| 1. |
- Björklund, Anna, et al.
(författare)
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Energiplanering med strategisk miljöbedömning i Finspång
- 2007
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Denna rapport är en del av slutrapporteringen från två av projekten i programmet ”Miljöstrategiska verktyg”. Projekten är Utveckling av verktyg för strategisk miljöbedömning och medborgarmedverkan (MiSt-projekt 1) samt Scenariometoder i strategisk miljöbedömning (MiSt-projekt 7). I samarbete med Finspångs kommun har ett gemensamt projekt utvecklats för att ta fram en ny energiplan i kommunen. Programmet "Miljöstrategiska verktyg", MiSt, är ett tvärvetenskapligt forskningsprogram finansierat av Naturvårdsverket. Programmet leds från Blekinge Tekniska Högskola. I programmet studeras verktyg som kan underlätta miljöbedömning i strategiskt beslutsfattande på olika nivåer, från nationell till lokal. Förståelsen och basen för utveckling av verktyg och rekommendationer om verktyg i olika planerings- och beslutsprocesser baseras på empirisk forskning på fall inom flera sektorer. Målen för MiSt-programmet: • Kritisk undersökning av verktygens funktion • Teoribaserad förståelse av deras verkan • Utveckling av råd om effektiv användning av verktyg och kombinationer avverktyg
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| 2. |
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| 3. |
- Börjesson, Karl, 1982, et al.
(författare)
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A membrane anchored DNA-based energy/electron transfer assembly
- 2008
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Ingår i: Nucleic acids symposium series (2004). - 1746-8272. ; :52, s. 691-691
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Tidskriftsartikel (refereegranskat)abstract
- In this work the trapping and conversion of visible light energy into chemical energy is examined using a supramolecular assembly. This consists of a light absorbing antenna and a porphyrin redox centre both covalently attached to a DNA strand, which in turn is bound to a lipid membrane. The excitation energy is finally trapped as a benzoquinone radical anion that could potentially be used in subsequent chemical reactions.
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| 4. |
- Börjesson, Karl, 1982, et al.
(författare)
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Characterization and use of tricyclic fluorescent nucleic acid base analogues
- 2008
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Ingår i: Nucleic acids symposium series (2004). - 1746-8272. ; :52, s. 3-4
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Tidskriftsartikel (refereegranskat)abstract
- The two recently developed nucleic acid probe molecules tC and tC(O) both have unique properties compared to other molecules in the family of fluorescent base analogues.(1-5) These tricyclic base analogues both form very stable base pairs with guanine and give minimal perturbations to the native structure of DNA.(2) We have found that tC(O) is the brightest fluorescent base analogue reported(4) and that tC also is very bright and has a fluorescence quantum yield that is virtually insensitive to its surrounding microenvironment within the nucleic acid(3). These base analogues have so far been used in FRET-studies of a DNA-polymerase system(6) and in initial anisotropy-studies of DNA-containing systems(4).
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| 5. |
- Börjesson, Karl, 1982, et al.
(författare)
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Membrane-Anchored DNA Assembly for Energy and Electron Transfer
- 2009
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 1520-5126 .- 0002-7863. ; 131:8, s. 2831-2839
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Tidskriftsartikel (refereegranskat)abstract
- In this work we examine the trapping and conversion of visible light energy into chemical energy using a supramolecular assembly. The assembly consists of a light-absorbing antenna and a porphyrin redox center, which are covalently attached to two complementary 14-mer DNA strands, hybridized to form a double helix and anchored to a lipid membrane. The excitation energy Is finally trapped In the lipid phase of the membrane as a benzoquinone radical anion that could potentially be used In subsequent chemical reactions. In addition, In this model complex, the hydrophobic porphyrin moiety acts as an anchor into the liposome positioning the DNA construct on the lipid membrane surface. The results show the suitability of our system as a prototype for DNA-based light-harvesting devices, In which energy transfer from the aqueous phase to the interior of the lipid membrane Is followed by charge separation. © 2009 American Chemical Society.
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| 6. |
- Kreij, Karl, 1975-, et al.
(författare)
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On-line detection of microbial contaminations in animal cell reactor cultures using an electronic nose device
- 2005
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Ingår i: Cytotechnology (Dordrecht). - : Springer Science and Business Media LLC. - 0920-9069 .- 1573-0778. ; 48:1-3, s. 41-58
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Tidskriftsartikel (refereegranskat)abstract
- An electronic nose (EN) device was used to detect microbial and viral contaminations in a variety of animal cell culture systems. The emission of volatile components from the cultures accumulated in the bioreactor headspace, was sampled and subsequently analysed by the EN device. The EN, which was equipped with an array of 17 chemical gas sensors of varying selectivity towards the sampled volatile molecules, generated response patterns of up to 85 computed signals. Each 15 or 20 min a new gas sample was taken generating a new response pattern. A software evaluation tool visualised the data mainly by using principal component analysis. The EN was first used to detect microbial contaminations in a Chinese hamster ovary (CHO) cell line producing a recombinant human macrophage colony stimulating factor (rhM-CSF). The CHO cell culture was contaminated by Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida utilis which all were detected. The response patterns from the CHO cell culture were compared with monoculture references of the microorganisms. Second, contaminations were studied in an Sf-9 insect cell culture producing another recombinant protein (VP2 protein). Contaminants were detected from E. coli, a filamentous fungus and a baculovirus. Third, contamination of a human cell line, HEK-293, infected with E. coli exhibited comparable results. Fourth, bacterial contaminations could also be detected in cultures of a MLV vector producer cell line. Based on the overall experiences in this study it is concluded that the EN method has in a number of cases the potential to be developed into a useful on-line contamination alarm in order to support safety and economical operation for industrial cultivation. © Springer 2005.
