| 1. |
- Lindén, Ola, et al.
(författare)
-
Radioimmunotherapy using 131I-labeled anti-CD22 monoclonal antibody (LL2) in patients with previously treated B-cell lymphomas
- 1999
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Ingår i: Clinical Cancer Research. - 1078-0432. ; 5:10 Suppl, s. 3287-3291
-
Tidskriftsartikel (refereegranskat)abstract
- Experience in using rapidly internalizing antibodies, such as the anti-CD22 antibody, for radioimmunotherapy of B-cell lymphomas is still limited. The present study was conducted to assess the efficacy and toxicity of a 131I-labeled anti-CD22 monoclonal antibody (mAb), LL2, in patients with B-cell lymphomas failing first- or second-line chemotherapy. Eligible patients were required to have measurable disease, less than 25% B cells in unseparated bone marrow, and an uptake of 99mTc-labeled LL2Fab' in at least one lymphoma lesion on immunoscintigram. Eight of nine patients examined with immunoscintigraphy were unequivocally found to have an uptake, and therapy with 131I-labeled anti-CD22 [1330 MBq/m2 (36 mCi/m2)] preceded by 20 mg of naked anti-CD22 mAb was administered. Three patients achieved partial remission (duration, 12, 3, and 2 months), and one patient with progressive lymphoma showed stable disease for 17 months. Four patients exhibited progressive disease. The toxicity was hematological. Patients with subnormal counts of neutrophils or platelets before therapy seemed to be more at risk for hematological side effects. Radioimmunotherapy in patients with B-cell lymphomas using 131I-labeled mouse anti-CD22 can induce objective remission in patients with aggressive as well as indolent lymphomas who have failed prior chemotherapy.
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| 2. |
- Andersson, Michael K., et al.
(författare)
-
Bootstrap testing for fractional integration
- 1998
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Asymptotic tests for fractional integration, such as the Geweke-Porter-Hudak test, the modified rescaled range test and Lagrange multiplier type tests, exhibit size distortions in small samples. This paper investigates a parametric bootstrap testing procedure, for size correction, by means of a computer simulation study. The bootstrap provides a practical method to eliminate size distortions in the case of an asymptotic pivotal statistic while the power, in general, is close to the corresponding size adjusted asymptotic test. The results are very encouraging and suggest that a bootstrap testing procedure does correct for size distortions.
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| 3. |
- Andersson, Michael K.
(författare)
-
On testing and forecasting in fractionally integrated time series models
- 1998
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This volume contains five essays in the field of time series econometrics. All five discuss properties of fractionally integrated processes and models. The first essay, entitled Do Long-Memory Models have Long Memory?, demonstrates that fractional integration can enhance the memory of ARMA processes enormously. This is however not true for all combinations of diffe-rencing, autoregressive and moving average parameters. The second essay, with the title On the Effects of Imposing or Ignoring Long-Memory when Forecasting, investigates how the choice between mo-delling stationary time series as ARMA or ARFIMA processes affect the accu-racy of forecasts. The results suggest that ignoring long-memory is worse than imposing it and that the maximum likelihood estimator for the ARFIMA model is to prefer. The third essay, Power and Bias of Likelihood Based Inference in the Cointegration Model under Fractional Cointegration, investigates the performance of the usual cointegration approach when the processes are fractionally cointegrated. Under these circumstances, it is shown that the maximum likelihood estimates of the long-run relationship are severely biased. The fourth and fifth essay, entitled respectively Bootstrap Testing for Fractional Integration andRobust Testing for Fractional Integration using the Bootstrap, propose and investigate the performance of some bootstrap testing procedures for fractional integration. The results suggest that the empirical size of a bootstrap test is (almost) always close to the nominal, and that a well-designed bootstrap test is quite robust to deviations from standard assumptions.
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| 4. |
- Andersson, Michael K.
(författare)
-
On the effects of imposing or ignoring long memory when forecasting
- 1998
-
Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Since the true nature of a time series process is often unknown it is important to understand the effects of model choice. This paper examines how the choice between modelling stationary time series as ARMA or ARFIMA processes affects the accuracy of forecasts. This is done, for first-order autoregressions and moving averages and for ARFIMA(l,d,O) processes, by means of a Monte Carlo simulation study. The fractional models are estimated using the technique of Geweke and Porter-Hudak, the modified rescaled range and the maximum likelihood procedure. We conclude that ignoring long memory is worse than imposing it, when forecasting, and that the ML estimator is preferred.
