| 1. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 2. |
- Ablikim, M., et al.
(författare)
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Amplitude analysis of the D+ -> K-S(0)pi + (0)(pi) Dalitz plot
- 2014
-
Ingår i: Physical Review D. - 1550-7998 .- 1550-2368. ; 89:5, s. 052001-
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Tidskriftsartikel (refereegranskat)abstract
- We perform an analysis of the D+ -> K-S(0)pi + (0)(pi) Dalitz plot using a data set of 2.92 fb(-1) of e(+) e(-) collisions at the (3770) mass accumulated by the BESIII experiment, in which 166694 candidate events are selected with a background of 15.1%. The Dalitz plot is found to be well represented by a combination of six quasitwo- body decay channels [k(SP)(0)(+) (1450)(+,) ] plus a small nonresonant component. Using the fit fractions from this analysis, partial branching ratios are updated with higher precision than previous measurements.
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| 3. |
- Ablikim, M., et al.
(författare)
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Measurement of chi(cJ) decaying into eta ' K+K-
- 2014
-
Ingår i: Physical Review D. - 1550-7998 .- 1550-2368. ; 89:7, s. 074030-
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Tidskriftsartikel (refereegranskat)abstract
- Using (106.41 +/- 0.86) x 10(6) Psi(3686) events collected with the BESIII detector at BEPCII, we study for the first time the decay chi(cJ) -> eta'K+K- (J = 1, 2), where eta' -> gamma rho(0) and eta' -> eta pi(+)pi(-). A partial wave analysis in the covariant tensor amplitude formalism is performed for the decay chi(c1) -> eta'K+K-. Intermediate processes chi(c1) -> eta'f(2)'(1525) chi(c1) -> K-0*(1430)K-+/-(-/+) (K-0*(1430)(+/-) -> eta'K-+/-) are observed with statistical significances larger than 5 sigma, and their branching fractions are measured.
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| 4. |
- Ablikim, M., et al.
(författare)
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Measurement of the branching fraction for psi(3686) -> omega K+K-
- 2014
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Ingår i: Physical Review D. - 1550-7998 .- 1550-2368. ; 89:11, s. 112006-
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Tidskriftsartikel (refereegranskat)abstract
- With 1.06 x 10(8) psi(3686) events collected with the BESIII detector, the branching fraction of psi(3686) -> omega K+K- is measured to be (1.54 +/- 0.04 +/- 0.11) x 10(-4). This is the most precise result to date, due to the largest psi(3686) sample, improved signal reconstruction efficiency, good simulation of the detector performance, and a more accurate knowledge of the continuum contribution. Using the branching fraction of J/psi -> omega K+K-, the ratio B(psi(3868) -> K+K-)/B(J/psi -> K+K-) is determined to be (18.4 +/- 3.7)%. This constitutes a significantly improved test of the 12% rule, with the uncertainty now dominated by the J/psi branching fraction.
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| 5. |
- Ablikim, M., et al.
(författare)
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Measurement of the $D\to K^-\pi^+$ strong phase difference in $\psi(3770)\to D^0\overline{D}{}^0$
- 2014
-
Ingår i: PHYSICS LETTERS B. - : Elsevier BV. - 0370-2693. ; 734
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Tidskriftsartikel (refereegranskat)abstract
- We study $D^0\overline{D}{}^0$ pairs produced in $e^+e^-$ collisions at $\sqrt{s}=3.773$ GeV using a data sample of 2.92 fb$^{-1}$ collected with the BESIII detector. We measure the asymmetry $\mathcal{A}^{CP}_{K\pi}$ of the branching fractions of $D \to K^-\pi^+$ in $CP$-odd and $CP$-even eigenstates to be $(12.7\pm1.3\pm0.7)\times10^{-2}$. $\mathcal{A}^{CP}_{K\pi}$ can be used to extract the strong phase difference $\delta_{K\pi}$ between the doubly Cabibbo-suppressed process $\overline{D}{}^{0}\to K^-\pi^+$ and the Cabibbo-favored process $D^0\to K^- \pi^+$. Using world-average values of external parameters, we obtain $\cos\delta_{K\pi} = 1.02\pm0.11\pm0.06\pm0.01$. Here, the first and second uncertainties are statistical and systematic, respectively, while the third uncertainty arises from the external parameters. This is the most precise measurement of $\delta_{K\pi}$ to date.
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| 6. |
- Ablikim, M., et al.
(författare)
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Observation of e(+)e(-) -> gamma X(3872) at BESIII
- 2014
-
Ingår i: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 112:9, s. 092001-
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Tidskriftsartikel (refereegranskat)abstract
- With data samples collected with the BESIII detector operating at the BEPCII storage ring at center-of-mass energies from 4.009 to 4.420 GeV, the process e(+)e(-) -> gamma X(3872) is observed for the first time with a statistical significance of 6.3 sigma. The measured mass of the X(3872) is (3871.9 +/- 0.7(stat) +/- 0.2(syst)) MeV/c(2), in agreement with previous measurements. Measurements of the product of the cross section sigma[e(+)e(-) -> gamma X(3872)] and the branching fraction B [X(3872) -> pi(+)pi(-)J/psi] at center-of-mass energies 4.009, 4.229, 4.260, and 4.360 GeV are reported. Our measurements are consistent with expectations for the radiative transition process Y(4260) -> gamma X(3872).
