| 1. |
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| 2. |
- Adiels, Lars, 1952-, et al.
(författare)
-
Test of CP violation with K0 and K‾0 at LEAR
- 1985
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Ingår i: Physics with antiprotons at LEAR in the ACOL era. - Gif sur Yvette : Editions Frontières. - 2863320351 ; , s. 467-482
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 3. |
- Köhler, Th., et al.
(författare)
-
Precision measurement of strong interaction isotope effects in antiprotonic 16O, 17O, and 18O atoms
- 1986
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Ingår i: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 176:3-4, s. 327-333
-
Tidskriftsartikel (refereegranskat)abstract
- The strong-interaction effects in antiprotonic 16O, 17O, and 18O atoms were measured at the CERN antiproton facility, LEAR. The shifts ε{lunate} and the widths Γ of the 3d level were determined to be -112±20 eV (16O), -140±46 eV (17O), -195±20 eV (18O), and 495±45 eV (16O), 540±150 (17O), 640±40 eV (18O), respectively. © 1986.
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| 4. |
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| 5. |
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| 6. |
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| 7. |
- Nilsson, Bo, et al.
(författare)
-
Distinctive expression of neoantigenic C3(D) epitopes on bound C3 following activation and binding to different target surfaces in normal and pathological human sera
- 1989
-
Ingår i: Molecular Immunology. - : Elsevier BV. - 0161-5890 .- 1872-9142. ; 26:4, s. 383-390
-
Tidskriftsartikel (refereegranskat)abstract
- Binding of C3 to sheep erythrocytes in a serum-free milieu (EAC14oxy2, EAC142) has previously been shown to mimic the antigenic change that occurs upon denaturation of C3 in sodium dodecyl sulphate (SDS), whereby neoantigenic C3(D) epitopes are exposed. The present paper deals with C3 bound to various target surfaces which are known to modulate the functional properties of C3 in different ways. Bound C3 fragments on serum-treated human aggregated gammaglobulin, zymosan, rabbit and sheep erythrocytes, and on circulating immune complexes isolated from sera of patients with rheumatoid arthritis and systemic lupus erythematosus, were shown to be mainly in the iC3b form. By RIAs, employing polyclonal antibodies, the range of C3(D) antigenic epitopes of 125I-labelled SDS denatured C3 expressed by the particle-bound iC3b was monitored. The physiologically bound iC3b on all tested particles expressed wide ranges of C3(D) epitopes and each type of particle-bound C3 exposed its individual range. By competition ELISA specific C3(D)α epitopes were monitored, employing monoclonal antibodies. A distinct difference in the expression of these epitopes was observed in iC3b bound to various test particles in the presence of normal serum and in iC3b present on circulating immune complexes from pathological sera. Considering that the neoantigenic C3(D) epitopes have been shown to be associated with different functions of C3, the distinctive antigenic expression of each type of serum-treated particle might reflect different functional forms of the protein.
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| 8. |
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| 9. |
- Dotevall, A, et al.
(författare)
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Platelet reactivity, fibrinogen and smoking.
- 1987
-
Ingår i: European journal of haematology. - 0902-4441. ; 38:1, s. 55-9
-
Tidskriftsartikel (refereegranskat)abstract
- 40 young healthy male volunteers (20 habitual smokers and 20 non-smokers) were investigated with respect to platelet reactivity, plasma fibrinogen and coagulation factor VIII. Smokers had significantly lower systolic blood pressures and higher venous platelet counts. The results for ADP-induced platelet aggregation, plasma concentrations for the 2 alpha-granule proteins, beta-thromboglobulin and platelet factor 4, did not differ between the 2 study groups involved; nor was there any difference between serum thromboxane B2 formation or plasma factor VIII:C activity. However, as compared to non-smokers, plasma fibrinogen levels were significantly higher among the smokers.
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| 10. |
- Lacy, R. A, et al.
(författare)
-
Photoionization-pumped gain at 185 nm in a laser-ablated indium plasma
- 1989
-
Ingår i: Journal of the Optical Society of America B: Optical Physics. - 0740-3224. ; 6:6, s. 1209-1216
-
Tidskriftsartikel (refereegranskat)abstract
- We reportwhat is to our knowledge the first production of gain by inner-shell photoionization of ions. Photoionization of 4d electrons from In$+$ ions created a population inversion on a 185-nm In2$+$ transition. This scheme is an isoelectronic scaling of the 442-nm Cd$+$ laser. A single-pass gain constant of 0.9 {\textpm} 0.3 cm{\textminus}1 over a 0.4-cm path was pumped by soft x rays generated by less than 50 mJ of 1.06-$\mu$m light focused onto a tantalum target. Laser ablation of a liquid-indium target provided a high density (1016 cm{\textminus}3) of ground-state In$+$ ions for this scheme. An indium amplified spontaneous emission laser and a synchronously pumped indium laser oscillator are proposed.
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| 11. |
- Levin, J.C., et al.
(författare)
-
Production of very cold, highly charged ions by synchrotron radiation: Comparisons of the “scalpel” and “hammer” methods
- 1987
-
Ingår i: Nuclear Instruments & Methods in Physics Research. Section A: Accelerators, Spectrometers, Detectors, and Associated Equipment. - : Elsevier BV. - 0168-9002. ; 262:1, s. 106-109
-
Tidskriftsartikel (refereegranskat)abstract
- Measurements of kinetic energies of highy charged argon ions produced by inner-shell photoionization and by ion-beam impact have been made using time-of-flight techniques. High-charge-state recoil ions produced by beams of ∼ 0.5-1 MeV/u Cl5+ are found to have energies one to two orders of magnitude higher than ions of the same charge produced by vacancy cascades following inner-shell photoionization by synchrotron radiation. The results may have application to the development of a very cold ion source useful for angle-resolved atomic collision studies.
