| 1. |
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| 2. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 3. |
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| 4. |
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| 5. |
- Jin, Ying-Hui, et al.
(författare)
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Chemoprophylaxis, diagnosis, treatments, and discharge management of COVID-19 : An evidence-based clinical practice guideline (updated version)
- 2020
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Ingår i: Military Medical Research. - : Springer Science and Business Media LLC. - 2054-9369. ; 7:1
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Tidskriftsartikel (refereegranskat)abstract
- The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of a rapidly spreading illness, coronavirus disease 2019 (COVID-19), affecting more than seventeen million people around the world. Diagnosis and treatment guidelines for clinicians caring for patients are needed. In the early stage, we have issued "A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus (2019-nCoV) infected pneumonia (standard version)"; now there are many direct evidences emerged and may change some of previous recommendations and it is ripe for develop an evidence-based guideline. We formed a working group of clinical experts and methodologists. The steering group members proposed 29 questions that are relevant to the management of COVID-19 covering the following areas: chemoprophylaxis, diagnosis, treatments, and discharge management. We searched the literature for direct evidence on the management of COVID-19, and assessed its certainty generated recommendations using the Grading of Recommendations, Assessment, Development and Evaluation (GRADE) approach. Recommendations were either strong or weak, or in the form of ungraded consensus-based statement. Finally, we issued 34 statements. Among them, 6 were strong recommendations for, 14 were weak recommendations for, 3 were weak recommendations against and 11 were ungraded consensus-based statement. They covered topics of chemoprophylaxis (including agents and Traditional Chinese Medicine (TCM) agents), diagnosis (including clinical manifestations, reverse transcription-polymerase chain reaction (RT-PCR), respiratory tract specimens, IgM and IgG antibody tests, chest computed tomography, chest x-ray, and CT features of asymptomatic infections), treatments (including lopinavir-ritonavir, umifenovir, favipiravir, interferon, remdesivir, combination of antiviral drugs, hydroxychloroquine/chloroquine, interleukin-6 inhibitors, interleukin-1 inhibitors, glucocorticoid, qingfei paidu decoction, lianhua qingwen granules/capsules, convalescent plasma, lung transplantation, invasive or noninvasive ventilation, and extracorporeal membrane oxygenation (ECMO)), and discharge management (including discharge criteria and management plan in patients whose RT-PCR retesting shows SARS-CoV-2 positive after discharge). We also created two figures of these recommendations for the implementation purpose. We hope these recommendations can help support healthcare workers caring for COVID-19 patients.
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| 6. |
- Kanoni, Stavroula, et al.
(författare)
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Implicating genes, pleiotropy, and sexual dimorphism at blood lipid loci through multi-ancestry meta-analysis.
- 2022
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Ingår i: Genome biology. - : Springer Science and Business Media LLC. - 1474-760X .- 1465-6906 .- 1474-7596. ; 23:1
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Tidskriftsartikel (refereegranskat)abstract
- Genetic variants within nearly 1000 loci are known to contribute to modulation of blood lipid levels. However, the biological pathways underlying these associations are frequently unknown, limiting understanding of these findings and hindering downstream translational efforts such as drug target discovery.To expand our understanding of the underlying biological pathways and mechanisms controlling blood lipid levels, we leverage a large multi-ancestry meta-analysis (N=1,654,960) of blood lipids to prioritize putative causal genes for 2286 lipid associations using six gene prediction approaches. Using phenome-wide association (PheWAS) scans, we identify relationships of genetically predicted lipid levels to other diseases and conditions. We confirm known pleiotropic associations with cardiovascular phenotypes and determine novel associations, notably with cholelithiasis risk. We perform sex-stratified GWAS meta-analysis of lipid levels and show that 3-5% of autosomal lipid-associated loci demonstrate sex-biased effects. Finally, we report 21 novel lipid loci identified on the X chromosome. Many of the sex-biased autosomal and X chromosome lipid loci show pleiotropic associations with sex hormones, emphasizing the role of hormone regulation in lipid metabolism.Taken together, our findings provide insights into the biological mechanisms through which associated variants lead to altered lipid levels and potentially cardiovascular disease risk.
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| 7. |
- Ramdas, S., et al.
(författare)
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A multi-layer functional genomic analysis to understand noncoding genetic variation in lipids
- 2022
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Ingår i: American Journal of Human Genetics. - : Elsevier BV. - 0002-9297 .- 1537-6605. ; 109:8, s. 1366-1387
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Tidskriftsartikel (refereegranskat)abstract
- A major challenge of genome-wide association studies (GWASs) is to translate phenotypic associations into biological insights. Here, we integrate a large GWAS on blood lipids involving 1.6 million individuals from five ancestries with a wide array of functional genomic datasets to discover regulatory mechanisms underlying lipid associations. We first prioritize lipid-associated genes with expression quantitative trait locus (eQTL) colocalizations and then add chromatin interaction data to narrow the search for functional genes. Polygenic enrichment analysis across 697 annotations from a host of tissues and cell types confirms the central role of the liver in lipid levels and highlights the selective enrichment of adipose-specific chromatin marks in high-density lipoprotein cholesterol and triglycerides. Overlapping transcription factor (TF) binding sites with lipid-associated loci identifies TFs relevant in lipid biology. In addition, we present an integrative framework to prioritize causal variants at GWAS loci, producing a comprehensive list of candidate causal genes and variants with multiple layers of functional evidence. We highlight two of the prioritized genes, CREBRF and RRBP1, which show convergent evidence across functional datasets supporting their roles in lipid biology.
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| 8. |
- Wang, Fei, et al.
(författare)
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Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level
- 2022
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Ingår i: Nature Communications. - : Springer Nature. - 2041-1723. ; 13:1
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Tidskriftsartikel (refereegranskat)abstract
- Pigs are valuable large animal models for biomedical and genetic research, but insights into the tissue- and cell-type-specific transcriptome and heterogeneity remain limited. By leveraging single-cell RNA sequencing, we generate a multiple-organ single-cell transcriptomic map containing over 200,000 pig cells from 20 tissues/organs. We comprehensively characterize the heterogeneity of cells in tissues and identify 234 cell clusters, representing 58 major cell types. In-depth integrative analysis of endothelial cells reveals a high degree of heterogeneity. We identify several functionally distinct endothelial cell phenotypes, including an endothelial to mesenchymal transition subtype in adipose tissues. Intercellular communication analysis predicts tissue- and cell type-specific crosstalk between endothelial cells and other cell types through the VEGF, PDGF, TGF-beta, and BMP pathways. Regulon analysis of single-cell transcriptome of microglia in pig and 12 other species further identifies MEF2C as an evolutionally conserved regulon in the microglia. Our work describes the landscape of single-cell transcriptomes within diverse pig organs and identifies the heterogeneity of endothelial cells and evolutionally conserved regulon in microglia.
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| 9. |
- Xue, Ru, et al.
(författare)
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Variations of methane fluxes and methane microbial community composition with soil depth in the riparian buffer zone of a sponge city park
- 2023
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Ingår i: Journal of Environmental Management. - : Elsevier. - 0301-4797 .- 1095-8630. ; 339
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Tidskriftsartikel (refereegranskat)abstract
- Riparian buffers benefit both natural and man-made ecosystems by preventing soil erosion, retaining soil nu-trients, and filtering pollutants. Nevertheless, the relationship between vertical methane fluxes, soil carbon, and methane microbial communities in riparian buffers remains unclear. This study examined vertical methane fluxes, soil carbon, and methane microbial communities in three different soil depths (0-5 cm, 5-10 cm, and 10-15 cm) within a riparian buffer of a Sponge City Park for one year. Structural equation model (SEM) results demonstrated that vertical methane fluxes varied with soil depths (lambda =-0.37) and were primarily regulated by methanogenic community structure (lambda = 0.78). Notably, mathematical regression results proposed that mcrA/ pmoA ratio (R2 = 0.8) and methanogenic alpha diversity/methanotrophic alpha diversity ratio (R2 = 0.8) could serve as valid predictors of vertical variation in methane fluxes in the riparian buffer of urban river. These findings suggest that vertical variation of methane fluxes in riparian buffer soils is mainly influenced by carbon inputs and methane microbial abundance and community diversity. The study's results quantitatively the relationship between methane fluxes in riparian buffer soils and abiotic and biotic factors in the vertical di-rection, therefore contributing to the further development of mathematical models of soil methane emissions.
