| 1. |
- Ankerst, Jaro, et al.
(författare)
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Complete remission in a patient with acute myelogenous leukemia treated with leukocyte α-interferon and cimetidine
- 1984
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Ingår i: Cancer Immunology Immunotherapy. - 0340-7004. ; 17:1, s. 69-71
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Tidskriftsartikel (refereegranskat)abstract
- A 76-year-old woman with acute myelogenous leukemia with approximately 65% myeloblasts on bone marrow examination was treated daily with a combination of 4 megaU of leukocyte interferon IM and 1,000 mg cimetidine PO. During therapy there was a gradual decrease of bone marrow myeloblasts down to 9% and a normalization of peripheral white blood cells. The treatment was discontinued after 6 weeks because of increasing fatigue and anorexia. The general condition improved greatly during the following weeks and the patient entered complete remission, which has continued for 6 months so far. In the course of therapy there was a gradual appearance of antibodies showing a selective binding capacity to autochthonous leukemic cells with no tendency to increased binding to remission cells. The aim of this report is to stimulate a further evaluation of this form of therapy in additional AML patients whenever this might be justified as an alternative to conventional chemotherapy.
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| 2. |
- Malmström, P, et al.
(författare)
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ANAE staining pattern of rat lymphocytes : lack of correlation with lymphocyte subclasses
- 1981
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Ingår i: Scandinavian Journal of Immunology. - 0300-9475. ; 14:5, s. 7-523
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Tidskriftsartikel (refereegranskat)abstract
- Lymphocytes from various lymphoid organs of WF and BN rats were isolated and stained for acid alpha-naphthyl acetate esterase (ANAE) activity. From all organs examined-peripheral blood, lymph nodes, and spleen-70-80% of the lymphocytes were stained for ANAE activity. After separation into B and non-B cells by affinity chromatography on anti-Ig columns, esterase-negative lymphocytes were detected in both populations, demonstrating that ANAE staining cannot be used as a lymphocyte subclass marker in rats, in contrast to the situation in human and mice.
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| 3. |
- Malmström, P., et al.
(författare)
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Countercurrent Distribution of Lymphocytes from Human Peripheral Blood in an Aqueous Two-Phase System : I. Separation into Subsets of Lymphocytes Bearing Distinctive Markers’
- 1980
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Ingår i: Cellular Immunology. - : Elsevier BV. - 0008-8749. ; 53, s. 39-50
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Tidskriftsartikel (refereegranskat)abstract
- Lymphocytes from human peripheral blood have been separated by countercurrent distribution in a charged aqueous two-phase system composed of Dextran T 500 and polyethylene glycol 6000 with a cell yield of 59–88% and viability above 90%. A highly reproducible partition pattern was seen with four distinct peaks. Lymphocytes with surface membrane immunoglobulin (SmIg) were located in the first part of the distribution corresponding mainly to peak I. T lymphocytes as detected by E rosetting and α-naphthyl acetate esterase (ANAE) staining showed a broad distribution with a maximum in peaks II and III. ANAE-negative lymphocytes were seen in both extremes of the distribution, corresponding to B cells in the first part and to a population of E− and SmIg− lymphocytes in the last part. Monocytes were present in all fractions with some enrichment in peaks II–IV. Lymphocytes with low-affinity Fc receptors were found in B-cell-containing fractions in the first part of the distribution, but also in the last part. Lymphocytes with high-affinity Fc receptors were detected mainly in peak IV. It is thus demonstrated that peripheral blood lymphocytes can be fractionated into subpopulations enriched in cells with characteristic markers.markers.
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| 4. |
- Malmström, P, et al.
(författare)
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Countercurrent distribution of lymphocytes from human peripheral blood in an aqueous two-phase system. : II. Separation into subsets of lymphocytes with distinctive functions
- 1980
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Ingår i: Cellular Immunology. - : Elsevier BV. - 0008-8749. ; 53, s. 51-64
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Tidskriftsartikel (refereegranskat)abstract
- Lymphocytes from human peripheral blood were separated by Countercurrent distribution (CCD) in a charged aqueous two-phase system composed of Dextran T 500 and polyethylene glycol 6000. Maximal responses to the T-cell mitogens phytohemagglutinin and concanavalin A were detected in a part of the distribution corresponding to the area where the highest percentage of E rosetting cells were seen. Antibody-dependent cellular cytotoxicity was mediated by lymphocytes located in a restricted part of the distribution separate from the majority of T lymphocytes. Lymphocytes with natural killer activity against K 562, adherent melanoma cells, and fibroblasts were detected in the same region. This area of the distribution also contained the peak of lymphocytes with high-affinity Fc receptors. It was further investigated whether affinity separation may be possible in two-phase systems on the basis of cell to cell interaction. Affinity CCD utilizing the interaction between sheep erythrocytes (SRBC) and T lymphocytes was shown to redistribute the majority of T cells into the area in which SRBC were located, while other lymphocytes were not affected. Lymphocytes mediating natural killing to adherent target cells were not redistributed, indicating that they lack high-affinity receptors for SRBC.
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| 5. |
- Sjögren, H O, et al.
(författare)
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Column separation of monocytes by adherence to gelatin beads
- 1983
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Ingår i: Journal of Immunological Methods. - : Elsevier BV. - 0022-1759. ; 56:3, s. 285-294
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Tidskriftsartikel (refereegranskat)abstract
- This investigation was performed to study whether the efficient binding of collagen to monocytes in the presence of fibronectin and heparin may be used for separation of monocytes from human peripheral blood. It was shown that monocytes adhere selectively to gelatin bead columns in the presence of fresh plasma and heparin. Mononuclear blood cells are rapidly depleted of monocytes by passage through a 5-10 ml column at a flow rate of 1.5-2.0 ml per min. Adhering lymphocytes are more loosely attached and may be detached by stirring and washing, while the monocytes can be eluted by 50 mM EDTA. This separation technique is suitable for combination with various other methods since it is rapid, allows convenient handling of large numbers and yields cells with very high viability. Although most B lymphocytes pass through the column without attaching, there is some enrichment of B cells and non-T, non-B cells among the adherent lymphocytes.
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| 6. |
- Wallmark, A, et al.
(författare)
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Feasibility of extracorporeal on-line large-scale plasma adsorptions on protein A-sepharose columns in cancer patients
- 1984
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Ingår i: Artificial Organs. - : Wiley. - 0160-564X .- 1525-1594. ; 8:1, s. 72-81
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Tidskriftsartikel (refereegranskat)abstract
- The feasibility of extracorporeal adsorption of 1.5-3 L plasma on protein A-Sepharose was investigated in six patients with advanced cancer. Anticoagulation with heparin was associated with respiratory distress syndrome in two patients, most likely caused by complement activation as indicated by a transient leukopenia during plasma reinfusion and appearance of C3 degradation products in the extracorporeal circulation. Addition of citrate abolished the respiratory symptoms, C3 degradation, and leukopenia, and no adverse reactions were observed. No objective tumor regression was observed in any of the patients. Three patients progressed during therapy. In one of these, multifocal central tumor necrosis was observed as a possible, although unproven, therapeutic effect. Increased natural killer and/or killer cell activities were recorded in three patients and increased complement-dependent serum cytotoxicity in one patient. The level of circulating immune complexes decreased significantly (18-28%) in three patients studied. It is concluded that extracorporeal plasma adsorption on protein A-Sepharose is feasible when citrate is added to the extracorporeal system, but its therapeutic efficacy is uncertain.
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