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| 5. |
- Andersson, Michael K., et al.
(författare)
-
Power and bias of likelihood based inference in the cointegration model under fractional cointegration
- 1999
-
Ingår i: Economics Letters. - : Elsevier. - 0165-1765. ; 65:2, s. 143-147
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- This paper investigates how fractional cointegration affects the common maximum likelihood cointegration procedure. It is shown that the likelihood ratio test of no cointegration has considerable power against fractional alternatives. In contrast to the case of a cointegrated system, the usual maximum likelihood estimator gives severely biased estimates of the long-run relation under fractional cointegration. This suggests that the standard likelihood approach should be used with caution and that a test to separate fractionally cointegrated series from series that are cointegrated of an integer order should be executed prior to estimation.
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| 6. |
- Andersson, Michael K., et al.
(författare)
-
Robust testing for fractional integration using the bootstrap
- 1998
-
Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Asymptotic tests for fractional integration are usually badly sized in small samples, even for normally distributed processes. Furthermore, tests that are well-sized (under normality) may be seriously distorted by non-normalities and ARCH errors. This paper demonstrates how the bootstrap can be implemented to correct for such size distortions. It is shown that a well-designed bootstrap test based on the MRR and GPH tests is exact, and that a procedure based on the REG test is nearly exact. Ort, förlag, år, upplaga, sidor
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| 7. |
- Breimer, Michael, 1951, et al.
(författare)
-
Extracorporeal ("ex vivo") connection of pig kidneys to humans. I. Clinical data and studies of platelet destruction.
- 1996
-
Ingår i: Xenotransplantation. - : Wiley. - 0908-665X .- 1399-3089. ; 3:4, s. 328-39
-
Tidskriftsartikel (refereegranskat)abstract
- The pioneering experiment by Welsh et al. (Immunological Lett 1991:29:167-170) connecting a pig kidney to the human circulation has been repeated in a modified manner. Two volunteer dialysis patients were pretreated by daily plasmapheresis on days -2,-1, and 0 to remove the naturally occurring anti-pig xenoantibodies. The anti-pig lymphocytotoxic liters were reduced from 1:8 to 1:2 in patient 1 and from 1:8 to 1:1 in patient 2. No steroids or immunosuppressive drugs were administrated before or during the experiments. A sterile pig kidney was extracorporeally ("ex vivo") connected to the patients a/v fistula using an arterial and a venous pump similar to a dialysis. The two experiments gave different results. In the first experiment the perfusion pressure was kept at 100 mmHg for the initial 25 min by reducing the pump speed until the minimum blood flow of 30 ml/min was reached. Thereafter, the pressure rose continuously and the experiment was terminated at 65 min at a perfusion pressure of 200 mmHg. The patient did not feel any discomfort during the perfusion. In the second experiment, a stable blood flow of 200 ml/min was reached at a pressure of 100 mmHg after a few minutes. The perfusion was terminated at 15 min when the patient developed chest and abdominal pain, hypotension, and electrocardiographic signs of myocardial ischemia. The patient recovered quickly. In the first experiment, small volumes of clear urine was produced until the pressure rose above 100 mmHg, which resulted in hematuria. In the second experiment clear urine (4 ml/min) was produced. (51)Chromium clearance values were after 15 min <1 ml/min for kidney 1 and 12 ml/min (8 ml/min/100 g) for kidney 2. A drastic reduction in platelet count (128 to 48 and 64 to 8 × 10(9)/1, respectively) during the passage through the kidney was found in blood samples collected simultaneously before and after the organ. No change in hemoglobin values and leucocyte counts were found. Light- and electron-microscopical analysis of the kidney tissues revealed for kidney 1 focal areas with obliteration of the glomerular and peritubular capillaries by platelets and PMN cells and severe damage of the endothelial cells comparable to a picture of a hyperacute rejection. In kidney 2, all vessels were patent but in the capillaries large amount of membrane fragments were detected by electron microscopy and a discrete damage of the endothelial cells were seen in some segments. No intact platelets were present in the vascular tree. These human experiments support the hypothesis that hyperacute rejection of pig to human xenografts is delayed in time by removal of the preformed anti-pig xenoantibodies. A new finding was a very rapid destruction of platelets occurring in the kidney of patient 2 who had very low liters of xenoantibodies. The humoral immune response is described in detail in an accompanying paper (Rydberg et al., this issue).
