| 1. |
- Eeg-Olofsson, Orvar, et al.
(författare)
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Duchenne muscular dystrophy and idiopathic hyperCKemia in the same family
- 2008
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Ingår i: European journal of paediatric neurology. - : Elsevier BV. - 1090-3798 .- 1532-2130. ; 12:5, s. 404-7
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Tidskriftsartikel (refereegranskat)abstract
- Familial hyperCKemia is a rare condition, and a combination with Duchenne muscular dystrophy (DMD) is extremely rare. A boy showed muscle weakness from the age of 10 months and presented typical signs of DMD at the age of 18 months. The diagnosis was supported by markedly elevated serum creatine kinase (CK) value as well as by neurophysiological and muscle biopsy findings at the age of 23 months. The diagnosis was confirmed by identification of a stop codon in exon 43 (p.2095Arg>X) of the dystrophin gene. Interestingly, the father and his near relatives had increased serum CK values without any clinical symptoms or signs, nor a defect in caveolin-3 gene. We suggest that the occurrence of familial hyperCKemia may have triggered the early onset of symptoms in our patient.
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| 2. |
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| 3. |
- Edebol Eeg-Olofsson, Karin
(författare)
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Sphincter dysfunction
- 2005
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Ingår i: Clinical Neurophysiology of infancy, childhood and adolescence. - : Elsevier, USA. ; , s. 827-831
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Bokkapitel (populärvet., debatt m.m.)
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| 4. |
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| 5. |
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| 6. |
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| 7. |
- Hult, Agneta, et al.
(författare)
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Tio år senare - Lärares syn på yrket i en skola i förändring
- 2005
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Ingår i: Lärarrollen i ett föränderligt samhälle. En antologi om några forskningsprojekt som fått stöd från Länsförsäkringsbolagens forskningsstöd. - Stockholm : Länsförsäkringar. ; , s. 43-72
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 8. |
- Hult, A, 2005, et al.
(författare)
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Tio år senare - Lärares syn på yrket i en skola under förändring
- 2005
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Ingår i: Länsförsäkringar. Lärarrollen i ett föränderligt samhälle : en antologi om några forskningsprojekt som fått stöd från Länsförsäkringsbolagens forskningsfond. Stockholm: Länsförsäkringsbolagens AB (LFAB). - Stockholm : Länsförsäkringsbolagen (LFAB). - 9789163184338 ; , s. 43-72
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 9. |
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| 10. |
- Kärrlander, Maria, et al.
(författare)
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Histidine-rich glycoprotein can prevent development of mouse experimental glioblastoma
- 2009
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Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 4:12, s. e8536-
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Tidskriftsartikel (refereegranskat)abstract
- Extensive angiogenesis, formation of new capillaries from pre-existing blood vessels, is an important feature of malignant glioma. Several antiangiogenic drugs targeting vascular endothelial growth factor (VEGF) or its receptors are currently in clinical trials as therapy for high-grade glioma and bevacizumab was recently approved by the FDA for treatment of recurrent glioblastoma. However, the modest efficacy of these drugs and emerging problems with anti-VEGF treatment resistance welcome the development of alternative antiangiogenic therapies. One potential candidate is histidine-rich glycoprotein (HRG), a plasma protein with antiangiogenic properties that can inhibit endothelial cell adhesion and migration. We have used the RCAS/TV-A mouse model for gliomas to investigate the effect of HRG on brain tumor development. Tumors were induced with platelet-derived growth factor-B (PDGF-B), in the presence or absence of HRG. We found that HRG had little effect on tumor incidence but could significantly inhibit the development of malignant glioma and completely prevent the occurrence of grade IV tumors (glioblastoma).
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| 11. |
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| 12. |
- Löf, David, et al.