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| 7. |
- Mårtensson, Karl, et al.
(författare)
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Dynamic Model Predictive Control
- 2008
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Konferensbidrag (refereegranskat)abstract
- In this paper an alternative approach to model predictive control is presented. In traditional MPC a finite horizon open loop optimal control problem is solved in each sampling instance. When uncertainties such as computational delays are present, one can encounter problems. We propose to parametrize the control sequence in each sampling instant in terms of a linear feedback controller, i.e. in each sample a dynamic feedback compensator is computed. Thus, if computational delays are present the control system runs in closed loop, decreasing the need for ad hoc solutions used in traditional MPC.
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| 8. |
- Mårtensson, Karl, et al.
(författare)
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Gradient methods for iterative distributed control synthesis
- 2009
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Konferensbidrag (refereegranskat)abstract
- In this paper we present a gradient method to iteratively update local controllers of a distributed linear system driven by stochastic disturbances. The control objective is to minimize the sum of the variances of states and inputs in all nodes. We show that the gradients of this objective can be estimated distributively using data from a forward simulation of the system model and a backward simulation of the adjoint equations. Iterative updates of local controllers using the gradient estimates gives convergence towards a locally optimal distributed controller.
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| 9. |
- Mårtensson, Ulrika, et al.
(författare)
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Deletion of the G protein-coupled receptor 30 impairs glucose tolerance, reduces bone growth, increases blood pressure, and eliminates estradiol-stimulated insulin release in female mice.
- 2009
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Ingår i: Endocrinology. - : The Endocrine Society. - 1945-7170 .- 0013-7227. ; 150:2, s. 687-98
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Tidskriftsartikel (refereegranskat)abstract
- In vitro studies suggest that the G protein-coupled receptor (GPR) 30 is a functional estrogen receptor. However, the physiological role of GPR30 in vivo is unknown, and it remains to be determined whether GPR30 is an estrogen receptor also in vivo. To this end, we studied the effects of disrupting the GPR30 gene in female and male mice. Female GPR30((-/-)) mice had hyperglycemia and impaired glucose tolerance, reduced body growth, increased blood pressure, and reduced serum IGF-I levels. The reduced growth correlated with a proportional decrease in skeletal development. The elevated blood pressure was associated with an increased vascular resistance manifested as an increased media to lumen ratio of the resistance arteries. The hyperglycemia and impaired glucose tolerance in vivo were associated with decreased insulin expression and release in vivo and in vitro in isolated pancreatic islets. GPR30 is expressed in islets, and GPR30 deletion abolished estradiol-stimulated insulin release both in vivo in ovariectomized adult mice and in vitro in isolated islets. Our findings show that GPR30 is important for several metabolic functions in female mice, including estradiol-stimulated insulin release.
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| 10. |
- Sandin, Peter, 1977, et al.
(författare)
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Characterization and Use of an Unprecedentedly Bright and Structurally Non-Perturbing Fluorescent DNA Base Analogue
- 2008
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Ingår i: Nucleic Acids Research. - : Oxford University Press (OUP). - 0305-1048 .- 1362-4962. ; 36:1, s. 157-167
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Tidskriftsartikel (refereegranskat)abstract
- This article presents the first evidence that the DNA base analogue 1,3-diaza-2-oxophenoxazine, tC(O), is highly fluorescent, both as free nucleoside and incorporated in an arbitrary DNA structure. tC(O) is thoroughly characterized with respect to its photophysical properties and structural performance in single- and double-stranded oligonucleotides. The lowest energy absorption band at 360 nm (epsilon = 9000 M-1 cm(1)) is dominated by a single in-plane polarized electronic transition and the fluorescence, centred at 465 nm, has a quantum yield of 0.3. When incorporated into double-stranded DNA, tC(O) shows only minor variations in fluorescence intensity and lifetime with neighbouring bases, and the average quantum yield is 0.22. These features make tC(O), on average, the brightest DNA-incorporated base analogue so far reported. Furthermore, it base pairs exclusively with guanine and causes minimal perturbations to the native structure of DNA. These properties make tC(O) a promising base analogue that is perfectly suited for e.g. photophysical studies of DNA interacting with macromolecules (proteins) or for determining size and shape of DNA tertiary structures using techniques such as fluorescence anisotropy and fluorescence resonance energy transfer (FRET).
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