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| 10. |
- Campbell, PJ, et al.
(författare)
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Pan-cancer analysis of whole genomes
- 2020
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 1476-4687 .- 0028-0836. ; 578:7793, s. 82-
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Tidskriftsartikel (refereegranskat)abstract
- Cancer is driven by genetic change, and the advent of massively parallel sequencing has enabled systematic documentation of this variation at the whole-genome scale1–3. Here we report the integrative analysis of 2,658 whole-cancer genomes and their matching normal tissues across 38 tumour types from the Pan-Cancer Analysis of Whole Genomes (PCAWG) Consortium of the International Cancer Genome Consortium (ICGC) and The Cancer Genome Atlas (TCGA). We describe the generation of the PCAWG resource, facilitated by international data sharing using compute clouds. On average, cancer genomes contained 4–5 driver mutations when combining coding and non-coding genomic elements; however, in around 5% of cases no drivers were identified, suggesting that cancer driver discovery is not yet complete. Chromothripsis, in which many clustered structural variants arise in a single catastrophic event, is frequently an early event in tumour evolution; in acral melanoma, for example, these events precede most somatic point mutations and affect several cancer-associated genes simultaneously. Cancers with abnormal telomere maintenance often originate from tissues with low replicative activity and show several mechanisms of preventing telomere attrition to critical levels. Common and rare germline variants affect patterns of somatic mutation, including point mutations, structural variants and somatic retrotransposition. A collection of papers from the PCAWG Consortium describes non-coding mutations that drive cancer beyond those in the TERT promoter4; identifies new signatures of mutational processes that cause base substitutions, small insertions and deletions and structural variation5,6; analyses timings and patterns of tumour evolution7; describes the diverse transcriptional consequences of somatic mutation on splicing, expression levels, fusion genes and promoter activity8,9; and evaluates a range of more-specialized features of cancer genomes8,10–18.
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| 11. |
- Huang, Shan, et al.
(författare)
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Dipyridamole enhances the anti-cancer ability of aspirin against colorectal cancer by inducing apoptosis in an unfolded protein response-dependent manner
- 2023
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Ingår i: Cellular Oncology. - : Springer Science and Business Media LLC. - 2211-3428 .- 2211-3436. ; 46:4, s. 953-967
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Tidskriftsartikel (refereegranskat)abstract
- PURPOSE: Available evidence indicates that dipyridamole enhances the anti-thrombotic effects of aspirin for the prevention of secondary strokes. Aspirin is a well-known non-steroid anti-inflammatory drug. This anti-inflammatory property has turned aspirin into a potential drug for inflammation-related cancers such as colorectal cancer (CRC). Here, we aimed to explore whether the anti-cancer effect of aspirin against CRC could be improved by combined administration with dipyridamole.METHODS: Population-based clinical data analysis was conducted to assess a possible therapeutic effect of combined dipyridamole and aspirin treatment in inhibiting CRC compared with either monotherapy. This therapeutic effect was further verified in different CRC mouse models, i.e. an orthotopic xenograft mouse model, an AOM/DSS mouse model, an Apc min/+ mouse model and a patient derived xenograft (PDX) mouse model. The in vitro effects of the drugs on CRC cells were tested using CCK8 and flow cytometry assays. RNA-Seq, Western blotting, qRT-PCR and flow cytometry were used to identify the underlying molecular mechanisms. RESULTS: We found that dipyridamole combined with aspirin had a better inhibitory effect on CRC than either monotherapy alone. The enhanced anti-cancer effect of the combined use of dipyridamole with aspirin was found to rely on the induction of an overwhelmed endoplasmic reticulum (ER) stress and subsequent pro-apoptotic unfolded protein response (UPR), which was different from the anti-platelet effect.CONCLUSIONS: Our data indicate that the anti-cancer effect of aspirin against CRC may be enhanced by combined administration with dipyridamole. In case further clinical studies confirm our findings, these may be repurposed as adjuvant agents.
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| 12. |
- Luo, Yifei, et al.
(författare)
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Technology Roadmap for Flexible Sensors
- 2023
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Ingår i: ACS Nano. - : American Chemical Society. - 1936-0851 .- 1936-086X. ; 17:6, s. 5211-5295
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Forskningsöversikt (refereegranskat)abstract
- Humans rely increasingly on sensors to address grand challenges and to improve quality of life in the era of digitalization and big data. For ubiquitous sensing, flexible sensors are developed to overcome the limitations of conventional rigid counterparts. Despite rapid advancement in bench-side research over the last decade, the market adoption of flexible sensors remains limited. To ease and to expedite their deployment, here, we identify bottlenecks hindering the maturation of flexible sensors and propose promising solutions. We first analyze challenges in achieving satisfactory sensing performance for real-world applications and then summarize issues in compatible sensor-biology interfaces, followed by brief discussions on powering and connecting sensor networks. Issues en route to commercialization and for sustainable growth of the sector are also analyzed, highlighting environmental concerns and emphasizing nontechnical issues such as business, regulatory, and ethical considerations. Additionally, we look at future intelligent flexible sensors. In proposing a comprehensive roadmap, we hope to steer research efforts towards common goals and to guide coordinated development strategies from disparate communities. Through such collaborative efforts, scientific breakthroughs can be made sooner and capitalized for the betterment of humanity.
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| 13. |
- Tian, Xue-Chan, et al.
(författare)
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Unique gene duplications and conserved microsynteny potentially associated with resistance to wood decay in the Lauraceae
- 2023
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Ingår i: Frontiers in Plant Science. - : Frontiers Media S.A.. - 1664-462X. ; 14
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Tidskriftsartikel (refereegranskat)abstract
- Wood decay resistance (WDR) is marking the value of wood utilization. Many trees of the Lauraceae have exceptional WDR, as evidenced by their use in ancient royal palace buildings in China. However, the genetics of WDR remain elusive. Here, through comparative genomics, we revealed the unique characteristics related to the high WDR in Lauraceae trees. We present a 1.27-Gb chromosome-level assembly for Lindera megaphylla (Lauraceae). Comparative genomics integrating major groups of angiosperm revealed Lauraceae species have extensively shared gene microsynteny associated with the biosynthesis of specialized metabolites such as isoquinoline alkaloids, flavonoid, lignins and terpenoid, which play significant roles in WDR. In Lauraceae genomes, tandem and proximal duplications (TD/PD) significantly expanded the coding space of key enzymes of biosynthesis pathways related to WDR, which may enhance the decay resistance of wood by increasing the accumulation of these compounds. Among Lauraceae species, genes of WDR-related biosynthesis pathways showed remarkable expansion by TD/PD and conveyed unique and conserved motifs in their promoter and protein sequences, suggesting conserved gene collinearity, gene expansion and gene regulation supporting the high WDR. Our study thus reveals genomic profiles related to biochemical transitions among major plant groups and the genomic basis of WDR in the Lauraceae.
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| 14. |
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| 15. |
- Zhou, Wei, et al.
(författare)
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Global Biobank Meta-analysis Initiative : Powering genetic discovery across human disease
- 2022
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Ingår i: Cell Genomics. - : Elsevier. - 2666-979X. ; 2:10
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Tidskriftsartikel (refereegranskat)abstract
- Biobanks facilitate genome-wide association studies (GWASs), which have mapped genomic loci across a range of human diseases and traits. However, most biobanks are primarily composed of individuals of European ancestry. We introduce the Global Biobank Meta-analysis Initiative (GBMI)-a collaborative network of 23 biobanks from 4 continents representing more than 2.2 million consented individuals with genetic data linked to electronic health records. GBMI meta-analyzes summary statistics from GWASs generated using harmonized genotypes and phenotypes from member biobanks for 14 exemplar diseases and endpoints. This strategy validates that GWASs conducted in diverse biobanks can be integrated despite heterogeneity in case definitions, recruitment strategies, and baseline characteristics. This collaborative effort improves GWAS power for diseases, benefits understudied diseases, and improves risk prediction while also enabling the nomination of disease genes and drug candidates by incorporating gene and protein expression data and providing insight into the underlying biology of human diseases and traits.