(författare)
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A Calorimetry and Light Scattering Study of the Formation and Shape Transition of Mixed Micelles of EO20PO68EO20 Triblock Copolymer (P123) and Nonionic Surfactant (C12EO6)
- 2007
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Ingår i: The Journal of Physical Chemistry Part B. - : American Chemical Society (ACS). - 1520-5207 .- 1520-6106. ; 111:21, s. 5911-5920
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Tidskriftsartikel (refereegranskat)abstract
- The interaction between the nonionic surfactant C12EO6 and the poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer EO20PO68EO20 (P123) has been investigated by means of isothermal titration and differential scanning calorimetry (DSC) as well as static and dynamic light scattering (SLS and DLS). P123 self-assembles in water into spherical micelles at ambient temperatures. At raised temperatures, the DSC data revealed a sphere-to-rod transition of the P123 micelles around 60 degrees C. C12EO6 interacts strongly with P123 micelles in aqueous solution to give mixed micelles with a critical micelle concentration (cmc) well below the cmc for pure C12EO6. The presence of C12EO6 also lowers the critical micelle temperature of P123 so aggregation starts at significantly lower temperatures. A new phenomenon was observed in the P123-C12EO6 system, namely, a well-defined sphere-to-rod transition of the mixed micelles. A visual phase study of mixtures containing 1.00 wt % P123 showed that in a narrow concentration range of C12EO6 both the sphere-to-rod transition and the liquid-liquid phase separation temperature are strongly depressed compared to the pure P123-water system. The hydrodynamic radius of spherical mixed micelles at a C12EO6/P123 molar ratio of 2.2 was estimated from DLS to be 9.1 nm, whereas it is 24.1 nm for the rodlike micelles. Furthermore, the hydrodynamic length of the rods at a molar ratio of 2.2 is in the range of 100 nm. The retarded kinetics of the shape transition was detected in titration calorimetric experiments at 40 degrees C and further studied by using time-resolved DLS and SLS. The rate of growth, which was slow (> 2000 s), was found to increase with the total concentration.
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| 13. |
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| 14. |
- Michaëlsson, Karl, et al.
(författare)
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Leisure physical activity and the risk of fracture in men
- 2007
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Ingår i: PLoS Medicine. - : Public Library of Science (PLoS). - 1549-1277 .- 1549-1676. ; 4:6, s. 1094-1100
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Data from previous studies are inconsistent, and it is therefore uncertain whether, to what extent, and at what level leisure physical activity influences the risk of osteoporotic fractures in men. METHODS AND FINDINGS: A cohort of 2,205 men, 49-51 y of age, was enrolled in a longitudinal, population-based study. Leisure physical activity and other lifestyle habits were established at baseline and at ages 60, 70, 77, and 82 y. During 35 y of follow-up, 482 men had at least one fracture. Cox's proportional hazards regression was used to determine hazard ratios (HRs) of fracture associated with time-dependent physical activity habits and covariates. Men with a sedentary lifestyle (HR 2.56, 95% confidence interval 1.55-4.24) or men who walked or bicycled only for pleasure (HR 1.61, 95% confidence interval 1.10-2.36) had an increased adjusted risk of hip fracture compared with men who participated in regular sports activities for at least 3 h/wk. At the end of follow-up, 8.4% of the men with a high physical activity, 13.3% of the men with a medium physical activity, and 20.5% of the men with a low physical activity had suffered a hip fracture. According to the estimation of population-attributable risk, one third of all hip fractures could be prevented by participation in regular sports activities. High activity also conferred a reduced overall fracture risk. CONCLUSIONS: Our data indicate that regular sports activities can reduce the risk of fractures in older men.
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| 15. |
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| 16. |
- Olofsson, Birgitta, 1963-
(författare)
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Old people with femoral neck fracture : delirium, malnutrition and surgical methods - an intervention program
- 2007
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Hip fracture is a global and a growing public health problem. More women than men sustain hip fractures, the incidence increases exponentially with age and mean age is above 80. About one third of hip-fracture patients suffer from dementia and are prone to develop acute confusional state (delirium). Delirium is one of the most common complications after hip-fracture surgery, and seriously impacts on morbidity and mortality. Malnutrition is also common in hip-fracture patients and is associated with postoperative complications, such as delayed healing of the wound, infections and decubitus ulcers. Arthroplasty is usually preferred procedure in displaced femoral neck fractures but is, however, controversial in patients with dementia due to the fear of dislocation of the prosthesis. The aims of this thesis are to identify risk factors for delirium and the impact of delirium on rehabilitation outcome, to