Comparison of three common molecular tools for distinguishing among geographically separated clones of the diatom Skeletonema marinoi Sarno et Zingone (bacillariophyceae)

• Skeletonema marinoi Sarno et Zingone is a planktonic marine diatom with a widespread geographic distribution. Different populations of this species may show distinct genetic signatures. We have evaluated the utility of three common molecular methods for distinguishing clones of S. marinoi from different geographic regions. Clonal cultures were isolated from the Canadian west coast, south west Portugal, and the east and west coasts of Sweden. All strains originated from resting stages in sediment. More than 90% of the individually isolated chains grew to densities suitable for DNA extraction. Genetic signatures of clones from each sample location were assessed by sequencing variable domains (D1-D3) of the nuclear large subunit (LSU) rRNA gene and internal transcriber spacer (ITS) (ITS-1, 5.8S and ITS-2) regions, and also by analysis of randomly amplified polymorphic DNA patterns. Analysis of molecular variance showed that strains from the four geographic areas were significantly separated by all three methods but that differences among European samples were best resolved by ITS 2 sequences.

Ämnesord

NATURVETENSKAP  -- Biologi -- Ekologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Ecology (hsv//eng)

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

amova

diatom

genetic variation

its

lsu

rapd

resting stages

skeletonema

subunit ribosomal-rna

polymerase-chain-reaction

genetic-variation

ditylum-brightwellii

phylogenetic-relationships

alexandrium dinophyceae

coastal waters

north-america

spring bloom

diversity

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)