Portable electroanalytical nucleic acid amplification tests using printed circuit boards and open-source electronics

• The realization of electrochemical nucleic acid amplification tests (NAATs) at the point of care (POC) is highly desirable, but it remains a challenge given their high cost and lack of true portability/miniaturization. Here we show that mass-produced, industrial standardized, printed circuit boards (PCBs) can be repurposed to act as near-zero cost electrodes for self-assembled monolayer-based DNA biosensing, and further integration with a custom-designed and low-cost portable potentiostat. To show the analytical capability of this system, we developed a NAAT using isothermal recombinase polymerase amplification, bypassing the need of thermal cyclers, followed by an electrochemical readout relying on a sandwich hybridization assay. We used our sensor and device for analytical detection of the toxic microalgae Ostreopsis cf. ovata as a proof of concept. This work shows the potential of PCBs and open-source electronics to be used as powerful POC DNA biosensors at a low-cost. 

Ämnesord

NATURVETENSKAP  -- Kemi -- Analytisk kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences -- Analytical Chemistry (hsv//eng)

Nyckelord

Biosensing Techniques

DNA

Electronics

Nucleic Acid Amplification Techniques

Recombinases

Costs

Nucleic acids

Self assembled monolayers

Timing circuits

Voltage regulators

recombinase

DNA biosensing

Electrochemicals

High costs

Low-costs

Miniaturisation

Nucleic acids amplification

Open-source

Point of care

Potentiostats

genetic procedures

genetics

Printed circuit boards

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)