| 1. |
- Bunk, Richard, et al.
(författare)
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Guiding molecular motors with nano-imprinted structures
- 2005
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Ingår i: Japanese Journal of Applied Physics. - 0021-4922. ; 44:5A, s. 3337-3340
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Tidskriftsartikel (refereegranskat)abstract
- This work, for the first time, demonstrates that nano-imprinted samples, with 100 nm wide polymer lines, can act as guides for molecular motors consisting of motor proteins actin and myosin. The motor protein function was characterized using fluorescence microscopy and compared to actomyosin motility on non-structured nitrocellulose surfaces. Our results open for further use of the nano-imprint technique in the production of disposable chips for bio-nanotechnological applications and miniaturized biological test systems. We discuss how the nano-imprinted motor protein assay system may be optimized and also how it compares to previously tested assay systems involving low-resolution UV-lithography and low throughput but high-resolution electron beam lithography.
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| 2. |
- Bunk, Richard, et al.
(författare)
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Guiding motor-propelled molecules with nanoscale precision through silanized bi-channel structures
- 2005
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Ingår i: Nanotechnology. - : IOP Publishing. - 0957-4484 .- 1361-6528. ; 16:6, s. 710-717
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Tidskriftsartikel (refereegranskat)abstract
- We report on the design and fabrication of a channel structure for high precision guidance and achieving excellent confinement properties for motor-propelled molecular shuttles. The techniques used to manufacture the channel structure are mainly e-beam lithography and selective monolayer functionalization. The structure consists of two lateral layers of concentric channels on a SiO2 surface made biocompatible with the molecular motors. The quality and advantages of the design are demonstrated by experiments using the motor proteins actin and myosin. The special channel geometry leads to stable biochemical conditions with full motor protein functionality. ATP is sufficiently supplied to all parts of the structure by dedicated service channels, as is the venting of ADP and P-i (inorganic phosphorus). Channels of different widths (100-700 nm) and shapes are fabricated and measurements made on them.
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| 3. |
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| 4. |
- Gourgon, C., et al.
(författare)
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Benchmarking of 50 nm features in thermal nanoimprint
- 2007
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Ingår i: Journal of Vacuum Science and Technology B. - : American Vacuum Society. - 1520-8567. ; 25:6, s. 2373-2378
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Tidskriftsartikel (refereegranskat)abstract
- The objective of this benchmarking is to establish a comparison of several tools and processes used in thermal NIL with Si stamps at the nanoscale among the authors' laboratories. The Si stamps have large arrays of 50 nm dense lines and were imprinted in all these laboratories in a similar to 100 nm thick mr-18010E film. Other materials, such as mr-17010E, were also tested. Good patterns were obtained and some limitations were identified. Reducing the pressure to 15 bars enables the printing of 50 nm structures without pulling them off. At higher pressures, some bending effects resulting in pattern deformation were observed. It was proven that a pressure of 1.5 bars is sufficient to imprint perfect 50 nm lines. The influence of the antiadhesive layer and mold design has been characterized by the demonstration of pulled off lines in some cases. Moreover, it has been shown that the scatterometry method is particularly useful for the characterization of 50 nm lines and that the residual layer thickness corresponds to the theoretical estimate as long as the lines are well defined. One process was demonstrated which combines high reproducibility with high throughput, achieving a cycle time of 2 min. (c) 2007 American Vacuum Society.
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| 5. |
- Jensenius, M, et al.
(författare)
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Scrub typhus imported to Scandinavia
- 2006
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Ingår i: Scandinavian journal of infectious diseases. - : Informa UK Limited. - 0036-5548 .- 1651-1980. ; 38:3, s. 200-202
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Tidskriftsartikel (refereegranskat)
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| 6. |
- Koksharov, Yu.A., et al.
(författare)
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Magnetostatic interactions in planar ring-like nanoparticle structures
- 2006
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Ingår i: Thin Solid Films. - : Elsevier BV. - 0040-6090. ; 515, s. 731-734
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Tidskriftsartikel (refereegranskat)abstract
- Numerical calculations of equilibrium state energies and local magnetic fields in planar ring-like nanoparticle structures were performed. The dipole–dipole, Zeeman and magnetic anisotropy interactions were included into the model. The result of their competition depends on the value of the external magnetic field, magnetic parameters of an individual nanoparticle, size and shape of the structures. Flux-closed vortexes, single domain, two- domain ‘‘onion’’-like, ‘‘hedgehog’’-like and more complex spin structures can be realized. The critical field, providing a sharp transition from the flux-closed vortex to the ‘‘onion’’-like state, can be regulated by a variation of the particle magnetization and anisotropy constant, their easy directions, and particle space arrangement.
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| 7. |
- Mansson, A, et al.
(författare)
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Actin-based molecular motors for cargo transportation in nanotechnology - Potentials and challenges
- 2005
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Ingår i: IEEE Transactions on Advanced Packaging. - 1521-3323. ; 28:4, s. 547-555
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Tidskriftsartikel (refereegranskat)abstract
- Here, we review the use of actin-based motors, (myosins; e.g., the molecular motor of muscle) in. nanotechnology. The review starts from the viewpoints of the molecular motors as being important devices responsible of cargo transportation in the cell and end in discussions about their employment in nanotechnological applications. First, we describe basic biophysics of the myosin motors with focus on their involvement in cargo transportation in the living cell, leading us over into a discussion about in vitro motility assays. These are biological test systems where the myosin-induced translocation of actin filaments is studied on an artificial surface outside the cell. Then follows a review about modified motility assays for production of ordered motion. Here, we discuss ours and others' work with regards to making micro- and nanostructured surfaces and channels where the position and direction of movement produced by molecular motors is controlled. In this section, we consider the role of the channel size in promoting unidirectional myosin-induced motion of actin filaments. Furthermore, we consider the usefulness of surface modifications, e.g., various silanization procedures in order to promote and hinder molecular motility, respectively. Particularly, we describe our latest test system being both morphologically and chemically nanostructured giving us unsurpassed possibilities to perform functional studies as well as extremely good spatio-temporal control. Then follows a section about nanotechnological cargo transportation systems based on the actomyosin motor system. For instance, we present results of attaching fluorescent quantum dots as cargoes to the actin filaments. In this section, we also discuss the possibilities of having cargo attachment and detachment being performed on demand. Finally, we consider the usefulness of molecular motors for lab-on-a-chip applications and the requirements for incorporating these motors in commercially viable devices. In this context, the significant potential of the actomyosin motor system to overcome traditional limitations of micro- and nanofluidics is stressed.
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