently. Some of the organizations are trying to have sensor based sophisticated technologies to reduce manual material handling. In contrast, a few companies have moved back to manual system to achieve high quality and productivity. Also, we observed that there is a scarcity of reprocessing facilities. As a result, a garment having small defects also gets mix up with discarded clothes. Some firms have small facility of washing and ironing, which is generally used for vintage garments. Success of an organization depends upon its ability to extract value from collected garments. Most of the firms are selling best quality of products in their own shops in the domestic markets while exporting inferior quality to developing counties. The second hand clothing companies are redefining their stores as an exclusive for vintage or redesign to improve domestic sales. Private labels have emerged to sale completely redesigned garments. Concept of selling products on the basis of weight in comparison to pieces has been adopted to move normal quality goods from retail stores. Some organizations have strengthened their own business network, hence they could get good price for the exported items. On the other hand, most of the selected firms export the used garments with the help of mediators located in Netherland, Belgium and Germany. The defected and damaged clothes are transported to energy station for incineration by paying small amount of user fees. A few organizations down cycle the damaged clothes to new products on their own or with the help of their supply chain partners. The reason that may be attributed is the lack of chemical and mechanical recycling facilities. A proper integration with recycling and rag making companies would enable organisations to obtain more value from textile waste and increase the revenue. Relevance/Contribution:The present study is an attempt to enhance the understanding of clothing reuse business in Norway, Sweden and UK. Every organisation try to acquire valuable and rare resources, like ‘one touch’ or sensors based sorting facility to get competitive advantage. The research also suggested that multinational charity and organisation performance mainly depends on its organizational ability to exploit resources available inside and outside the firms. Paras, Ekwall, and Pal (2015) case study focus on the local charity organisation those who have limited resources and organization skill. Resultantly value creation from collected goods is much less compare to value created by multinational charities and organisations. This can be implied that used clothing firms should focus on acquisition of valuable and rare resources to do effective collection and sorting. Firms should also organized resources within and outside firms by developing network throughout supply chain to maximise the revenue. This study is carried out in three European countries. It can be extended to other geographical region or more number of empirical evidence to achieve saturated result. References:Lau, K. H. (2012). Demand management in downstream wholesale and retail distribution: a case study. Supply Chain Management-an International Journal, 17(6), 638-654. doi:10.1108/13598541211269247Paras, M., Ekwall, D., & Pal, R. (2015). Testing a conceptual model of circular clothing value chain with product reuse in Swedish contest. Paper presented at the Global Cleaner Production & Sustainable Consumption Conference.Ruiz-Torres, a. J., Ablanedo-Rosas, J. H., & Mukhopadhyay, S. (2013). Supplier allocation model for textile recycling operations. International Journal of Logistics Systems and Management, 15(1), 108-124. doi:10.1504/IJLSM.2013.053241
  •  
30.
  • Paras, Manoj Kumar (author)
  • Reuse-based Reverse Value Chain for Sustainable Apparel Industry
  • 2018
  • Doctoral thesis (other academic/artistic)abstract
    • The reverse value chain is a concept that maximizes the utility of a product after end-of-life or end-of-use. Its main components are reuse, repair, up-cycling and down-cycling. This thesis has investigated the business of apparel ‘reuse’ to develop a reuse-based reverse value chain model for apparel industry. The research began by understanding the existing theory of reverse value chain. The theory includes a literature review of the value chain from the apparel industry as well as from the other industries. A conceptual framework has been developed by considering processes of reverse value chain such as collection, sorting and reprocessing.Consequently, the thesis has undertaken a mix method (qualitative and quantitative) approach to study the reuse-based reverse value chain. An exploratory method based on multiple case studies has been adopted to explore the current sustainable practices of apparel reuse. Organizations were visited and unstructured interviews were conducted with founder and senior managers. The results have been utilized to develop an empirical model from the current practices of collection, sorting, and reprocessing. Qualitative findings highlight that higher economic recovery depends upon efficient reprocessing and collaborations with different stakeholders of the reverse value chain of apparel. Thus, an efficient resource recovery is economical as well as environmentally sustainable. Engagement of marginalized and vulnerable sections of society in the reverse value chain of apparel contributes towards social sustainability.The empirical model was further strengthened by mathematical model formulation. Analytical hierarchy process, Genetic algorithm and Markov principle have been used for the analysis of reuse-based reverse value chain. The thesis provides theoretical contribution, implications and ways to improve the current practice of apparel reuse along with providing scope for future research.
  •  
31.