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| 8. |
- Bremer, Kåre, et al.
(författare)
-
An ordinal classification for the families of flowering plants
- 1998
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Ingår i: ANNALS OF THE MISSOURI BOTANICAL GARDEN. - : MISSOURI BOTANICAL GARDEN. - 0026-6493. ; 85:4, s. 531-553
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Recent cladistic analyses are revealing the phylogeny of flowering plants in increasing detail, and there is support for the monophyly of many major groups above the family level. With many elements of the major branching sequence of phylogeny established
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| 9. |
- Kudryashov, V, et al.
(författare)
-
Characterization of a mouse monoclonal IgG3 antibody to the tumor-associated globo H structure produced by immunization with a synthetic glycoconjugate.
- 1998
-
Ingår i: Glycoconjugate journal. - 0282-0080. ; 15:3, s. 243-9
-
Tidskriftsartikel (refereegranskat)abstract
- Globo H (Fuc alpha1 --> 2Galbeta1 --> 3GalNAcbeta1 --> 3Gal alpha1 --> 4Galbeta1 --> 4Glc) is a carbohydrate structure that shows enhanced expression in many human carcinomas. From mice immunized with a globo H-KLH (keyhole limpet hemocyanin) synthetic conjugate an IgG3 monoclonal antibody (mAb VK-9) was derived that recognizes the globo H structure. Serological analysis showed that the minimal structure recognized by this mAb was the tetrasaccharide sequence Fuc alpha1 --> 2Galbeta1 --> 3GalNAcbeta1 --> 3Gal. An isomeric structure with an internal alphaGalNAc linkage was also recognized but less efficiently. mAb VK-9 did not react with many related structures, such as galactosylgloboside, globoside, H type 1, H type 2 blood group structures or fucosyl-gangliotetraosyl ceramide, but did react weakly with globo A ceramide. Not only did mAb VK-9 react with carbohydrate-protein conjugates but it could also recognize globo H-ceramide and human tumor cells expressing globo H. These results suggest that globo H-KLH could be explored as a vaccine in the treatment of carcinoma patients.
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| 10. |
- Lark, Michael W., et al.
(författare)
-
Aggrecan degradation in human cartilage : Evidence for both matrix metalloproteinase and aggrecanase activity in normal, osteoarthritic, and rheumatoid joints
- 1997
-
Ingår i: Journal of Clinical Investigation. - 0021-9738. ; 100:1, s. 93-106
-
Tidskriftsartikel (refereegranskat)abstract
- To examine the activity of matrix metalloproteinases (MMPs) and aggrecanase in control and diseased human articular cartilage, metabolic fragments of aggrecan were detected with monospecific antipeptide antibodies. The distribution and quantity of MMP-generated aggrecan G1 fragments terminating in VDIPEN341 were compared with the distribution of aggrecanase-generated G1 fragments terminating in NITEGE373. Both types of G1 fragments were isolated from osteoarthritic cartilage. The sizes were consistent with a single enzymatic cleavage in the interglobular domain region, with no further proteolytic processing of these fragments. Both neoepitopes were also detected by immunohistochemistry in articular cartilage from patients undergoing joint replacement for osteoarthritis (OA), rheumatoid arthritis (RA), and in cartilage from adults with no known joint disease. In control specimens, the staining intensity for both G1 fragments increased with age, with little staining in cartilage from 22-wk-old fetal samples. There was also an increase with age in the extracted amount of MMP- generated neoepitope in relation to both aggrecan and collagen content, confirming the immunohistochemical results. After the age of 20-30 yr this relationship remained at a steady state. The staining for the MMP-generated epitope was most marked in control cartilage exhibiting histological signs of damage, whereas intense staining for the aggrecanase-generated fragment was often noted in adult cartilage lacking overt histological damage. Intense staining for both neoepitopes appeared in the more severely fibrillated, superficial region of the tissue. Intense immunostaining for both VDIPEN- and NITEGE-neoepitopes was also detected in joint cartilage from patients with OA or RA. Cartilage in these specimens was significantly more degraded and high levels of staining for both epitopes was always seen in areas with extensive cartilage damage. The levels of extracted VDIPEN neoepitope relative to collagen or aggrecan in both OA and RA samples were similar to those seen in age-matched control specimens. Immunostaining for both types of aggrecan fragments was seen surrounding the cells but also further removed in the interterritorial matrix. In some regions of the tissue, both neoepitopes were found while in others only one was detected. Thus, generation and/or turnover of these specific catabolic aggrecan fragments is not necessarily coordinated. Our results are consistent with the presence in both normal and arthritic joint cartilage of proteolytic activity against aggrecan based on both classical MMPs and 'aggrecanase'.
