| 1. |
- He, Qi, et al.
(författare)
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Pericyte dysfunction due to Shb gene deficiency increases B16F10 melanoma lung metastasis
- 2020
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Ingår i: International Journal of Cancer. - : Wiley. - 0020-7136 .- 1097-0215. ; 147:9, s. 2634-2644
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Tidskriftsartikel (refereegranskat)abstract
- Intravasation, vascular dissemination and metastasis of malignant tumor cells require their passage through the vascular wall which is commonly composed of pericytes and endothelial cells. We currently decided to investigate the relative contribution of these cell types to B16F10 melanoma metastasis in mice using an experimental model of host Shb gene (Src homology 2 domain containing protein B) inactivation. Conditional inactivation of Shb in endothelial cells using Cdh5-CreERt2 resulted in decreased tumor growth, reduced vascular leakage, increased hypoxia and no effect on pericyte coverage and lung metastasis. RNAseq of tumor endothelial cells from these mice revealed changes in cellular components such as adherens junctions and focal adhesions by gene ontology analysis that were in line with the observed effects on leakage and junction morphology. Conditional inactivation of Shb in pericytes using Pdgfrb-CreERt2 resulted in decreased pericyte coverage of small tumor vessels with lumen, increased leakage, aberrant platelet-derived growth factor receptor B (PDGFRB) signaling and a higher frequency of lung metastasis without concomitant effects on tumor growth or oxygenation. Flow cytometry failed to reveal immune cell alterations that could explain the metastatic phenotype in this genetic model of Shb deficiency. It is concluded that proper pericyte function plays a significant role in suppressing B16F10 lung metastasis.
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| 2. |
- Li, Fusheng, 1985-, et al.
(författare)
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Electroless Plating of NiFeP Alloy on the Surface of Silicon Photoanode for Efficient Photoelectrochemical Water Oxidation
- 2020
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Ingår i: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 12:10, s. 11479-11488
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Tidskriftsartikel (refereegranskat)abstract
- N- type silicon is a kind of semiconductor with a narrow band gap that has been reported as an outstanding light-harvesting material for photoelectrochemical (PEC) reactions. Decorating a thin catalyst layer on the n-type silicon surface can provide a direct and effective route toward PEC water oxidation. However, most of catalyst immobilization methods for reported n-type silicon photoanodes have been based on energetically demanding, time-consuming, and high-cost processes. Herein, a high-performance NiFeP alloy (NiFeP)-decorated n-type micro-pyramid silicon array (n-Si) photoanode (NiFeP/n-Si) was prepared by a fast and low-cost electroless deposition method for light-driven water oxidation reaction. The saturated photocurrent density of NiFeP/n-Si can reach up to similar to 40 mA cm(-2) and a photocurrent density of 15.5 mA cm(-2) can be achieved at 1.23 V-RHE under light illumination (100 mW cm(-2), AM1.5 filter), which is one of the most promising silicon-based photoanodes to date. The kinetic studies showed that the NiFeP on the silicon photoanodes could significantly decrease the interfacial charge recombination between the n-type silicon surface and electrolyte.
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| 3. |
- Wu, Xiujuan, et al.
(författare)
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Exploration of electrocatalytic water oxidation properties of NiFe catalysts doped with nonmetallic elements (P, S, Se)
- 2021
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Ingår i: International journal of hydrogen energy. - : Elsevier BV. - 0360-3199 .- 1879-3487. ; 46:79, s. 38992-39002
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Tidskriftsartikel (refereegranskat)abstract
- The introduction of non-metallic atoms (P, S, Se) has emerged as an effective way to improve the catalytic activity of transition metal based layered double hydroxides (LDHs) for oxygen evolution reaction. However, objective comparisons of the performance of heteroelement-doped catalysts are complicated by the lack of standardization both in the electrochemical tests and physicochemical analysis. Herein, we use a unified protocol for evaluating the catalytic activities of heteroelement-doped NiFe-LDHs and explore the reasons for the differences in their catalytic performance. Some regular results are found from comparing the properties of the heteroelement-doped catalysts: (1) the introduction of P/S/Se can optimize the redox behaviors of Ni species, which is conducive to regulating adsorption energy of intermediates and the formation of high-valent active sites; (2) the specific area of catalysts was expanded after heteroelements doping, ensuring a more favorable structure for heterogeneous catalysis; (3) All P/S/Se-doped catalysts showed better activities when compared to the original NiFe LDHs in alkaline solutions, and the catalysts doped with S showed the best performance (Se < P < S).
