| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Menkveld, Albert J., et al.
(författare)
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Nonstandard Errors
- 2024
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Ingår i: JOURNAL OF FINANCE. - : Wiley-Blackwell. - 0022-1082 .- 1540-6261. ; 79:3, s. 2339-2390
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Tidskriftsartikel (refereegranskat)abstract
- In statistics, samples are drawn from a population in a data-generating process (DGP). Standard errors measure the uncertainty in estimates of population parameters. In science, evidence is generated to test hypotheses in an evidence-generating process (EGP). We claim that EGP variation across researchers adds uncertainty-nonstandard errors (NSEs). We study NSEs by letting 164 teams test the same hypotheses on the same data. NSEs turn out to be sizable, but smaller for more reproducible or higher rated research. Adding peer-review stages reduces NSEs. We further find that this type of uncertainty is underestimated by participants.
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| 3. |
- Townend, Paul, et al.
(författare)
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COGNIT: challenges and vision for a serverless and multi-provider cognitive cloud-edge continuum
- 2023
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Ingår i: 2023 IEEE International Conference on Edge Computing and Communications (EDGE). - : IEEE. - 9798350304831 - 9798350304848 ; , s. 12-22
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Konferensbidrag (refereegranskat)abstract
- Use of the serverless paradigm in cloud application development is growing rapidly, primarily driven by its promise to free developers from the responsibility of provisioning, operating, and scaling the underlying infrastructure. However, modern cloud-edge infrastructures are characterized by large numbers of disparate providers, constrained resource devices, platform heterogeneity, infrastructural dynamicity, and the need to orchestrate geographically distributed nodes and devices over public networks. This presents significant management complexity that must be addressed if serverless technologies are to be used in production systems. This position paper introduces COGNIT, a major new European initiative aiming to integrate AI technology into cloud-edge management systems to create a Cognitive Cloud reference framework and associated tools for serverless computing at the edge. COGNIT aims to: 1) support an innovative new serverless paradigm for edge application management and enhanced digital sovereignty for users and developers; 2) enable on-demand deployment of large-scale, highly distributed and self-adaptive serverless environments using existing cloud resources; 3) optimize data placement according to changes in energy efficiency heuristics and application demands and behavior; 4) enable secure and trusted execution of serverless runtimes. We identify and discuss seven research challenges related to the integration of serverless technologies with multi-provider Edge infrastructures and present our vision for how these challenges can be solved. We introduce a high-level view of our reference architecture for serverless cloud-edge continuum systems, and detail four motivating real-world use cases that will be used for validation, drawing from domains within Smart Cities, Agriculture and Environment, Energy, and Cybersecurity.
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