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| 7. |
- Ablikim, M., et al.
(författare)
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Observation of e(+)e(-) -> pi(0)pi(0)h(c) and a Neutral Charmoniumlike Structure Z(c)(4020)(0)
- 2014
-
Ingår i: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 113:21, s. 212002-
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Tidskriftsartikel (refereegranskat)abstract
- Using data collected with the BESIII detector operating at the Beijing Electron Positron Collider at center-of-mass energies of root s = 4.23, 4.26, and 4.36 GeV, we observe e(+)e(-) -> pi(0)pi(0)h(c) for the first time. The Born cross sections are measured and found to be about half of those of e(+)e(-) -> pi(+)pi(-)h(c) within less than 2 sigma. In the pi(0)h(c) mass spectrum, a structure at 4.02 GeV/c(2) is found. It is most likely to be the neutral isospin partner of the Z(c)(4020)(+/-) observed in the process of e(+)e(-) -> pi(+)pi(-)h(c) being found. A fit to the pi(0)h(c) invariant mass spectrum, with the width of the Z(c)(4020)(0) fixed to that of its charged isospin partner and possible interferences with non-Z(c)(4020)(0) amplitudes neglected, gives a mass of (4023.9 +/- 2.2 +/- 3.8) MeV/c(2) for the Z(c)(4020)(0), where the first error is statistical and the second systematic.
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| 8. |
- Ablikim, M., et al.
(författare)
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Observation of $e^+e^− → γX$(3872) at BESIII
- 2014
-
Ingår i: PHYSICAL REVIEW LETTERS. - 1079-7114. ; 112:9
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Tidskriftsartikel (refereegranskat)abstract
- With data samples collected with the BESIII detector operating at the BEPCII storage ring at center-of-mass energies from 4.009 to 4.420 GeV, the process $e^{+} e^{-} \to \gamma X(3872)$ is observed for the first time with a statistical significance of $6.3\sigma$. The measured mass of the $X(3872)$ is ($3871.9\pm 0.7_{\rm stat.}\pm 0.2_{\rm sys.}$) MeV/$c^2$, in agreement with previous measurements. Measurements of the product of the cross section $\sigma[e^{+} e^{-} \to \gamma X(3872)]$ and the branching fraction $\mathcal{B}[X(3872) \to \pi^{+} \pi^{-} J/\psi]$ at center-of-mass energies 4.009, 4.229, 4.260, and 4.360 GeV are reported. Our measurements are consistent with expectations for the radiative transition process $Y(4260) \to \gamma X(3872)$.
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| 9. |
- Ablikim, M., et al.
(författare)
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Observation of electromagnetic Dalitz decays J/\psi \to P e^+e^-
- 2014
-
Ingår i: PHYSICAL REVIEW D. - 2470-0010 .- 1550-7998 .- 1550-2368. ; 89:9
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Tidskriftsartikel (refereegranskat)abstract
- Based on a sample of (225.3\pm2.8)\times 10^{6} J/\psi events collected with the BESIII detector, the electromagnetic Dalitz decays of J/\psi \to P e^+e^-(P=\eta'/\eta/\pi^0) are studied. By reconstructing the pseudoscalar mesons in various decay modes, the decays J/\psi \to \eta' e^+e^-, J/\psi \to \eta e^+e^- and J/\psi \to \pi^0 e^+e^- are observed for the first time. The branching fractions are determined to be \mathcal{B}(J/\psi\to \eta' e^+e^-) = (5.81\pm0.16\pm0.31)\times10^{-5}, \mathcal{B}(J/\psi\to \eta e^+e^-) = (1.16\pm0.07\pm0.06)\times10^{-5}, and \mathcal{B}(J/\psi\to \pi^0 e^+e^-)=(7.56\pm1.32\pm0.50)\times10^{-7}, where the first errors are statistical and the second ones systematic.
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| 10. |
- Ablikim, M., et al.
(författare)
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Observation of eta' -> pi(+) pi(-) pi(+) pi(-) and eta' -> pi(+) pi(-) pi(0) pi(0)
- 2014
-
Ingår i: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 112:25, s. 251801-
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Tidskriftsartikel (refereegranskat)abstract
- Using a sample of 1.3 x 10(9) J/psi events collected with the BESIII detector, we report the first observation of eta' -> pi(+) pi(-) pi(+) pi(-) and eta' -> pi(+) pi(-) pi(0) pi(0). The measured branching fractions are B(eta' -> pi(+) pi(-) pi(+) pi(-)) = [8.53 +/- 0.69(stat.) +/- 0.64(syst.)] x 10(-5) and B(eta' -> pi(+) pi(-) pi(0) pi(0)) = [1.82 +/- 0.35(stat.) +/- 0.18(syst.)] x 10(-4), which are consistent with theoretical predictions based on a combination of chiral perturbation theory and vector-meson dominance.
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| 11. |
- Ablikim, M., et al.