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| 4. |
- Fang, Zhiyong, et al.
(författare)
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Selective Electro-oxidation of Alcohols to the Corresponding Aldehydes in Aqueous Solution via Cu(III) Intermediates from CuO Nanorods
- 2021
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Ingår i: ACS Sustainable Chemistry and Engineering. - : American Chemical Society (ACS). - 2168-0485. ; 9:35, s. 11855-11861
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Tidskriftsartikel (refereegranskat)abstract
- Electrochemical oxidation using renewable energy is an attractive strategy that provides a sustainable and mild approach for biomass transformation. Herein, the electrocatalytic oxidation of furfuryl alcohol in an aqueous solution was investigated using CuO nanorods. Two kinds of Cu-III intermediates, namely, (CuO2)(-) and (Cu2O6)(6-), were detected on the surface of the working electrode. (Cu2O6)(6-), generated in the potential range of 1.35-1.39 V versus the reversible hydrogen electrode (RHE), induced the oxidation of furfuryl alcohol to furaldehyde with a yield of >= 98%. (CuO2)(-), generated at a potential greater than 1.39 V versus RHE, which led to the oxidation of furfuryl alcohol to 2-furoic acid with a yield of >= 99%. Furthermore, the Cu-III-catalyzed system exhibited a measure of universal applicability, wherein (Cu2O6)(6-) and (CuO2)(-) induced the highly selective electro-oxidation of benzyl alcohol, vanillyl alcohol, and 4-pyridinemethanol to yield the corresponding aldehydes and acids, respectively.
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| 5. |
- Li, Xiujuan, et al.
(författare)
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Suppressed Vascular Leakage and Myocardial Edema Improve Outcome From Myocardial Infarction
- 2020
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Ingår i: Frontiers in Physiology. - : Frontiers Media SA. - 1664-042X. ; 11
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Tidskriftsartikel (refereegranskat)abstract
- Aim: The acute phase of myocardial infarction (MI) is accompanied by edema contributing to tissue damage and disease outcome. Here, we aimed to identify the mechanism whereby vascular endothelial growth factor (VEGF)-A induces myocardial edema in the acute phase of MI to eventually promote development of therapeutics to specifically suppress VEGFA-regulated vascular permeability while preserving collateral vessel formation.Methods and Results: VEGFA regulates vascular permeability and edema by activation of VEGF receptor-2 (VEGFR2), leading to induction of several signaling pathways including the cytoplasmic tyrosine kinase c-Src. The activated c-Src in turn phosphorylates vascular endothelial (VE)-cadherin, leading to dissociation of endothelial adherens junctions. A particular tyrosine at position 949 in mouse VEGFR2 has been shown to be required for activation of c-Src. Wild-type mice and mice with phenylalanine replacing tyrosine (Y) 949 in VEGFR2 (Vegfr2Y949F/Y949F) were challenged with MI through permanent ligation of the left anterior descending coronary artery. The infarct size was similar in wild-type and mutant mice, but left ventricular wall edema and fibrinogen deposition, indicative of vascular leakage, were reduced in the Vegfr2Y949F/Y949F strain. When challenged with large infarcts, the Vegfr2Y949F/Y949F mice survived significantly better than the wild-type strain. Moreover, neutrophil infiltration and levels of myeloperoxidase were low in the infarcted Vegfr2Y949F/Y949F hearts, correlating with improved survival. In vivo tyrosine phosphorylation of VE-cadherin at Y685, implicated in regulation of vascular permeability, was induced by circulating VEGFA in the wild-type but remained at baseline levels in the Vegfr2Y949F/Y949F hearts.Conclusion: Suppression of VEGFA/VEGFR2-regulated vascular permeability leads to diminished edema without affecting vascular density correlating with improved myocardial parameters and survival after MI.
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| 6. |
- Sáinz-Jaspeado, Miguel, et al.
