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Intestinal CD103+CD...
Intestinal CD103+CD11b+ cDC2 conventional dendritic cells are required for primary CD4+ T and B cell responses to soluble flagellin
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- Flores-Langarica, Adriana (author)
- University of Birmingham
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- Cook, Charlotte (author)
- University of Birmingham
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- Luda, Katarzyna Müller (author)
- Lund University,Lunds universitet,Slemhinnans immunologi,Forskargrupper vid Lunds universitet,Mucosal Immunology,Lund University Research Groups
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- Persson, Emma K. (author)
- Ghent University
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- Marshall, Jennifer L. (author)
- University of Birmingham
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- Beristain-Covarrubias, Nonantzin (author)
- University of Birmingham
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- Yam-Puc, Juan Carlos (author)
- University of Birmingham
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- Dahlgren, Madelene (author)
- Lund University,Lunds universitet,Adaptivt immunförsvar,Forskargrupper vid Lunds universitet,Adaptive Immunity,Lund University Research Groups
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- Persson, Jenny J. (author)
- Lund University,Lunds universitet,Värd-patogeninteraktioner,Forskargrupper vid Lunds universitet,Host-Pathogen Interactions,Lund University Research Groups
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- Uematsu, Satoshi (author)
- University of Tokyo,Osaka City University
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- Akira, Shizuo (author)
- Osaka University
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- Henderson, Ian R. (author)
- University of Birmingham
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- Lindbom, Bengt Johansson (author)
- Lund University,Lunds universitet,Adaptivt immunförsvar,Forskargrupper vid Lunds universitet,Adaptive Immunity,Lund University Research Groups,Technical University of Denmark
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- Agace, William (author)
- Lund University,Lunds universitet,Slemhinnans immunologi,Forskargrupper vid Lunds universitet,Mucosal Immunology,Lund University Research Groups,Technical University of Denmark
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- Cunningham, Adam F. (author)
- University of Birmingham
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(creator_code:org_t)
- 2018-10-17
- 2018
- English.
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In: Frontiers in Immunology. - : Frontiers Media SA. - 1664-3224. ; 9:OCT
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Abstract
Subject headings
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- Systemic immunization with soluble flagellin (sFliC) from Salmonella Typhimurium induces mucosal responses, offering potential as an adjuvant platform for vaccines. Moreover, this engagement of mucosal immunity is necessary for optimal systemic immunity, demonstrating an interaction between these two semi-autonomous immune systems. Although TLR5 and CD103+CD11b+ cDC2 contribute to this process, the relationship between these is unclear in the early activation of CD4+ T cells and the development of antigen-specific B cell responses. In this work, we use TLR5-deficient mice and CD11c-cre.Irf4fl/fl mice (which have reduced numbers of cDC2, particularly intestinal CD103+CD11b+ cDCs), to address these points by studying the responses concurrently in the spleen and the mesenteric lymph nodes (MLN). We show that CD103+CD11b+ cDC2 respond rapidly and accumulate in the MLN after immunization with sFliC in a TLR5-dependent manner. Furthermore, we identify that whilst CD103+CD11b+ cDC2 are essential for the induction of primary T and B cell responses in the mucosa, they do not play such a central role for the induction of these responses in the spleen. Additionally, we show the involvement of CD103+CD11b+ cDC2 in the induction of Th2-associated responses. CD11c-cre.Irf4fl/fl mice showed a reduced primary FliC-specific Th2-associated IgG1 responses, but enhanced Th1-associated IgG2c responses. These data expand our current understanding of the mucosal immune responses promoted by sFliC and highlights the potential of this adjuvant for vaccine usage by taking advantage of the functionality of mucosal CD103+CD11b+ cDC2.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- CDC2
- Dendritic cells
- Flagellin
- Immune response
- Mucosa
Publication and Content Type
- art (subject category)
- ref (subject category)
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Flores-Langarica ...
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Cook, Charlotte
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Luda, Katarzyna ...
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Persson, Emma K.
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Marshall, Jennif ...
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Beristain-Covarr ...
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Yam-Puc, Juan Ca ...
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Dahlgren, Madele ...
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Persson, Jenny J ...
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Uematsu, Satoshi
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Akira, Shizuo
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Henderson, Ian R ...
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Lindbom, Bengt J ...
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Agace, William
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Cunningham, Adam ...
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