(författare)
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Observation of J/psi -> p(p)over-bara(0)(980) at BESIII
- 2014
-
Ingår i: Physical Review D. - 1550-7998 .- 1550-2368. ; 90:5, s. 052009-
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Tidskriftsartikel (refereegranskat)abstract
- Using 2.25 x 10(8) J/psi events collected with the BESIII detector at the BEPCII storage rings, we observe for the first time the process J/psi -> p (p) over bara(0)(980) -> pi(0)eta with a significance of 6.5 sigma (3.2 sigma including systematic uncertainties). The product branching fraction of J/psi -> p (p) over bara(0)(980) -> p (p) over bara(0)pi(0)eta is measured to be (6.8 +/- 1.2 +/- 1.3) x 10(-5), where the first error is statistical and the second is systematic. This measurement provides information on the a(0) production near threshold coupling to p (p) over bar and improves the understanding of the dynamics of J/psi decays to four-body processes.
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| 12. |
- Ablikim, M., et al.
(författare)
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Precision measurement of the mass of the tau lepton
- 2014
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Ingår i: Physical Review D. - 1550-7998 .- 1550-2368. ; 90:1
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Tidskriftsartikel (refereegranskat)abstract
- An energy scan near the tau pair production threshold has been performed using the BESIII detector. About 24 pb(-1) of data, distributed over four scan points, were collected. This analysis is based on t pair decays to ee, e mu, eh, h, hh, e.,. and p. final states, where h denotes a charged p or K. The mass of the t lepton is measured from a maximum likelihood fit to the t pair production cross- section data to be m(tau) = 1776.91 +/- 0.12_0.10 - 0.13 _ MeV/c(2), which is currently the most precise value in a single measurement.
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| 13. |
- Ablikim, M., et al.
(författare)
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Precision measurements of $B(D^+ \rightarrow \mu^+ \nu_{\mu})$, the pseudoscalar decay constant $f_{D^+}$, and the quark mixing matrix element $|V_{\rm cd}|$
- 2014
-
Ingår i: PHYSICAL REVIEW D. - 2470-0010 .- 1550-7998 .- 1550-2368. ; 89:5
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Tidskriftsartikel (refereegranskat)abstract
- We report a measurement of the branching fraction $B(D^+ \rightarrow \mu^+ \nu_{\mu}) = [3.71 \pm 0.19 (\rm stat) \pm 0.06 (\rm sys)]\times 10^{-4}$ based on 2.92 ${\rm fb^{-1}}$ of data accumulated at $\sqrt{s}=3.773$ GeV with the BESIII detector at the BEPCII collider. This measurement, in conjunction with the Cabibbo-Kobayashi-Maskawa matrix element $|V_{\rm cd}|$ determined from a global Standard Model fit, implies a value for the weak decay constant $f_{D^+}=(203.2 \pm 5.3 \pm 1.8)$ MeV. Additionally, using this branching fraction measurement together with a Lattice QCD prediction for $f_{D^+}$, we find $|V_{\rm cd}|=0.2210\pm 0.0058 \pm 0.0047$. In either case, these are the most precise results for these quantities to date.
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| 14. |
- Ablikim, M., et al.
(författare)
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Search for C-parity violation in J/psi -> gamma gamma and gamma phi
- 2014
-
Ingår i: Physical Review D. - 1550-7998 .- 1550-2368. ; 90:9, s. 092002-
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Tidskriftsartikel (refereegranskat)abstract
- Using 1.06 x 10(8) psi(3686) events recorded in e(+)e(-) collisions at root s = 3.686 GeV with the BESIII at the BEPCII collider, we present searches for C-parity violation in J/psi -> gamma gamma and gamma phi decays via psi(3686) -> J/psi pi(+)pi(-). No significant signals are observed in either channel. Upper limits on the branching fractions are set to be B(J/psi -> gamma gamma) < 2.7 x 10(-7) and B(J/psi -> gamma phi) < 1.4 x 10(-6) at the 90% confidence level. The former is one order of magnitude more stringent than the previous upper limit, and the latter represents the first limit on this decay channel.
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| 15. |
- Ablikim, M., et al.
(författare)
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Search for the radiative transitions Psi(3770) -> gamma eta(c) and gamma eta(c) (2S)
- 2014
-
Ingår i: Physical Review D. - 1550-7998 .- 1550-2368. ; 89:11, s. 112005-
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Tidskriftsartikel (refereegranskat)abstract
- By using a 2.92 fb-1 data sample taken at pffisffiffi 3.773 GeV with the BESIII detector operating at the BEPCII collider, we search for the radiative transitions.d3770c and cd2S through the hadronic decays cdcd2S. K0 SK p. No significant excess of signal events above background is observed. We set upper limits at a 90% confidence level for the product branching fractions to be Bdd3770cxBd.c. K0 SK k p < 1.6x10-5 and Bd.d3770cd2SxBd.cd2S. K0 SK p<5.6x10-6. Combining our result with world-average values of Bd.cd.cd2S. K0 SK p, we find the branching fractions Bd.d3770c< 6.8 x 10-4 and Bd.d3770cd2S< 2.0 x 10-3 at a 90% confidence level.
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| 16. |
- Ablikim, M., et al.
