1. |
- Coulibaly, S, et al.
(författare)
-
Modulation of invasive properties of murine squamous carcinoma cells by heterologous expression of cathepsin B and cystatin C
- 1999
-
Ingår i: International Journal of Cancer. - 0020-7136. ; 83:4, s. 526-531
-
Tidskriftsartikel (refereegranskat)abstract
- Murine SCC-VII squamous carcinoma cells have the capacity to penetrate reconstituted basement membranes (Matrigel) in vitro. The invasion of Matrigel layers by SCC-VII cells was significantly reduced by E-64, a specific inhibitor of lysosomal cysteine proteinases. The cathepsin-B-selective E-64 derivative, CA-074, inhibited penetration of Matrigel by SCC-VII cells to the same extent, indicating a major role for this particular lysosomal enzyme in extracellular-matrix degradation during squamous-carcinoma-cell invasion. SCC-VII cells were stably transfected with a cDNA encoding human procathepsin B, in an attempt to modulate the invasive properties of the cell line. The transfected cells expressed the heterologous gene, secreted increased amounts of procathepsin B and displayed enhanced invasive potential. In vivo, the activity of cathepsin B is strictly regulated by endogenous inhibitors. SCC-VII cells were therefore also stably transfected with a cDNA encoding human cystatin C, the most potent cysteine-proteinase inhibitor in mammalian tissues. The expression of this transgene resulted in the production of active recombinant cystatin C and a pronounced reduction in Matrigel invasion. These studies demonstrate that the invasive properties of squamous-cell carcinomas can be changed by modulation of the balance between cathepsin B and its endogenous inhibitors, and provide further evidence for the involvement of this lysosomal cysteine proteinase in tumour invasion and metastasis.
|
|
2. |
- Gulda, K, et al.
(författare)
-
Quadrupole deformed and octupole collective bands in Ra-228
- 1998
-
Ingår i: NUCLEAR PHYSICS A. - : ELSEVIER SCIENCE BV. - 0375-9474. ; 636:1, s. 28-46
-
Tidskriftsartikel (refereegranskat)abstract
- Spins and parities for collective states in Ra-228 have been determined from conversion electron measurements with a mini-orange beta spectrometer. The fast-timing beta gamma gamma(t) method has been used to measure lifetimes of T-1/2 = 550(20) ps and 181
|
|
3. |
- Kurcewicz, W, et al.
(författare)
-
The nuclear structure of Fr-227
- 1997
-
Ingår i: NUCLEAR PHYSICS A. - : ELSEVIER SCIENCE BV. - 0375-9474. ; 621:4, s. 827-852
-
Tidskriftsartikel (refereegranskat)abstract
- The gamma-rays following the beta(-) decay of Rn-227 have been investigated by means of gamma-ray singles and gamma gamma-coincidence measurements using an array of 12 Compton-suppressed Ge detectors. The fast-timing beta gamma gamma(t) method has been us
|
|
4. |
|
|