| 1. |
- Blomqvist (Jonsson), Anna, 1967-
(författare)
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Food and Fashion : Water Management and Collective Action among Irrigation Farmers and Textile Industrialists in South India
- 1996
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- In recent years, much ofthe political debate in the West, East aud South has focused on the decentralization of responsibilities from the state to private enterprises and NGOs. But what potential is there for local communities to create their own govenlance structures able to deal with issues up till recently seen as the responsibility of the state? In this thesis, answer to this question is sought by analyzing two case studies from the semi-arid Coimbatore-region in South India from an institutionai perspective. One case concerns the efforts to involve farmers in irrigation water management in the Lower Bhavani Project, while the other focuses on the pressure on textile industrialists in Tirupur city to collectively treat their polluted effluent water.In both cases, the new distribution ofresponsibilities required that groups ofwater users would succeed in establishing new entities for collective action among themselves strong enough to prevent free-riding on a massive scale. Overcoming three main obstacles proved crucial in this process; meeting coordination costs, re-defining the notion of free-riding among resource users, and meeting motivation costs. Factors both within and outsicte the loeal community affected the degree ofsuccess.The distribution and lise of economic, moral and physical power between various actors and the interconnectedness between local and external institutions proved crucial for the establishrnent oflocal govemance stmctures. Moreover, the historical relation between the respective user group and the state has to a large extent affected the goals and strategies oflocal entities of eolleetive action.Clearly, resource management problems at localleve1 can not be solved by simply decentralizing responsibilities from the state to groups ofresource users. Rather, the state could playan important role by initiating, supporting and directing slich local entities of collective action.
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| 2. |
- Carlsson, Jörgen, et al.
(författare)
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Conjugate chemistry and cellular processing of EGF-dextran
- 1999
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Ingår i: Acta Oncologica. - 0284-186X .- 1651-226X. ; 38:3, s. 313-321
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Tidskriftsartikel (refereegranskat)abstract
- Conjugates with specific binding to the epidermal growth factor receptor, EGFR, of interest for radionuclide based imaging and therapy were prepared using mouse epidermal growth factor, mEGF, and dextran. In one type of conjugate, mEGF was coupled to dextran by reductive amination in which the free amino group on the mEGF N-terminal reacted with the aldehyde group on the reductive end of dextran. The end-end coupled conjugate could be further activated by the cyanopyridinium agent CDAP, thereby introducing tyrosines to the dextran part. In the other type of conjugate, the cyanylating procedure using CDAP was applied, first to activate dextran and then allowing for the amino terminus of mEGF to randomly attach to the dextran. In the latter case, radionuclide-labelled tyrosines or glycines could be added in the same conjugation step. All types of mEGF-dextran conjugates had EGFR-specific binding since the binding could be displaced by an excess of non-radioactive mEGF. The conjugates were to a large extent internalized in the test cells and the associated radioactivity was retained intracellularly for different times depending on both the type of cells and conjugate applied. Different intracellular 'traffic routes' for the radionuclides are discussed as well as applications for both imaging and therapy.
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| 3. |
- Dahlgren, Claes, 1949, et al.
(författare)
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The lysosomal membrane glycoproteins Lamp-1 and Lamp-2 are present in mobilizable organelles, but are absent from the azurophil granules of human neutrophils.
- 1995
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Ingår i: The Biochemical journal. - 0264-6021. ; 311 ( Pt 2), s. 667-74
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Tidskriftsartikel (refereegranskat)abstract
- The subcellular localization of two members of a highly glycosylated protein group present in lysosomal membranes in most cells, the lysosome-associated membrane proteins 1 and 2 (Lamp-1 and Lamp-2), was examined in human neutrophil granulocytes. Antibodies that were raised against purified Lamp-1 adn Lamp-2 gave a distinct granular staining of the cytoplasm upon immunostaining of neutrophils. Subcellular fractionation was used to separate the azurophil and specific granules from a light-membrane fraction containing plasma membranes and secretory vesicles, and Western blotting was used to determine the presence of the Lamps in these fractions. The results show that Lamp-1 and Lamp-2 are present in the specific-granule-enriched fraction and in the light-membrane fraction, but not in the azurophil granules. Separation of secretory vesicles from plasma membranes disclosed that the light-membrane Lamps were present primarily in the secretory-vesicle-enriched fraction. During phagocytosis both Lamp-1 and Lamp-2 became markedly concentrated around the ingested particle and they both appear on the cell surface when the secretory organelles are mobilized.