(författare)
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Search for the rare decays J/y -> D-s(-) rho(+) and J/psi -> <(D)over bar(0)<(K)over bar*(0)
- 2014
-
Ingår i: Physical Review D. - 1550-7998 .- 1550-2368. ; 89:7, s. 071101-
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Tidskriftsartikel (refereegranskat)abstract
- A search for the rare decays of J/psi -> D-S(-) rho(+) + c.c. and J/psi -> <(D)over bar(0)<(K)over bar*(0) + c.c. is performed with a data sample of 225.3-million J/psi events collected with the Beijing Spectrometer III detector. No evident signal is observed. Upper limits on the branching fractions are determined to be beta(J/psi -> D-S(-)rho(+) + c.c.) < 1.3 x 10(-5) and beta(J/psi -> <(D)over bar(0)<(K)over bar*(0) + c.c.) < 2.5 x 10(-6) at the 90% confidence level.
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| 17. |
- Ablikim, M., et al.
(författare)
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Search for the weak decays J/psi -> D-s(()*()-) e(+)nu(e) + c.c.
- 2014
-
Ingår i: Physical Review D. - 1550-7998 .- 1550-2368. ; 90:11, s. 112014-
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Tidskriftsartikel (refereegranskat)abstract
- Using a sample of 2.25 x 10(8) J/psi events collected with the BESIII detector at the BEPCII collider, we search for the J/psi semileptonic weak decay J/psi -> D-s(-) e(+)nu(e) +c.c. with a much higher sensitivity than previous searches. We also perform the first search for J/psi -> D-s(*-) e(+) nu(e) + c.c. No significant excess of a signal above background is observed in either channel. At the 90% confidence level, the upper limits are determined to be B(J/psi -> D-s(-) e(+) nu(e) + c.c.) < 1.3 x 10(-6) and B(J/psi -> D-s*(-) e(+) nu(e) + c.c.) < 1.8 x 10(-6), respectively. Both are consistent with Standard Model predictions.
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| 18. |
- Ablikim, M., et al.
(författare)
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Study of e(+)e(-) -> p(p)over-bar in the vicinity of psi(3770)
- 2014
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Ingår i: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 735, s. 101-107
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Tidskriftsartikel (refereegranskat)abstract
- Using 2917 pb(-1) of data accumulated at 3.773 GeV, 44.5 pb(-1) of data accumulated at 3.65 GeV and data accumulated during a psi(3770) line-shape scan with the BESIII detector, the reaction e(+)e(-) -> p (p) over bar is studied considering a possible interference between resonant and continuum amplitudes. The cross section of e(+)e(-) -> psi(3770) -> p (p) over bar, sigma(e(+)e(-)-> psi(3770) -> p (p) over bar), is found to have two solutions, determined to be (0.059(-0.020)(+0.070) +/- 0.012) pb with the phase angle phi = (255.8(-26.6)(+39.0) +/- 4.8). (< 0.166 pb at the 90% confidence level), or sigma(e(+)e(-) -> psi(3770) -> p<(p)over bar>) = (2.57(-0.13)(+0.12) +/- 0.12) pb with phi = (266.9(-6.3)(+6.1) +/- 0.9)degrees both of which agree with a destructive interference. Using the obtained cross section of psi(3770) -> p (p) over bar, the cross section of p (p) over bar -> psi(3770), which is useful information for the future PANDA experiment, is estimated to be either (9.8(-3.9)(+11.8)) nb (< 27.5 nb at 90% C.L.) or (425.6(-43.7)(+42.9)) nb. (C) 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license.
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| 19. |
- Ablikim, M., et al.
(författare)
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Study of e(+)e(-) -> p(p)over-bar pi(0) in the vicinity of the psi(3770)
- 2014
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Ingår i: Physical Review D. - 1550-7998 .- 1550-2368. ; 90:3, s. 032007-
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Tidskriftsartikel (refereegranskat)abstract
- The process e(+)e(-) -> p (p) over bar pi(0) has been studied by analyzing data collected at root s = 3.773 GeV, root s = 3.650 GeV, and during a psi(3770) line shape scan with the BESIII detector at the BEPCII collider. The Born cross section of p (p) over bar pi(0) in the vicinity of the psi(3770) is measured, and the Born cross section of psi(3770) -> p (p) over bar pi(0) is extracted considering interference between resonant and continuum production amplitudes. Two solutions with the same probability and a significance of 1.5 sigma are found. The solutions for the Born cross section of psi(3770) -> p (p) over bar pi(0) are 33.8 +/- 1.8 +/- 2.1 pb and 0.06(-0.04-0.01)(+0.10+0.01) pb (< 0.22 pb at a 90% confidence level). Using the estimated cross section and a constant decay amplitude approximation, the cross section sigma(p<(p)over bar> -> psi(3770)pi(0)) is calculated for the kinematic situation of the planned (p) over bar ANDA experiment. The maximum cross section corresponding to the two solutions is expected to be less than 0.79 nb at 90% confidence level and 122 +/- 10 nb at a center-of-mass energy of 5.26 GeV.
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| 20. |
- Zhang, Liang, et al.