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| 11. |
- Lark, Michael W., et al.
(författare)
-
Aggrecan degradation in osteoarthritis and rheumatoid arthritis
- 1995
-
Ingår i: Acta Orthopaedica. - : Informa UK Limited. - 1745-3674 .- 0001-6470. ; 66:S266, s. 92-97
-
Tidskriftsartikel (refereegranskat)abstract
- Aggrecan turnover is critically important to maintain extracellular matrix homeostasis in articular cartilage. Cartilage aggrecan metabolism has been studied in chondrocyte cell cultures, cartilage explant cultures, and in whole animal models. In many of these studies, matrix metalloproteinases (MMPs) are proposed to degrade cartilage aggrecan. MMP expression appears elevated in joint tissues from patients with osteoarthritis (OA) and rheumatoid arthritis (RA) (Dean et al. 1989, Wolfe et al. 1993) and inhibitors of both MMPs and thiol proteinases have been shown to block aggrecan (Buttle et al. 1992) and type II collagen (Mort et al. 1993) degradation in cartilage explant cultures. Using antibodies and cDNA probes, elevations in expression and concentration of many of these enzymes in animal models and in OA and RA have been described. Human cartilage aggrecan has now been cloned and sequenced (Doege et al. 1991). This information, in combination with the ability to sequence aggrecan and aggrecan fragments at the protein level, has resulted in the identification of several aggrecan fragments which appear to result from proteinase degradation. In this report, we describe data which suggest that MMPs may in part be responsible for aggrecan catabolism in normal articular cartilage, as well as in the elevated catabolism seen in OA and RA.
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| 12. |
- Lindholm, Cecilia K, et al.
(författare)
-
Requirement of the Src homology 2 domain protein Shb for T cell receptor-dependent activation of the Interleukin-2 gene nuclear factor for activation of T cells element in Jurkat T cells
- 1999
-
Ingår i: Journal of Biological Chemistry. - : Elsevier BV. - 0021-9258 .- 1083-351X. ; 274:39, s. 28050-28057
-
Tidskriftsartikel (refereegranskat)abstract
- Stimulation of the T cell antigen receptor (TCR) induces tyrosine phosphorylation of numerous intracellular proteins. We have recently investigated the role of the adaptor protein Shb in the early events of T cell signaling and observed that Shb associates with Grb2, linker for activation of T cells (LAT) and the TCR zeta-chain in Jurkat cells. We now report that Shb also associates with phospholipase C-gamma1 (PLC-gamma1) in these cells. Overexpression of Src homology 2 domain defective Shb caused diminished phosphorylation of LAT and consequently the activation of mitogen-activated protein kinases was decreased upon TCR stimulation. In addition, the Shb mutant also blocked phosphorylation of PLC-gamma1 and the increase in cytoplasmic Ca(2+) following TCR stimulation. Nuclear factor for activation of T cells is a major target for Ras and calcium signaling pathways in T cells following TCR stimulation, and the overexpression of the mutant Shb prevented TCR-dependent activation of the nuclear factor for activation of T cells. Consequently, endogenous interleukin-2 production was decreased under these conditions. The results indicate a role for Shb as a link between the TCR and downstream signaling events involving LAT and PLC-gamma1 and resulting in the activation of transcription of the interleukin-2 gene.
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| 13. |
- Lindström, K, et al.
(författare)
-
Blood group glycosphingolipid expression in kidney of an individual with the rare blood group A1 Le(a-b+) p phenotype: absence of blood group structures based on the globoseries.
- 1996
-
Ingår i: Glycoconjugate journal. - 0282-0080. ; 13:2, s. 307-13
-
Tidskriftsartikel (refereegranskat)abstract
- Total neutral glycolipid fractions were isolated from kidney and ureter tissue obtained at autopsy of an individual of the rare blood group A1 Le(a-b+) p. The amount of glycolipids isolated were 3.7 and 2.5 mg g-1 dry tissue weight for the kidney and ureter tissue, which is in the range of reference blood group P kidneys. Part of the kidney glycolipid fraction was subfractionated by HPLC. Glycolipid compounds were structurally characterized by thin-layer chromatography (chemical detection and immunostaining with monoclonal antibodies), proton NMR spectroscopy and mass spectrometry. Globotriaosyl- and globotetraosyl-ceramides, which are the major compounds in kidneys of P individuals, were absent in the p kidney, and a comparatively increased amount of monoglycosyl- and lactosylceramides was found. A shift to longer fatty acyl chains in the ceramide part of lactosylceramides was noted. Elongated globoseries compounds with five to seven sugar residues, including the blood group A type 4 chain structure, were lacking. A slight increase in neolactotetraosyl- and blood group X pentaglycosyl-ceramides was noticed. The study confirms an enzymatic block in the conversion of lactosylceramide to elongated globoseries compounds in the kidney tissue similar to that of erythrocytes of p individuals.
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| 14. |
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| 15. |
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| 16. |
- Matsui, S, et al.
(författare)
-
Active immunization of combined beta1-adrenoceptor and M2-muscarinic receptor peptides induces cardiac hypertrophy in rabbits.
- 1999
-
Ingår i: Journal of cardiac failure. - 1071-9164. ; 5:3, s. 246-54
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: The high prevalence of patients with dilated cardiomyopathy (DCM) with anti-beta1-adrenoceptor and/or anti-M2-muscarinic receptor autoantibodies in their sera has been observed. However, the pathophysiological role of these autoantibodies in the development of cardiomyopathy is unknown. We previously reported an experimental model of early-stage DCM-like cardiomyopathy induced by immunizing rabbits for 1 year with synthetic peptides corresponding to the sequence of the second extracellular loop of either beta1-adrenoceptor or M2-muscarinic receptor. Because approximately half the sera of patients with DCM that recognize one of the two receptor sequences also recognize the second sequence, a model was created in rabbits simultaneously immunized with the synthetic peptides corresponding to the second extracellular loop of the beta1-adrenoceptor and M2-muscarinic receptor. METHODS AND RESULTS: All rabbits (n = 8) immunized with both peptides had a high titer of both anti-beta1-adrenoceptor and anti-M2-muscarinic receptor autoantibodies in their sera, whereas none of the sera from control rabbits injected with saline (n = 9) was positive. No significant cross-reaction with peptides other than those used for immunization was found. The weight of the hearts of immunized rabbits increased significantly. The hearts of immunized rabbits showed marked concentric left ventricular hypertrophy with mild inflammatory cell infiltration. In these rabbits, mild or moderate interstitial fibrosis was also observed. In electron micrographs, immunized rabbits showed focal myofibrillar lysis, loss of myofilament, and a marked increase in the number of mitochondria and deposition of dense granules in both sarcoplasm and myofibrils. Conversely, one of the control rabbits showed scant mononuclear cell infiltration. However, in this control rabbit, no significant alteration was found by electron microscopy. CONCLUSION: Our results showed the coexistence of both anti-beta1-adrenoceptor and anti-M2-muscarinic receptor autoantibodies in the sera has pathophysiological importance, shown by their ability to induce cardiac hypertrophy in rabbits.
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| 17. |
- Matsui, S, et al.
(författare)
-
Peptides derived from cardiovascular G-protein-coupled receptors induce morphological cardiomyopathic changes in immunized rabbits.