  • Paras, Manoj Kumar, et al. (author)
  • Revisiting upcycling phenomena: a concept in clothing industry
  • 2018
  • In: Research Journal of Textile and Apparel. - 1560-6074. ; 22:1, s. 46-58
  • Journal article (peer-reviewed)abstract
    • Purpose – The purpose of this study is to review the literature and practice of upcycling. In particular, the objective of this study is threefold: to comprehend the concept of upcycling and, subsequently, understanding the prominent terminologies used in the literature; to understand the process of upcycling and problem associated with it; and to review current literature and practice of upcycling for clothes.Design/methodology/approach – A scientific literature review procedure proposed by Mayring (2002)was adopted to select and screen the paper that comprises the following steps: material collection, descriptive analysis and material evaluation.Findings – Upcycling literature has witnessed significant contribution in the past one decade. The paper has identified various terminologies and definitions such as recycling, down-cycling, upcycling and redesign, which are used in the literature.Research limitations/implications – The present study may help the scholars to understand the current state of literature. A practitioner of upcycling can use the findings to improve and standardise the existing process.Originality/value – The process of redesigning is one of the important steps in upcycling, which comprises ideation, reconstruction and fitting. The limitation of redesigning is variability in size and pattern. This can be overcome through various techniques such as craftsmanship, time, innovation, provenance, desire and narrative.
  •  
32.
  • Paras, Manoj Kumar, et al. (author)
  • Systematic literature review to develop a conceptual framework for a reuse-based clothing value chain
  • 2017
  • In: The International Review of Retail, Distribution and Consumer Research. - : Taylor & Francis Group. - 0959-3969 .- 1466-4402. ; , s. 1-28
  • Journal article (peer-reviewed)abstract
    • A closed loop value chain is a concept that maximises a product’s utility both before and after end-of-life. This chain’s primary components are reuse, repair, up-cycling and down-cycling. This paper reviews the literature in the domain of ‘reuse’ to formulate and propose a conceptual framework for a ‘reuse-based clothing value chain’. We performed a systematic literature review in which a range of online databases were searched to select papers related to reuse between September 1994 and March 2015. Our review is presented broadly and in two parts: the first part provides a descriptive analysis of the articles, and the second part develops propositions based on the textual analysis. The review revealed that there are six primary drivers of the reuse-based clothing value chain: system, redesignability, price, information, legislation, and consumer attitude. Corresponding propositions highlight the key importance of system, product redesignability, product price, information, government legislation and consumer attitude to the economic success of the reuse-based clothing value chain. Finally, this work proposes a conceptual framework based on our propositions. This research may help scholars and practitioners to understand the current state of the literature. The list of references may be considered a source for future research in this area.
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33.
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34.
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35.
  • Reischke, Stephanie, et al. (author)
  • Threshold concentration of glucose for bacterial growth in soil
  • 2015
  • In: Soil Biology & Biochemistry. - : Elsevier BV. - 0038-0717. ; 80, s. 218-223
  • Journal article (peer-reviewed)abstract
    • The activity of heterotrophic soil microorganisms is usually limited by the availability and quality of carbon (C). Adding organic substances will thus trigger a microbial response. We studied the response in bacterial growth and respiration after the addition of low amounts of glucose. First we determined if additions of glucose, at concentrations which did not result in an exponential increase in respiration after the lag phase, still stimulated bacterial growth. The second aim was to determine the threshold concentration of glucose needed to induce bacterial growth. Adding glucose-C at 1000 mu g g(-1) soil resulted in an increased respiration rate, which was stable during 12 h, and then decreased without showing any exponential increase in respiration. Bacterial growth, determined as leucine incorporation, did not change compared to an unamended control during the first 12 h, but then increased to levels 5 times higher than in the control. Thus, after the lag phase, a period with increasing bacterial growth, but at the same time decreasing respiration rates, was found. Similar results, but with a more modest increase in bacterial growth, were found using 500 mu g glucose-C g(-1) soil. Adding 50-700 mu g glucose-C g(-1) resulted in increased respiration during 24 h correlating with the addition rate. In contrast, bacterial growth after 24 h was only stimulated by glucose additions >200 mu g C g(-1) soil. Thus, there was a threshold concentration of added substrate for inducing bacterial growth. Below the threshold concentration growth and respiration appear to be uncoupled. (C) 2014 Elsevier Ltd. All rights reserved.
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36.
  • Solanki, Manoj Kumar, et al. (author)
  • Shifts in the Composition of the Microbiota of Stored Wheat Grains in Response to Fumigation
  • 2019
  • In: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 10
  • Journal article (peer-reviewed)abstract
    • While the wheat-associated microbiome is of major agricultural importance, little is known about the alterations in wheat grain microbial community composition during storage. Characterization of the bacterial and fungal communities in stored wheat grains revealed the impact of phosphine fumigation, one of the most effective methods to eliminate insects in stored commodities, on the composition of the wheat grain microbiome. High-throughput amplicon sequencing of the bacterial 16S rRNA gene and fungal internal transcribed spacer (ITS) region was used to analyze the wheat grain microbiome at different times over as 6 months period of storage. Higher bacterial diversity was found across the samples during the first (immediately after harvest) and second (3 months later) time points, with a predominance of Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes and Planctomycetes. A two-fold decrease in the number of bacterial operational taxonomic units (OTUs) was observed in wheat grains at the last time point (6 months later), following phosphine treatment. In contrast to the effect of phosphine on bacteria, it did not affect fungal diversity in stored grains. The majority of fungal sequences were assigned to Ascomycota, followed by Basidiomycota, Glomeromycota, and unidentified fungi, which were evenly distributed throughout the storage period. Alpha and beta diversity analyses were confirmed by examination of the cultured microbial taxa obtained from the stored wheat grains. Mycotoxin analysis of wheat grains collected after phosphine fumigation revealed the presence of Fusarium toxins, primarily deoxynivalenol (DON). Several mycotoxigenic Fusarium spp. were also detected in the same samples. Results of the present study indicate that microbiome of stored, whole wheat grains was strongly affected by phosphine fumigation, which changed the structure of the microbial community leading to shifts in species composition toward mycotoxigenic strains. A better understanding of the complex interactions within the microbial communities of stored grains will assist in the development of novel biocontrol strategies to overcome mycotoxin contamination.