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| 12. |
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| 13. |
- Malmvall, B E, et al.
(författare)
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Immune complexes, rheumatoid factors, and cellular immunological parameters in patients with giant cell arteritis
- 1981
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Ingår i: Annals of the Rheumatic Diseases. - 1468-2060. ; 40:3, s. 276-280
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Tidskriftsartikel (refereegranskat)abstract
- Circulating immune complexes were found in 2 of 15 patients with giant cell arteritis (GCA) by using a solid phase Clq enzyme linked immunoabsorbent assay (ELISA). The prevalence in controls was 5%. Rheumatoid factor could be demonstrated in 2 out of 27 patients and in 11% of the controls by using a similar ELISA technique. The prevalence of T cells in blood was similar in 25 patients with GCA and in controls. The blood lymphocyte blastogenic response to the mitogens, phytohaemagglutinin, concanavalin-A, and pokeweed mitogen did not differ in 25 untreated patients compared with controls. Stimulation of lymphocytes by arterial homogenates was tested in 8 patients. In no case could a significant simulation by obtained. We conclude that immune complexes and rheumatoid factors are present in the same low frequency in GCA patients as in the normal population, and that the studied parameters of cellular immunity appear to be normal.
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| 14. |
- Nilsson, B, et al.
(författare)
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Quantitation and antigenic characterization of bound C3 of circulating immune complexes in systemic lupus erythematosus, rheumatoid arthritis, and primary biliary cirrhosis.
- 1987
-
Ingår i: Journal of Clinical Immunology. - 0271-9142 .- 1573-2592. ; 7:5, s. 420-426
-
Tidskriftsartikel (refereegranskat)abstract
- In recent years defective function of the complement-mediated clearance of immune complexes (IC) has been reported in patients with immune complex disease. The defect has been found at different levels in the clearance system. An important event in this sequential system is the binding of C3-coated particles to C3 receptors on erythrocytes and phagocytes. This study focuses on immunochemical properties of IC-bound C3 that reflect the functional state of the molecule. Sera from patients with primary biliary cirrhosis (PBC), rheumatoid arthritis (RA), and systemic lupus erythematosus (SLE) and from normal subjects were analyzed for their level of C3 precipitable in 2.7% (w/v) polyethylene glycol (PEG). The mean levels for the patient categories were significantly higher than that for the normal subjects. The immunochemical study revealed several differences among the different forms of PEG-precipitable C3. All forms expressed C3(D) antigens which are expressed by immune complex-associated and denatured forms but not by soluble physiological forms of C3. The expression of these antigens was proportionately lower for the complex-associated C3 of PBC compared to that of RA and SLE. Furthermore, employing monoclonal anti-C3(D) antibodies against the C3c and the C3d domain, distinct differences could be detected among all forms of PEG-precipitable C3. Sera from RA and SLE, in particular, contained PEG-precipitable C3 that exhibited distinctive immunochemical features with respect to these epitopes.
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| 15. |
- Nilsson, O G, et al.
(författare)
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Combined cholinergic and serotonergic denervation of the forebrain produces severe deficits in a spatial learning task in the rat
- 1988
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Ingår i: Brain Research. - : Elsevier BV. - 0006-8993. ; 453:1-2, s. 235-246
-
Tidskriftsartikel (refereegranskat)abstract
- The purpose of the present experiments was to study the effects of a combined cholinergic and serotonergic denervation of the rat forebrain on spatial learning using the Morris water maze task. Experiment 1 compared the acute effects of a radiofrequency lesion of the septum, an intraventricular 5,7-dihydroxytryptamine (5,7-DHT) lesion, and a combined septal plus 5,7-DHT lesion. Although the 5,7-DHT lesion alone did not produce any significant deficits in the water maze task, the lesion greatly potentiated the learning impairments produced by the septal lesion. Thus, the rats with both lesions combined showed severe difficulties in finding the platform and they did not develop any place navigational search strategy. This effect was not dependent on any effect on swimming ability or locomotor activity. The long-term effects of the combined septal and 5,7-DHT lesion was investigated in experiment 2, where the rats were tested in the water maze both 5 and 24-25 weeks after surgery. In this experiment, the rats showed the same severe deficits in spatial learning in both tests, showing that the impairments remain for long periods and after extended training. The results show that a combination of a cholinergic and a serotonergic denervation of the rat forebrain produces pronounced impairments in spatial learning in the Morris water maze task, and that this effect is long-lasting. This indicates that the recently proposed serotonergic deficit in patients with Alzheimer's disease may contribute importantly to the cognitive disabilities in these patients.
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| 16. |
- Pellettieri, L, et al.
(författare)
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Serum immunocomplexes in patients with subarachnoid hemorrhage.