- 1997
-
Ingår i: Journal of molecular and cellular cardiology. - : Elsevier BV. - 0022-2828. ; 29:2, s. 641-55
-
Tidskriftsartikel (refereegranskat)abstract
- An experimental model of early-stage cardiomyopathy was created by immunizing rabbits for 1 year with synthetic peptides corresponding to the sequence of the second extracellular loop of either beta-adrenoceptors or M2-muscarinic receptors. Thirty male rabbits were used and divided into three groups: a control group (n = 10), a group immunized with the peptide corresponding to the beta-adrenoceptor (beta 1 group) (n = 10) and a group immunized with the peptide corresponding to the M2-muscarinic receptor (M2 group) (n = 10). If the sera from both groups of immunized rabbits high-titres of anti-peptide antibodies were found throughout the study period but not in the sera from control rabbits or in the preimmune sera of immunized rabbits. No significant cross-reaction with peptides other than those used for immunization was found. The myocardial receptor density of both immunized groups displayed a strong trend toward receptor up-regulation. This was significant in the beta 1 group but not in the M2 group. Both groups of immunized rabbits displayed significantly enlarged ventricles and thinner walls, as compared with the control group. However, in contrast to the beta 1 group, which showed enlarged cavities in both left and right ventricles, the M2 group was mainly affected in the right ventricles. Moreover, morphological examinations of the hearts of rabbits from both immunized groups demonstrated focal myofibrillar lysis, loss of myofilament, mitochondrial swelling and condensation, sarcoplasmic vacuolation, deposition of dense granules in the sarcoplasm and the myofibrils. One of the sex control rabbit hearts which were examined showed mild degenerative changes in the myocardium and scant mononuclear cell infiltration. However, when all the control rabbit hearts were examined by electron microscopy, no significant alterations were found. These results suggest that immunization by peptides, corresponding to the target sequences for anti-receptor autoantibodies in idiopathic dilated cardiomyopathy, induces morphological changes in the heart similar to those found in the human disease.
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| 18. |
- Ménoret, A, et al.
(författare)
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The expression of carbohydrate blood group antigens correlates with heat resistance.
- 1995
-
Ingår i: Journal of cell science. - 0021-9533. ; 108 ( Pt 4), s. 1691-701
-
Tidskriftsartikel (refereegranskat)abstract
- Recent data indicate that cells may resist heat shock via more than one route: heat shock protein synthesis and other still ill-defined mechanisms. We investigated this phenomenon using four types of cells derived from a single rat colon carcinoma: clones REGb and PROb; PRO A+, a glycosylation variant of PROb selected for its high expression of blood group A antigen; and Ph8, a thermoresistant variant of PROb selected by repeated sublethal heat treatments. Basal heat resistance was clearly associated with the level of cell surface expression of blood group H and A antigens. Biosynthesis of these carbohydrate structures requires two glycosyltransferases, H and A enzymes, whose activities are also correlated with basal heat resistance. In addition, heat sensitive REGb cells were rendered more resistant by transfection with the gene encoding for H enzyme, allowing expression of H antigen. Thus, these terminal glycosylations could play a role as cellular protectors against heat treatment. Blood group carbohydrate antigens were mainly located on O-linked carbohydrate chains of a major glycoprotein of 200 kDa and to a lesser extent on N-linked chains. Only trace amounts were present as glycolipids.
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| 19. |
- Peukert, S, et al.
(författare)
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The frequency of occurrence of anti-cardiac receptor autoantibodies and their correlation with clinical manifestation in patients with hypertrophic cardiomyopathy.
- 1999
-
Ingår i: Autoimmunity. - 0891-6934. ; 29:4, s. 291-7
-
Tidskriftsartikel (refereegranskat)abstract
- The purpose of this study was to investigate the frequency of occurrence of autoantibodies against G-protein coupled cardiovascular receptors and their relation to the clinical manifestation of hypertrophic cardiomyopathy (HCM). Autoantibodies against beta1-receptors, Muscarin-2-receptors, Angiotensin-II-receptor subtype 1 and alpha1-receptors were determined with ELISA in 52 patients with HCM (37 male, 15 female, mean age 55 +/- 15 years) and 40 healthy, age and sex matched controls. The clinical characterization of the HCM-patients included ECG, 24-h Holter, and echocardiography. The results showed that there is no significant difference in the frequency of a single autoantibody between HCM-patients and controls. However, if the number of patients who have autoantibodies against beta1-receptors and/or Muscarin-2-receptors were counted together, there are significantly more autoantibodies in HCM compared to controls (11 vs. 2, p = 0.035). Analysis of clinical data from this pooled group of patients showed that in patients with autoantibodies, heart rate variability (HRV), ultra low frequency (ULF) and very low frequency (VLF) were decreased (HRV by 20%, ULF by 50%, and VLF by 46%, p < 0.008) whereas the QTc-interval was increased by 8% (p < 0.02 each). The ratio of septal to posterior wall thickness was increased by 23% (p = 0.05), and the preejection period was prolonged by 46% in patients with autoantibodies (p < 0.001). These results suggest that the existence of these autoantibodies could be associated with an advanced stage or a severe manifestation of HCM.