  •  
37.
  • Vovusha, Hakkim, et al. (author)
  • Binding Characteristics of Anticancer Drug Doxorubicin with Two-Dimensional Graphene and Graphene Oxide : Insights from Density Functional Theory Calculations and Fluorescence Spectroscopy
  • 2018
  • In: The Journal of Physical Chemistry C. - : AMER CHEMICAL SOC. - 1932-7447 .- 1932-7455. ; 122:36, s. 21031-21038
  • Journal article (peer-reviewed)abstract
    • There has been a perpetual interest in identifying suitable nano-carriers for drug delivery. In this regard, graphene-based two-dimensional materials have been proposed and demonstrated as drug carriers. In this paper, we have investigated the adsorption characteristics of a widely used anticancer drug, doxorubicin (DOX), on graphene (G) and graphene oxide (GO) by density functional theory calculations and fluorescence and X-ray photoelectron spectroscopies. From the calculated structural and electronic properties, we have concluded that G is a better binder of DOX compared to GO, which is also supported by our fluorescence measurements. The binding of DOX to G is mainly based on strong pi-pi stacking interactions. Consistent with this result, we also found that the sp(2) regions of GO interact with DOX stronger than the sp(3) regions attached with the functional groups; the binding is characterized by pi-pi and hydrogen-bonding interactions, respectively.
  •  
38.
  • Yadav, Manoj Kumar, et al. (author)
  • Adsorption and dissociation of dinitrogen on transition metal (Ta, W and Re) doped MgO surface
  • 2016
  • In: Computational and Theoretical Chemistry. - : Elsevier BV. - 2210-271X .- 2210-2728. ; 1090, s. 165-170
  • Journal article (peer-reviewed)abstract
    • The adsorption and dissociation of dinitrogen on transition metal (Ta, W and Re) doped MgO(100) surface has been studied employing density functional theory. It is found that all these transition metals (TM) on MgO(100) surface are capable of adsorbing dinitrogen (N-2), however there is no dissociative adsorption of N-2 on single transition metal dopant. When two TM atoms are doped on MgO(100) surface, dissociative adsorption of dinitrogen occurs in all the three cases. Whether the dissociation is spontaneous or is it associated with activation barrier depends on the orientation of N-2 molecule approaching the dopant site.
  •  
39.
  • Zhang, Xueyang, et al. (author)
  • Cellulose Synthase Stoichiometry in Aspen Differs from Arabidopsis and Norway Spruce
  • 2018
  • In: Plant Physiology. - : Oxford University Press (OUP). - 0032-0889 .- 1532-2548. ; 177, s. 1096-1107
  • Journal article (peer-reviewed)abstract
    • Cellulose is synthesized at the plasma membrane by cellulose synthase complexes (CSCs) containing cellulose synthases (CESAs). Genetic analysis and CESA isoform quantification indicate that cellulose in the secondary cell walls of Arabidopsis (Arabidopsis thaliana) is synthesized by isoforms CESA4, CESA7, and CESA8 in equimolar amounts. Here, we used quantitative proteomics to investigate whether the CSC model based on Arabidopsis secondary cell wall CESA stoichiometry can be applied to the angiosperm tree aspen (Populus tremula) and the gymnosperm tree Norway spruce (Picea abies). In the developing xylem of aspen, the secondary cell wall CESA stoichiometry was 3:2:1 for PtCESA8a/b:PtCESA4:PtCESA7a/b, while in Norway spruce, the stoichiometry was 1:1:1, as observed previously in Arabidopsis. Furthermore, in aspen tension wood, the secondary cell wall CESA stoichiometry changed to 8:3:1 for PtCESA8a/b:PtCESA4:PtCESA7a/b. PtCESA8b represented 73% of the total secondary cell wall CESA pool, and quantitative polymerase chain reaction analysis of CESA transcripts in cryosectioned tension wood revealed increased PtCESA8b expression during the formation of the cellulose-enriched gelatinous layer, while the transcripts of PtCESA4, PtCESA7a/b, and PtCESA8a decreased. A wide-angle x-ray scattering analysis showed that the shift in CESA stoichiometry in tension wood coincided with an increase in crystalline cellulose microfibril diameter, suggesting that the CSC CESA composition influences microfibril properties. The aspen CESA stoichiometry results raise the possibility of alternative CSC models and suggest that homomeric PtCESA8b complexes are responsible for cellulose biosynthesis in the gelatinous layer in tension wood.
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