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| 4. |
- Johansson, A, et al.
(författare)
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Different subcellular localization of cytochrome b and the dormant NADPH-oxidase in neutrophils and macrophages: effect on the production of reactive oxygen species during phagocytosis.
- 1995
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Ingår i: Cellular immunology. - : Elsevier BV. - 0008-8749. ; 161:1, s. 61-71
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Tidskriftsartikel (refereegranskat)abstract
- When neutrophils and macrophages phagocytose a prey, e.g., complement (C3b)-opsonized yeast particles, the oxygen radical generating NADPH-oxidase is activated. In neutrophils, most of the production of oxygen metabolites occurred in an intracellular compartment, possibly in the phagolysosome. In contrast, no intracellular production could be detected in human macrophages. In these cells, the subcellular localization of the superoxide-generating NADPH-oxidase and associated cytochrome b was assessed in intact cells with indirect immunofluorescence and confocal laser scanning microscopy, and with subcellular fractionation, using centrifugation on Percoll density gradients. A dual localization of the cytochrome b as well as the dormant NADPH-oxidase activity in neutrophils was in agreement with earlier immunocytochemical, biochemical, and subcellular fractionation studies. Furthermore, most of the activity was recovered from the specific granules, whereas only a small fraction was retained in the plasma membrane. In contrast, the cytochrome b/NADPH-oxidase activity in macrophages localized primarily in the plasma membrane fraction. We suggest that the macrophages are incapable of producing reactive oxygen species intraphagosomally, due to an absence of a granule-localized pool of the membrane components of the NADPH-oxidase.
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| 5. |
- Khalfan, L, et al.
(författare)
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Lack of correlation between NADPH-oxidase priming and elevated alkaline phosphatase activity in cord blood neutrophils.
- 1995
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Ingår i: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology. - 0905-6157. ; 6:3, s. 161-4
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Tidskriftsartikel (refereegranskat)abstract
- Human neutrophils were isolated from cord blood drawn after Caesarean section deliveries without labour and from peripheral blood from healthy adults. The alkaline phosphatase (ALP) activity in the cell populations was compared with the release of respiratory burst products after activation with the chemoattractant n-formyl-methionyl-leucyl-phenylalanine (fMLP). In contrast to cord blood neutrophils obtained from babies after normal vaginal deliveries, the Caesarean section neutrophils were not metabolically primed. However, like the neutrophils from vaginally delivered babies, the Caesarean section neutrophils showed an increased ALP activity compared to adult control neutrophils. These findings indicate that priming of cord blood neutrophils appears to be induced by labour and can be dissociated from increased ALP activity.
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| 6. |
- Lubberink, Mark, et al.
(författare)
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Positron emission tomography and radioimmunotargeting : aspects ofquantification and dosimetry
- 1999
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Ingår i: Acta Oncologica. - 0284-186X .- 1651-226X. ; 38:3, s. 343-349
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Tidskriftsartikel (refereegranskat)abstract
- Positron emission tomography (PET) is a medical imaging tool with high resolution and good quantitative properties, which makes it suitable for in vivo quantification of radioimmunotargeting agents. Most radionuclides used in radioimmunotherapy have positron-emitting analogues, which can be used for PET imaging, and this opens the possibility of performing dosimetry with PET. These isotopes, however, often emit gamma radiation and high-energy positrons in their decay, influencing the imaging properties of PET. Spatial resolution, reconstructed background and line source recovery for a number of non-pure positron emitters were investigated and compared with the imaging properties of 18F. PET imaging properties did not degrade severely for these non-pure positron emitters, but caution has to be applied when doing quantitative measurements. To assess the possibility of conducting PET studies during therapy, by combining, for example, a small amount of 124I with 131I, the influence of the presence of large amounts of gamma radiation on PET count rate characteristics was studied. The results of these studies were related to the necessary amounts of radioactivity needed for treatment of post-operative remains of glioma. The results indicate that the count rate capabilities of 2D PET permit PET studies for dose evaluation during radioimmunotherapy.