- 1986
-
Ingår i: Neurosurgery. - 0148-396X .- 1524-4040. ; 19:5, s. 767-771
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Tidskriftsartikel (refereegranskat)abstract
- Immunocomplexes (IC) in serum were analyzed in 54 patients with subarachnoid hemorrhage (SAH) from ruptured arterial aneurysms. A previous study had shown that patients with SAH and vasospasm had a significantly higher incidence of ICs in the blood than patients without vasospasm. The aim of the present study was to study how the IC content varied with time and compare this pattern with the clinical picture. Forty-two patients presented clinical or radiological signs of cerebral vasospasm during their hospital stays, whereas 12 patients showed no such signs. The patients with vasospasm had a significantly higher amount of ICs in serum than those without vasospasm. In 37 patients with vasospasm, the changes of IC content during the 1st weeks after SAH correlated well with the clinical course. Data indicated that a high IC content preceded the onset of vasospasm and a low content preceded clinical improvement. This observation supports the idea that the presence of ICs might be the cause and not the result of vasospasm.
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| 17. |
- Short, R. T., et al.
(författare)
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Production of very-low-energy highly charged ions by synchrotron radiation
- 1986
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Ingår i: Physical Review Letters. - 1079-7114. ; 56:24, s. 2614-2617
-
Tidskriftsartikel (refereegranskat)abstract
- Very-low-energy highly charged ions have been produced by use of white and monochromatic x rays from a wiggler line at the Stanford Synchrotron Radiation Laboratory to generate vacancy cascades following inner-shell photoionization. Recoil-ion energies have been determined and are shown to correspond essentially to room temperatures, even for high charge states. Promising applications to study of the interaction of stellar radiation with cold matter, to high-brightness ionsource development, to precision spectroscopy, and to angle-resolved chemical-physics reactive-scattering studies are discussed.
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| 18. |
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| 19. |
- Agardh, Carl-David, et al.
(författare)
-
Influence of treatment with diethylstilbestrol for carcinoma of prostate on platelet aggregation and plasma lipoproteins
- 1986
-
Ingår i: Urology. - : Elsevier BV. - 1527-9995 .- 0090-4295. ; 28:6, s. 469-471
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Tidskriftsartikel (refereegranskat)abstract
- Treatment of prostatic carcinoma with estrogens is accompanied by an increased risk for thromboembolic and cardiovascular complications. The underlying mechanisms are still unknown. Patients treated with diethylstilbestrol (DES) were compared with patients given no estrogen treatment regarding factors (platelet aggregation in vitro and plasma lipoproteins) that have been suggested to contribute to increased thrombogenesis and cardiovascular risk. The results do not show any increase in in vitro platelet aggregation in patients treated with DES compared with those given no treatment. This indicates that hyperaggregability does not contribute to the increased incidence in thromboembolic events seen in DES-treated patients. This is in contrast to the increased platelet aggregation previously described in patients treated with polyestradiolphosphate + etinylestradiol. The changes in plasma lipoproteins observed during DES-treatment are generally considered beneficial from an atherogenic point of view and do not appear to cause the elevated incidence of cardiovascular disease in these patients.
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| 20. |
- Agardh, Carl-David, et al.
(författare)
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The influence of treatment with estrogens and estramustine phosphate on platelet aggregation and plasma lipoproteins in non-disseminated prostatic carcinoma
- 1984
-
Ingår i: Journal of Urology. - 1527-3792. ; 132:5, s. 1021-1024
-
Tidskriftsartikel (refereegranskat)abstract
- The treatment of prostatic carcinoma with estrogens is associated with an increased risk of cardiovascular as well as thromboembolic complications. In the present study, patients harboring highly or moderately differentiated prostatic carcinoma without signs of metastases were treated with either polyestradiolphosphate + etinylestradiol, estramustine phosphate or given no treatment. Subsequently, these patients were investigated regarding factors (platelet aggregation, plasma and platelet phospholipid composition and lipoprotein patterns) that might contribute to increased thrombogenesis and cardiovascular risk. The results indicate the presence of increased in vitro platelet aggregation in patients treated with polyestradiolphosphate + etinylestradiol compared to those treated with estramustine phosphate or given no treatment. A possible relationship between the availability of arachidonic acid in platelet membrane phospholipids and in vitro platelet aggregation is suggested. On the other hand the alterations in plasma lipoproteins observed during treatment are generally considered positive from an atherogenic point of view and do not seem relevant to the elevated incidence of cardiovascular disease in these patients.
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| 21. |
- Bassant, M H, et al.
(författare)
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Electrophysiological and pharmacological properties of neurons within solid basal forebrain transplants in the rat brain
- 1988
-
Ingår i: Brain Research. - : Elsevier BV. - 0006-8993. ; 460:1, s. 8-16
-
Tidskriftsartikel (refereegranskat)abstract
- Electrophysiological and pharmacological properties of neurons within solid basal forebrain transplants were studied in adult rats anesthetized with urethane. No specific topography of the neurons recorded was observed within the graft. The mean spontaneous activity of the grafted neurons (GNs) was relatively low (4.9 impulses/s) but not unlike that of other central neurons in situ. A large proportion of GNs fired with regular discharges, but other modes of discharge were also observed. A few rhythmically bursting GNs were recorded having a discharge pattern very much like that of the rhythmically bursting medial septal neurons. The responses of GNs to glutamate, gamma-aminobutyric acid, acetylcholine, serotonin and norepinephrine was fairly similar to those described in other central structures.
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| 22. |
- Bau, R., et al.
(författare)
-
Effektiv utredning och rapportering
- 1989
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Föreliggande rapport innehåller råd, verktyg och dokumentationsstöd vid författande av akademiska rapporter och uppsatser inom främst ämnesområdena företagsekonomi och information management(informatik). Rapporten har använts under ett antal år inom grundutbildningen för blivande civilekonomer vid Handelshögskolan i Stockholm (HHS)
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| 23. |
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| 24. |
- Brundin, P, et al.
