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Method development ...
Method development and validation for the quantitation of the complement inhibitor Cp40 in human and cynomolgus monkey plasma by UPLC-ESI-MS
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- Primikyri, Alexandra (författare)
- Univ Penn, Dept Pathol & Lab Med, 401 Stellar Chance,422 Curie Blvd, Philadelphia, PA 19104 USA.
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- Papanastasiou, Malvina (författare)
- Univ Penn, Dept Pathol & Lab Med, 401 Stellar Chance,422 Curie Blvd, Philadelphia, PA 19104 USA.
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- Sarigiannis, Yiannis (författare)
- Univ Penn, Dept Pathol & Lab Med, 401 Stellar Chance,422 Curie Blvd, Philadelphia, PA 19104 USA.
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- Koutsogiannaki, Sophia (författare)
- Univ Penn, Dept Pathol & Lab Med, 401 Stellar Chance,422 Curie Blvd, Philadelphia, PA 19104 USA.
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- Reis, Edimara S. (författare)
- Univ Penn, Dept Pathol & Lab Med, 401 Stellar Chance,422 Curie Blvd, Philadelphia, PA 19104 USA.
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- Tuplano, Joel V. (författare)
- Simian Conservat Breeding & Res Ctr SICONBREC, Makati, Philippines.
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- Resuello, Ranillo R. G. (författare)
- Simian Conservat Breeding & Res Ctr SICONBREC, Makati, Philippines.
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- Nilsson, Bo (författare)
- Uppsala universitet,Klinisk immunologi
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- Ricklin, Daniel (författare)
- Univ Penn, Dept Pathol & Lab Med, 401 Stellar Chance,422 Curie Blvd, Philadelphia, PA 19104 USA.;Univ Basel, Dept Pharmaceut Sci, Klingelbergstr 50, CH-4056 Basel, Switzerland.
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- Lambris, John D. (författare)
- Univ Penn, Dept Pathol & Lab Med, 401 Stellar Chance,422 Curie Blvd, Philadelphia, PA 19104 USA.
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Univ Penn, Dept Pathol & Lab Med, 401 Stellar Chance,422 Curie Blvd, Philadelphia, PA 19104 USA Simian Conservat Breeding & Res Ctr SICONBREC, Makati, Philippines. (creator_code:org_t)
- Elsevier BV, 2017
- 2017
- Engelska.
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Ingår i: Journal of chromatography. B. - : Elsevier BV. - 1570-0232 .- 1873-376X. ; 1041, s. 19-26
- Relaterad länk:
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http://manuscript.el...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Cp40 is a 14-amino acid cyclic analog of the peptidic complement inhibitor compstatin that binds with sub-nanomolar affinity to complement component C3 and has already shown promise in various models of complement-related diseases. The preclinical and clinical development of this compound requires a robust, accurate, and sensitive method for quantitatively monitoring Cp40 in biological samples. In this study, we describe the development and validation of an ultra-high performance liquid chromatography electrospray mass spectrometry method for the quantitation of Cp40 in human and non-human primate (NHP) plasma. Isotope-labeled Cp40 was used as an internal standard, allowing for the accurate and absolute quantitation of Cp40. Labeled and non-labeled Cp40 were extracted from plasma using reversed phase-solid phase extraction, with recovery rates exceeding 80%, indicating minor matrix effects. The triply charged states of Cp40 and isotope-labeled Cp40 were detected at m/z 596.60 and 600.34, respectively, via a Q-TOF mass spectrometer and were used for quantitation. The method was linear in the range of 0.18-3.58 mu g/mL (r(2) >= 0.99), with precision values below 0.71% in NHP and 0.77% in human plasma. The accuracy of the method ranged from -2.17% to 17.99% in NHP and from -0.26% to 15.75% in 'human plasma. The method was successfully applied to the quantitation of Cp40 in cynomolgus monkey plasma after an initial intravenous bolus of 2 mg/kg followed by repetitive subcutaneous administration at 1 mg/kg. The high reproducibility, accuracy, and robustness of the method developed here render it suitable for drug monitoring of Cp40, and potentially other compstatin analogs, in both human and NHP plasma samples during pharmacokinetic and pharmacodynamic studies.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Compstatin
- Absolute quantitation
- UPLC-ESI-MS
- Method validation
- Plasma
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Primikyri, Alexa ...
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Papanastasiou, M ...
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Sarigiannis, Yia ...
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Koutsogiannaki, ...
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Reis, Edimara S.
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Tuplano, Joel V.
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Resuello, Ranill ...
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Nilsson, Bo
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Ricklin, Daniel
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Lambris, John D.
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Uppsala universitet