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| 16. |
- Chen, Xue-Lian, et al.
(författare)
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Polyphenolics from Syzygium brachythyrsum Inhibits Oxidized Low-Density Lipoprotein-Induced Macrophage-Derived Foam Cell Formation and Inflammation
- 2022
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Ingår i: Foods. - : MDPI. - 2304-8158. ; 11:21
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Tidskriftsartikel (refereegranskat)abstract
- Evidence suggests that the immunomodulatory property of polyphenols may also contribute to the reduction of cardiovascular risk. In the present study, we investigated the polyphenol extraction (PE) from Syzygium brachythyrsum, a functional food resource in south China, regarding the protective effect on inhibiting foam cell formation and the underlying molecular mechanism based on an ox-LDL-induced RAW264.7 macrophage model. The results of Oil Red O staining, Dil-ox-LDL fluorescent staining, and cholesterol efflux experiments showed that PE, and its two phenolics brachythol B (BB) and ethyl gallate (EG), significantly inhibited the foam cell formation, which may be associated with reducing the expression of SR-A1 and CD36 while increasing expression of SR-B1, ABCG1, and PPAR gamma. In addition, BB and EG also reduce the inflammatory response by down-regulating the expression of NF-kappa B and MAPK signal pathway proteins, thereby inhibiting the expression of inflammatory factors. Altogether, PE and its two components BB and EG attenuated foam cell formation and macrophage inflammation response.
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| 17. |
- Deng, Huan, et al.
(författare)
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Progress of selenium deficiency in the pathogenesis of arthropathies and selenium supplement for their treatment
- 2022
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Ingår i: Biological Trace Element Research. - : Springer Nature. - 0163-4984 .- 1559-0720. ; 200, s. 4238-4249
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Forskningsöversikt (refereegranskat)abstract
- Selenium, an essential trace element for human health, exerts an indispensable effect in maintaining physiological homeostasis and functions in the body. Selenium deficiency is associated with arthropathies, such as Kashin-Beck disease, rheumatoid arthritis, osteoarthritis, and osteoporosis. Selenium deficiency mainly affects the normal physiological state of bone and cartilage through oxidative stress reaction and immune reaction. This review aims to explore the role of selenium deficiency and its mechanisms existed in the pathogenesis of arthropathies. Meanwhile, this review also summarized various experiments to highlight the crucial functions of selenium in maintaining the homeostasis of bone and cartilage.
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| 18. |
- He, X. L., et al.
(författare)
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Fast generation of Schrödinger cat states using a Kerr-tunable superconducting resonator
- 2023
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Ingår i: Nature Communications. - 2041-1723 .- 2041-1723. ; 14:1
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Tidskriftsartikel (refereegranskat)abstract
- Schrödinger cat states, quantum superpositions of macroscopically distinct classical states, are an important resource for quantum communication, quantum metrology and quantum computation. Especially, cat states in a phase space protected against phase-flip errors can be used as a logical qubit. However, cat states, normally generated in three-dimensional cavities and/or strong multi-photon drives, are facing the challenges of scalability and controllability. Here, we present a strategy to generate and preserve cat states in a coplanar superconducting circuit by the fast modulation of Kerr nonlinearity. At the Kerr-free work point, our cat states are passively preserved due to the vanishing Kerr effect. We are able to prepare a 2-component cat state in our chip-based device with a fidelity reaching 89.1% under a 96 ns gate time. Our scheme shows an excellent route to constructing a chip-based bosonic quantum processor.
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| 19. |
- Huang, D., et al.
(författare)
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Electronic structure, defect properties, and optimization of the band gap of the earth-abundant and low-toxicity photovoltaic absorber Cu3SbS4
- 2022
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Ingår i: Physical Chemistry, Chemical Physics - PCCP. - : Royal Society of Chemistry (RSC). - 1463-9076 .- 1463-9084. ; 24:41, s. 25258-25269
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Tidskriftsartikel (refereegranskat)abstract
- Searching for an earth-abundant and environment-friendly absorber for thin-film solar cells that provides similar power conversion efficiency to CdTe and Cu(In,Ga)Se2 is of great importance for large-scale applications. Success would change the world's solar energy supply to an even more sustainable material resource. In this paper, we have studied by first-principles calculations the electronic structure and defect properties of the promising absorber Cu3SbS4. Its electronic properties, like direct band gap, high absorption coefficient, and light carrier effective masses, satisfy the requirements for an absorber except for its somewhat too small band gap energy. Sulfur and copper vacancies are easily formed defects in Cu3SbS4, where the S vacancy shrinks the band gap and degrades the material. This probably explains the experimental findings of a rather poor device performance. The suitable preparation conditions for Cu3SbS4 as an absorber are anticipated to be Cu-poor, Sb-moderate, and S-rich conditions. Herein, isovalent element alloying is demonstrated to be an effective way to increase the gap energy and thereby improve the material properties.
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| 20. |
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| 21. |
- Huang, Hongyun, et al.
(författare)
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The 2022 yearbook of neurorestoratology
- 2023
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Ingår i: JOURNAL OF NEURORESTORATOLOGY. - : Tsinghua University Press. - 2324-2426. ; 11:2
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Forskningsöversikt (refereegranskat)abstract
- There was much progress in the field of Neurorestoratology in the year of 2022. It included highlighting advances in understanding the pathogenesis of neurological diseases, neurorestorative mechanisms, and clinical treatments as compiled in the 2022 yearbook of Neurorestoratology. There is still controversy about whether amyloid b-protein and tau protein deposition are the reasons for or the results of Alzheimer's disease (AD) pathology. The fabricated images in important key articles that speculated on the reasons for AD pathogenesis were found. Cholinergic deficiency and decrease or loss in strength of glutamatergic synapse, limited or failing bidirectional cholinergic upregulation in early cognitive impairment, or progressive posterior-to-anterior cortical cholinergic denervation could result in the appearance of AD. Exploration of neurorestorative mechanisms were found in more detail ways in neuromodulation, immunomodulation, neurogenesis, neural network or circuitry reconstruction, neuroprotection, nervous structural repair, and neuroplasticity. Several kinds of cell therapies for neurological diseases showed neurorestorative effects in open-label and/or non-randomized clinical studies or trials. However, mesenchymal stromal cells and mononuclear cells did not demonstrate neurorestorative effects or improve the quality of life for patients with neurodegenerative diseases or neurotrauma including stroke, spinal cord injury (SCI), and amyotrophic lateral sclerosis in randomized, double-blind, placebo-controlled clinical trials (RDPCTs). Clinical treatments through neurostimulation/neuromodulati on and the brain-computer/ machine interface yielded positive results in AD, Parkinson's disease, stroke, SCI, cerebral palsy, and other diseases in RDPCTs. Neurorestorative surgery, pharmaceutical neurorestorative therapy and other interventions have demonstrated neurorestorative effects for various considered incurable neurological diseases in RDPCTs. Thus, this year, additional guidelines, assessment scales, and standards were set up or revised. These included guidelines of clinical neurorestorative treatments for brain trauma (2022 China version), clinical cell therapy guidelines for neurorestoration (IANR/CANR 2022), SCI or dysfunction quality of life rating scale (SCIDQLRS) (IANR 2022 version). Neurorestorative effects of varying therapeutic stra-tegies with higher standards of evidence-based medicine are now benefiting patients with currently incurable neurological diseases. Hopefully some of them may become routine therapeutic interventions for patients with these diseases in the near future.
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| 22. |
- Huang, Yi Shu, et al.
