| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Stritzinger, M. D., et al.
(författare)
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The Carnegie Supernova Project II : Observations of the luminous red nova AT 2014ej
- 2020
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Ingår i: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 639
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Tidskriftsartikel (refereegranskat)abstract
- We present optical and near-infrared broadband photometry and optical spectra of AT 2014ej from the Carnegie Supernova Project-II. These observations are complemented with data from the CHilean Automatic Supernova sEarch, the Public ESO Spectroscopic Survey of Transient Objects, and from the Backyard Observatory Supernova Search. Observational signatures of AT 2014ej reveal that it is similar to other members of the gap-transient subclass known as luminous red novae (LRNe), including the ubiquitous double-hump light curve and spectral properties similar to that of LRN SN 2017jfs. A medium-dispersion visual-wavelength spectrum of AT 2014ej taken with the Magellan Clay telescope exhibits a P Cygni H alpha feature characterized by a blue velocity at zero intensity of approximate to 110 km s(-1) and a P Cygni minimum velocity of approximate to 70 km s(-1). We attribute this to emission from a circumstellar wind. Inspection of pre-outbust Hubble Space Telescope images yields no conclusive progenitor detection. In comparison with a sample of LRNe from the literature, AT 2014ej lies at the brighter end of the luminosity distribution. Comparison of the ultra-violet, optical, infrared light curves of well-observed LRNe to common-envelope evolution models from the literature indicates that the models underpredict the luminosity of the comparison sample at all phases and also produce inconsistent timescales of the secondary peak. Future efforts to model LRNe should expand upon the current parameter space we explore here and therefore may consider more massive systems and a wider range of dynamical timescales.
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