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| 20. |
- Rogers, David, et al.
(författare)
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Highlights of Coastal Waves 1996
- 1998
-
Ingår i: Bulletin of The American Meteorological Society - (BAMS). - 0003-0007 .- 1520-0477. ; 79, s. 1307-1326
-
Tidskriftsartikel (refereegranskat)
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| 21. |
- Roth, Michael, et al.
(författare)
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Guided waves in near-surface seismic surveys
- 1998
-
Ingår i: Geophysical Research Letters. - : American Geophysical Union (AGU). - 0094-8276 .- 1944-8007. ; 25:7, s. 1071-1074
-
Tidskriftsartikel (refereegranskat)
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| 22. |
- Rydberg, Lennart, 1944, et al.
(författare)
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An ELISA technique for quantitation of human xenoantibodies binding to pig cells: application in patients with pig kidneys extracorporeally connected to the circulation.
- 1998
-
Ingår i: Xenotransplantation. - 0908-665X. ; 5:2, s. 105-10
-
Tidskriftsartikel (refereegranskat)abstract
- A quantitative ELISA technique for determination of human anti-pig xenoantibody number in serum samples has been established using pig lymphocytes and pig/rabbit erythrocytes as target cells and a pool of serum from human blood group AB donors. The number of low affinity antibodies binding to the cells was determined by quantitation following the use of aqueous washing of the cells and separation of bound and unbound antibodies with the phthalate oil method. The efficiency of different soluble Gal(alpha)1-3Gal-terminating di- and tri-saccharides to inhibit antibody binding was tested and found to vary between 70-90% at a saccharide concentration of 10 mg/ml. The assay was used to evaluate the antibody changes in two patients who, after plasmapheresis treatments, had pig kidneys extracorporeally connected to their blood circulation. The number of anti-pig IgM/IgG antibodies bound to each pig lymphocyte were reduced from 5,600/13,200 to 1,300/3,100 in patient 1 and from 1,200/6,500 to 500/2,100 in patient 2 by three consecutive daily plasmapheresis treatments. Although the lymphocytotoxic titers were reduced to very low levels, the antibody numbers still present in the blood of patient 1 caused a hyperacute rejection of the pig kidney. However, the antibody levels in patient 2 did not cause rejection of this kidney during 15 min perfusion time. A strong anti-pig antibody response 3 weeks after the perfusion experiment was found in patient 1 as shown by 27,600/245,300 IgM/IgG molecules bound to pig lymphocytes corresponding to an increase of lymphocytotoxic titer from 8 to 512. The second patient showed a much weaker immune response with 1,400/19,800 IgM/IgG antibodies corresponding to a lymphocytotoxic titer increase from 8 to 32. The use of this quantitation technique enables more accurate investigation of antibody binding to xenogenic target cells than conventional titration techniques.
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| 23. |
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| 24. |
- Thorell, Anders, et al.
(författare)
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Surgery-induced insulin resistance in human patients relations to glucoseutilization and transport
- 1999
-
Ingår i: American Journal of Physiology. - : American Physiological Society. - 0002-9513 .- 2163-5773. ; 276:4, s. E754-E761
-
Tidskriftsartikel (refereegranskat)abstract
- To investigate the underlying molecular mechanisms for surgery-induced insulin resistance in skeletal muscle, six otherwise healthy patients undergoing total hip replacement were studied before, during, and after surgery. Patients were studied under basal conditions and during physiological hyperinsulinemia (60 microU/ml). Biopsies of vastus lateralis muscle were used to measure GLUT-4 translocation, glucose transport, and glycogen synthase activities. Surgery reduced insulin-stimulated glucose disposal (P < 0.05) without altering the insulin-stimulated increase in glucose oxidation or suppression of endogenous glucose production. Preoperatively, insulin infusion increased plasma membrane GLUT-4 in all six subjects (P < 0.05), whereas insulin-stimulated GLUT-4 translocation only occurred in three patientspostoperatively (not significant). Moreover, nonoxidative glucose disposal rates and basal levels of glycogen synthase activities in muscle were reduced postoperatively (P < 0.05). These findings demonstrate that peripheral insulin resistance develops immediately postoperatively and that this condition might be associated with perturbations in insulin-stimulated GLUT-4 translocation as well as nonoxidative glucose disposal, presumably at the level of glycogen synthesis.