(författare)
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Pharmacological modulation of T cell immunity results in long-term remission of autoimmune arthritis
- 2021
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490. ; 118:19
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Tidskriftsartikel (refereegranskat)abstract
- Chronic inflammatory diseases like rheumatoid arthritis are characterized by a deficit in fully functional regulatory T cells. DNA-methylation inhibitors have previously been shown to promote regulatory T cell responses and, in the present study, we evaluated their potential to ameliorate chronic and acute animal models of rheumatoid arthritis. Of the drugs tested, decitabine was the most effective, producing a sustained therapeutic effect that was dependent on indoleamine 2,3-dioxygenase (IDO) and was associated with expansion of induced regulatory T cells, particularly at the site of disease activity. Treatment with decitabine also caused apoptosis of Th1 and Th17 cells in active arthritis in a highly selective manner. The molecular basis for this selectivity was shown to be ENT1, a nucleoside transporter, which facilitates intracellular entry of the drug and is up-regulated on effector T cells during active arthritis. It was further shown that short-term treatment with decitabine resulted in the generation of a population of regulatory T cells that were able to suppress arthritis upon adoptive transfer. In summary, a therapeutic approach using an approved drug is described that treats active inflammatory disease effectively and generates robust regulatory T cells with the IDO-dependent capacity to maintain remission.
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| 23. |
- Jiang, Bing-Xin, et al.
(författare)
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Fabrication and bonding of In bumps on Micro-LED with 8 μ m pixel pitch
- 2024
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Ingår i: ENGINEERING RESEARCH EXPRESS. - 2631-8695. ; 6:2
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Tidskriftsartikel (refereegranskat)abstract
- Indium (In) is currently used to fabricate metal bumps on micro-light-emitting diode (Micro-LED) chips due to its excellent physical properties. However, as Micro-LED pixel size and pitch decrease, achieving high-quality In bumps on densely packed Micro-LED chips often presents more challenges. This paper describes the process of fabricating In bumps on micro-LEDs using thermal evaporation, highlighting an issue where In tends to grow laterally within the photoresist pattern, ultimately blocking the pattern and resulting in undersized and poorly dense In bumps on the Micro-LED chip. To address this issue, we conducted numerous experiments to study the height variation of In bumps within a range of photoresist aperture sizes (3 mu m -7 mu m) under two different resist thickness conditions (3.8 mu m and 4.8 mu m). The results showed that the resist thickness had a certain effect on the height of In bumps on the Micro-LED chip electrodes. Moreover, we found that, with the photoresist pattern size increasing under constant resist thickness conditions, the height and quality of the bumps significantly improved. Based on this finding, we rationalized the adjustment of the photoresist pattern size within a limited emission platform range to compensate for the height difference of In bumps caused by different resist thicknesses between the cathode and anode regions. Consequently, well-shaped and dense In bumps with a maximum height of up to 4.4 mu m were fabricated on 8 mu m pitch Micro-LED chips. Afterwards, we bonded the Micro-LED chip with indium bumps to the CMOS chip, and we found that we could successfully control the CMOS chip to drive the Micro-LED chip to display specific characters through the Flexible Printed Circuit (FPC). This work is of significant importance for the fabrication of In bumps on Micro-LED chips with pitches below 10 mu m and subsequent bonding processes.
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| 24. |
- Jiao, Zhejing, et al.
(författare)
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InAs triangular quantum wells grown on InP/SiO 2 /Si heterogeneous substrate for mid-infrared emission
- 2021
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Ingår i: Materials Science in Semiconductor Processing. - : Elsevier BV. - 1369-8001. ; 136
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Tidskriftsartikel (refereegranskat)abstract
- The properties of InAs/In0.53Ga0.37As triangular quantum wells (QWs) grown on an InP/SiO2/Si integrated substrate by ion-slicing technology are investigated. The material structure and growth quality are characterized by the X-ray diffraction (XRD) and transmission electron microscope measurements. The photoluminescence (PL) spectra at various temperatures are also analyzed. The PL peak wavelengths red-shift from 1.94 to 2.13 μm with the increase of temperature from 12.4 to 300 K. The experimental results of the QWs on InP/SiO2/Si substrate are found to be comparable with the performance of the same QWs grown on an InP substrate. The results are promising for future integration of Si with InP-based optical devices for the applications of light emission in mid-infrared wavelength range.
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| 25. |
- Ju, Zhiping, et al.
(författare)
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First-principles prediction on Ag3SbS4 as a photovoltaic absorber
- 2023
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Ingår i: Journal of Physics and Chemistry of Solids. - : Elsevier BV. - 0022-3697 .- 1879-2553. ; 183
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Tidskriftsartikel (refereegranskat)abstract
- Generally, tetrahedrally coordinated Ag-based chalcogenides have wider band gaps than their Cu-based counterparts. Recent studies have suggested Cu3SbS4 as the absorber in low-cost and low-toxicity photovoltaic (PV), however its band gap is ∼0.5 eV smaller than the ideal value of ∼1.3 eV. In this work, we investigate Ag3SbS4 by first-principles means, since one can anticipate improved optical properties for this compound. The results indeed demonstrate that enargite Ag3SbS4 is a direct-gap semiconductor with a band gap of 1.38 eV, thus optimal for single-junction solar cells. Furthermore, its carrier effective masses, optical coefficients and spectroscopic limited maximum efficiency are comparable to well-established PV compounds. The compound exhibits also thermodynamical and dynamical stability. Hence, based on the present theoretical study we predict that Ag3SbS4 could be a candidate for absorber in high-efficient thin-film PVs.
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| 26. |
- Köksal, Elif Senem, et al.
(författare)
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Rapid Growth and Fusion of Protocells in Surface-Adhered Membrane Networks
- 2020
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Ingår i: Small. - : Wiley. - 1613-6810 .- 1613-6829. ; 16:38
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Tidskriftsartikel (refereegranskat)abstract
- Elevated temperatures might have promoted the nucleation, growth, and replication of protocells on the early Earth. Recent reports have shown evidence that moderately high temperatures not only permit protocell assembly at the origin of life, but can have actively supported it. Here, the fast nucleation and growth of vesicular compartments from autonomously formed lipid networks on solid surfaces, induced by a moderate increase in temperature, are shown. Branches of the networks, initially consisting of self-assembled interconnected nanotubes, rapidly swell into microcompartments which can spontaneously encapsulate RNA fragments. The increase in temperature further causes fusion of adjacent network-connected compartments, resulting in the redistribution of the RNA. The experimental observations and the mathematical model indicate that the presence of nanotubular interconnections between protocells facilitates the fusion process.
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| 27. |
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| 28. |
- Li, Danqin, et al.
(författare)
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n-Doping of photoactive layer in binary organic solar cells realizes over 18.3% efficiency
- 2022
-
Ingår i: Nano Energy. - : ELSEVIER. - 2211-2855 .- 2211-3282. ; 96
-
Tidskriftsartikel (refereegranskat)abstract
- Electronic doping of conjugated semiconductor plays a critical role in the fabrication of high efficiency organic optoelectronic devices. Here, we report an organic solar cell (OSC) by doping n-type DMBI-BDZC into one host binary bulk heterojunction (BHJ) photoactive layer comprised of a polymer donor PM6 and a nonfullerene acceptor Y6. The resulting champion device yields a significantly improved power conversion efficiency from 17.17% to 18.33% with an impressive fill factor of 80.20%. It is found that the electrically doped photoactive layer exhibits enhanced and balanced charge carrier mobilities, more effective exciton dissociation, longer carrier lifetime, and suppressed charge recombination with smaller energy loss. The dopant molecule DMBIBDZC also act as a surface morphology modifier of the photoactive layer with enhanced charge transport. This work demonstrates that manipulation of charge transport via adding a low concentration dopant into photoactive layer is a promising approach for further improvement of BHJ OSC performance.
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| 29. |
- Liang, Pu-Lin, et al.