(författare)
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Behavioural effects of human fetal dopamine neurons grafted in a rat model of Parkinson's disease
- 1986
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Ingår i: Experimental Brain Research. - 0014-4819. ; 65:1, s. 40-235
-
Tidskriftsartikel (refereegranskat)abstract
- The ventral mesencephalon, containing the developing dopaminergic neurons of the substantia nigra-ventral tegmental region, was obtained from aborted human fetuses of 9-19 weeks of gestation. The tissue was grafted into the striatum of rats previously subjected to a 6-hydroxydopamine lesion of the mesostriatal dopamine pathway. The graft recipients were immunosuppressed by daily injections of Cyclosporin A. Amphetamine-induced motor asymmetry was reduced, and finally totally reversed, only in rats receiving grafts from the 9-week old fetal donor. The fluorescence microscopic analysis revealed large numbers of surviving dopamine neurons, and extensive fiber outgrowth into the host striatum, in these rats. By contrast, rats receiving grafts from 11-19 week old donors had at most only few surviving dopamine neurons. These results indicate that human fetal mesencephalic tissue may be an efficient source of dopamine neurons for functional intracerebral grafting in patients with Parkinson's disease.
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| 25. |
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| 26. |
- Brundin, P, et al.
(författare)
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Cyclosporin A increases survival of cross-species intrastriatal grafts of embryonic dopamine-containing neurons
- 1985
-
Ingår i: Experimental Brain Research. - 0014-4819. ; 60:1, s. 8-204
-
Tidskriftsartikel (refereegranskat)abstract
- The survival and function of cross-species (mouse-to-rat) grafts of fetal mesencephalic dopamine (DA) neurons, implanted as a cell suspension in the striatum of rats with lesions of the mesostriatal DA system, have been studied in animals with and without immunosuppression induced by Cyclosporin A (CyA). At 6 weeks after grafting 3 out of 7 non-CyA treated animals showed some degree of graft survival and variable functional compensation. In those three animals an average of 92 DA neurons per graft was counted. In the grafted animals treated with daily CyA injections, all grafts survived and produced partial or complete functional compensation, and they had an average of 557 DA neurons per graft. It is concluded that intracerebral graft survival and function can be greatly improved by CyA treatment and that the immunological protection of neural transplants in the brain is only partial.
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| 27. |
- Brundin, Patrik, et al.
(författare)
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Human fetal dopamine neurons grafted in a rat model of Parkinson's disease : immunological aspects, spontaneous and drug-induced behaviour, and dopamine release
- 1988
-
Ingår i: Experimental Brain Research. - 0014-4819. ; 70:1, s. 192-208
-
Tidskriftsartikel (refereegranskat)abstract
- We have used a rat model of Parkinson's disease (PD) to address issues of importance for a future clinical application of dopamine (DA) neuron grafting in patients with PD. Human mesencephalic DA neurons, obtained from 6.5-8 week old fetuses, were found to survive intracerebral cell suspension xenografting to the striatum of rats immunosuppressed with Cyclosporin A. The grafts produced an extensive new DA-containing terminal network in the previously denervated caudate-putamen, and they normalized amphetamine-induced, apomorphine-induced and spontaneous motor asymmetry in rats with unilateral lesions of the mesostriatal DA pathway. Grafts from an 11.5-week old donor exhibited a lower survival rate and smaller functional effects. As assessed with the intracerebral dialysis technique the grafted DA neurons were found to restore spontaneous DA release in the reinnervated host striatum to normal levels. The neurons responded with large increases in extracellular striatal DA levels after the intrastriatal administration of the DA-releasing agent d-amphetamine and the DA-reuptake blocker nomifensine, although not to the same extent as seen in striata with an intact mesostriatal DA system. DA fiber outgrowth from the grafts was dependent on the localization of the graft tissue. Thus, grafts located within the striatum gave rise to an extensive axonal network throughout the whole host striatum, whereas grafted DA neurons localized in the neocortex had their outgrowing fibers confined within the grafts themselves. In contrast to the good graft survival and behavioural effects obtained in immunosuppressed rats, there was no survival, or behavioural effects, of human DA neurons implanted in rats that did not receive immunosuppression. In addition, we found that all the graft recipients were immunized, having formed antibodies against antigens present on human T-cells. This supports the notion that the human neurons grafted to the non-immunosuppressed rats underwent immunological rejection. Based on an estimation of the survival rate and extent of fiber outgrowth from the grafted human fetal DA neurons, we suggest that DA neurons that can be obtained from one fetus may be sufficient to restore significant DA neurotransmission unilaterally, in one putamen, in an immunosuppressed PD patient.
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| 28. |
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| 29. |
- Brundin, P, et al.