(författare)
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An experimental investigation of a natural circulation heat pipe system applied to a parabolic trough solar collector steam generation system
- 2012
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Ingår i: Solar Energy. - : Elsevier BV. - 0038-092X .- 1471-1257. ; 86:3, s. 911-919
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Tidskriftsartikel (refereegranskat)abstract
- A U-type natural circulation heat pipe system is designed and applied to a parabolic trough solar collector for generating mid-temperature steam. Thermal performance of the heat pipe system is investigated experimentally. A detailed heat transfer analysis is performed on thermal behaviors of the system, especially the solar collector. The results show that the system can generate mid-temperature steam of a pressure up to 0.75 MPa. The thermal efficiency is found to be 38.52% at discharging pressure of 0.5 MPa during summer time.
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| 21. |
- Zhang, Liang, et al.
(författare)
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An experimental investigation of the heat losses of a U-type solar heat pipe receiver of a parabolic trough collector-based natural circulation steam generation system
- 2013
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Ingår i: Renewable energy. - : Elsevier BV. - 0960-1481 .- 1879-0682. ; 57, s. 262-268
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Tidskriftsartikel (refereegranskat)abstract
- The performance of a parabolic trough collector (PTC)-based steam generation system depends significantly on the heat losses of the solar receiver. This paper presents an experimental study of the heat losses of a double glazing vacuum U-type solar receiver mounted in a PTC natural circulation system for generating medium-temperature steam. Field experiments were performed to determine the overall heat losses of the receiver. Effects of wind, vacuum glass tube, radiation, and structural characteristics on the heat losses were analyzed. The thermal efficiency of the receiver was found to be 0.791 and 0.472 in calm and windy days, respectively, at a test temperature of about 100 degrees C, whereas the thermal efficiencies became 0.792 and 0.663, respectively, while taking the receiver element into consideration. The heat losses were increased from 0.183 to 0.255 kW per receiver for the two cases tested. It was shown that neither convection nor radiation heat losses may be negligible in the analysis of such U-type solar receivers.
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| 22. |
- Chen, Dongfeng, et al.
(författare)
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Glioma Cell Proliferation Controlled by ERK Activity-Dependent Surface Expression of PDGFRA.
- 2014
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Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 9:1
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Tidskriftsartikel (refereegranskat)abstract
- Increased PDGFRA signaling is an essential pathogenic factor in many subtypes of gliomas. In this context the cell surface expression of PDGFRA is an important determinant of ligand sensing in the glioma microenvironment. However, the regulation of spatial distribution of PDGFRA in glioma cells remains poorly characterized. Here, we report that cell surface PDGFRA expression in gliomas is negatively regulated by an ERK-dependent mechanism, resulting in reduced proliferation of glioma cells. Glioma tumor tissues and their corresponding cell lines were isolated from 14 patients and analyzed by single-cell imaging and flow cytometry. In both cell lines and their corresponding tumor samples, glioma cell proliferation correlated with the extent of surface expression of PDGFRA. High levels of surface PDGFRA also correlated to high tubulin expression in glioma tumor tissue in vivo. In glioma cell lines, surface PDGFRA declined following treatment with inhibitors of tubulin, actin and dynamin. Screening of a panel of small molecule compounds identified the MEK inhibitor U0126 as a potent inhibitor of surface PDGFRA expression. Importantly, U0126 inhibited surface expression in a reversible, dose- and time-dependent manner, without affecting general PDGFRA expression. Treatment with U0126 resulted in reduced co-localization between PDGFRA and intracellular trafficking molecules e.g. clathrin, RAB11 and early endosomal antigen-1, in parallel with enhanced co-localization between PDGFRA and the Golgi cisternae maker, Giantin, suggesting a deviation of PDGFRA from the endosomal trafficking and recycling compartment, to the Golgi network. Furthermore, U0126 treatment in glioma cells induced an initial inhibition of ERK1/2 phosphorylation, followed by up-regulated ERK1/2 phosphorylation concomitant with diminished surface expression of PDGFRA. Finally, down-regulation of surface PDGFRA expression by U0126 is concordant with reduced glioma cell proliferation. These findings suggest that manipulation of spatial expression of PDGFRA can potentially be used to combat gliomas.
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| 23. |
- Chen, Yong, et al.
(författare)
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A Vps21 endocytic module regulates autophagy
- 2014
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Ingår i: Molecular Biology of the Cell. - 1939-4586. ; 25:20, s. 3166-3177
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Tidskriftsartikel (refereegranskat)abstract
- In autophagy, the double-membrane autophagosome delivers cellular components for their degradation in the lysosome. The conserved Ypt/Rab GTPases regulate all cellular trafficking pathways, including autophagy. These GTPases function in modules that include guanine-nucleotide exchange factor (GEF) activators and downstream effectors. Rab7 and its yeast homologue, Ypt7, in the context of such a module, regulate the fusion of both late endosomes and autophagosomes with the lysosome. In yeast, the Rab5-related Vps21 is known for its role in early- to late-endosome transport. Here we show an additional role for Vps21 in autophagy. First, vps21Δ mutant cells are defective in selective and nonselective autophagy. Second, fluorescence and electron microscopy analyses show that vps21Δ mutant cells accumulate clusters of autophagosomal structures outside the vacuole. Third, cells with mutations in other members of the endocytic Vps21 module, including the GEF Vps9 and factors that function downstream of Vps21, Vac1, CORVET, Pep12, and Vps45, are also defective in autophagy and accumulate clusters of autophagosomes. Finally, Vps21 localizes to PAS. We propose that the endocytic Vps21 module also regulates autophagy. These findings support the idea that the two pathways leading to the lysosome—endocytosis and autophagy—converge through the Vps21 and Ypt7 GTPase modules.
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| 24. |
- Kooner, Jaspal S, et al.
(författare)
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Genome-wide association study in individuals of South Asian ancestry identifies six new type 2 diabetes susceptibility loci.
- 2011
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Ingår i: Nature Genetics. - : Springer Science and Business Media LLC. - 1061-4036 .- 1546-1718. ; 43:10
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Tidskriftsartikel (refereegranskat)abstract
- We carried out a genome-wide association study of type-2 diabetes (T2D) in individuals of South Asian ancestry. Our discovery set included 5,561 individuals with T2D (cases) and 14,458 controls drawn from studies in London, Pakistan and Singapore. We identified 20 independent SNPs associated with T2D at P < 10(-4) for testing in a replication sample of 13,170 cases and 25,398 controls, also all of South Asian ancestry. In the combined analysis, we identified common genetic variants at six loci (GRB14, ST6GAL1, VPS26A, HMG20A, AP3S2 and HNF4A) newly associated with T2D (P = 4.1 × 10(-8) to P = 1.9 × 10(-11)). SNPs at GRB14 were also associated with insulin sensitivity (P = 5.0 × 10(-4)), and SNPs at ST6GAL1 and HNF4A were also associated with pancreatic beta-cell function (P = 0.02 and P = 0.001, respectively). Our findings provide additional insight into mechanisms underlying T2D and show the potential for new discovery from genetic association studies in South Asians, a population with increased susceptibility to T2D.
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| 25. |
- Lu, Hai, et al.
(författare)
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Parametric analysis of energy quality management for district in China using multi-objective optimization approach
- 2014
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Ingår i: Energy Conversion and Management. - : Elsevier BV. - 0196-8904 .- 1879-2227. ; 87, s. 636-646
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Tidskriftsartikel (refereegranskat)abstract
- Due to the increasing energy demands and global warming, energy quality management (EQM) for districts has been getting importance over the last few decades. The evaluation of the optimum energy systems for specific districts is an essential part of EQM. This paper presents a deep analysis of the optimum energy systems for a district sited in China. A multi-objective optimization approach based on Genetic Algorithm (GA) is proposed for the analysis. The optimization process aims to search for the suitable 3E (minimum economic cost and environmental burden as well as maximum efficiency) energy systems. Here, life cycle CO2 equivalent (LCCO2), life cycle cost (LCC) and exergy efficiency (EE) are set as optimization objectives. Then, the optimum energy systems for the Chinese case are presented. The final work is to investigate the effects of different energy parameters. The results show the optimum energy systems might vary significantly depending on some parameters.
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| 26. |
- Na, Manli, et al.
(författare)
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Adenovirus assembly is impaired by BMI1-related histone deacetylase activity.
- 2014
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Ingår i: Virology. - : Elsevier BV. - 1096-0341 .- 0042-6822. ; 456:Apr 17, s. 227-237
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Tidskriftsartikel (refereegranskat)abstract
- Polycomb ring finger oncogene BMI1 (B cell-specific Moloney murine leukemia virus integration site 1) plays a critical role in development of several types of cancers. Here, we report an inverse relationship between levels of BMI1 expression and adenovirus (Ad) progeny production. Enforced BMI1 expression in A549 cells impaired Ad progeny production. In contrast, knocking-down of endogenous BMI1 expression enhanced progeny production of a conditionally replicating Ad and wild-type Ad5 and Ad11p. Ad vectors overexpressing BMI1 were not impaired in the replication of progeny genomes and in the expression of E1A and Ad structural proteins. However, 293 cells infected by Ad vector overexpressing BMI1 contained a large proportion of morphologically irregular Ad particles. This effect was reversed in 293 cells pre-treated with the histone deacetylase (HDAC) inhibitor trichostatin A (TSA) in parallel with the production of infectious Ad particles. Our findings suggest an inhibitory role of BMI1 in Ad morphogenesis that can be implied in Ad tropism and Ad-mediated cancer therapy.
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| 27. |
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| 28. |
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| 29. |
- Sun, M-B, et al.
(författare)
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Flame stabilization in a supersonic combustor with hydrogen injection upstream of cavity flame holders: experiments and simulations
- 2011
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Ingår i: Proceedings of the Institution of Mechanical Engineers. Part G: Journal of Aerospace Engineering. - : SAGE Publications. - 0954-4100 .- 2041-3025. ; 225:G12, s. 1351-1365
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Tidskriftsartikel (refereegranskat)abstract
- Experimental observations and numerical simulation were conducted to study the flame characteristics and flame stabilization mechanism in a supersonic combustor with hydrogen injection upstream of cavity flame holders. OH radical distribution of the reacting flowfield was obtained using OH spontaneous emission and OH-PLIF (planar laser-induced fluorescence). The supersonic combustion flowfield with L/D = 7 cavity was calculated by large eddy simulation. The turbulence-combustion interaction model was based on a partially premixed flamelet model with a level-set approach. The results showed that hydrogen fuels were transported into the cavity shear; lean mixture and rich mixture were produced in the internal cavity and the declining jet, respectively. An approximately steady partially premixed flame front exists in the cavity shear layer. The flame front propagates and extends to the region around the fuel jet due to the interaction of counter-rotating vortices induced by the jet with the cavity shear layer. The flame front sustained in the shear layer likely penetrates the jet core and ignites the whole jet. Behind the flame front, most of the jet beam is burned as diffusion flames. The physical process of the flame stabilization demonstrated the similarity with triple flame characteristics, which indicted that triple flame theory might be the basic flame stabilization mechanism of the cavity flame holders.
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| 30. |
- Sun, Yingyu, et al.
(författare)
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A glioma classification scheme based on coexpression modules of EGFR and PDGFRA
- 2014
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424. ; 111:9, s. 3538-3543
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Tidskriftsartikel (refereegranskat)abstract
- We hypothesized that key signaling pathways of glioma genesis might enable the molecular classification of gliomas. Gene coexpression modules around epidermal growth factor receptor (EGFR) (EM, 29 genes) or platelet derived growth factor receptor A (PDGFRA) (PM, 40 genes) in gliomas were identified. Based on EM and PM expression signatures, nonnegative matrix factorization reproducibly clustered 1,369 adult diffuse gliomas WHO grades II-IV from four independent databases generated in three continents, into the subtypes (EM, PM and EMlowPMlow gliomas) in a morphology-independent manner. Besides their distinct patterns of genomic alterations, EM gliomas were associated with higher age at diagnosis, poorer prognosis, and stronger expression of neural stem cell and astrogenesis genes. Both PM and EMlowPMlow gliomas were associated with younger age at diagnosis and better prognosis. PM gliomas were enriched in the expression of oligodendrogenesis genes, whereas EMlowPMlow gliomas were enriched in the signatures of mature neurons and oligodendrocytes. The EM/PM-based molecular classification scheme is applicable to adult low-grade and high-grade diffuse gliomas, and outperforms existing classification schemes in assigning diffuse gliomas to subtypes with distinct transcriptomic and genomic profiles. The majority of the EM/PM classifiers, including regulators of glial fate decisions, have not been extensively studied in glioma biology. Subsets of these classifiers were coexpressed in mouse glial precursor cells, and frequently amplified or lost in an EM/PM glioma subtypespecific manner, resulting in somatic copy number alteration-dependent gene expression that contributes to EM/PM signatures in glioma samples. EM/PM-based molecular classification provides a molecular diagnostic framework to expedite the search for new glioma therapeutic targets.
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| 31. |
- Wang, Xiaolong, et al.
(författare)
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Carrier Frequency Offset Estimation in the Presence of I/Q Mismatch for Wideband OFDM Systems
- 2012
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Ingår i: IEEE 55th International Midwest Symposium on Circuits and Systems (MWSCAS), 2012. - 9781467325264 ; , s. 924-927
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Konferensbidrag (refereegranskat)abstract
- This paper presents a data-aided Carrier Frequency Offset (CFO) estimation algorithm targeting the Wideband OFDM systems. Utilizing 3 consecutive preambles and processing them at frequency domain, the algorithm is robust to both Frequency Independent (F-I) and Frequency Dependent (F-D) I/Q Mismatch (I/Q-M). To evaluate the effectiveness of the proposed CFO estimation technique, we conduct extensive simulations based on Multi-Band OFDM Ultra-Wide Band wireless transmission. Compared to the ideal case when the CFO has been perfectly estimated, the performance loss at 8% Packet Error Rate (PER) is less than 0.5 dB.
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| 32. |
- Weinstein, John N., et al.
(författare)
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The cancer genome atlas pan-cancer analysis project
- 2013
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Ingår i: Nature Genetics. - : Springer Science and Business Media LLC. - 1061-4036 .- 1546-1718. ; 45:10, s. 1113-1120
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Forskningsöversikt (refereegranskat)abstract
- The Cancer Genome Atlas (TCGA) Research Network has profiled and analyzed large numbers of human tumors to discover molecular aberrations at the DNA, RNA, protein and epigenetic levels. The resulting rich data provide a major opportunity to develop an integrated picture of commonalities, differences and emergent themes across tumor lineages. The Pan-Cancer initiative compares the first 12 tumor types profiled by TCGA. Analysis of the molecular aberrations and their functional roles across tumor types will teach us how to extend therapies effective in one cancer type to others with a similar genomic profile. © 2013 Nature America, Inc. All rights reserved.
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| 33. |
- Yang, Tien-Nan, et al.