(författare)
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Three polymethoxyflavones from the peel of Citrus reticulata "Chachi" inhibits oxidized low-density lipoprotein-induced macrophage-derived foam cell formation
- 2022
-
Ingår i: Frontiers in Cardiovascular Medicine. - : Frontiers Media S.A.. - 2297-055X. ; 9
-
Tidskriftsartikel (refereegranskat)abstract
- Foam cell formation is the hallmark of the development and progression of atherosclerosis. The aim of this study was to investigate the regulatory effects of three polymethoxyflavones (PMFs), namely, tangeretin (TAN), 5,6,7,3 ',4 ',5 '-hexamethoxyflavone (HxMF), and 3,5,6,7,8,3 ',4 '-heptamethoxyflavone (HpMF) on macrophage-derived foam cell formation and to further explore the molecular mechanisms. The RAW264.7 macrophage-derived foam cell model was successfully induced by oxidized low-density lipoprotein (ox-LDL) (80 mu g/ml). It showed that TAN, HxMF, and HpMF alleviated ox-LDL-induced NO release while also inhibiting the expression of IL-1 beta, IL-6, and TNF-alpha in RAW264.7 cells. Uptake of excess ox-LDL was inhibited by TAN, HxMF, and HpMF, resulting in the reduction of its foam cell formation. Moreover, TAN, HxMF, and HpMF promoted HDL-mediated cholesterol efflux. Western blot experiment showed that TAN, HxMF, and HpMF inhibited the expression of scavenger receptor class A type I (SRA1) and cluster of differentiation 36 (CD36), while upregulating peroxisome proliferator-activated receptor gamma (PPAR gamma), liver X receptor alpha (LXR alpha), phospholipid ATP-binding cassette transporter G1 (ABCG1), and scavenger receptor class B type I (SRB1) expression. Together, our findings suggested that PMFs inhibited foam cell formation might inhibit lipid uptake via downregulating SRA1/CD36 expression and promote cholesterol efflux from foam cells via upregulating PPAR gamma/LXR alpha/ABCG1/SRB1 expression. This antiatherosclerotic activity is expected to provide new insights into the development of healthcare uses for PMFs.
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| 30. |
- Lin, Qi, et al.
(författare)
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Characterization of lacustrine harmful algal blooms using multiple biomarkers : historical processes, driving synergy, and ecological shifts
- 2023
-
Ingår i: Water Research. - : Elsevier. - 0043-1354 .- 1879-2448. ; 235
-
Tidskriftsartikel (refereegranskat)abstract
- Harmful algal blooms (HABs) producing toxic metabolites are increasingly threatening environmental and human health worldwide. Unfortunately, long-term process and mechanism triggering HABs remain largely unclear due to the scarcity of temporal monitoring. Retrospective analysis of sedimentary biomarkers using up-to-date chromatography and mass spectrometry techniques provide a potential means to reconstruct the past occurrence of HABs. By combining aliphatic hydrocarbons, photosynthetic pigments, and cyanotoxins, we quantified herein century-long changes in abundance, composition, and variability of phototrophs, particularly toxigenic algal blooms, in China's third largest freshwater Lake Taihu. Our multi-proxy limnological reconstruction revealed an abrupt ecological shift in the 1980s characterized by elevated primary production, Microcystis-dominated cyanobacterial blooms, and exponential microcystin production, in response to nutrient enrichment, climate change, and trophic cascades. The empirical results from ordination analysis and generalized additive models support climate warming and eutrophication synergy through nutrient recycling and their feedback through buoyant cyanobacterial proliferation, which sustain bloom-forming potential and further promote the occurrence of increasingly-toxic cyanotoxins (e.g., microcystin-LR) in Lake Taihu. Moreover, temporal variability of the lake ecosystem quantified using variance and rate of change metrics rose continuously after state change, indicating increased ecological vulnerability and declined resilience following blooms and warming. With the persistent legacy effects of lake eutrophication, nutrient reduction efforts mitigating toxic HABs probably be overwhelmed by climate change effects, emphasizing the need for more aggressive and integrated environmental strategies.
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| 31. |
- Ma, Lin Lu, et al.
(författare)
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Coronavirus Disease 2019 Related Clinical Studies : A Cross-Sectional Analysis
- 2020
-
Ingår i: Frontiers in Pharmacology. - : Frontiers Media SA. - 1663-9812. ; 11
-
Tidskriftsartikel (refereegranskat)abstract
- Objective: The quality and rationality of many recently registered clinical studies related to coronavirus disease 2019 (COVID-19) needs to be assessed. Hence, this study aims to evaluate the current status of COVID-19 related registered clinical trial. Methods: We did an electronic search of COVID-19 related clinical studies registered between December 1, 2019 and February 21, 2020 (updated to May 28, 2020) from the ClinicalTrials.gov, and collected registration information, study details, recruitment status, characteristics of the subjects, and relevant information about the trial implementation process. Results: A total of 1,706 studies were included 10.0% of which (n=171) were from France, 943 (55.3%) used an interventional design, and 600 (35.2%) used an observational design. Most of studies (73.6%) aimed to recruit fewer than 500 people. Interferon was the main prevention program, and antiviral drugs were the main treatment program. Hydroxychloroquine and chloroquine (230/943, 24.4%) were widely studied. Some registered clinical trials are incomplete in content, and 37.4% of the 1,706 studies may have had insufficient sample size. Conclusion: The quality of COVID-19 related studies needs to be improved by strengthening the registration process and improving the quality of clinical study protocols so that these clinical studies can provide high-quality clinical evidence related to COVID-19.
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| 32. |
- Nguyen, Thao Duy, et al.
(författare)
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Monobutyrin and monovalerin affect brain short-chain fatty acid profiles and tight-junction protein expression in apoe-knockout rats fed high-fat diets
- 2020
-
Ingår i: Nutrients. - : MDPI AG. - 2072-6643. ; 12:4
-
Tidskriftsartikel (refereegranskat)abstract
- Monobutyrin (MB) and monovalerin (MV), esters of short-chain fatty acids (SCFAs), have previously been shown to reduce liver cholesterol and inflammation in conventional rats fed high-fat diets. This study explored the potential effects of MB and MV in hypercholesterolemic apolipoprotein E-knockout (ApoE-/-) rats. ApoE-/-rats were fed three high-fat (HF) diets, pure or supplemented with MB or MV (1%), for 5 weeks. One group of conventional rats (C) was also fed the pure high-fat diet and another group of ApoE-/-rats a low-fat (LF) diet. Blood and liver lipids, urinary lactulose/mannitol, SCFAs (blood and brain), tight junction proteins (small intestine and brain), and inflammation-related markers (blood, brain, and liver) were analyzed. MV supplementation elevated serum high-density lipoprotein (HDL) cholesterol and valeric acid concentration (p < 0.05), while the amounts of isovaleric acid in the brain were reduced (p < 0.05). MB increased butyric acid amounts in the brain, while the plasma concentration of interleukin 10 (IL-10) was lowered (p < 0.05). Both MV and MB upregulated the expression of occludin and zonula occludens-1 (ZO-1) in the brain (p < 0.05). Supplementation of MB or MV affected HDL cholesterol, the expression of tight junction proteins, and SCFA profiles. MB and MV may therefore be promising supplements to attenuate lipid metabolic disorders caused by high-fat intake and genetic deficiency.
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| 33. |
- Pang, Xiaodan, Dr., et al.
(författare)
-
200 Gbps/lane IM/DD Technologies for Short Reach Optical Interconnects
- 2020
-
Ingår i: Journal of Lightwave Technology. - 0733-8724 .- 1558-2213. ; 38:2
-
Tidskriftsartikel (refereegranskat)abstract
- Client-side optics are facing an ever-increasing upgrading pace, driven by upcoming 5G related services and datacenter applications. The demand for a single lane data rate is soon approaching 200 Gbps. To meet such high-speed requirements, all segments of traditional intensity modulation direct detection (IM/DD) technologies are being challenged. The characteristics of electrical and optoelectronic components, and the performance of modulation, coding and digital signal processing (DSP) techniques are being stretched to their limits. In this context, we witnessed technological breakthroughs in several aspects, including development of broadband devices, novel modulation formats and coding, and high-performance DSP algorithms for the past few years. A great momentum has been accumulated to overcome the aforementioned challenges. In this paper, we focus on IM/DD transmissions, and provide an overview of recent research and development efforts on key enabling technologies for 200 Gbps per lane and beyond. Our recent demonstrations of 200 Gbps short-reach transmissions with 4-level pulse amplitude modulation (PAM) and discrete multitone signals are also presented as examples to show the system requirements in terms of device characteristics and DSP performance. Apart from digital coherent technologies and advanced direct detection systems, such as Stokes-vector and Kramers-Kronig schemes, we expect high-speed IM/DD systems will remain advantageous in terms of system cost, power consumption and footprint for short reach applications in the short- to mid- term perspective.
