An OregonGreen488-labelled d-amino acid for visualizing peptidoglycan by super-resolution STED nanoscopy

• Fluorescent o amino acids (FDAAs) are molecular probes that are widely used for labelling the peptidoglycan layer of bacteria. When added to growing cells they are incorporated into the stem peptide by a transpeptidase reaction, allowing the timing and localization of peptidoglycan synthesis to be determined by fluorescence microscopy. Herein we describe the chemical synthesis of an Oregon Green 488 labelled FDAA (OGDA). We also demonstrate that OGDA can be efficiently incorporated into the PG of Grampositive and some Gramnegative bacteria, and imaged by superresolution stimulated emission depletion (STED) nanoscopy at a resolution well below 100 nm.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

FDAA

FLDA

OGDA

OregonGreen488

peptidoglycan

transpeptidase

Publikations- och innehållstyp

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