(författare)
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Variations in monsoonal rainfall over the last 21 kyr inferred from sedimentary organic matter in Tung-Yuan Pond, southern Taiwan
- 2011
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Ingår i: Quaternary Science Reviews. - : Elsevier BV. - 0277-3791 .- 1873-457X. ; 30:23-24, s. 3413-3422
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Tidskriftsartikel (refereegranskat)abstract
- Changes in paleorainfall intensity linked to the strength of the East Asian (EA) summer monsoon since 21 cal kyr BP are inferred from the organic matter contents of a 15-m sediment core from Tung-Yuan Pond in southern Taiwan. High total organic carbon/total nitrogen (TOC/TN) values in association with increased TOC content suggest that more soil-derived material containing terrestrial organic matter (OM) was delivered to the lake during periods of increased runoff associated with extensive precipitation that resulted from intensified summer monsoons, whereas low values indicate OM possessing a dominant algal origin during weakened summer monsoons. Rainfall intensity in terms of the proportion of terrestrial OM was high in four periods: the last deglaciation (similar to 17.2 to similar to 12.2 ka), the early Holocene (similar to 10.6 to similar to 8.6 ka), the middle Holocene Thermal Optimum (similar to 7.7 to similar to 5 ka) and the late Holocene (similar to 4.2 to similar to 2 ka), whereas it was low in the intervening time periods. High TOC/TN values coincide with peak values of summer insolation, and thus the strongest EA summer monsoon during the early and middle Holocene: small drops in these ratios correspond to increasing and decreasing solar radiation in the deglacial period and the late Holocene, respectively. The four intervals with low TOC/TN ratios, as well as episodic drops of the ratios during the deglaciation and the early and late Holocene are concordant with the late last glacial (similar to 21 to similar to 17.2 ka), the Oldest (similar to 14.8 ka), the Older (similar to 13.3 ka) and the Younger Dryas (similar to 13 to similar to 11.5 ka), the 8.2 cold event (similar to 8.6-7.7 ka) and a late Holocene cold event (similar to 5-4.2 ka), and suggest a weakened EA summer monsoon during these times. Moreover, high frequency hydrological variability occurred during the early Holocene, heavy rainfall persisted during the middle Holocene, and precipitation intensity generally diminished after similar to 5 ka. The Tung-Yuan Pond sediment record indicates that the TOC/TN ratio can be used as a paleorainfall intensity proxy to trace variations in the EA summer monsoon strength in other small lakes. Crown Copyright
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| 34. |
- Zhang, S. N., et al.
(författare)
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The high energy cosmic-radiation detection (HERD) facility onboard China's Space Station
- 2014
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Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - : SPIE. - 9780819496126
-
Konferensbidrag (refereegranskat)abstract
- The High Energy cosmic-Radiation Detection (HERD) facility is one of several space astronomy payloads of the cosmic lighthouse program onboard China's Space Station, which is planned for operation starting around 2020 for about 10 years. The main scientific objectives of HERD are indirect dark matter search, precise cosmic ray spectrum and composition measurements up to the knee energy, and high energy gamma-ray monitoring and survey. HERD is composed of a 3-D cubic calorimeter (CALO) surrounded by microstrip silicon trackers (STKs) from five sides except the bottom. CALO is made of about 104 cubes of LYSO crystals, corresponding to about 55 radiation lengths and 3 nuclear interaction lengths, respectively. The top STK microstrips of seven X-Y layers are sandwiched with tungsten converters to make precise directional measurements of incoming electrons and gamma-rays. In the baseline design, each of the four side SKTs is made of only three layers microstrips. All STKs will also be used for measuring the charge and incoming directions of cosmic rays, as well as identifying back scattered tracks. With this design, HERD can achieve the following performance: energy resolution of 1% for electrons and gamma-rays beyond 100 GeV, 20% for protons from 100 GeV to 1 PeV; electron/proton separation power better than 10-5; effective geometrical factors of >3 m2sr for electron and diffuse gamma-rays, >2 m2sr for cosmic ray nuclei. R and D is under way for reading out the LYSO signals with optical fiber coupled to image intensified CCD and the prototype of one layer of CALO.
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| 35. |
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| 36. |
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| 37. |
- Zhu, Lin, et al.
(författare)
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Immunization with advanced glycation end products modified low density lipoprotein inhibits atherosclerosis progression in diabetic apoE and LDLR null mice
- 2014
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Ingår i: Cardiovascular Diabetology. - : Springer Science and Business Media LLC. - 1475-2840. ; 13
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Tidskriftsartikel (refereegranskat)abstract
- Background: Diabetes accelerates atherosclerosis through undefined molecular mechanisms. Hyperglycemia induces formation of advanced glycation end product (AGE)-modified low-density lipoprotein (LDL). Anti-AGE-LDL autoantibodies favor atherosclerosis (AS) progression in humans, while anti oxidized LDL immunization inhibits AS in hypercholesterolemic, non-diabetic mice. We here investigated if AGE-LDL immunization protects against AS in diabetic mice. Methods: After diabetes induction with streptozotocin and high fat diet, both low density lipoprotein receptor (LDLR)(-/-) and apoE female mice were randomized to: AGE-LDL immunization with aluminum hydroxide (Alum) adjuvant; Alum alone; or PBS. Results: AGE-LDL immunization: significantly reduced AS; induced specific plasma IgM and IgG antibodies; upregulated splenic Th2, Treg and IL-10 levels, without altering Th1 or Th17 cells; and increased serum high density lipoprotein(HDL) while numerically lowering HbA1c levels. Conclusions: Subcutaneous immunization with AGE-LDL significantly inhibits atherosclerosis progression in hyperlipidemic diabetic mice possibly through activation of specific humoral and cell mediated immune responses and metabolic control improvement.
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