evaluate whether a postoperative multi-factorial intervention program could reduce delirium, to investigate the effect of a nutritional intervention and to evaluate complications, functional outcome and mortality regarding two surgical methods, hemiarthroplasty (HAP) and internal fixation (IF), in old patients with femoral neck fracture. Thirty-eight out of 61 consecutive patients (62%) were delirious on admission to hospital or developed postoperative delirium. An increased risk of postoperative delirium was found among hip-fracture patients with dementia and/or depression. Delirious patients were hospitalized longer, were more dependent in their activities of daily living, had poorer psychological well-being and suffered more complications than non-delirious patients. A postoperative multi-factorial and multidisciplinary intervention program reduced the incidence, at 55% vs 75% (p=0.003), and number of days with delirium, 5 vs 10 days (p=0.009). Postoperative complications were also reduced; decubitus ulcers 9% vs 22% (p= 0.010), urinary tract infections 31% vs 51% (p=0.005), falls 12% vs 27% (p=0.007), and the mean hospitalization period was 10 days shorter in the intervention group (p=0.030). Malnutrition was common among all these patients (53 %) and associated with postoperative complications such as decubitus ulcers and delirium. However, the nutritional intervention had no effect on nutritional parameters at four months, nevertheless men had better nutritional outcomes than women. A higher proportion of patients with dementia operated on using HAP had regained their pre-fracture ability to walk independently at the one-year follow up compared with those operated on using IF. Six of 83 patients dislocated their HAP during hospitalization and during an episode of delirium, none had dementia. No difference in mortality between the surgical methods was seen. Dementia per se should not be a reason to disqualify patients from being treated with the most appropriate surgical method. It is clinically important to discriminate between dementia and delirium, since delirium can be prevented and treated even in patients with dementia. Old patients undergoing surgery have special needs that are not always catered for in ordinary orthopaedic or surgical wards. The special care for these patients should include: a combined nursing and medical care based on comprehensive geriatric assessments, systematic prevention, detection and treatment of postoperative complications such as delirium, hypoxemia, urinary tract infections, pain, malnutrition and an active rehabilitation. It is obvious that improved quality of care reduces patient suffering and seemingly the costs for society.
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| 17. |
- Olofsson, Ingvar, 1943, et al.
(författare)
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Beständiga Konstruktioner
- 2007
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Ingår i: Vbyggaren. ; :4, s. 46-52
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Samhällets investeringar i infrastruktur och andra byggobjekt är betydande. Ett rimligt krav är därföratt de tekniska och ekonomiska förutsättningar som är kopplade till konstruktionernas beteende övertid skall vara kända redan i tidiga skeden. Projektering, upphandling och genomförande av byggprojektsker i dag med utgångspunkt från ett begränsat synsätt avseende konstruktionernas beteende i ettlivslängdsperspektiv. Ökad kunskap krävs i första hand beträffande sammansatta konstruktionersnedbrytningsförlopp. Fungerande och samordnade modeller för tidig teknisk och ekonomisk värderingav underhållsinsatser måste också utvecklas.
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| 18. |
- Santillo, Alexander, et al.
(författare)
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Frontotemporal Dementia-amyotrophic Lateral Sclerosis Complex is Simulated by Neurodegeneration With Brain Iron Accumulation
- 2009
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Ingår i: Alzheimer Disease and Associated Disorders. - 0893-0341 .- 1546-4156. ; 23:3, s. 298-300
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Tidskriftsartikel (refereegranskat)abstract
- We describe a case of late onset neurodegeneration with brain iron accumulation (NBIA) presenting as frontotemporal dementia (FTD) with amyotrophic lateral sclerosis (ALS). A male patient presented at age 66 with change of personality: disinhibition, emotional blunting, and socially inappropriate behavior, coupled with dysarthria, dystonia, and corticospinal tract involvement. Magnetic resonance imaging showed general cortical atrophy, iron deposits in the globus pallidus, and the "eye of the tiger" sign. Neuropsychologic performance was globally reduced, especially executive functions. Fluorodeoxyglucose positron emission tomography showed hypometabolism predominantly in frontal and temporal areas. Repeated neurophysiologic examinations showed signs of chronic denervation. The patient was diagnosed with NBIA but fulfilled consensus criteria for FTD and had a clinical picture of ALS, without neurophysiologic confirmation. Our finding introduces NBIA as a possible cause of FTD and as a differential diagnosis of the FTD-ALS complex.
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| 19. |
- Stuart, Andrea, et al.
(författare)
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Fetal electrocardiographic monitoring during labor in relation to cord blood levels of the brain-injury marker protein S-100.
