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Toward Complete Seq...
Toward Complete Sequence Flexibility of Nucleic Acid Base Analogue FRET
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- Sandberg Wranne, Moa, 1986 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Füchtbauer, Anders Foller, 1984 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Dumat, Blaise, 1984 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Bood, Mattias (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg,AstraZeneca AB
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- El-Sagheer, A. H. (författare)
- University Of Oxford,Suez University
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- Brown, T. (författare)
- University Of Oxford
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- Gradén, Henrik, 1968 (författare)
- AstraZeneca AB
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- Grötli, Morten (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
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- Wilhelmsson, Marcus, 1974 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- 2017-06-27
- 2017
- Engelska.
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 139:27, s. 9271-9280
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Abstract
Ämnesord
Stäng
- Forster resonance energy transfer (FRET) using fluorescent base analogues is a powerful means of obtaining high-resolution nucleic acid structure and dynamics information that favorably complements techniques such as NMR and X-ray crystallography. Here, we expand the base base FRET repertoire with an adenine analogue FRET-pair. Phosphoramidite-protected quadracyclic 2'-deoxyadenosine analogues qAN1 (donor) and qA(nitro) (acceptor) were synthesized and incorporated into DNA by a genetic, reliable, and high-yielding route, and both constitute excellent adenine analogues. The donor, qAN1, has quantum yields reaching 21% and 11% in single- and double-strands, respectively. To the best of our knowledge, this results in the highest average brightness of an adenine analogue inside DNA. Its potent emissive features overlap well with the absorption of qA(nitro), and thus enable accurate FRET-measurements over more than one turn of B-DNA. As we have shown previously for our cytosine analogue FRET-pair, FRET between qAN1 and qA(nitro) positioned at different base separations inside DNA results in efficiencies that are highly dependent on both distance and orientation. This facilitates significantly enhanced resolution in FRET structure determinations, demonstrated here in a study of conformational changes of DNA upon binding of the minor groove binder netropsin. Finally, we note that the donor and acceptor, of our cytosine FRET-pair, tC degrees and tC(nitro), can be conveniently combined with the acceptor and donor of our current adenine pair, respectively. Consequently, our base analogues can now measure base base FRET between 3 of the 10 possible base combinations and, through base-complementarity, between all sequence positions in a duplex.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
Nyckelord
- FLUORESCENT ADENINE ANALOG
- PTERIDINE NUCLEOSIDE ANALOGS
- VERSATILE
- PROTECTING GROUP
- EMISSIVE RNA ALPHABET
- SINGLE-MOLECULE FRET
- QUADRACYCLIC ADENINE
- CIRCULAR-DICHROISM
- BUTYLDIMETHYLSILYLOXYMETHYL
- GROUP
- QUENCHING EFFICIENCIES
- ENERGY-TRANSFER
- Chemistry
- Multidisciplinary
- ATES OF AMERICA
- V105
- P18337
- RYER L
- 1967
- PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF
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Sandberg Wranne, ...
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Füchtbauer, Ande ...
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Dumat, Blaise, 1 ...
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Bood, Mattias
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El-Sagheer, A. H ...
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Brown, T.
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Gradén, Henrik, ...
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Grötli, Morten
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Wilhelmsson, Mar ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Andra medicinska ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Fysikalisk kemi
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Journal of the A ...
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Göteborgs universitet
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Chalmers tekniska högskola