| 1. |
- Mamma, D., et al.
(författare)
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An alternative approach to the bioconversion of sweet sorghum carbohydrates to ethanol
- 1995
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Ingår i: Biomass and Bioenergy. - 0961-9534 .- 1873-2909. ; 8:2, s. 99-103
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Tidskriftsartikel (refereegranskat)abstract
- The ethanol fermentation of juice and press cake, resulting from the squeezing of sweet sorghum stalks at high pressure, was investigated. The juice was fermented by Saccharomyces cerevisiae and yielded 4.8 g ethanol per 100 g of fresh stalks. The press cake was fermented directly to ethanol by a mixed culture of Fusarium oxysporum and Saccharomyces cerevisiae and yielded 5.1 g ethanol per 100 g of fresh stalks. An overall ethanol concentration and yield of 5.6% (w/v) and 9.9 g of ethanol per 100 g of fresh stalks respectively was obtained. Based on soluble carbohydrates, the ethanol yield from press cake was doubled while the overall theoretical yield was enhanced by 20.7% due to the bioconversion of a significant portion of cell wall polysaccharides to ethanol. The process was found promising for further investigation.
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| 2. |
- Christakopoulos, Paul, et al.
(författare)
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Enhanced acetyl esterase production by Fusarium oxysporum
- 1999
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Ingår i: World Journal of Microbiology & Biotechnology. - 0959-3993 .- 1573-0972. ; 15:4, s. 443-446
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Tidskriftsartikel (refereegranskat)abstract
- Production of acetyl esterase (EC 3.1.1.6) by Fusarium oxysporum strain F3 was enhanced by optimization of growth conditions. Under optimal conditions, activities as high as 0.89 U/ml of culture medium were obtained. The culture filtrate was equally active on p-nitrophenyl acetate and acetylxylan. The enzyme produced 71% deacetylation of acetylxylan in 2 h at 40 ∘C. Activity was optimized at pH6.5 and at 55 ∘C. The respective Km values for p-nitrophenyl acetate and acetylxylan were 0.25 mM and 1.05% (w/v) and the Vm values were 0.65 and 0.43 μmol acetate/min/mg protein.
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| 3. |
- Christakopoulos, Paul, et al.
(författare)
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Production and partial characterization of xylanase from Fusarium oxysporum
- 1996
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Ingår i: Bioresource Technology. - 0960-8524 .- 1873-2976. ; 58:2, s. 115-119
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Tidskriftsartikel (refereegranskat)abstract
- Production of xylanase by Fusarium oxysporum strain F3 was enhanced by optimization of initial pH of the culture medium, the type and concentration of nitrogen and carbon source, and the growth temperature. Under these conditions, yields as high as 245 U/ml of culture medium were obtained. The most important characteristic of the enzyme is its high pH stability. It retained 80 and 66% of the activity at pH 9.0 after 24 h at 4 and 30°C, respectively. Chromogenic (fluorogenic) 4-methylumbelliferyl-β-glycosides of xylose (MUX) and xylobiose (MUX2) were used to characterize xylanase multienzyme components, after separation by isoelectric focusing. The zymogram indicated one major, one minor xylanase and one active β-xylosidase exhibiting pI values of 9.5, 6.5 and 3.8, respectively
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| 4. |
- Christakopoulos, Paul, et al.
(författare)
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Production of an esterase from Fusarium oxysporum catalysing transesterification reactions in organic solvents
- 1998
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Ingår i: Process Biochemistry. - 1359-5113 .- 1873-3298. ; 33:7, s. 729-733
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Tidskriftsartikel (refereegranskat)abstract
- The production of an esterase by Fusarium oxysporum, grown on tomato skins as the sole carbon source, was studied in submerged and solid state cultures. Under optimum growth conditions, enzyme yields as high as 7·3 U/ml of culture medium and 19·4 U/g of carbon source were obtained. The esterase catalysed the synthesis of esters in organic solvents. Geraniol was transacetylated in hexane by the esterase using triacetyl as an acetyl donor. The geranyl acetate yield was 68%.
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