- 2008
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Ingår i: Journal of Perinatal Medicine. - 1619-3997 .- 0300-5577. ; 36:2, s. 136-141
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Tidskriftsartikel (refereegranskat)abstract
- Abstract Background: Cord artery protein S-100 levels at birth are potential markers of brain damage after asphyxia. Our aim was to investigate if S-100 levels were elevated in neonates with indirect signs of asphyxia during birth. S-100 levels in cord blood were studied in relation to cardiotocography (CTG) and fetal electrocardiography (FECG) changes during birth and to acidemia in umbilical blood. Material and methods: This case-control study was performed in parallel to a large randomized controlled trial (RCT) studying FECG at birth. Protein S-100 samples were collected from 103 neonates at birth and related to the CTG and ECG changes during labor and to pH in umbilical blood. Results: Protein S-100 was significantly higher in neonates with umbilical artery blood pH7.05. Furthermore, neonates with preterminal CTG patterns showed increased S-100 levels compared to neonates with normal CTG. Neonates having significant CTG and ECG changes, leading to intervention according to clinical guidelines, showed significantly higher S-100 levels compared to neonates without such indication of intervention. Conclusion: A relation exists between S-100 in umbilical blood at birth, acidosis and pathological patterns in CTG and FECG during labor.
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| 20. |
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| 21. |
- Wiberg, Nana, et al.
(författare)
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Base deficit estimation in umbilical cord blood is influenced by gestational age, choice of fetal fluid compartment, and algorithm for calculation.
- 2006
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Ingår i: American Journal of Obstetrics and Gynecology. - : Elsevier BV. - 1097-6868 .- 0002-9378. ; 195:6, s. 1651-1656
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Tidskriftsartikel (refereegranskat)abstract
- Objective: The purpose of this study was to explore the influences of gestational age, the choice of fetal fluid compartment, and the algorithm for calculation on the estimation of the base deficit in umbilical cord arterial blood Lit birth. Study design: From 1995 to 2002, cord arterial blood gases and obstetric data were available for 43,551 newborn infants at 37 + weeks of gestation (cohort 1). The mean base deficit in blood and the base deficit in extracellular fluid were estimated from pH and P-CO2 values in 28,213 newborn infants with a 5-minute Apgar score of >= 9 (cohort 11) with the use of 3 different calculation algorithms (base deficit in blood, base deficit in extracellular fluid [A], and base deficit in extracellular fluid [B]). Results: In cohort 11, the base deficit in blood, the base deficit in extracellular fluid (A), and the base deficit in extracellular fluid (B) increased with advancing gestational age (linear regression, P < .0001). The curves run almost parallel, with the base deficit in blood being higher than the base deficit in extracellular fluid (A) and (B). With the use of receiver operating characteristic Curves in cohort 1, the area under curve to indicate a 5-minute Apgar score of < 7 and < 4 showed the area under curve-pH to be greater than the area under curve-base deficit in extracellular fluid (A) and (B), the area under curve-base deficit in blood to be greater than the area under Curve-base deficit in extracellular fluid (A) and (B) for a 5-minute Apgar score of < 7, and the area under curve-base deficit in blood to be greater than the area under curve-base deficit in extracellular fluid (A) and (B) for an Apgar score of < 4. The cutoffs with highest sensitivity and lowest false-positive rate for a 5-minute Apgar score of < 7 and < 4 were, for both scores, a pH value of 7.15, a base deficit in blood of 10 mmol/L, a base deficit in extracellular fluid (A) of 8 mmol/L, and a base deficit in extracellular fluid (B) of 6 mmol/L. Conclusion: The calculated values of the base deficit in umbilical cord arterial blood are influenced decisively by gestational age, the choice of fetal fluid compartment, and the calculation algorithms that are used. The power of the base deficit to indicate neonatal distress depends on the choices of fluid compartment and the algorithm that is used to calculate the base deficit. (c) 2006 Mosby, Inc. All rights reserved.
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| 22. |
- Wiberg, Nana, et al.
