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- Danielsson, B., et al.
(författare)
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Thermistor-based biosensing
- 1997
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Ingår i: Frontiers in Biosensorics II. - 1023-294X. - 9783764354794 - 9783034890458 ; 81, s. 71-85
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Bokkapitel (refereegranskat)abstract
- In this review a universal thermistor-based biosensor system is described with examples from clinical chemistry, bioprocess monitoring and environmental control. The technique is based on the measurement of the small temperature changes associated with enzymatic reactions occurring in a microreactor with immobilized enzyme. The system has good operational stability and a sensitivity that permits measurements down to 1 μM concentrations. Current developments include devices constructed by micromachining for multisensing purposes and miniaturised instrumentation intended for use in portable monitoring. With use of special supports for enzyme immobilisation even untreated whole blood samples can be applied. Another current line of investigation involves hybrid biosensors, such as combinations of electrochemistry and calorimetry into bioelectrocalorimetric devices with interesting new properties.
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| 4. |
- Skytting, B, et al.
(författare)
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A novel fusion gene, SYT-SSX4, in synovial sarcoma
- 1999
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Ingår i: Journal of the National Cancer Institute. - : Oxford University Press (OUP). - 0027-8874 .- 1460-2105. ; 91:11, s. 974-975
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 7. |
- Werr, J, et al.
(författare)
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beta1 integrins are critically involved in neutrophil locomotion in extravascular tissue In vivo
- 1998
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Ingår i: The Journal of experimental medicine. - : Rockefeller University Press. - 0022-1007 .- 1540-9538. ; 187:12, s. 2091-2096
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Tidskriftsartikel (refereegranskat)abstract
- Recruitment of leukocytes from blood to tissue in inflammation requires the function of specific cell surface adhesion molecules. The objective of this study was to identify adhesion molecules that are involved in polymorphonuclear leukocyte (PMN) locomotion in extravascular tissue in vivo. Extravasation and interstitial tissue migration of PMNs was induced in the rat mesentery by chemotactic stimulation with platelet-activating factor (PAF; 10−7 M). Intravital time-lapse videomicroscopy was used to analyze migration velocity of the activated PMNs, and the modulatory influence on locomotion of locally administered antibodies or peptides recognizing various integrin molecules was examined. Immunofluorescence flow cytometry revealed increased expression of α4, β1, and β2 integrins on extravasated PMNs compared with blood PMNs. Median migration velocity in response to PAF stimulation was 15.5 ± 4.5 μm/min (mean ± SD). Marked reduction (67 ± 7%) in motility was observed after treatment with mAb blocking β1 integrin function (VLA integrins), whereas there was little, although significant, reduction (22 ± 13%) with β2 integrin mAb. Antibodies or integrin-binding peptides recognizing α4β1, α5β1, or αvβ3 were ineffective in modulating migration velocity.Our data demonstrate that cell surface expression of β1 integrins, although limited on blood PMNs, is induced in extravasated PMNs, and that members of the β1 integrin family other than α4β1 and α5β1 are critically involved in the chemokinetic movement of PMNs in rat extravascular tissue in vivo.
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| 12. |
- Yuan, XiMing, et al.
(författare)
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The establishment of two cell lines from a mouse uterine cervical carcinoma (U14) and their metastatic phenotype changes
- 1995
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Ingår i: Clinical and Experimental Metastasis. - 0262-0898 .- 1573-7276. ; 13:6, s. 463-73
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Tidskriftsartikel (refereegranskat)abstract
- This paper studies the heterogeneity of metastatic potential of murine cervical carcinoma (U14). Two cell lines, P11-90 and L10-90, were established from a pulmonary metastatic substrain (U14AP11) and a lymphatic metastatic substrain (U14AL10), which were selected from U14 in vivo after 11 and 10 passages, respectively. The biologic differences between the two cell lines are as follows. (1) The cells of the P11-90 line grow more rapidly compared with the L10-90 line. From the 40th passage the medium pH was different. (2) The median number of chromosomes in P11-90 and L10-90 was 72 and 64, respectively; the rates of gap aberration were 88% and 78%, respectively. (3) The number of T lymphocytes and T helper lymphocytes in the peripheral blood from hosts with P11-90 were higher than that of hosts transplanted with L10-90, but the number of B lymphocytes in the latter was larger than that in the former. (4) The metastatic potential of each cell line partially decreased compared to the relative tumor substrain, but their organ preference still remained and the transplant locations, axillary or footpad, had a prominent influence on their metastatic behavior. To observe the effects of metastatic target organs on the metastatic phenotypes of tumor cells, as well as to explore a method for the establishment and maintenance of the metastatic organ preference of tumor cells, conditioned medium (CM) from pulmonary or lymphatic node diploid cells was added to the culture medium of P11-90 and L10-90. Two sublines, P + P11-90 and Ln + L10-90, were thus established. Using stereological methods we found that the majority of P + P11-90 cells became larger and their nuclei also increased in size compared with their parental lines, but the majority of Ln + L10-90 cells became smaller in size, though the nuclei were enlarged. The pulmonary metastatic rate and lymphatic metastatic rate of P + P11-90, as well as the lymphatic metastatic rate of Ln + L10-90, were restored dramatically. The results suggest that by taking advantage of the interaction between tumor cells and the CM of host cells the metastatic potential of tumor cell lines can be maintained in vitro. Our work may offer an experimental model for the manipulation of metastasis of cell lines coming from the same parent strain but with different metastatic potentials.(ABSTRACT TRUNCATED AT 400 WORDS)
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