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| 25. |
- Tulldahl, H. Michael, et al.
(författare)
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Analytical waveform generation from small objects in lidar bathymetry
- 1999
-
Ingår i: Applied Optics. - 0003-6935 .- 1539-4522. ; 38:6, s. 1021-1039
-
Tidskriftsartikel (refereegranskat)abstract
- We present a model to simulate receiver waveforms from an airborne sea-depth-sounding lidar to compare the influence that is due to different shapes of objects placed on the sea bottom. The objects are of size 1 m(3), and the bottom depths are 5-12 m. We use an existing analytical beam-propagation model and divide the bottom into squares. For each element on the bottom grid we create a transmitted and a reflected waveform. The waveforms are summed, yielding a total contribution from all bottom elements. We compare two object types, cylinder and cube, and find that the difference in the receiver waveform is small between these objects. Simulated waveforms are compared with experimental data from the Swedish Hawk Eye system and show good agreement.
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| 26. |
- Wilstermann, Michael, et al.
(författare)
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Synthesis of Ganglioside Lactams Corresponding to Gm1-, Gm2-,Gm3-, and GM4-Ganglioside Lactones
- 1995
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 117:17, s. 4742-4754
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Tidskriftsartikel (refereegranskat)abstract
- Ganglioside lactams are potentially useful analogs of ganglioside lactones, which are highly immunogenic derivatives of gangliosides. The lactam corresponding to the GM3-lactone saccharide has been synthesized by sialylation of a suitably protected lactose derivative carrying an azido group in the 2'-position, followed by reduction and ring closure to form GM3-lactam. Glycosylation in the 4-position of the central saccharide unit gave the Gm2- and Gm1-lactam saccharides. By a similar route, a 2-azido-Gal derivative was sialylated and treated as above to give the GM4-lactam saccharide. Deprotection gave the GM2-4-lactam saccharides in water soluble form, whereas attempted deprotection of the Gm1-lactam caused its degradation. The GM3-lactam saccharide was coupled to ceramide, to afford the ganglioside lactam analog, and via a spacer to bovine serum albumin (BSA). The BSA conjugate was used as immunogen to raise monoclonal antibodies that cross-reacted with GM3-lactone. The antibodies were used in a histological staining of murine melanoma cells, clearly showing the presence of GM3-lactone on the cell surface. Keeping the GM2-4-lactam saccharides in D2O at 37 °C for 1 month caused marginal (0—11%) hydrolysis of the lactam ring.
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| 27. |
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| 28. |
- Xing, K. Z., et al.
(författare)
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The electronic and geometric structures of neutral and potassium-doped poly[3-(4-octylphenyl)thiophene] studied by photoelectron spectroscopy
- 1996
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Ingår i: Synthetic metals. - : Elsevier. - 0379-6779 .- 1879-3290. ; 76:1-3, s. 263-267
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Tidskriftsartikel (refereegranskat)abstract
- The electronic and geometric structures of poly [3-(4-octylphenyl)thiophene] have been studied by X-ray and ultraviolet photoelectron spectroscopy (XPS and UPS, respectively). Thermochromic effects, and new charge induced states generated by potassium doping, have been observed by direct UPS measurements. The experimental results are in very good agreement with the results of theoretical quantum chemical calculations performed with the Austin Model 1 semi-empirical model and the valence-effective Hamiltonian pseudo-potential model.
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| 29. |
- Xing, K. Z., et al.
(författare)
-
The electronic structure of neutral and alkali metal-doped poly[3-(4-octylphenyl)thiophene] studied by photoelectron spectroscopy
- 1996
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Ingår i: Synthetic metals. - : Elsevier. - 0379-6779 .- 1879-3290. ; 80:1, s. 59-66
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Tidskriftsartikel (refereegranskat)abstract
- The electronic structure of poly [3-(4-octylphenyl)thiophene] (POPT) has been studied by ultraviolet photoelectron spectroscopy (UPS) and X-ray photoelectron spectroscopy (XPS), as well as by quantum chemical calculations. Both temperature-dependent effects on the electronic structure of the neutral system, as well as the generation of new electronic states induced by doping with alkaline metals, have been observed. The experimental results are in good agreement with the results of the quantum chemical calculations.
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