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| 34. |
- Pang, Xiaodan, Dr., et al.
(författare)
-
200 Gbps & x002F;Lane IM & x002F;DD Technologies for Short Reach Optical Interconnects
- 2020
-
Ingår i: Journal of Lightwave Technology. - : Institute of Electrical and Electronics Engineers (IEEE). - 0733-8724 .- 1558-2213. ; 38:2, s. 492-503
-
Tidskriftsartikel (refereegranskat)abstract
- Client-side optics are facing an ever-increasing upgrading pace, driven by upcoming 5G related services and datacenter applications. The demand for a single lane data rate is soon approaching 200 Gbps. To meet such high-speed requirement, all segments of traditional intensity modulation direct detection (IM & x002F;DD) technologies are being challenged. The characteristics of electrical and optoelectronic components and the performance of modulation, coding, and digital signal processing (DSP) techniques are being stretched to their limits. In this context, we witnessed technological breakthroughs in several aspects, including development of broadband devices, novel modulation formats and coding, and high-performance DSP algorithms for the past few years. A great momentum has been accumulated to overcome the aforementioned challenges. In this article, we focus on IM & x002F;DD transmissions, and provide an overview of recent research and development efforts on key enabling technologies for 200 Gbps per lane and beyond. Our recent demonstrations of 200 Gbps short-reach transmissions with 4-level pulse amplitude modulation (PAM) and discrete multitone signals are also presented as examples to show the system requirements in terms of device characteristics and DSP performance. Apart from digital coherent technologies and advanced direct detection systems, such as Stokes-vector and Kramers-Kronig schemes, we expect high-speed IM & x002F;DD systems will remain advantageous in terms of system cost, power consumption, and footprint for short reach applications in the short- to mid- term perspective.
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| 35. |
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| 36. |
- Shao, Xue-Feng, et al.
(författare)
-
Hydroxyl group functionalized graphene oxide nanosheets as additive for improved erythritol latent heat storage performance : A comprehensive evaluation on the benefits and challenges
- 2020
-
Ingår i: Solar Energy Materials and Solar Cells. - : Elsevier BV. - 0927-0248 .- 1879-3398. ; 215
-
Tidskriftsartikel (refereegranskat)abstract
- Graphene oxide (GO) nanosheets were employed as the additive to make composites of erythritol, a promising medium-temperature PCM candidate. GO nanosheets modified with hydroxyl groups were applied to improve the dispersion stability of the composites. A systematic characterization on the latent heat storage performance was performed for both pure and composite erythritol, in order to identify the benefits and challenges of the composites. It was found that the thermal conductivity is increased by nearly twice and the degree of supercooling was lowered from ~64 °C to ~48 °C at the loading of 1.0 wt% GO nanosheets (the maximum loading tested). The addition of GO nanosheets also leads to an increase of the retrievable latent heat during crystallization, from ~187 kJ/kg to ~225 kJ/kg at the same loading, by increasing the crystallinity. However, the introduction of GO nanosheets can also lead to a rise in the dynamic viscosity of erythritol. As a result, the crystallization rate is slowed down and accordingly, the duration of crystallization becomes 62% longer when the loading reaches 1.0 wt%. In addition, favorable dispersion stability of the erythritol composites is observed, and their melting point (~117 °C) remains almost unchanged during 50 melting-crystallization cycles. Functionalized GO nanosheets have been shown to be an efficient additive for improving the performance of erythritol, but a trade-off analysis on the loading would be required to achieve the best overall performance.
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| 37. |
- Shao, Xue-Feng, et al.
(författare)
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Polyvinylpyrrolidone (PVP)-enabled significant suppression of supercooling of erythritol for medium-temperature thermal energy storage
- 2022
-
Ingår i: Journal of Energy Storage. - : Elsevier BV. - 2352-152X .- 2352-1538. ; 46, s. 103915-
-
Tidskriftsartikel (refereegranskat)abstract
- The supercooling effect is deemed to be a crucial issue for thermal energy storage using phase change materials (PCMs). The exploration of promising additives plays a decisive role in effective suppression efforts for suppressing the supercooling effect of a PCM. The present work proposed a potential additive, polyvinylpyrrolidone (PVP), to reduce the supercooling of erythritol, which is the most promising polyol PCM candidate for medium temperature range. PVP with various loadings was dispersed in erythritol to make composites for the proof-of-concept tests. It was shown that the degree of supercooling of erythritol can be reduced significantly from over 64 ? to about 21 ? in the presence of only 1.0 wt.% PVP. Along with the mitigated supercooling effect, the addition of PVP also leads to an increase of the retrievable latent heat during crystallization, from ~187 J/g to ~224 J/g at the same minute PVP loading of 1.0 wt.%, by increasing the crystallinity of erythritol. The PVP-loaded erythritol composites exhibit little sacrifice in latent heat of fusion, i.e., only ~15% loss when the PVP loading reaches 6.0 wt.%. In addition, multiple tests confirmed that PVP can be dissolved in erythritol, thus desirable compatibility was obtained and the composites would have long-term reliability. This proposed additive enables an efficient and cost-effective way for improving the crystallization behaviors of erythritol (and other polyol PCMs) towards real-world applications.
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| 38. |
- Shi, Xue, et al.
(författare)
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A strong, biodegradable, and recyclable all-lignocellulose fabricated triboelectric nanogenerator for self-powered disposable medical monitoring
- 2023
-
Ingår i: Journal of Materials Chemistry A. - 2050-7488 .- 2050-7496. ; 11:22, s. 11730-11739
-
Tidskriftsartikel (refereegranskat)abstract
- The growing demand for fast, reliable, and accessible information in the vastly connected world makes disposable sensors increasingly important. However, reducing their costs, environmental impact, and usability remains challenging. Here, we report a low-cost, biodegradable, and recyclable all-lignocellulosic triboelectric nanogenerator (AL-TENG) for self-powered disposable medical monitoring. Based on a facile in situ lignin regeneration & chemical crosslinking modification strategy, a high-performance lignocellulosic bioplastic is synthesized from resource-abundant and renewable biomass for fabricating the AL-TENG. The whole device has a low environmental impact as it can be easily recycled and biodegraded at its end-of-life. Furthermore, a self-powered smart ward system and a self-powered contactless medical monitoring system are developed to improve the convenience for patients and reduce the risk of mutual infection. This work can expand the application of self-powered systems to disposable medical sensing, which may greatly promote the development of intelligent wards and disposable electronics.
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| 39. |
- Spustova, Karolina, et al.
(författare)
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Manipulation of Lipid Membranes with Thermal Stimuli
- 2022
-
Ingår i: Methods in Molecular Biology. - New York, NY : Springer US. - 1940-6029 .- 1064-3745. ; , s. 209-225
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- We describe a protocol for the assembly and application of infrared (IR-B) laser-based set-ups to be used for localized heating of solid-supported planar and vesicular lipid membrane assemblies.
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| 40. |
- Tan, Xiaoqin, et al.
