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Sökning: WFRF:(Patriarca Claudia) > (2021)

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1.
  • Patriarca, Claudia, et al. (författare)
  • Character and environmental lability of cyanobacteria-derived dissolved organic matter
  • 2021
  • Ingår i: Limnology and Oceanography. - : Wiley. - 0024-3590 .- 1939-5590. ; 66:2, s. 496-509
  • Tidskriftsartikel (refereegranskat)abstract
    • Autotrophic dissolved organic matter (DOM) is central to the carbon biogeochemistry of aquatic systems, and the full complexity of autotrophic DOM has not been extensively studied, particularly by high-resolution mass spectrometry (HRMS). Terrestrial DOM tends to dominate HRMS studies in freshwaters due to the propensity of such compounds to ionize by negative mode electrospray, and possibly also because ionizable DOM produced by autotrophy is decreased to low steady-state concentrations by heterotrophic bacteria. In this study, we investigated the character of DOM produced by the widespread cyanobacteria Microcystis aeruginosa using high-pressure liquid chromatography—electrospray ionization—high-resolution mass spectrometry. M. aeruginosa produced thousands of detectable compounds in axenic culture. These compounds were chromatographically resolved and the majority were assigned to aliphatic formulas with a broad polarity range. We found that the DOM produced by M. aeruginosa was highly susceptible to removal by heterotrophic freshwater bacteria, supporting the hypothesis that this autotroph-derived organic material is highly labile and accordingly only seen at low concentrations in natural settings. 
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2.
  • Patriarca, Claudia, et al. (författare)
  • High Molecular Weight Spectral Interferences in Mass Spectra of Dissolved Organic Matter
  • 2021
  • Ingår i: Journal of the American Society for Mass Spectrometry. - : American Chemical Society (ACS). - 1044-0305 .- 1879-1123. ; 32:1, s. 394-397
  • Tidskriftsartikel (refereegranskat)abstract
    • In this paper we investigate interferences that appear in molecular mass spectra from aquatic samples. The interferences are identified as doubly charged ions originating from high molecular weight material, which is especially abundant in terrestrial samples. The interferences could be incorrectly assigned to singly charged formulas with high aromaticity and heteroatom content, as the mass error from such formulas can be less than 1 ppm. We propose a strategy for filtering the interference peaks from mass lists based on the presence of their equivalent isotopologue peaks at mass defects of similar to 0.5 Da.
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