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A DNA piezoelectric...
A DNA piezoelectric biosensor assay coupled with a polymerase chain reaction for bacterial toxicity determination in environmental samples
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- Tombelli, S (författare)
- Sez Chim Analit, Dipartimento Sanita Pubbl Epidemiol and Chim Analit, Florence, Italy; Sez Igiene, Dipartimento Sanita Pubbl Epidemiol and Chim Analit, Florence, Italy; Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;
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- Mascini, M (författare)
- Sez Chim Analit, Dipartimento Sanita Pubbl Epidemiol and Chim Analit, Florence, Italy; Sez Igiene, Dipartimento Sanita Pubbl Epidemiol and Chim Analit, Florence, Italy; Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;
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- Sacco, C (författare)
- Sez Chim Analit, Dipartimento Sanita Pubbl Epidemiol and Chim Analit, Florence, Italy; Sez Igiene, Dipartimento Sanita Pubbl Epidemiol and Chim Analit, Florence, Italy; Cranfield University, Cranfield Biotechnol Centre, Cranfield MK43 0AL, Beds, England;
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visa fler...
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- Turner, APF (författare)
- Cranfield University, UK
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visa färre...
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(creator_code:org_t)
- Elsevier Science B.V., Amsterdam. 2000
- 2000
- Engelska.
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Ingår i: Analytica Chimica Acta. - : Elsevier Science B.V., Amsterdam.. - 0003-2670 .- 1873-4324. ; 418:1
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- In this paper, we report the realisation of a DNA piezoelectric biosensor coupled with the polymerase chain reaction (PCR) for the detection of a specific bacterial toxicity factor. Biotinylated 23-mer probes were immobilised on the streptavidin coated gold surface of a quartz crystal; streptavidin was covalently bound to the thiol/dextran modified gold surface. The hybridisation of the immobilised probe with a synthetic oligonucleotide was investigated; the absence of non-specific adsorption was verified using a non-complementary oligonucleotide. Many cycles of measurements can be performed on the same crystal surface by regenerating the single strand with 1 mM HCl. The same hybridisation reaction was then performed using real samples of DNA extracted from bacteria and amplified by PCR. The PCR product was a fragment of a specific gene of Aeromonas hydrophila. The piezoelectric biosensor was able to distinguish samples containing the gene or not; in this way it was possible to determine the pathogenicity of different Aeromonas strains isolated from water, vegetables or human specimens. Experiments with non-specific samples confirmed the absence of adsorption or non-specific effects on the quartz crystal treated with the reported procedure. (C) 2000 Elsevier Science B.V. All rights reserved.
Nyckelord
- DNA piezoelectric biosensor; polymerase chain reaction; bacterial toxicity; hybridisation
- TECHNOLOGY
- TEKNIKVETENSKAP
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