(författare)
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VE-cadherin junction dynamics in initial lymphatic vessels promotes lymph node metastasis
- 2024
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Ingår i: Life Science Alliance. - : Life Science Alliance. - 2575-1077. ; 7:3
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Tidskriftsartikel (refereegranskat)abstract
- The endothelial junction component vascular endothelial (VE)–cadherin governs junctional dynamics in the blood and lymphatic vasculature. Here, we explored how lymphatic junction stability is modulated by elevated VEGFA signaling to facilitate metastasis to sentinel lymph nodes. Zippering of VE-cadherin junctions was established in dermal initial lymphatic vessels after VEGFA injection and in tumor-proximal lymphatics in mice. Shape analysis of pan-cellular VE-cadherin fragments revealed that junctional zippering was accompanied by accumulation of small round-shaped VE-cadherin fragments in the lymphatic endothelium. In mice expressing a mutant VEGFR2 lacking the Y949 phosphosite (Vegfr2Y949F/Y949F) required for activation of Src family kinases, zippering of lymphatic junctions persisted, whereas accumulation of small VE-cadherin fragments was suppressed. Moreover, tumor cell entry into initial lymphatic vessels and subsequent metastatic spread to lymph nodes was reduced in mutant mice compared with WT, after challenge with B16F10 melanoma or EO771 breast cancer. We conclude that VEGFA mediates zippering of VE-cadherin junctions in initial lymphatics. Zippering is accompanied by increased VE-cadherin fragmentation through VEGFA-induced Src kinase activation, correlating with tumor dissemination to sentinel lymph nodes.
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| 7. |
- Wu, Xiujuan, et al.
(författare)
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Metalloid Te-Doped Fe-Based Catalysts Applied for Electrochemical Water Oxidation
- 2021
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Ingår i: CHEMISTRYSELECT. - : Wiley. - 2365-6549. ; 6:24, s. 6154-6158
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Tidskriftsartikel (refereegranskat)abstract
- Metal telluride nanocatalysts have been widely used in the catalytic reactions. However, there are few examples of electrocatalytic water oxidation with metal telluride as catalysts, especially Fe-based telluride, to the best of our knowledge, has not yet been reported. In this work, the Te-doped Fe-based catalysts (FeTex) with crystalline and amorphous nanosheet structures have been successfully synthesized by chemical vapor deposition (CVD) as well as electrodeposition (ED) on the iron foam (IF), where the latter exhibited more remarkable electrocatalytic performance towards the oxygen evolution reaction (OER) in the alkaline electrolyte, requiring the overpotential (eta) of only 264.4 mV to reach a current density of 10 mA cm(-2) with a Tafel slope of 54.2 mV dec(-1). The electrochemical study confirmed that the residual Te in ED-FeTex does play a role in promoting the catalytic activity for the electrocatalytic water oxidation.
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| 8. |
- Zhao, Ziqi, et al.
(författare)
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Molecular Engineering of Photocathodes based on Polythiophene Organic Semiconductors for Photoelectrochemical Hydrogen Generation
- 2021
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Ingår i: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 13:34, s. 40602-40611
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Tidskriftsartikel (refereegranskat)abstract
- Organic semiconductors provide significant potentials for the construction of photoelectrochemical (PEC) cells for solar hydrogen production because of their highly tunable properties. Herein, on carbon fiber paper (CFP) surface, pyridyl (Py), and 4,4'-bipyridin-1-ium (Py-2(+)) groups were introduced into polythiophene (pTH) semiconductor by electrochemical copolymerization, respectively. After assembly with the Co(dmgBF(2))(2) type catalyst (CoB, dmgBF(2) = difluoroboryldimethylglyoximate), the CoB@Py-2(+)-pTH/CFP photocathode displayed nearly twice the photocurrent enhancement (550 mu A cm(-2) at 0.15 V vs reversible hydrogen electrode, RHE) comparing to that generated by the CoB@Py-pTH/CFP photocathode (290 mu A cm(-2) at 0.15 V vs RHE) for light-driven H-2 generation under AM 1.5 solar illumination. Investigation of the mechanism revealed that the introduction of the positively charged pyridinium groups could improve the intrinsic Co(dmgBF(2))(2) catalyst activity for the H-2 generation reaction. Meanwhile, the positively charged pyridinium groups serve as p-type dopants to increase the semiconductor bulk charge transfer rate and act as electron transfer mediators to promote the interfacial charge transfer kinetics between the catalyst and the pTH-based organic semiconductor.
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