(författare)
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Intracerebral xenografts of dopamine neurons : the role of immunosuppression and the blood-brain barrier
- 1989
-
Ingår i: Experimental Brain Research. - 0014-4819. ; 75:1, s. 195-207
-
Tidskriftsartikel (refereegranskat)abstract
- Fetal mesencephalic mouse tissue, rich in dopamine neurons, was xenografted as a dissociated cell suspension into the striatum of rats with unilateral 6-hydroxydopamine induced lesions of the mesostriatal pathway. The rats were either assigned to a 10-day, 21-day or 42-day Cyclosporin A (CyA) immunosuppression scheme, or given no immunosuppression. The functional effects of the grafts were followed over 6 months by monitoring changes in the recipient rats' amphetamine-induced turning behaviour. Without immunosuppression no grafts were functional at the end of the experiment. In the 10-, 21- and 42-day CyA treatment groups there was a significant reduction of rotational asymmetry at some timepoint following grafting in 26 of the 33 rats. However, by 6 months only 8 grafts remained functional suggesting that in several rats an immunological rejection took place following the termination of immunosuppression. This was supported by catecholamine histofluorescence analysis which revealed evidence of surviving grafts only in the few rats which had shown sustained functional graft effects at 6 months after grafting. In animals in which the grafts had undergone rejection, there was scar-like tissue in the striatum which appeared more extensive in rats that had lost their grafts after several weeks compared to rats in which the grafts were rejected at an early time-point. In a subgroup of the grafted animals the humoral antibody response against major transplantation antigens present on the grafted cells was investigated. All the studied rats were found to be immunized against the grafted mouse tissue following the intrastriatal implantation. This occurred irrespective of prior immunosuppressive treatment. In a parallel group of rats, the leakage of the blood-brain barrier was studied following intrastriatal implantation of a syngeneic fetal neural cell suspension. Evans Blue was infused into rats 3-12 days following transplantation surgery. At the early time-points there was a marked barrier leakage at the implantation site. This subsided with time such that there was minor leakage after 7-8 days and no leakage after 12 days. In summary, the results indicate the CyA is effective in promoting survival of intracerebral xenografts of fetal neural tissue, but that cessation of immunosuppressive treatment in most cases results in rejection of the grafted tissue. Temporary CyA treatment, even exceeding the time it takes for the blood-brain barrier to reform after transplantation surgery, is thus not sufficient to reliably support long term survival of xenografted dopamine neurons.
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| 30. |
- Chen, Q, et al.
(författare)
-
Hydrolysis of triacylglycerol arachidonic and linoleic acid ester bonds by human pancreatic lipase and carboxyl ester lipase
- 1989
-
Ingår i: Biochimica et Biophysica Acta. - : Elsevier BV. - 0006-3002. ; 1004:3, s. 85-372
-
Tidskriftsartikel (refereegranskat)abstract
- The hydrolysis of polyenoic fatty acid ester bonds with pure human colipase-dependent lipase, with carboxyl ester lipase (CEL) and with these enzymes in combination was studied, using [3H]arachidonic- and [14C]linoleic acid-labelled rat chylomicrons as a model substrate. During the hydrolysis with colipase-dependent lipase, the amount of 3H appearing in 1,2-X-diacylglycerol (DG) markedly exceeded that of 14C. When CEL was added in addition this [3H]DG was efficiently hydrolyzed. CEL alone hydrolyzed the triacylglycerol (TG) at a low rate. The hydrolysis pattern with human duodenal content was similar to that seen with colipase-dependent lipase and CEL in combination. Increasing the concentration of taurodeoxycholate (TDC) and taurocholate (TC) or of TDC alone stimulated the hydrolysis of [3H]- and [14C]TG, but increased the accumulation of labelled DG that could act as substrate for CEL. It is suggested that very-long-chain polyenoic fatty acids of DG formed during the action of the colipase-dependent lipase on TG containing these fatty acids may be a physiological substrate for CEL.
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| 31. |
- Clarke, D J, et al.
(författare)
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Synaptogenesis of grafted cholinergic neurons
- 1987
-
Ingår i: Annals of the New York Academy of Sciences. - : Wiley. - 0077-8923 .- 1749-6632. ; 495:1, s. 268-282
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Tidskriftsartikel (refereegranskat)
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| 32. |
- de Luca, S., et al.
(författare)
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Proteoglycans from chick limb bud chondrocyte cultures. Keratan sulfate and oligosaccharides which contain mannose and sialic acid
- 1980
-
Ingår i: Journal of Biological Chemistry. - 0021-9258. ; 255:13, s. 6077-6083
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Tidskriftsartikel (refereegranskat)abstract
- The precursors, [ 35S]sulfate and [2- 3H]mannose, were used to study the biosynthesis of keratan sulfate and other oligosaccharides on proteoglycans isolated from Day 8 cultures of chick limb bud chondrocytes. After alkaline borohydride treatment, three fractions with sialic acid were separated by molecular sieve chromatography. The first contained keratan sulfate which was purified by digestion with chondroitinase to remove chondroitin sulfate, followed by molecular sieve and ion exchange chromatography. The purified keratan sulfate contained about 8% of the 35S activity originally in monomer. The chains had an average length of about 40 monosaccharides and contained only trace amounts of mannose (less than 1 residue/three to four chains). The second fraction contained the majority of the [ 3H]mannose originally in monomer, but no 35S activity. This fraction appears to contain oligosaccharide-peptides of the asparagine-N-glycosylamine type because there were no reduced sugars present and the alkaline borohydride treatment extensively degraded the core protein. The composition of the oligosaccharides, with high proportions of mannose, N-acetylglucosamine, galactose, and sialic acid, was consistent with this suggestion. The third fraction consisted of a series of oligosaccharides with sizes between three to six saccharides. They contained N-acetylgalactosaminitol, indicating that they were attached to the core protein by O-glycoside bonds between N-acetylgalactosamine and hydroxyl groups on serine and threonine. Thus, proteoglycans contain two classes of oligosaccharides, a mannose-rich class characteristic of glycoproteins and an O-glycoside class characteristic of mucins, in addition to the chondroitin sulfate and keratan sulfate chains.
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| 33. |
- Glemberg, R., et al.
(författare)
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A general constitutive model for concrete structures
- 1986
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Ingår i: Computational modelling of reinforced concrete structures. - Swansea : Pineridge Press. - 0906674468 ; , s. 84-100
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 34. |
- Goldkuhl, G., et al.
