| 1. |
- SCHUBERT, F, et al.
(författare)
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ORGANIC-PHASE ENZYME ELECTRODES FOR THE DETERMINATION OF HYDROGEN-PEROXIDE AND PHENOL
- 1992
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Ingår i: Sensors and actuators. B, Chemical. - : Elsevier Science B.V., Amsterdam.. - 0925-4005 .- 1873-3077. ; 7:03-jan, s. 408-411
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Tidskriftsartikel (refereegranskat)abstract
- An amperometric horseradish peroxidase electrode is described for the determination of hydrogen peroxide in organic solvents. The enzyme was co-adsorbed with an electron mediator, hexacyanoferrate(II), to the surface of a graphite foil electrode making reagentless measurement possible. The electrochemical reduction of the enzymatically oxidized mediator was utilized as the analytical signal. The electrode can be operated in dioxane, chloroform and chlorobenzene, the presence of a small quantity of aqueous buffer being essential for activity. On this basis a small, probe-type sensor has been assembled the response of which is linearly related to hydrogen peroxide concentration between 0.05 and 1 mM. A tyrosinase sensor has been constructed by combining a Clark-type oxygen electrode with a membrane bearing adsorbed enzyme. The sensor is capable of measuring between 0.1 and 5 mM phenol in chloroform saturated with aqueous buffer.
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| 2. |
- Alcock, S. J., et al.
(författare)
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Advances in the use of in vivo sensors
- 1992
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Ingår i: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 7:4, s. 243-254
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The sixth workshop of the European Community Concerted Action on Chemical sensors for in vivo monitoring was held at Snogeholm, Sweden, in October 1991. The meeting reviewed recent in vivo and ex vivo results, and also included a shorter session on ethical and safety problems.
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| 3. |
- SCHUBERT, F, et al.
(författare)
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MEDIATED AMPEROMETRIC ENZYME ELECTRODE INCORPORATING PEROXIDASE FOR THE DETERMINATION OF HYDROGEN-PEROXIDE IN ORGANIC-SOLVENTS
- 1991
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Ingår i: Analytica Chimica Acta. - : Elsevier Science B.V., Amsterdam.. - 0003-2670 .- 1873-4324. ; 245:2, s. 133-138
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Tidskriftsartikel (refereegranskat)abstract
- An amperometric enzyme electrode incorporating horseradish peroxidase is described for the determination of hydrogen peroxide in organic solvents. The enzyme was co-adsorbed with an electron mediator, potassium hexacyanoferrate(II), on the surface of a graphite foil electrode, making reagentless measurement possible. The electrochemical reduction of the enzymatically oxidized mediator was utilized as the analytical signal. Studies in different solvent systems revealed that the electrode could be operated in dioxane, chloroform and chlorobenzene, the last two providing approximately double the sensitivity of the former. The presence of a small amount of aqueous buffer was essential for sensor activity. During 2 weeks of intermittent use, the sensitivity of the electrode decreased to 40% of its initial value. At least 50 assays could be performed with a single sensor.
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