(författare)
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Delayed umbilical cord clamping at birth has effects on arterial and venous blood gases and lactate concentrations
- 2008
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Ingår i: BJOG: An International Journal of Obstetrics & Gynaecology. - : Wiley. - 1471-0528 .- 1470-0328. ; 115:6, s. 697-703
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Tidskriftsartikel (refereegranskat)abstract
- BJECTIVE: To estimate the influence of delayed umbilical cord clamping at birth on arterial and venous umbilical cord blood gases, bicarbonate (HCO3-), base excess (BE) and lactate in vigorous newborns. SETTING: University hospital. DESIGN: Prospective observational. SAMPLE: Vaginally delivered term newborns. MATERIAL AND METHODS: Umbilical cord arterial and venous blood was sampled repeatedly every 45 seconds (T(0)= time zero; T(45)= 45 seconds, T(90)= 90 seconds) until the cord pulsations spontaneously ceased in 66 vigorous singletons with cephalic vaginal delivery at 36-42 weeks. Longitudinal comparisons were performed with the Wilcoxon signed-ranks matched pairs test. Mixed effect models were used to describe the shape of the regression curves. MAIN OUTCOME MEASURES: Longitudinal changes of umbilical cord blood gases and lactate. RESULTS: In arterial cord blood, there were significant decreases of pH (7.24-7.21), HCO3- (18.9-18.1 mmol/l) and BE (-4.85 to -6.14 mmol/l), and significant increases of PaCO(2) (7.64-8.07 kPa), PO(2) (2.30-2.74 kPa) and lactate (5.3-5.9 mmol/l) from T(0) to T(90), with the most pronounced changes at T(0)-T(45). Similar changes occurred in venous blood pH (7.32-7.31), HCO3- (19.54-19.33 mmol/l), BE (-4.93 to -5.19 mmol/l), PaCO(2) (5.69-5.81 kPa) and lactate (5.0-5.3 mmol/l), although the changes were smaller and most pronounced at T(45)-T(90). No significant changes were observed in venous PO(2). CONCLUSION: Persistent cord pulsations and delayed cord clamping at birth result in significantly different measured values of cord blood acid-base parameters.
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| 23. |
- Wiberg, Nana, et al.
(författare)
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Lactate concentration in umbilical cord blood is gestational age-dependent: a population-based study of 17 867 newborns.
- 2008
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Ingår i: BJOG: An International Journal of Obstetrics & Gynaecology. - : Wiley. - 1471-0528 .- 1470-0328. ; 115:6, s. 704-709
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To study the influence of gestational age on lactate concentration in arterial and venous umbilical cord blood at birth and to define gestational age-specific reference values for lactate in vigorous newborns. DESIGN: Population-based comparative. SETTING: University hospitals. SAMPLE: Vigorous newborns with validated umbilical cord blood samples. MATERIAL AND METHODS: From 2000 to 2004, routine cord blood gases, lactate and obstetric data from two university hospitals were available for 17 867 newborns from gestational week 24 to 43. After validation of blood samples and inclusion only of singleton pregnancies aimed for vaginal delivery, 10 700 women remained. Among those, reference values were defined in 10 169 vigorous newborns, that is in newborns with a 5-minute Apgar score corresponding to the gestational age-specific median value minus 1 point score, or better. MAIN OUTCOME MEASURES: Cord lactate concentration relative to gestational age. RESULTS: The arterial and venous lactate concentrations increased monotonously with gestational age from 34 weeks. Considerable differences were found between mean and median values, but after logarithmic transformation the log-lactate values were normally distributed. Simple linear regression analysis showed a significant association between the log-lactate values and gestational age (P < 10(-6), R(2)= 0.024). Reference curves were constructed after anti-logarithmic transformation. Both the gestational age and the time of the second stage of labour influenced, independently of each other, the lactate concentrations. CONCLUSIONS: Lactate concentrations in arterial and venous umbilical cord blood are increasing significantly with advancing gestational age.
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| 24. |
- Wiberg, Nana, et al.
(författare)
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Physiological development of a mixed metabolic and respiratory umbilical cord blood acidemia with advancing gestational age.
- 2006
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Ingår i: Early Human Development. - : Elsevier BV. - 1872-6232 .- 0378-3782. ; 82:9, s. 583-589
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To investigate respiratory and metabolic components of gestational age-dependent umbilical cord blood gas changes. Study design: Cord blood gases were determined in 1336 vigorous singletons with uncomplicated cephatic vaginal delivery at 37-43 weeks. Linear regression analysis and non-parametric statistics were used with a P < 0.05 being significant. Results: Gestational age correlated negatively with arterial pH and HCO3- and positively with pCO(2) and base deficit. Venous blood showed corresponding changes except for pCO(2), which was independent of gestational age. Arterial pCO(2), but not venous, correlated positively with birthweight deviation from the mean. The fractional fetal CO2 production per birthweight unit and the fractional placental CO2 clearance per placental weight unit were not correlated with gestational age. Conclusions: A mixed respiratory and metabolic cord blood acidemia develops with advancing gestational age. The respiratory component depends on an increased 'CO2 load' fetus and not on deterioration of placental gas exchange. The etiology of the metabolic component is yet unknown. (c) 2006 Published by Elsevier Ireland Ltd.
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| 25. |
- Åhs, Margareta, et al.
