SwePub
Sök i SwePub databas

  Utökad sökning

Träfflista för sökning "WFRF:(Nicholls Ian professor) srt2:(2009)"

Sökning: WFRF:(Nicholls Ian professor) > (2009)

  • Resultat 1-2 av 2
Sortera/gruppera träfflistan
   
NumreringReferensOmslagsbildHitta
1.
  • Åslund, Andreas, 1978- (författare)
  • Designing thiophene-based fluorescent probes for the study of neurodegenerative protein aggregation diseases : From test tube to in vivo experiments
  • 2009
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Protein aggregation is an event related to numerous neurodegenerative diseases, such as Alzhemier’s disease and prion diseases. However little is known as to how and why the aggregates form and furthermore, the toxic specie may not be the mature fibril but an on route or off route specie towards mature aggregates. During this project molecular probes were synthesized that may shed some light to these questions. The probes are thiophene based and the technique used for detection was mainly fluorescence. It was shown that the previously established thiophene based in vitro staining technique is valid ex vivo and in vivo. This would not have been possible without the synthesis of a variety of functionalized polymeric thiophene based probes; their in vitro and ex vivo staining properties were taken into consideration when the design of the small oligomeric probes were decided upon. These probes were shown to spectrally distinguish different types of amyloid, pass the bloodbrain barrier within minutes and specifically and selectively stain protein aggregates in the brains of mice.
  •  
2.
  • Dunér, Gunnar, 1980- (författare)
  • Signal Enhancement by Dynamic Polymers in Quartz Crystal Microbalance Applications
  • 2009
  • Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
    •  This licentiate thesis presents studies of signal enhancement by dynamic polymers in quartz crystal microbalance (QCM) applications. The aim of the study has been to determine the potential for the use of polymers on QCM sensors for signal enhancement in molecular interaction studies. A method for synthesis of polymer sensor surfaces on QCM substrates was developed based on an iniferter photo polymerization technique. Polymerized poly(acrylic acid) and poly(acryl amide) surfaces were extensively characterized with electron spectroscopy for chemical analysis (ESCA), infrared reflection absorption spectroscopy (IRAS) and atomic force microscopy (AFM). The QCM response dynamics of these surfaces to pH changes was studied and the carboxyl containing surfaces exhibited large, reversible and highly reproducible frequency shifts due to expansion of deprotonated polymer chains. Surface acid dissociation constants (pKa) were determined for poly(acrylic acid) surfaces, carboxymethyldextran surfaces and self-assembled monolayer carboxyl surfaces by means of pH titration in QCM flow-through instrumentation. Surface pKa data was consistent with available literature data. The sensitivity enhancement for molecular interaction studies was investigated for poly(acrylic acid) coated QCM sensors with a model system consisting of immobilized biotin and an anti-biotin Fab fragment. Binding responses of antibiotin Fab to immobilized biotin was revealed to be 11 times higher than the corresponding results for a carboxylated SAM surface.  
  •  
Skapa referenser, mejla, bekava och länka
  • Resultat 1-2 av 2

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy