| 1. |
- Sun, Chengjun, et al.
(författare)
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CRYAB-650 C>G (rs2234702) affects susceptibility to type 1 diabetes and IAA-positivity in Swedish population
- 2012
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Ingår i: Human Immunology. - : Elsevier. - 0198-8859 .- 1879-1166. ; 73:7, s. 759-766
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Single nucleotide polymorphisms (SNPs) in the promoter region of CRYAB gene have been associated with in multiple sclerosis. CRYAB gene, which encodes alpha B-crystallin (a member of small heat shock protein), was reported as a potential autoimmune target. In this study we investigated whether SNPs in the promoter region of CRYAB gene were also important in the etiology of Type 1 diabetes (T1D).METHODS: Genotyping of SNPs in the promoter region of CRYAB gene was performed in a Swedish cohort containing 444 T1D patients and 350 healthy controls. Three SNPs were included in this study: CRYAB-652 A>G (rs762550), -650 C>G (rs2234702) and -249 C > G (rs14133). Two SNPs (CRYAB-652 and -650) were not included in previous genome wide association studies.RESULTS: CRYAB-650 (rs2234702)*C allele was significantly more frequent in patients than in controls (OR = 1.48, Pc = 0.03). CRYAB-650*C allele was associated with IAA positivity (OR = 8.17, Pc < 0.0001) and IA-2A positivity (OR = 2.14, Pc = 0.005) in T1D patients. This association with IAA was amplified by high-risk HLA carrier state (OR = 10.6, P < 0.0001). No association was found between CRYAB-650 and other autoantibody positivity (GADA and ICA). CRYAB haplotypes were also associated with IAA and IA-2A positivity (highest OR = 2.07 and 2.11, respectively), these associations remain in high HLA-risk T1D patients.CONCLUSIONS: CRYAB-650 was associated with T1D in the Swedish cohort we studied. CRYAB-650*C allele might confers susceptibility to the development of T1D. CRYAB-650 was also associated with the development of IAA-positivity in T1D patients, especially in those carrying T1D high-risk HLA haplotypes.
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| 2. |
- Dahlström, Lisen Arnheim, et al.
(författare)
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Prospective study of human papillomavirus and risk of cervical adenocarcinoma.
- 2010
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Ingår i: International journal of cancer. Journal international du cancer. - : Wiley. - 1097-0215 .- 0020-7136. ; 127:8, s. 1923-30
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Tidskriftsartikel (refereegranskat)abstract
- Human papillomaviruses (HPV) are established as a major cause of cervical carcinoma. However, causality inference is dependent on prospective evidence showing that exposure predicts risk for future disease. Such evidence is available for squamous cell carcinoma, but not for cervical adenocarcinoma. We followed a population-based cohort of 994,120 women who participated in cytological screening in Sweden for a median of 6.7 years. Baseline smears from women who developed adenocarcinoma during follow-up (118 women with in situ disease and 164 with invasive disease) and their individually matched controls (1,434 smears) were analyzed for HPV using PCR. Conditional logistic regression was used to estimate odds ratios (OR) of future adenocarcinoma with 95% confidence intervals (CI). Being positive for HPV 16 in the first cytologically normal smear was associated with increased risks for both future adenocarcinoma in situ (OR: 11.0, 95% CI: 2.6-46.8) and invasive adenocarcinoma (OR: 16.0, 95% CI: 3.8-66.7), compared to being negative for HPV 16. Similarly, an HPV 18 positive smear was associated with increased risks for adenocarcinoma in situ (OR: 26.0, 95% CI: 3.5-192) and invasive adenocarcinoma (OR: 28.0, 95% CI: 3.8-206), compared to an HPV 18 negative smear. Being positive for HPV 16/18 in 2 subsequent smears was associated with an infinite risk of both in situ and invasive adenocarcinoma. In conclusion, infections with HPV 16 and 18 are detectable up to at least 14 years before diagnosis of cervical adenocarcinoma. Our data provide prospective evidence that the association of HPV 16/18 with cervical adenocarcinoma is strong and causal.
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| 3. |
- Skrodeniene, Erika, et al.
(författare)
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Associations between HLA class II haplotypes, environmental factors and type 1 diabetes mellitus in Lithuanian children with type 1 diabetes and controls
- 2010
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Ingår i: Polish Annals of Medicine. - Warsaw, Poland : Elsevier Urban & Partner Sp. z o.o.. - 1230-8013. ; 17:1, s. 7-15
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Tidskriftsartikel (refereegranskat)abstract
- Introduction. The onset of type 1 diabetes (T1D) is determined by genetic predisposition and environmental factors. Aim. The aim of our work was to identify associations between human leukocytes antigen (HLA) class II alleles, environmental factors and T1D in Lithuania. Materials and methods. Our case-control study included 124 diabetic children (mean age 9.19±3.94 years) and 78 controls (mean age 10.77±3.36 years). The age ranged from 0 to 15 years. HLA-DRB1, DQA1 and DQB1 alleles were genotyped using polymerase chain reaction. Information concerning the environmental factors was collected via questionnaires. Results. Logistic regression model indicated that three haplotypes: (DR3)-DQA1*0501-DQB1*0201, (DR4)-DQA1*0301-DQB1*0302 and (DR1)-DQA1 *010-04-DQB1*0501, increased the T1D risk statistically significantly 18.1, 12.3 and 3.4 times, respectively, while (DR11/12/13)-DQA1*05-DQB1*0301 haplotype decreased the risk of T1D 9.1 times. Several different regression models included environmental factors and different sets of risk and protective haplotypes. The results suggest that living in a remote area with lower population density during pregnancy increased the risk of T1D, as well as short breastfeeding, introduction of eggs before 5th month of age and infections during the last 6 months before diagnosis. Smoking during pregnancy as well as rubella and varicella virus infections seemed to decrease the risk of T1D. These associations were revealed while evaluating only environmental factors and when different HLA haplotypes together with environmental factors were included in the regression model. Discussion. The HLA typing shows that the differences in the incidence of T1D between Lithuania and neighboring countries cannot be explained only by genetics, but lifestyle and/or environmental factors should be considered. A number of studies presented here, have shown conflicting results regarding environmental factors and their associations with T1D. Conclusions. Both genetic and environmental factors play a major role in diabetes development and protection. However, even quite rapidly ongoing changes of environmental factors and lifestyle in Lithuania have not helped us to reveal any clear picture.
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| 4. |
- Sundström, Karin, et al.
(författare)
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Prospective study of human papillomavirus (HPV) types, HPV persistence, and risk of squamous cell carcinoma of the cervix.
- 2010
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Ingår i: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology. - 1538-7755 .- 1055-9965. ; 19:10, s. 2469-78
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Tidskriftsartikel (refereegranskat)abstract
- The link between squamous cell cervical carcinoma and human papillomavirus (HPV) 16/18 is well established, but the magnitude of the risk association is uncertain and the importance of other high-risk HPV (HRHPV) types is unclear.
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