(författare)
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Gymnasieskolans kursprov vt 2007 : En resultatredovisning
- 2008
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Rapport (populärvet., debatt m.m.)abstract
- Proven har hög legitimitet hos lärarna. Kraven stämmer till stora delar överens med lärarnassynpunkter och proven anses matcha lärarnas undervisning.Ungefär 20 procent av eleverna får ett högre preliminärt kursbetyg än provbetyg. Liksomi tidigare undersökningar finns indikationer om att främst kvinnorna lyckas höja sitt betygnär lärare tar hänsyn till annat betygsunderlag än provet.Engelska A och B har jämfört med andra ämnen lägsta andelen (ca 4%) elever med IG iprovbetyg . För Matematik B och C är motsvarande andel ca 20 procent.Engelska B och Matematik D har de största andelarna elever med provbetyget MVG.Många elever på de yrkesinriktade programmen uppnår inte godkänt resultat på proven.Dock är variationen stor mellan programmen.Generellt kan man se att elever med annat modersmål har svårare att hävda sig på proven.Dock tycks denna elevgrupp lyckas bättre i de muntliga delarna av kursproven.Liksom när det gäller det sammanräknade provbetyget i Engelska A lyckades eleverna påde naturvetenskapliga, samhällsvetenskapliga och tekniska programmen tillsammans medeleverna på det estetiska programmet bäst med de receptiva delproven, medan andelen ickegodkända resultat var stor på flera av de yrkesinriktade programmen.Rent allmänt visade kursproven i engelska att svarsformer av matchningskaraktär ellerflervalstyp är till männens fördel. Kvinnor lyckas däremot bättre på provdelar med övervägandeegenproducerade svar.Nyordningen med att ge kursprovet i Matematik A på två dagar istället för en pekar resultatmässigtpå att det blivit färre elever med IG som provbetyg. Förklaringen kan vara attelever som har låg uthållighet ges större chans att orka med hela provet. Rent allmänt kanman konstatera att eleverna på de yrkesförberedande programmen lyckats påfallande bättreän tidigare år med provet i Matematik A. Samma trend, fast något svagare, gäller även proveti Matematik B.Flera lärare i Svenska B påpekar att provet var väl anpassat för de program de undervisarpå. Kanske är det ett resultat av att provkonstruktörerna fördjupat sig i frågan vad som gynnaroch missgynnar i proven utifrån program. Värt att notera när det gäller resultaten är attför första gången är G det vanligaste betyget på den långa skrivuppgiften också för kvinnligaelever medan VG fortfarande är det vanligaste provbetyget hos kvinnliga elever.
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| 26. |
- Åström-Olsson, Karin, 1959, et al.
(författare)
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Myocardial release of FKBP12 and increased production of FKBP12.6 in ischemia and reperfusion experimental models
- 2009
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Ingår i: Biochem Biophys Res Commun. - : Elsevier BV. - 1090-2104 .- 0006-291X. ; 390:4, s. 1299-304
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Coronary artery occlusion and reperfusion may trigger reversible and irreversible ischemic and reperfusion injury. The primary aim of this study was to evaluate protein release into the myocardium in a porcine model during ischemia and reperfusion to search for clarifying models for reperfusion injury and secondarily to investigate release and production of the immunophilins FKBP12/12.6 in this model and in cell cultures. METHODS: In a porcine model local myocardial ischemia was induced during 45min followed by 120min of reperfusion. Microdialysis samples from ischemic and non-ischemic areas were analyzed with surface-enhanced laser desorption ionization (SELDI) mass spectrometry (MS) and Western blotting (WB). Myocardial biopsies from areas at risk and control areas were analyzed with reverse transcription polymerase chain reaction (RT-PCR). Myocardial cell cultures from mice (HL-1 cells) were exposed to hypoxia and then analyzed with WB and RT-PCR. RESULTS: FK binding protein12 (FKBP12), ubiquitin and myoglobin were identified as being released during ischemia and reperfusion in microdialysates. RT-PCR analysis on the biopsies after ischemia revealed a non-significant increase in mRNA expression of FKBP12 and a significant increase in mRNA expression of FKBP12.6. Lysates from HL-1 cells exposed to hypoxia demonstrated increase of FKBP12 and a significant increase in mRNA expression of FKBP12.6. CONCLUSION: In a myocardial ischemic-reperfusion porcine model as well as in hypoxic HL-1 cells, release of FKBP12 and increased production of FKBP12.6 was demonstrated. The findings indicate important mechanisms related to these immunophilins in the reaction to ischemia/hypoxia and reperfusion in the heart.
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