(författare)
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Att specificera informationssystem : En användarorienterad och systematisk metodik
- 1982
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Bok (refereegranskat)abstract
- Informationssystem utvecklas för att stödja människor i deras verksamheter. Informationssystem används som stöd för människors kunskapsbildning och handlande i verksamheter. I den här boken presenteras en metodik för att specificera inforamtionssystem på ett användarorienterat och systematiskt sätt. Processen att specificera inforamtionssystem delas upp i tre steg:1. Förändringsanalys (Är det informationssystem som är en möjlig och lämplig lösning på en organisations problem?)2. Verksamhetsstudier (Vilka olika inforamtionssystem skall man utveckla och vilka bidrag skall de ge till verksamheten?)3. Informationsanalys (Vad skall informationssystemen göra, dvs vilka typer av meddelanden och regler skall de innehålla?)Metodiken presenteras med hjälp av illustrativt genomgående praktikfall. Förutom de tre ovan nämnda områdena behandlas även datasystemutformning, dvs hur användarnas specifikationer kan utnyttjas för att utforma önskade datasystem.Boken vänder sig till studerande vid universitet/högskolor och andra läroanstalter. Boken vänder sig även till personer verksamma i praktikfältet, såsom systemerare och informationsystemanvändare.Boken var förpublicerad som en rapport TRITA-IB-ADB-1091, Institutionen för Administrativ Databehandling (ADB), Kungliga Tekniska Högskolan (KTH) och Stockholms universiet (1979-08-01)
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| 35. |
- Graham, J. B., et al.
(författare)
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The Malmo polymorphism of coagulation factor IX, an immunologic polymorphism due to dimorphism of residue 148 that is in linkage disequilibrium with two other F.IX polymorphisms
- 1988
-
Ingår i: American Journal of Human Genetics. - 0002-9297. ; 42:4, s. 573-580
-
Tidskriftsartikel (refereegranskat)abstract
- A mouse monoclonal antibody (MAB 9,9) to coagulation factor IX (F.IX) detects a polymorphism in the plasma of normal people. Its epitope has been narrowed down to <6 amino acids in the activation peptide of the X-linked F.IX protein. The activation peptide contains a dimorphism - Thr:Ala - at position 148 of the protein. Using synthetic oligonucleotides, we have demonstrated that (1) the F.IX which reacts with 9.9 has Thr at position 148 and (2) that which does not has Ala. Positive reactors (148(thr)) are designated Malmo A, and negative reactors (148(ala)) are designated Malmo B. The plasma levels of AA women are indistinguishable from those of A men, and both B men and BB women are null against MAB 9.9. The plasma level of Malmo A in AB women is approximately half that of AA women, and 'lyonization' is clearly operating in the heterozygotes. The dimorphism is in strong linkage disequilibrium with two other intragenic RFLPs, TaqI and XmnI. Furthermore, intragenic crossing-over - including double crossing-over - appears to have occurred between the three sites. Seven of the eight possible haplotypes have been identified, five in men and two others in women. The immunoassay that identifies ~50% of the AB women in the pool of Malmo A females with 95% confidence identifies men unambiguously as A or B. The assay would be very useful for population-genetic studies of the Malmo epitope if the studies were limited to men.
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| 36. |
- Grubb, Anders, et al.
(författare)
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Immunohistochemical characterization of the amyloid deposits and quantitation of pertinent cerebrospinal fluid proteins in hereditary cerebral hemorrhage with amyloidosis
- 1987
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Ingår i: Stroke. - : Ovid Technologies (Wolters Kluwer Health). - 0039-2499 .- 1524-4628. ; 18:2, s. 431-440
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Tidskriftsartikel (refereegranskat)abstract
- Cystatin C, a protein inhibitor of lysosomal cysteine proteinases, was demonstrated by immunohistochemical techniques to be present in the birefringent amyloid deposits of the small arteries in the cerebrum, cerebellum, and leptomeninges of 10 Icelandic individuals with hereditary cerebral hemorrhage with amyloidosis. Specimens from other organs were investigated in one of the patients, and amyloid angiopathy characterized by an immunoreactivity of cystatin C was found in a submandibular lymph node. No immunoreactivity of amyloid fibril protein AA, kappa or lambda immunoglobulin light chain, or prealbumin was observed. Significantly low cerebrospinal fluid concentrations of cystatin C were found in all 9 investigated individuals with hereditary cerebral hemorrhage with amyloidosis. The concentrations of beta 2-microglobulin, albumin, and IgG in the cerebrospinal fluid were within normal limits. Isoelectric focusing showed that cystatin C from the cerebrospinal fluid of 9 patients with hereditary cerebral hemorrhage with amyloidosis had an isoelectric point identical to that of normal individuals. This investigation demonstrates that hereditary cerebral hemorrhage with amyloidosis may be diagnosed by two laboratory methods: immunohistochemical investigation of cystatin C in brain tissue specimens and quantitation of cystatin C in cerebrospinal fluid.
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| 37. |
- Guigas, R., et al.
