Heparan sulfate synthesized by mouse embryonic stem cells deficient in NDST1 and NDST2 is 6-O-sulfated but contains no N-sulfate groups.

Nyckelord

Amidohydrolases/*genetics/physiology

Animals

Blastocyst/metabolism

Carbohydrate Epimerases/chemistry

Chromatography; High Pressure Liquid

Chromatography; Ion Exchange

DNA; Complementary/metabolism

Embryo/metabolism

Gene Expression Regulation; Developmental

Genotype

Glucosamine/chemistry

Glucuronic Acid/metabolism

Glycosaminoglycans

Heparitin Sulfate/*biosynthesis

Iduronic Acid/metabolism

Mice

Mice; Transgenic

Nitrous Acid/metabolism

Polysaccharides/chemistry

Research Support; Non-U.S. Gov't

Reverse Transcriptase Polymerase Chain Reaction

Stem Cells/metabolism

Sulfates/chemistry

Sulfotransferases/*genetics/physiology

Sulfur/*metabolism

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)