(författare)
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Band gap tailoring in a low toxicity and low-cost solar cell absorber Cu3SbS4 through Na alloying : A first-principles study
- 2023
-
Ingår i: Journal of Crystal Growth. - : Elsevier BV. - 0022-0248 .- 1873-5002. ; 607
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Tidskriftsartikel (refereegranskat)abstract
- High power conversion efficiency, high stability, low cost, and environmentally friendly manufacturing are the main requirements for a commercializable photovoltaic device. Cu3SbS4 is an eco-friendly and earth-abundant compound that is studied as a potential solar cell absorber. However, its band gap is smaller than the ideal value. In this work, the possibility to modulate and improve the band gap by sodium alloying has been investigated by means of the first-principles density functional theory with the HSE06 hybrid functional. Our results demonstrate that the Cu3-xNaxSbS4 alloy with a high alloying concentration should be possible to realize, and that the Na incorporation widens the gap. An alloy concentration of x approximate to 0.64 yields the desired gap for a solar light absorber, which can then lead to a more efficient solar cell.
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| 41. |
- Tang, Hu, et al.
(författare)
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Boron-Rich Molybdenum Boride with Unusual Short-Range Vacancy Ordering, Anisotropic Hardness, and Superconductivity
- 2020
-
Ingår i: Chemistry of Materials. - : AMER CHEMICAL SOC. - 0897-4756 .- 1520-5002. ; 32:1, s. 459-467
-
Tidskriftsartikel (refereegranskat)abstract
- Determination of the structures of materials involving more light elements such as boron-rich compounds is challenging and technically important in understanding their varied compositions and superior functionalities. Here we resolve the long-standing uncertainties in structure and composition about the highest boride (termed MoB4, Mo1-xB3, or MoB3) through the rapid formation of large sized boron-rich molybdenum boride under pressure. Using high-quality single-crystal X-ray diffraction analysis and aberration-corrected scanning transmission electron microscopy, we reveal that boron-rich molybdenum boride with a composition of Mo0.757B3 exhibits P6(3)/mmc symmetry with a partial occupancy of 0.514 in 211 Mo sites (Mol), and direct observations reveal the short-range ordering of cation vacancies in (010) crystal planes. Large anisotropic Young's moduli and Vickers hardness are seen for Mo0.757B3, which may be attributed by its two-dimensional boron distributions. Mo0.757B3 is also found to be superconducting with a transition temperature (T-c) of 2.4 K, which was confirmed by measurements of resistivity and magnetic susceptibility. Theoretical calculations suggest that the partial occupancy of Mo atoms plays a crucial role in the emergence of superconductivity.
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| 42. |
- Wan, Lu Ming, et al.
(författare)
-
Heparanase Facilitates PMA-Induced Megakaryocytic Differentiation in K562 Cells via Interleukin 6/STAT3 Pathway
- 2020
-
Ingår i: Thrombosis and Haemostasis. - : GEORG THIEME VERLAG KG. - 0340-6245 .- 2567-689X. ; 120:4, s. 647-657
-
Tidskriftsartikel (refereegranskat)abstract
- Heparanase (HPSE) is an endo-beta-D-glucuronidase that cleaves heparan sulfate and hence participates in remodeling of the extracellular matrix, leading to release of cytokines that are immobilized by binding to heparan sulfate proteoglycans (HSPGs), and consequently activating signaling pathways. This function of HPSE is correlated to its expression level that is normally very low in majority of the tissues. Exceptionally, human platelets express high level of HPSE, suggesting a unique physiological role in this cell. Using K562 cell line, we found a progressive increase of HPSE during the megakaryocytic differentiation. Analysis of a series of megakaryocytic differentiation-related heparin-binding proteins (HBPs) in the cell culture medium revealed an exclusive positive correlation between the level of interleukin 6 (IL-6) and HPSE expression. IL-6 modulated megakaryocytic differentiation through activation of STAT3. Further, we demonstrated that overexpression of HPSE potentiates megakaryocytic differentiation, whereas elimination of HPSE led to a delayed differentiation. This function of HPSE is associated with its activity, as overexpression of inactive HPSE had no effect on IL-6 production and megakaryocytic differentiation. The role of HPSE is further supported by the observation in an umbilical cord blood CD34+ cells megakaryocytic differentiation model. Our data propose a novel role for HPSE in platelets production by a HPSE/IL-6/STAT3 positive feedback loop that specifically regulates megakaryocytes maturation.
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| 43. |
- Wang, Zijun, et al.
(författare)
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Quantifying the Spatiotemporal Changes in Evapotranspiration and Its Components Driven by Vegetation Greening and Climate Change in the Northern Foot of Yinshan Mountain
- 2024
-
Ingår i: Remote Sensing. - 2072-4292. ; 16:2
-
Tidskriftsartikel (refereegranskat)abstract
- Evapotranspiration (E), a pivotal phenomenon inherent to hydrological and thermal dynamics, assumes a position of utmost importance within the intricate framework of the water–energy nexus. However, the quantitative study of E on a large scale for the “Grain for Green” projects under the backdrop of climate change is still lacking. Consequently, this study examined the interannual variations and spatial distribution patterns of E, transpiration (Et), and soil evaporation (Eb) in the Northern Foot of Yinshan Mountain (NFYM) between 2000 and 2020 and quantified the contributions of climate change and vegetation greening to the changes in E, Et, and Eb. Results showed that E (2.47 mm/a, p < 0.01), Et (1.30 mm/a, p < 0.01), and Eb (1.06 mm/a, p < 0.01) all exhibited a significant increasing trend during 2000–2020. Notably, vegetation greening emerged as the predominant impetus underpinning the augmentation of both E and Eb, augmenting their rates by 0.49 mm/a and 0.57 mm/a, respectively. In terms of Et, meteorological factors emerged as the primary catalysts, with temperature (Temp) assuming a predominant role by augmenting Et at a rate of 0.35 mm/a. Temp, Precipitation (Pre), and leaf area index (LAI) collectively dominated the proportional distribution of E, accounting for shares of 32.75%, 28.43%, and 25.01%, respectively. Within the spectrum of predominant drivers influencing Et, Temp exerted the most substantial influence, commanding the largest proportion at 33.83%. For Eb, the preeminent determinants were recognized as LAI and Temp, collectively constituting a substantial portion of the study area, accounting for 32.10% and 29.50%, respectively. The LAI exerted a pronounced direct influence on the Et, with no significant effects on E and bare Eb. Wind speed (WS) had a substantial direct impact on both E and Et. Pre exhibited a strong direct influence on E, Et, and Eb. Relative humidity (RH) significantly affected E directly. Temp primarily influenced Eb indirectly through radiation (Rad). Rad exerted a significant direct inhibitory effect on Eb. These findings significantly advanced our mechanistic understanding of how E and its components in the NFYM respond to climate change and vegetation greening, thus providing a robust basis for formulating strategies related to regional ecological conservation and water resources management, as well as supplying theoretical underpinnings for constructing sustainable vegetation restoration strategies involving water resources in the region.
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| 44. |
- Xu, Yingting, et al.
(författare)
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3-Pentanol glycosides from root nodules of the actinorhizal plant Alnus cremastogyne
- 2023
-
Ingår i: Phytochemistry. - : Elsevier BV. - 0031-9422 .- 1873-3700. ; 207
-
Tidskriftsartikel (refereegranskat)abstract
- Alnus cremastogyne Burkill (Betulaceae), an actinorhizal plant, can enter a mutualistic symbiosis with Frankia species that leads to the formation of nitrogen fixing root nodules. Some primary metabolites (carbohydrates, dicarboxylic acids, amino acids, citrulline and amides) involved in carbon and nitrogen metabolism in actinorhizal nodules have been identified, while specialized metabolites in A. cremastogyne root nodules are yet to be characterized. In this study, we isolated and identified three undescribed 3-pentanol glycosides, i.e., 3-pentyl α-l-arabinofuranosyl-(1′′→6′)-β-d-glucopyranoside, 3-pentyl α-l-rhamnopyranosyl-(1′′→6′)-β-d-glucopyranoside, and 3-pentyl 6′-(3-hydroxy3-methylglutaryl)-β-d-glucopyranoside, as well as seventeen known compounds from A. cremastogyne root nodules. 3-Pentanol glycosides are abundantly distributed in root nodules, while they are distributed in stems, roots, leaves and fruits at low/zero levels. A. cremastogyne plants treated by root nodule suspension emit 3-pentanol. This study enriches the knowledge about specialized metabolites in the actinorhizal host, and provides preliminarily information on the signal exchange in the actinorhizal symbiosis between A. cremastogyne and Frankia.