(författare)
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Strong interaction effects in antiprotonic 6Li/7Li atoms
- 1984
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Ingår i: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 137:56, s. 323-328
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Tidskriftsartikel (refereegranskat)abstract
- The effects of strong interaction on the 3 –> 2 X-ray transition in antiprotonic 6Li/7Li atoms have been measured at the low energy -beam at CERN. For the shifts and widths of the levels the values [epsilon]2p = (-230 ï¿œ 72) eV, [Gamma]2p = (443 ï¿œ 210) eV, and [Gamma]3d = (0.130 ï¿œ 0.045) eV for 6Li, and [epsilon]2p = (-336 ï¿œ 60) eV, [Gamma]2p = (456 ï¿œ 190) eV, and [Gamma]3d = (0.210 ï¿œ 0.062) eV for 7Li were found. The data are compared with optical model predictions.
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| 38. |
- Harris, A.S., et al.
(författare)
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Effect of viscosity on the pharmacokinetics and biological response to intranasal desmopressin.
- 1989
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Ingår i: Journal of Pharmaceutical Sciences. - : Elsevier BV. - 0022-3549. ; 78:6, s. 470-471
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Tidskriftsartikel (refereegranskat)abstract
- he effect of viscosity on the nasal absorption and biological response to desmopressin was studied in humans. Nasal solutions of desmopressin with and without the addition of 0.25% (w/v) methylcellulose were administered by a precompression nasal spray pump to 10 volunteers. Plasma levels of desmopressin were assayed by radioimmunoassay and the biological response was measured by determination of the antihemophilia factors (Factor VIII and von Willebrand factor). The results showed that the addition of methylcellulose produced a more sustained and slower absorption, with a longer time to maximum plasma concentration (tmax). However, the areas under the plasma concentration—time curve were not different, indicating a similar total bioavailability. The biological response showed a similar effect. Peak Factor VIII activity was not different, but the presence of methylcellulose produced a slower onset of activity. These findings indicate that although the addition of a viscous agent to nasal formulations may produce a more sustained effect, it delays the onset of activity and no enhancement is achieved in the total bioavailability.
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| 39. |
- Holmberg, Lars, et al.
(författare)
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Prenatal diagnosis of hemophilia B by an immunoradiometric assay of factor IX
- 1980
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Ingår i: Blood. - 0006-4971. ; 56:3, s. 397-401
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Tidskriftsartikel (refereegranskat)abstract
- An immunoradiometric assay of factor IX was developed based on homologous antibodies that arose in a hemophilic patient. With this assay, 11 of 12 patients with severe hemophilia B had factor IX antigen levels below 1 U/dl and 6 patients with mild hemophilia B had various levels. Factor IX antigen in 8 fetuses (16th-20th gestational week) aborted for therapeutic reasons ranged from 1.8 to 10.0 U/dl. Six amniotic fluids contained 0.28-1.2 U/dl factor IX antigen. Using the immunoradiometric assay, we could diagnose hemophilia B prenatally in one fetus at risk. No factor IX antigen (< 0.2 U/dl) was detectable in the fetoscopic sample. After termination of the pregnancy, analysis of blood from the abortus confirmed the diagnosis of severe hemophilia B. We conclude that very sensitive immunologic assays, such as the one described here, will prove useful in prenatal diagnosis of severe hemophilia B, since determination of factor IX activity in fetoscopic samples is unrealiable because of possible contamination with thromboplastic material.
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| 40. |
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| 41. |
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| 42. |
- Kjellén, Elisabeth, et al.
(författare)
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Effect of hyperthermia and/or nicotinamide on the radiation response of a C3H mammary carcinoma
- 1989
-
Ingår i: European journal of cancer & clinical oncology. - : Elsevier BV. - 0277-5379. ; 25:12, s. 1733-1737
-
Tidskriftsartikel (refereegranskat)abstract
- The effect of hyperthermia and/or nicotinamide (200 mg/kg of body weight) on the tumour growth delay induced by radiation was evaluated in a C3H mouse mammary adenocarcinoma. The study showed a radiosensitizing effect of hyperthermia and of nicotinamide but the combination of all three modalitites showed no increased tumor growth delay compared to hyperthermia and radiation alone. The tumour growth delay induced by hyperthermia was not modified by nicotinamide.
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| 43. |
- Landin, Britta, et al.
(författare)
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A role for hepatic lipase in chylomicron and high density lipoprotein phospholipid metabolism
- 1984
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Ingår i: Journal of Lipid Research. - 0022-2275. ; 25:6, s. 63-559
-
Tidskriftsartikel (refereegranskat)abstract
- The rate of removal of phosphatidylethanolamine and phosphatidylcholine from the plasma of rats treated with antiserum to hepatic lipase was measured. The hepatic lipase antiserum was injected intravenously into animals prior to injection of 32P-labeled chylomicrons or 32P-labeled high density lipoproteins. In experiments in which 32P-labeled chylomicrons were injected, antiserum treatment inhibited removal of [32P]phosphatidylethanolamine from chylomicrons, and the unlabeled serum phosphatidylethanolamine levels increased 2-2.5-fold in 30 min. In contrast, hepatic lipase antiserum had no significant effect on the clearance of chylomicron [32P]phosphatidylcholine or on unlabeled phosphatidylcholine concentrations in serum after injection of chylomicrons. In experiments in which 32P-labeled high density lipoproteins were injected, the inhibitory effect of the antiserum on the rapid removal of [32P]phosphatidylethanolamine from the circulation was even more marked than its effect on the removal from chylomicrons. The removal of high density lipoprotein phosphatidylcholine on the other hand was unaffected by the antiserum, although a moderate increase in serum phosphatidylcholine concentration was seen. In antiserum-treated rats injected with 32P-labeled chylomicrons or high density lipoproteins, hepatic [32P]phosphatidylethanolamine radioactivity was decreased. Significantly more [32P]phosphatidylethanolamine was recovered from blood plus liver in the antiserum-treated rats, indicating that the antiserum inhibited the overall degradation of injected [32P]phosphatidylethanolamine. The data suggest that phosphatidylethanolamine is a preferred substrate for hepatic lipase in the metabolism of chylomicron and high density lipoprotein phospholipid.