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| 7. |
- Lundqvist, Anna, et al.
(författare)
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Neuropsychological Aspects of Driving After Brain Lesion: Simulator Study and On-Road Driving
- 1997
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Ingår i: Applied neuropsychology. - : Informa UK Limited. - 0908-4282 .- 1532-4826. ; 4:4, s. 220-230
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Tidskriftsartikel (refereegranskat)abstract
- 29 patients with brain lesion and 29 matched controls completed a neuropsychological test battery to assess perceptual, cognitive, and executive functioning and were tested on driving performance in a simulator and actual road conditions. The patients were socially well recovered with a high rate of employment. Patients performed significantly worse than controls on the test battery, especially on executive and cognitive functions. Patients drove as well as controls in predictable situations in the advanced simulator used In unpredictable situations, patients demonstrated longer reaction time (RT) and safety margins, as well as difficulties in allocating processing resources to a secondary task. Patients showed significantly less attention, worse traffic behavior, and less risk awareness when driving in real traffic. 41% of the patients did not pass the driving test. The neuropsychological test battery was factor analyzed into 4 factors: executive capacity, cognitive capacity, automatic attentional capacity, and simple perceptual-motor capacity. The second factor was the most significant, with a simultaneous capacity test predicting driving performance with 78% confidence.
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| 8. |
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| 9. |
- Lundqvist, Björn, et al.
(författare)
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Cutoff of the vortex-glass behavior in Tl(2)Ba(2)CaCu(2)O(8+delta) superconducting thin films
- 1998
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Ingår i: Physical Review B. Condensed Matter and Materials Physics. - 1098-0121 .- 1550-235X. ; 58:10, s. 6580-6584
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Tidskriftsartikel (refereegranskat)abstract
- The vortex dynamics of a Tl(2)Ba(2)CaCu(2)O(8+delta) thin film has been measured by electrical resistivity and I-V characteristics. The temperature T*(B) defined from a vortex-glass analysis of the resistivity in the vortex liquid state using the Vogel-Fulcher relation is shown to be consistent with a vortex-glass-like scaling of the I-V characteristics in the high dissipation regime. Below T*, there is however a significant temperature range in which the flux creep does not vanish at low currents. This suggests that the transition into a glassy vortex state is cut off at some length scale.
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| 10. |
- Lundqvist, Hans, et al.
(författare)
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Positron emission tomography and radioimmunotargeting : general aspects
- 1999
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Ingår i: Acta Oncologica. - 0284-186X .- 1651-226X. ; 38:3, s. 335-341
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Tidskriftsartikel (refereegranskat)abstract
- To optimize radioimmunotherapy, in vivo information on individual patients, such as radionuclide uptake, kinetics, metabolic patterns and optimal administration methods, is important. An overriding problem is to determine accurately the absorbed dose in the target organ as well as critical organs. Positron Emission Tomography (PET) is a superior technique to quantify regional kinetics in vivo with a spatial resolution better than 1 cm3 and a temporal resolution better than 10 s. However, target molecules often have distribution times of several hours to days. Conventional PET nuclides are not applicable and alternative positron-emitting nuclides with matching half-lives and with suitable labelling properties are thus necessary. Over many years we have systematically developed convenient production methods and labelling techniques of suitable positron nuclides, such as 110In(T(1/2) = 1.15 h), 86Y(T(1/2) = 14 h), 76Br(T(1/2) = 16 h) and 124I(T(1/2) = 4 days). 'Dose planning' can be done, for example, with 86Y- or 124I-labelled ligands before therapy, and 90Y- and 131I-labelled analogues and double-labelling, e.g. with a 86Y/90Y-labelled ligand, can be used to determine the true radioactivity integral from a pure beta-emitting nuclide. The usefulness of these techniques was demonstrated in animal and patient studies by halogen-labelled MAbs and EGF-dextran conjugates and peptides chelated with metal ions.
