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| 2. |
- Gellein, Kristin, et al.
(författare)
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Separation of proteins including metallothionein in cerebrospinal fluid by size exclusion HPLC and determination of trace elements by HR-ICP-MS
- 2007
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Ingår i: Brain Research. - : Elsevier BV. - 0006-8993 .- 1872-6240. ; 1174, s. 136-142
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Tidskriftsartikel (refereegranskat)abstract
- A method to study the protein binding patterns of trace elements in human cerebrospinal fluid (CSF) is described. Proteins in CSF samples were separated by size exclusion chromatography combined with high performance liquid chromatography (SEC-HPLC). The column was calibrated to separate proteins in the molecular weight range 6-70 kDa. Fractions were then analyzed off-line for trace elements using high resolution inductively coupled plasma mass spectrometry (HR-ICP-MS). We were able to accurately determine more than 10 elements of clinical interest in the CSF fractions. Results are presented for Cd, Mn, Fe, Pb, Cu and Zn. The total concentrations of 16 trace elements in human plasma and CSF are also presented. The method was able to differentiate the relative contribution of metallothionein and other proteins towards metal binding in human CSF.
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| 3. |
- Hammarström, Per, 1960-
(författare)
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Nationens styvbarn : Judisk samhällsintegration i några Norrlandsstäder 1870-1940
- 2007
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Ingår i: Svensk kyrkotidning. - Stockholm : Carlsson Bokförlag. - 0346-2153. ; :51-52, s. 664-
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Syftet med denna avhandling är att undersöka vilken ekonomisk och social ställning den judiska minoriteten intog i det svenska samhället under perioden 1870 till 1940. Tidigare forskning har riktat stor uppmärksamhet mot rättsliga och politiska aspekter av den svenska judenhetens historia, mer sällan mot judarna och samhället i en bredare bemärkelse. Andra studier har lyft fram antisemitism på svensk mark utan att koppla fenomenet till en social nivå.Genom en undersökning av hur judiska invandrare, med ursprung i Östeuropa, successivt fogades in i några svenska lokalsamhällen, tar denna avhandling ett bredare grepp på frågan om judarna och samhället. Här undersöks hur den svenska judenheten integrerades i samhället efter det att emancipationen gett judarna fulla medborgerliga och politiska rättigheter. Judarnas ekonomiska och sociala ställning undersöks, liksom den sociala antisemitism som drabbade judarna. Studien rör sig i gränslandet mellan social- och kulturhistoria. Undersökningen visar att merparten av judarna i Sundsvall, en betydelsefull handelsstad i norra Sverige, fick sin försörjning genom en föga lönsam gårdfarihandel under början av undersökningsperioden. Sedan butiks- och grosshandelsrörelser började grundas inleddes en långsam socioekonomisk klättring. I takt med de ekonomiska framgångarna ökade judarnas sociala status, med engagemang i föreningslivet och lokalpolitiken. De framgångsrika familjerna tonade ner sin judiska identitet till förmån för en borgerlig och nationell kultur.Ungefär hälften av den judiska gruppen fick aldrig uppleva framgången utan förblev en marginaliserad grupp, med småhantverk eller gårdfarihandel som försörjningsbas. Det stora flertalet av denna underklass saknade svenskt medborgarskap. En social klyfta vidgades gentemot den judiska borgerligheten. Under hela undersökningsperioden var antisemitismen en kulturell kod i majoritetssamhället, oavsett integration och socioekonomisk framgång. Mycket tyder på att det sociala klimatet blev mer fientligt gentemot judarna efter 1920. Studien visar att nationalstaten Sverige lämnade litet utrymme för multikulturella strukturer. Judarna accepterades som nationens styvbarn men knappast som fullvärdiga svenskar.
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| 4. |
- Masso, H., et al.
