| 1. |
- Ager, S, et al.
(författare)
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Retroviral display of antibody fragments; interdomain spacing strongly influences vector infectivity
- 1996
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Ingår i: Human Gene Therapy. - : Mary Ann Liebert Inc. - 1043-0342 .- 1557-7422. ; 7:17, s. 2157-2164
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Tidskriftsartikel (refereegranskat)abstract
- Five different single-chain antibody fragments (scFv) against human cell-surface antigens were displayed on murine ecotropic retroviral vectors by fusing them to the Moloney SU envelope glycoprotein. The spacing between the scFv and the SU glycoprotein was varied by fusing the scFv to residue +7 or to residue +1 of Moloney SU and by inserting linker sequences of different lengths between the domains. All of the chimeric envelopes were efficiently incorporated into vector particles and could bind to human cells through their displayed antibody fragments, but did not infect them. The spacing between the scFvs and the SU glycoproteins had no significant effect on the efficiency of envelope expression or viral incorporation and did not affect the binding properties of the chimeric envelopes, nor did it influence the efficiency of targeted gene delivery to human cells by scFv-displaying vectors. However, on murine fibroblasts the infectivity of vectors incorporating the chimeric envelopes was strongly influenced by the length of the interdomain spacer. The titers were very low when the single-chain antibodies were fused through a tripeptide linker to SU residue +7 and were greatly enhanced (up to 10(5)-fold) when they were fused to SU residue +1 through a heptapeptide linker. These results point to the importance of steric interactions between the domains of chimeric envelope glycoproteins and may have implications for retroviral vector design for human gene therapy.
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| 2. |
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| 3. |
- Bergman, B, et al.
(författare)
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An intraindividual clinical comparison of 2 metal-ceramic systems.
- 1999
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Ingår i: International Journal of Prosthodontics. - 0893-2174 .- 1139-9791. ; 12:5, s. 444-7
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Tidskriftsartikel (refereegranskat)abstract
- PURPOSE: It has been questioned whether the surface and color of the ceramic and the metal-ceramic bond strength of a titanium-ceramic system are comparable to those of a conventional noble alloy-ceramic system. It was therefore the aim of this study to carry out an intraindividual clinical comparison between crowns fabricated according to the Procera system (titanium copings veneered with a low-fusing ceramic) and noble-alloy copings veneered with a medium-fusing ceramic. MATERIALS AND METHODS: Twenty-one crown pairs were fabricated for eighteen patients; three of the patients were each provided with two crown pairs. After 2 years nineteen crown pairs in sixteen patients could be compared. Clinical examinations were performed by two calibrated dentists who are long experienced in prosthetic dentistry. The crowns were rated according to the California Dental Association system. In addition, Bleeding Index and Margin Index were evaluated. RESULTS: After 2 years the quality of surface and color of the ceramic material seemed to have deteriorated more in titanium-ceramic crowns than in conventional metal-ceramic crowns, although the difference was not statistically significant. Regarding anatomic form, margin integrity, Bleeding Index, and Margin Index the differences between the two crown systems were small. CONCLUSION: The low-fusing ceramics have been subject to improvements during the last few years. Their bond strength to titanium seems to be comparable to that of conventional metal-ceramic systems. However, in the long run one problem may be the surface and color stability of low-fusing ceramics. To make extended long-term comparisons between the two metal-ceramic systems possible the present patient material will be followed for a longer period than the current 2 years.
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| 4. |
- Friberg, Bertil, 1950, et al.
(författare)
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Mk II: the self-tapping Brånemark implant: 5-year results of a prospective 3-center study.
- 1997
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Ingår i: Clinical oral implants research. - 0905-7161. ; 8:4, s. 279-85
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Tidskriftsartikel (refereegranskat)abstract
- The 5-year result of a prospective 3-center study is presented, comprising 103 patients with 288 Mk II self-tapping and 275 standard implants of the Brånemark System. Out of 363 mandibular and 200 maxillary fixtures, one Mk II was lost of the lower jaw and 13 of each implant type failed in maxillae during the study period. Cumulative prosthesis stability was 97%. Five patients accounted for more than 85% of the fixture losses. Marginal bone resorption was similar for both implant designs. Apart from the implant failures and one patient exhibiting disturbed nerve sensation of the mental nerve, no major complications were encountered. Overall, this study revealed equal cumulative success rates for standard and Mk II implants after 5 years of observation. Mandibular implants exhibited greater success rates (100%) for both tested implant types compared to maxillary implants (87%).
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| 5. |
- Olsson, M, et al.
(författare)
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MkII--a modified self-tapping Brånemark implant: 3-year results of a controlled prospective pilot study.
- 1995
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Ingår i: The International journal of oral & maxillofacial implants. - 0882-2786. ; 10:1, s. 15-21
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Tidskriftsartikel (refereegranskat)abstract
- A modified self-tapping implant (MkII) with improved cutting characteristics has been designed for use in both maxillae and mandibles. Four sequential studies were conducted to evaluate the outcome of the MkII implant compared to the standard implant. The results presented here are from the extended pilot study that has been conducted as an intra-individual study of 103 patients; ie, each patient received both test (MkII) and control (standard Brånemark System) implants. Seventy patients were treated in the mandible and 33 in the maxilla. The cumulative survival rates and marginal bone resorption showed equivalent results for both test and control implants. Three-year cumulative survival rates were 87.9% and 86.8% for test and control implants in maxilla, respectively, and 99.5% and 100% in mandibles, respectively. The mean marginal bone resorption was approximately 0.5 (control) to (MK II) 0.6 mm after 3 years of function. A total of 288 test implants and 275 control implants were placed. All implants, both test and control, could be placed in an appropriate implant position, but 21.2% of the implants were not fully seated by machine power only; the use of a manual cylinder wrench for the final turns was necessary. During the last phase of the study, however, with an increase in twist-drill diameter from 3.0 to 3.15 mm and an increased motor torque, the prerequisites of successful implant placement into final position were met.
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