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| 11. |
- Lövqvist, Anna, et al.
(författare)
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76Br-labeled monoclonal anti-CEA antibodies for radioimmuno positron emission tomography
- 1995
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Ingår i: Nuclear Medicine and Biology. - 0969-8051 .- 1872-9614. ; 22:1, s. 125-131
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Tidskriftsartikel (refereegranskat)abstract
- For the application of anti-tumor monoclonal antibodies (MAbs) in positron emission tomography (PET), labeling radionuclides with half-lives allowing a suitable time frame for imaging are required. The anti-CEA MAb 38S1 was labeled with the positron emitting nuclide 76Br (t1/2 16 h) using bromoperoxidase (BPO), and subsequently affinity purified. A procedure was devised to allow reproducible production of MAb-preparations of high immunoreactivity and with acceptable bromination yield. The biological activity of 76Br-38S1 was retained and comparable to that of chloramine-T labeled 125I-38S1, as tested in vitro.
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| 12. |
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| 13. |
- Lövqvist, Anna, et al.
(författare)
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Comparative PET imaging of experimental tumors with bromine-76-labeled antibodies, fluorine-18-fluorodeoxyglucose and carbon-11-methionine
- 1997
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Ingår i: Journal of Nuclear Medicine. - 0161-5505 .- 1535-5667. ; 38:7, s. 1029-1035
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Tidskriftsartikel (refereegranskat)abstract
- The potential of a 76Br-labeled anti-carcinoembryonic antigen monoclonal antibody (MAb), 38S1, as tumor-imaging agent for PET was investigated in a comparative experimental study with [18F]fluorodeoxyglucose ([18F]FDG) and L-[methyl-11C]methionine ([11C]Met). METHODS: The three radiotracers were administered to nude rats carrying subcutaneous xenografts or liver metastases from a human colonic carcinoma. Tracer biodistribution was evaluated by PET imaging and radioactivity measurement of dissected tissues and also by whole-body autoradiography for subcutaneous xenografts. RESULTS: For PET imaging of subcutaneous tumors, 76Br-38S1 proved superior to the other radiotracers. Tumor-to-tissue ratios were, except for the tumor-to-blood ratio, generally higher for 76Br-labeled MAb than for [18F]FDG and [11C]Met. Liver metastases were imaged with PET using both 76Br-38S1 and [18F]FDG, and the metastases-to-liver ratios of dissected samples were not significantly different for the two radiotracers. CONCLUSION: The tumor-imaging capacity of 76Br-labeled MAb 38S1 was superior to [18F]FDG and [11C]Met in the subcutaneous tumor model, whereas 76Br-38S1 and [18F]FDG were equally successful for the identification of liver metastases.
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| 14. |
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| 15. |
- Lövqvist, Anna, et al.
(författare)
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Kinetics of 76Br-labeled anti-CEA antibodies in pigs : aspects of dosimetry and PET imaging properties
- 1999
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Ingår i: Medical physics (Lancaster). - : Wiley. - 0094-2405. ; 26:2, s. 249-258
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Tidskriftsartikel (refereegranskat)abstract
- A monoclonal antibody labeled with the positron-emitting radionuclide 76Br (T(1/2) 16.2 h) has previously been shown useful for positron emission tomography (PET) imaging of experimental tumors. Our aim in the present study was to investigate the effects of the complex decay scheme of this radionuclide on normal organ dosimetry and PET image quality. Three mini-pigs were injected intravenously with 46-75 MBq of the 76Br-labeled anti-CEA antibody 38S1, and the whole-body kinetics followed by PET imaging for 19 h. From PET data, absorbed doses in human organs were estimated using the MIRDOSE 3.0 software. The highest 76Br concentrations were found in lungs, after a correction for the air volume in this organ. The lungs received the highest absorbed dose (mGy/MBq, mean+/-maximum error), 0.84+/-0.16, followed by liver, 0.74+/-0.28, and small intestine, 0.55+/-0.05, while the effective dose equivalent was 0.41+/-0.03 mSv/MBq. The PET imaging properties of 76Br in a two-dimensional 2D PET camera, including central area resolution and scattering effects, were investigated in phantoms and compared to those of 18F. In a 0.97 g/cm3 material, approximating soft tissue density, the FMHW ("full width at half-maximum") value of the point spread function was 7.7+/-0.2 mm for 76Br and 6.0+/-0.1 mm for 18F. In conclusion, radioimmuno PET using 76Br-labeled antibodies resulted in a fairly even distribution of the radiation dose, where the highest absorbed organ doses were only about two to three times higher than the mean absorbed body dose. The high energy beta+ spectrum in the 76Br decay had only minor effects on the resolution, but may decrease the quantification accuracy, especially in organs with a lower density such as a lung.
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| 16. |
- Lövqvist, Anna, et al.
(författare)
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Pharmacokinetics and experimental PET imaging of a bromine-76-labeled monoclonal anti-CEA antibody
- 1997
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Ingår i: Journal of Nuclear Medicine. - 0161-5505 .- 1535-5667. ; 38:3, s. 395-401
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Tidskriftsartikel (refereegranskat)abstract
- Bromine-76 is potentially useful as a radiolabel for monoclonal antibodies (MAbs) in PET imaging. The purpose of the present study was to evaluate the 76Br-labeled anticarcinoembryonic antigen (-CEA) MAb 38S1 as a tumor imaging agent in an experimental tumor model and to study the pharmacokinetics of 76Br-38S1 in comparison with 125I-38S1. METHODS: Nude rats carrying human colon carcinoma xenografts were co-injected with directly labeled 76Br-38S1 and 125I-38S1. Biodistribution of labeled 38S1 was monitored for 4 days after administration, in the case of 76Br activity, including PET imaging. In addition, catabolism of radiolabeled MAbs was analyzed by gel filtration chromatography of blood plasma and homogenized tissues. RESULTS: Tumor sites could be readily identified by PET imaging from 46 hr after administration of 76Br-38S1 and onwards. The concentration of 76Br activity in tumors, blood and most normal tissues was higher than the corresponding 125I concentration at all time points. This was mainly due to catabolism of radiolabeled MAb, resulting in free radiohalides, of which 76Br- was retained in contrast to the rapidly excreted 125I- ion. CONCLUSION: Bromine-76-labeled anti-CEA MAbs may be applied for experimental tumor imaging with PET.
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| 17. |
- Tolmachev, Vladimir, et al.
(författare)
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Closo-dodecaborate(2-) as a linker for iodination of macromolecules : Aspects on conjugation chemistry and biodistribution
- 1999
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Ingår i: Bioconjugate chemistry. - : American Chemical Society (ACS). - 1043-1802 .- 1520-4812. ; 10:3, s. 338-45
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Tidskriftsartikel (refereegranskat)abstract
- Boron-containing compounds like closo-dodecaborate(2-) are in theory suitable for radioactive labeling with halogens. The boron-halogen bond is stronger than carbon-halogen bond and is not likely to be recognized by deiodinating enzymes in vivo. Peptides and proteins may be conjugated with various closo-dodecaborate(2-)-containing ligands, and thereafter, the conjugate can be iodinated. Since closo-dodecaborate(2-) is more avidly iodinated than tyrosine in moderately acidic media, such conjugates may be directly labeled on the boron part with radioisotopes of iodine using the standard Chloramine-T procedure. Mercapto-undecahydro-closo-dodecaborate(2-) (BSH) was reacted with the double bond of allyldextran to form a boronated dextran compound of the molecular size of about 70 kDa. This compound, in the text denoted as Dx-BS, and cesium dodecahydro-closo-dodecaborate(2-) were labeled using iodine-125. The two compounds were administered to rats in order to study their in vivo stability. The results indicate that iodinated Dx-BS is stable for about 20 h in vivo. The degradation rate, as indicated by thyroid uptake, was found low. [125I]Iodo-closo-dodecaborate(2-), which is a possible degradation product of [125I]Dx-BS-I, was rapidly excreted in urine without significant accumulation in any organ.
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