(författare)
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Ab initio characterization of C-5
- 2007
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Ingår i: Journal of Chemical Physics. - : AIP Publishing. - 0021-9606 .- 1089-7690. ; 127:15
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Tidskriftsartikel (refereegranskat)abstract
- In this paper, the structure and spectroscopic parameters of the C-5 cluster are determined using multiconfigurational quantum chemical methods as implemented in the MOLCAS software. A number of spectroscopic properties (band center positions, l-doubling parameters, and rotational constants) have been characterized. From the new results, the assignments of previous astrophysical observations [J. Goicoechea , Astrophys. J. 609, 225 (2004)] are discussed. A detailed exploration of the global potential energy surface confirms that C-5 has a X (1)Sigma(+)(g) linear isomer of prominent stability and, at least, three minimum energy structures showing singlet electronic ground states. Two of them are cyclic and one has a nonplanar geometry. Vertical and adiabatic electronic transitions and vibrational spectroscopic parameters are determined for the most stable linear isomer using multiconfigurational second order perturbation theory (CASPT2) using an active space containing 12 valence orbitals with 12 active electrons and extended ANO-type basis sets. The infrared spectrum has been analyzed from an anharmonic force field derived form the local surface, determined from the energies of a grid of 1350 geometries. The force field includes four coupling terms. The CASPT2 band center position of the nu(7)(pi(u)) anharmonic fundamental has been calculated to be at 102 cm(-1), which validates the assignment to C-5 of the pattern of bands centered at 102 cm(-1) observed with the ISO telescope. (C) 2007 American Institute of Physics.
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| 6. |
- Petersén, Åsa, et al.
(författare)
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Orexin loss in Huntington's disease.
- 2005
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Ingår i: Human Molecular Genetics. - : Oxford University Press (OUP). - 0964-6906 .- 1460-2083. ; 14:1, s. 39-47
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Tidskriftsartikel (refereegranskat)abstract
- Huntington's disease (HD) is a devastating neurodegenerative disorder caused by an expanded CAG repeat in the gene encoding huntingtin, a protein of unknown function. Mutant huntingtin forms intracellular aggregates and is associated with neuronal death in select brain regions. The most studied mouse model (R6/2) of HD replicates many features of the disease, but has been reported to exhibit only very little neuronal death. We describe for the first time a dramatic atrophy and loss of orexin neurons in the lateral hypothalamus of R6/2 mice. Importantly, we also found a significant atrophy and loss of orexin neurons in Huntington patients. Like animal models and patients with impaired orexin function, the R6/2 mice were narcoleptic. Both the number of orexin neurons in the lateral hypothalamus and the levels of orexin in the cerebrospinal fluid were reduced by 72% in end-stage R6/2 mice compared with wild-type littermates, suggesting that orexin could be used as a biomarker reflecting neurodegeneration. Our results show that the loss of orexin is a novel and potentially very important pathology in HD.
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| 7. |
- Roos, Katarina, 1982-, et al.
(författare)
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Density Functional Theory Study of the Manganese-Containing Ribonucleotide Reductase from Chlamydia trachomatis : Why Manganese Is Needed in the Active Complex
- 2009
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Ingår i: Biochemistry. - : American Chemical Society (ACS). - 0006-2960 .- 1520-4995. ; 48:9, s. 1878-1887
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Tidskriftsartikel (refereegranskat)abstract
- The active center of Chlamydia trachomatis (Ct) ribonucleotide reductase (RNR) has been studied using B3LYP hybrid density functional theory. Class Ic Ct RNR lacks the radical-bearing tyrosine that is crucial for activity in conventional class I (subclass a and b) RNR. Instead of the Fe(III)Fe(III)Tyr(rad) active state in conventional class I, Ct RNR has Mn(IV)Fe(III) at the metal center of subunit H. Based on calculated (H+, e(-))-binding energies for Ct R2, iron-substituted Ct R2, and R2 from Escherichia coli (Ec), an explanation is proposed for why the enzyme needs this novel metal center. Mn(IV) is shown to be an equally strong oxidant as the tyrosyl radical in Ec R2. Fe(IV), however, is a much too strong oxidant and would therefore not be possible in the active cofactor. The structure of the catalytic center of the active state, such as protonation state and position of Mn, is discussed. Ct R2 has a different ligand structure than conventional class I R2 with a fourth Glu (like MMO) instead of three Glu and one Asp. Calculations indicate that, in the presence of Tyr, Glu at this position is less flexible than Asp, whereas with Phe both Glu and Asp are equally flexible. This may be a reason why conventional class I RNR has an Asp, while Ct R2, lacking the tyrosine, has a Glu.
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