Sökning: WFRF:(Rodriguez Fontenla Cristina)
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Synaptic inputs fro...
Synaptic inputs from stroke-injured brain to grafted human stem cell-derived neurons activated by sensory stimuli
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- Tornero, Daniel (författare)
- Lund University,Lunds universitet,Stem Cells & Restorative Neurology,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Tsupykov, Oleg (författare)
- Bogomoletz Institute of Physiology
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- Granmo, Marcus (författare)
- Lund University,Lunds universitet,Neuronano Research Center (NRC),Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Rodriguez-Fontenla, Cristina (författare)
- Lund University
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- Grønning-Hansen, Marita (författare)
- Lund University,Lunds universitet,Stem Cells & Restorative Neurology,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Thelin, Jonas (författare)
- Lund University,Lunds universitet,Neuronano Research Center (NRC),Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Smozhanik, Ekaterina (författare)
- Bogomoletz Institute of Physiology
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- Laterza, Cecilia (författare)
- Lund University,Lunds universitet,Stem Cells & Restorative Neurology,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Wattananit, Somsak (författare)
- Lund University,Lunds universitet,Stem Cells & Restorative Neurology,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Ge, Ruimin (författare)
- Lund University,Lunds universitet,Stem Cells & Restorative Neurology,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Tatarishvili, Jemal (författare)
- Lund University,Lunds universitet,Stem Cells & Restorative Neurology,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Grealish, Shane (författare)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups
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- Brüstle, Oliver (författare)
- University of Bonn
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- Skibo, Galina (författare)
- Bogomoletz Institute of Physiology
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- Parmar, Malin (författare)
- Lund University,Lunds universitet,Utvecklings- och regenerativ neurobiologi,Forskargrupper vid Lunds universitet,Developmental and Regenerative Neurobiology,Lund University Research Groups
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- Schouenborg, Jens (författare)
- Lund University,Lunds universitet,Neuronano Research Center (NRC),Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Lindvall, Olle (författare)
- Lund University,Lunds universitet,Stem Cells & Restorative Neurology,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Kokaia, Zaal (författare)
- Lund University,Lunds universitet,Stem Cells & Restorative Neurology,Forskargrupper vid Lunds universitet,Lund University Research Groups
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(creator_code:org_t)
- 2017-01-22
- 2017
- Engelska 15 s.
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Ingår i: Brain. - : Oxford University Press (OUP). - 0006-8950 .- 1460-2156. ; 140:3, s. 692-706
- Relaterad länk:
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http://dx.doi.org/10...
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visa fler...
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https://academic.oup...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Transplanted neurons derived from stem cells have been proposed to improve function in animal models of human disease by various mechanisms such as neuronal replacement. However, whether the grafted neurons receive functional synaptic inputs from the recipient's brain and integrate into host neural circuitry is unknown. Here we studied the synaptic inputs from the host brain to grafted cortical neurons derived from human induced pluripotent stem cells after transplantation into stroke-injured rat cerebral cortex. Using the rabies virus-based trans-synaptic tracing method and immunoelectron microscopy, we demonstrate that the grafted neurons receive direct synaptic inputs from neurons in different host brain areas located in a pattern similar to that of neurons projecting to the corresponding endogenous cortical neurons in the intact brain. Electrophysiological in vivo recordings from the cortical implants show that physiological sensory stimuli, i.e. cutaneous stimulation of nose and paw, can activate or inhibit spontaneous activity in grafted neurons, indicating that at least some of the afferent inputs are functional. In agreement, we find using patch-clamp recordings that a portion of grafted neurons respond to photostimulation of virally transfected, channel-rhodopsin-2-expressing thalamo-cortical axons in acute brain slices. The present study demonstrates, for the first time, that the host brain regulates the activity of grafted neurons, providing strong evidence that transplanted human induced pluripotent stem cell-derived cortical neurons can become incorporated into injured cortical circuitry. Our findings support the idea that these neurons could contribute to functional recovery in stroke and other conditions causing neuronal loss in cerebral cortex.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Nyckelord
- Functional integration
- Stem cells
- Stroke
- Synapses
- Transplantation
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
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Brain
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- Av författaren/redakt...
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Tornero, Daniel
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Tsupykov, Oleg
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Granmo, Marcus
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Rodriguez-Fonten ...
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Grønning-Hansen, ...
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Thelin, Jonas
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visa fler...
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Smozhanik, Ekate ...
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Laterza, Cecilia
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Wattananit, Soms ...
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Ge, Ruimin
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Tatarishvili, Je ...
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Grealish, Shane
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Brüstle, Oliver
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Skibo, Galina
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Parmar, Malin
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Schouenborg, Jen ...
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Lindvall, Olle
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Kokaia, Zaal
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och Neurovetenskaper
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Neurologi
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