| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
|
|
| 5. |
- Dias, Guilherme, 1989-, et al.
(författare)
-
Haplotype-resolved genome assembly enables gene discovery in the red palm weevil Rhynchophorus ferrugineus.
- 2021
-
Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 11:1, s. 9987-
-
Tidskriftsartikel (refereegranskat)abstract
- The red palm weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae) is an economically-important invasive species that attacks multiple species of palm trees around the world. A better understanding of gene content and function in R. ferrugineus has the potential to inform pest control strategies and thereby mitigate economic and biodiversity losses caused by this species. Using 10x Genomics linked-read sequencing, we produced a haplotype-resolved diploid genome assembly for R. ferrugineus from a single heterozygous individual with modest sequencing coverage ([Formula: see text] 62x). Benchmarking against conserved single-copy Arthropod orthologs suggests both pseudo-haplotypes in our R. ferrugineus genome assembly are highly complete with respect to gene content, and do not suffer from haplotype-induced duplication artifacts present in a recently published hybrid assembly for this species. Annotation of the larger pseudo-haplotype in our assembly provides evidence for 23,413 protein-coding loci in R. ferrugineus, including over 13,000 predicted proteins annotated with Gene Ontology terms and over 6000 loci independently supported by high-quality Iso-Seq transcriptomic data. Our assembly also includes 95% of R. ferrugineus chemosensory, detoxification and neuropeptide-related transcripts identified previously using RNA-seq transcriptomic data, and provides a platform for the molecular analysis of these and other functionally-relevant genes that can help guide management of this widespread insect pest.
|
|
| 6. |
- Dias, Guilherme, 1989-, et al.
(författare)
-
Complete mitochondrial genome of the longhorn date palm stem borer Jebusaea hammerschmidtii (Reiche, 1878).
- 2021
-
Ingår i: Mitochondrial DNA Part B. - : Informa UK Limited. - 2380-2359. ; 6:11, s. 3214-3216
-
Tidskriftsartikel (refereegranskat)abstract
- The 15,619 bp mitochondrial genome of Jebusaea hammerschmidtii was assembled from short reads, annotated, and compared to the genomes of other longhorn beetles (Cerambycidae). Gene content was typical of animal mitochondrial genomes and contained 13 protein-coding, 22 tRNA, and 2 rRNA genes. Gene organization was identical to that of other longhorn beetles. Phylogenetic analysis placed J. hammerschmidtii within the subfamily Cerambycinae, and strongly supported the monophyly of the Cerambycinae, Lamiinae, and Prioninae subfamilies.
|
|
| 7. |
- Manee, Manee M, et al.
(författare)
-
Omics in the Red Palm Weevil Rhynchophorus ferrugineus (Olivier) (Coleoptera : Curculionidae)
- 2023
-
Ingår i: Insects. - : MDPI AG. - 2075-4450. ; 14:3
-
Tidskriftsartikel (refereegranskat)abstract
- The red palm weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae), is the most devastating pest of palm trees worldwide. Mitigation of the economic and biodiversity impact it causes is an international priority that could be greatly aided by a better understanding of its biology and genetics. Despite its relevance, the biology of the RPW remains poorly understood, and research on management strategies often focuses on outdated empirical methods that produce sub-optimal results. With the development of omics approaches in genetic research, new avenues for pest control are becoming increasingly feasible. For example, genetic engineering approaches become available once a species's target genes are well characterized in terms of their sequence, but also population variability, epistatic interactions, and more. In the last few years alone, there have been major advances in omics studies of the RPW. Multiple draft genomes are currently available, along with short and long-read transcriptomes, and metagenomes, which have facilitated the identification of genes of interest to the RPW scientific community. This review describes omics approaches previously applied to RPW research, highlights findings that could be impactful for pest management, and emphasizes future opportunities and challenges in this area of research.
|
|