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| 44. |
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| 45. |
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| 46. |
- Ljung, R., et al.
(författare)
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Two allotypes of factor IX present in haemophilia B
- 1986
-
Ingår i: Scandinavian Journal of Haematology. - : Wiley. - 0036-553X. ; 37:5, s. 411-416
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Tidskriftsartikel (refereegranskat)abstract
- Factor IX antigen (IX:Ag) was measured with three different immunoradiometric assays (IRMAs) in 30 healthy people and 43 patients with haemophilia B of varying severity. Two of the IRMAs were based on monoclonal antibodies capable of differentiating between two genetically determined molecular variants of normal factor IX. Most patients with severe hemophilia B lacked demonstrable IX:Ag. The factor IX variant that is undetectable with one of the monoclonal antibodies used was present in 2 out of 6 families with moderate haemophilia B and in 1 out of 6 families with mild haemophilia B. The existence of allotypes of factor IX in hemophilia B may have practical implications for carrier detection and prenatal diagnosis.
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| 47. |
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| 48. |
- Lundberg, Bengt, et al.
(författare)
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Nonlinear dissipative spring mass model for a percussive drill rod joint of the coupling sleeve type
- 1989
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Ingår i: International Journal of Impact Engineering. - 0734-743X .- 1879-3509. ; 8:4, s. 303-313
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Tidskriftsartikel (refereegranskat)abstract
- A nonlinear dissipative spring mass (NDSM) model for a percussive drill rod joint of the coupling sleeve (CS) type is established. Such a joint consists of a cylindrical coupling sleeve with internal thread which connects two drill rods with external threads at their ends. The model disregards wave motion in the coupling sleeve but accounts for axial mobility of the sleeve relative to the rods. This mobility is due to local deformation and slip of the threads. The model is characterized by the mass of the sleeve and by three parameters which represent the coupling between the sleeve and the drill rods through the threads. A static and a dynamic test have been developed for determining the coupling parameters. The model is validated by simulating previous impact tests with a commercial CS joint.
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| 49. |
- Mulvany, M J, et al.
(författare)
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Potentiating and depressive effects of ouabain and potassium-free solutions on rat mesenteric resistance vessels.
- 1982
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Ingår i: Circulation research. - 0009-7330. ; 51:4, s. 514-24
-
Tidskriftsartikel (refereegranskat)abstract
- We have investigated the in vitro effects of ouabain and K-free solutions on some pharmacological and electrophysiological properties of rat mesenteric resistance vessels (internal diameter approximately 190 micrometers). Vessels were mounted as ring preparations on a myograph capable of measuring their isometric wall tension. In normal saline solutions, vessels did not exhibit any tone and had a membrane potential of -54 mV. Both 1 mM ouabain and K-free solutions caused a transient depolarization of 5-8 mV; thereafter the membrane slowly depolarized to about -45 mV after 30 minutes. There was no mechanical response to ouabain, but K-free solutions caused a transient development of tension which could be inhibited by phentolamine (1 microM). In norepinephrine-activated vessels, exposure to ouabain or K-free solutions caused a small depolarization and an increase in tension. Long-term (30-minute) exposure to 1 mM ouabain or K-free solutions reduced the amplitude of norepinephrine responses and, for the lower (but not the higher) norepinephrine concentrations, the membranes were about 14 mV more depolarized than control. The mechanical responses to a cocktail of norepinephrine in a high potassium solution were, however, unaffected. Re-exposure to normal saline solution produced a transient hyperpolarization and transiently eliminated the norepinephrine response, but thereafter the membrane potential and response returned to normal. The results indicate that ouabain and K-free solutions can have both short-term potentiating and long-term depressive effects on the mechanical response of rat mesenteric resistance vessels to norepinephrine.
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| 50. |
- Mulvany, M J, et al.
(författare)
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Role of membrane potential in the response of rat small mesenteric arteries to exogenous noradrenaline stimulation.
- 1982
-
Ingår i: The Journal of physiology. - 0022-3751. ; 332, s. 363-73
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Tidskriftsartikel (refereegranskat)abstract
- 1. We have made simultaneous measurements of membrane potential and wall tension in rat 200 microns mesenteric arteries. 2. The resting membrane potential was -59.2 +/- 0.4 mV and stable (218 measurements, fifty-two vessels). 3. With maximal exogenous noradrenaline stimulation (10 microM) the membrane depolarized to about -34 mV. During the onset of tension development oscillations (period about 6 sec) in both tension and membrane potential were often seen; the membrane potential changes led the tension changes by about 1.2 sec. 4. In the presence of increased K+ (e.g. 40 mM), vessels had an increased noradrenaline sensitivity, and here noradrenaline stimulation produced little change in membrane potential. 5. With maximal K+ stimulation (85 mM), in the presence of phentolamine (1 microM), the membrane depolarized to about -17 mV, the tension being about 70% of the maximal noradrenaline response. 6. In the presence of phentolamine (1 microM), noradrenaline caused hyperpolarization without tension development. The hyperpolarization was inhibited by propranolol and mimicked by isoprenaline. 7. The results suggest that in these small vessels membrane potential variations are not essential to, but have an important modulating influence on, the tension response to exogenous noradrenaline.
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