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| 45. |
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| 46. |
- Xue, Lei, 1992, et al.
(författare)
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Optics-simplified DSP for 50 Gb/s PON downstream transmission using 10 Gb/s optical devices
- 2020
-
Ingår i: Journal of Lightwave Technology. - 0733-8724 .- 1558-2213. ; 38:3, s. 583-589
-
Tidskriftsartikel (refereegranskat)abstract
- Directly-modulated laser (DML) is widely employed in intensity modulation and direct detection (IMDD) system due to its low cost and high output power. However, the corresponding frequency chirp is regarded as one of the main disadvantages for its application in passive optical networks (PONs). In this paper, we theoretically analyze the frequency response evolution of DML based system under different chirp and dispersion conditions, proving that the system bandwidth can be improved by interactions between negative dispersion and DML chirp. Based on this concept, we experimentally demonstrated downstream 50 Gb/s PAM4 signal transmission over 20 km single-mode fiber (SMF) access based on the 10 Gb/s DML operating at 1310 nm and avalanche photodiode (APD). A dispersion-shifted fiber (DSF) providing -150 ps/nm dispersion at 1310 nm in the optical line terminal (OLT) is used to pre-equalize the frequency response of bandwidth-limited directly modulated signals in the optical domain. Thanks to our proposed dispersion-supported equalization (DSE) technique, the system bandwidth can be improved by 5 GHz. Feed-forward equalization (FFE), decision feedback equalization (DFE) and Volterra filter are employed to evaluate the signal performance improvement, respectively. By evaluating the receiver sensitivity, the DSE combined with FFE scheme shows 2 dB improvement than the complex Volterra algorithm, indicating its potential to reduce the complexity of digital signal processing (DSP) and therefore a lower cost and power consumption in PON.
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| 47. |
- Xue, Lei, 1992, et al.
(författare)
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SOA pattern effect mitigation by neural network based pre-equalizer for 50G PON
- 2021
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Ingår i: Optics Express. - 1094-4087 .- 1094-4087. ; 29:16, s. 24714-24722
-
Tidskriftsartikel (refereegranskat)abstract
- Semiconductor optical amplifier (SOA) is widely used for power amplification in O-band, particularly for passive optical networks (PONs) which can greatly benefit its advantages of simple structure, low power consumption and integrability with photonics circuits. However, the annoying nonlinear pattern effect degrades system performance when the SOA is needed as a pre-amplifier in PONs. Conventional solutions for pattern effect mitigation are either based on optical filtering or gain clamping. They are not simple or sufficiently flexible for practical deployment. Neural network (NN) has been demonstrated for impairment compensation in optical communications thanks to its powerful nonlinear fitting ability. In this paper, for the first time, NN-based equalizer is proposed to mitigate the SOA pattern effect for 50G PON with intensity modulation and direct detection. The experimental results confirm that the NN-based equalizer can effectively mitigate the SOA nonlinear pattern effect and significantly improve the dynamic range of receiver, achieving 29-dB power budget with the FEC limit at 1e-2. Moreover, the well-trained NN model in the receiver side can be directly placed at the transmitter in the optical line terminal to pre-equalize the signal for transmission so as to simplify digital signal processing in the optical network unit.
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| 48. |
- Yuan, Shuai, et al.
(författare)
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Interleukins and rheumatoid arthritis : bi-directional Mendelian randomization investigation
- 2022
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Ingår i: Seminars in Arthritis & Rheumatism. - : Elsevier. - 0049-0172 .- 1532-866X. ; 53
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: To assess the causality of the associations between interleukins (ILs) and rheumatoid arthritis (RA) using Mendelian randomization (MR) design. Methods: Genetic instruments and summary-level data for ten ILs were obtained from three genome-wide association meta-analyses. Corresponding data on RA were obtained from a meta-analysis of 22 genome-wide association studies (14,361 cases and 43,923 controls) and the FinnGen consortium (6236 cases, 4596 seropositive cases, 1937 seronegative cases, and 172,834 controls). Forward and reverse MR analyses were performed. Results: The odds ratios (ORs) of RA were 2.08 (95% confidence interval (CI), 1.56-2.77; p<0.001), 2.14 (95% CI, 1.85-2.49; p<0.001), and 0.95 (95% CI, 0.92-0.97; p<0.001) for one standard deviation increase in genetically predicted IL-1 beta, IL-6 and IL-6 receptor antagonist (IL-6ra) levels, respectively. There were suggestive associations of genetically predicted IL-1 receptor antagonist (IL-1ra) (OR, 0.85, 95% CI, 0.76, 0.96; p=0.010) and IL-18 (OR, 1.07, 95% CI, 1.00, 1.15; p=0.043) levels with RA risk. Subtype-specific associations were observed for seropositive RA (IL-1 beta, IL-1ra, and IL-6) and seronegative RA (IL-2 receptor alpha subunit, IL-8, and IL-18). Reverse MR analysis found a suggestive association between genetic liability to RA and IL-6 receptor antagonist (change 0.015; 95% CI, 0.003-0.028; p=0.015). Conclusions: This MR study suggests that long-term IL-1 and IL-6 inhibition may reduce the risk of RA, particularly seropositive RA. Upregulations of ILs involved in IL-6 signaling pathways appears to be downstream effects of RA, which supports the blocking IL-6 treatment for RA.
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| 49. |
- Zhang, Xiaotian, et al.
(författare)
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Delafossite NaYTe2 as a transparent conductive material with bipolar conductivity: A first-principles prediction
- 2024
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Ingår i: Journal of Physics and Chemistry of Solids. - : Elsevier BV. - 0022-3697 .- 1879-2553. ; 190
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Tidskriftsartikel (refereegranskat)abstract
- Doping asymmetry is a long-standing issue for the progress of wide-bandgap semiconductors, including also several transparent conductors. However, a few compounds exhibit bipolar conductivity, implying a desired band gap in combination with proper energy positions of the band edges according to the empirical doping limit rule. The present first-principles study of delafossite NaYTe2 reveals that the compound is thermodynamic stable with an optical gap energy of ∼3.0 eV and an ionization potential of 6.2 eV, and the electronic structure is thus in the range for realizing bipolar character as a transparent conductive material. In addition, the analysis of the defect properties strengthens this prediction, especially for high free carrier concentration in NaYTe2, obtained by either extrinsic doping or intrinsic defects under suitable growth conditions.
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| 50. |
- Zheng, Xiaopeng, et al.
(författare)
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Managing grains and interfaces via ligand anchoring enables 22.3%-efficiency inverted perovskite solar cells
- 2020
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Ingår i: NATURE ENERGY. - : Nature Publishing Group. - 2058-7546. ; 5, s. 131-140
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Tidskriftsartikel (refereegranskat)abstract
- Inverted perovskite solar cells have attracted increasing attention because they have achieved long operating lifetimes. However, they have exhibited significantly inferior power conversion efficiencies compared to regular perovskite solar cells. Here we reduce this efficiency gap using a trace amount of surface-anchoring alkylamine ligands (AALs) with different chain lengths as grain and interface modifiers. We show that long-chain AALs added to the precursor solution suppress nonradiative carrier recombination and improve the optoelectronic properties of mixed-cation mixed-halide perovskite films. The resulting AAL surface-modified films exhibit a prominent (100) orientation and lower trap-state density as well as enhanced carrier mobilities and diffusion lengths. These translate into a certified stabilized power conversion efficiency of 22.3% (23.0% power conversion efficiency for lab-measured champion devices). The devices operate for over 1,000 h at the maximum power point under simulated AM1.5 illumination, without loss of efficiency. While perovskite solar cells with an inverted architecture hold great promise for operation stability, their power conversion efficiency lags behind that of conventional cells. Here, Zheng et al. achieve a certified 22.34% efficiency, exploiting alkylamine ligands as grain and interface modifiers.
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