| 1. |
- Belew, M., et al.
(författare)
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Purification of Recombinant Human Serum Albumin (rHSA) Produced by Genetically Modified Pichia Pastoris
- 2008
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Ingår i: Separation science and technology (Print). - : Informa UK Limited. - 0149-6395 .- 1520-5754. ; 43:11-12, s. 3134-3153
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Tidskriftsartikel (refereegranskat)abstract
- Recombinant human serum albumin (rHSA) was produced by genetically transformed Pichia pastoris yeast. The cell-culture supernatant (CCS) contained 8–12 g/l rHSA that was purified in a three-step procedureinvolving (1) a capture step using the newly developed cation exchanger CaptoTM MMC; (2) an intermediate step using Phenyl SepharoseTM and, (3) a polishingstep using Aminobutyl SepharoseTM 6 FF. The total recovery was 25–35% and the product fulfils the purity criteria of the European Pharmacopeia. Purified rHSA and plasma-derived HSA were essentially identical judging bySDS- or native-PAGE, and the pigment level (expressed as A 350/A280) in the rHSA was 0.03 or less and was strongly dependent on the quality of the CCS.Dimers and polymers in the final product were less than that found in purified plasma-derived HSA. The molar mass of the purified rHSA, as well as of its natural counterpart, is 67 000 Daltons by MALDI-ToF mass spectrometry, while the iso-electric points of both recombinant and natural HSA ranged between pH 5.42–5.55 when determined in 8M urea. The stability profiles of both proteins after heat treatment were identical as determined by differential scanning calorimetry (DSC). The results obtained here suggest the purified rHSA to be a homogeneous protein identical to its natural counterpart.
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| 2. |
- Gao, Yan, et al.
(författare)
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Synthesis and characterization of manganese and copper corrole xanthene complexes as catalysts for water oxidation
- 2007
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Ingår i: Tetrahedron. - : Elsevier BV. - 0040-4020 .- 1464-5416. ; 63:9, s. 1987-1994
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Tidskriftsartikel (refereegranskat)abstract
- Two corrole xanthene ligands and four corresponding Mn-IV and Cu-III complexes have been synthesized and spectroscopically characterized. This kind of complexes, comprising of xanthene and corrole linked by an amide bond, were designed as bio-inspired models for the oxygen evolving complex (OEC) in Photosystem II. We find that both manganese complexes 4a and 5a have efficiency on catalyzing oxygen evolution at low potential (about 0.80 V) by electrochemical method, which is a significant progress in the study of dioxygen formation.
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| 3. |
- Liu, Kui, et al.
(författare)
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Kallikrein genes are associated with lupus and glomerular basement membrane-specific antibody-induced nephritis in mice and humans
- 2009
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Ingår i: Journal of Clinical Investigation. - 0021-9738 .- 1558-8238. ; 119:4, s. 911-923
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Tidskriftsartikel (refereegranskat)abstract
- Immune-mediated nephritis contributes to disease in systemic lupus erythematosus, Goodpasture syndrome (caused by antibodies specific for glomerular basement membrane [anti-GBM antibodies]), and spontaneous lupus nephritis. Inbred mouse strains differ in susceptibility to anti-GBM antibody-induced and spontaneous lupus nephritis. This study sought to clarify the genetic and molecular factors that maybe responsible for enhanced immune-mediated renal disease in these models. When the kidneys of 3 mouse strains sensitive to anti-GBM antibody-induced nephritis were compared with those of 2 control strains using microarray analysis, one-fifth of the underexpressed genes belonged to the kallikrein gene family,which encodes serine esterases. Mouse strains that upregulated renal and urinary kallikreins exhibited less evidence of disease. Antagonizing the kallikrein pathway augmented disease, while agonists dampened the severity of anti-GBM antibody-induced nephritis. In addition, nephritis-sensitive mouse strains had kallikrein haplotypes that were distinct from those of control strains, including several regulatory polymorphisms,some of which were associated with functional consequences. Indeed, increased susceptibility to anti-GBM antibody-induced nephritis and spontaneous lupus nephritis was achieved by breeding mice with a genetic interval harboring the kallikrein genes onto a disease-resistant background. Finally, both human SLE and spontaneous lupus nephritis were found to be associated with kallikrein genes, particularly KLK1 and the KLK3 promoter, when DNA SNPs from independent cohorts of SLE patients and controls were compared. Collectively, these studies suggest that kallikreins are protective disease-associated genes in anti-GBM antibody-induced nephritis and lupus.
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| 4. |
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| 5. |
- Privalov, Timofei, et al.
(författare)
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A computational study of O-O bond formation catalyzed by monoand Bis-Mn-IV-Corrole complexes
- 2007
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Ingår i: Inorganic Chemistry. - : American Chemical Society (ACS). - 0020-1669 .- 1520-510X. ; 46:17, s. 7075-7086
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Tidskriftsartikel (refereegranskat)abstract
- A detailed computational study of O-O bond formation, catalyzed by monomeric and dimeric Mn-corrole complexes, is reported. The model explicitly takes into account the solvent, with respect to the first and second coordination spheres, while the bulk solvent is described by the polarizable continuum model. Two reaction mechanisms are proposed and computationally characterized: the concerted and the two-step mechanisms. The concerted mechanism is based on a OH--(MnO)-O-IV interaction via the outer-sphere pathway involving the bridging solvent molecules in the first coordinating sphere. The two-step mechanism is proposed to operate via the coordination of a hydroxide to the Mn-Iv ion, forming a MnO(OH)(-)-corrole complex with a strongly nonplanar corrole ligand. Comparison of the proposed mechanisms with available experimental data. is performed.
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| 6. |
- Tatarek-Nossol, Marianna, et al.
(författare)
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Inhibition of hIAPP amyloid-fibril formation and apoptotic cell death by a designed hIAPP amyloid-core-containing hexapeptide
- 2005
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Ingår i: Chemistry and Biology. - : Elsevier BV. - 1074-5521 .- 1879-1301. ; 12:7, s. 797-809
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Tidskriftsartikel (refereegranskat)abstract
- The pathogenesis of type II diabetes is associated with the aggregation of the 37-residue human islet amyloid polypeptide (hIAPP) into cytotoxic β sheet aggregates and fibrils. We have recently shown that introduction of two N-methyl rests in the β sheet- and amyloid-core-containing sequence hIAPP(22-27), or NFGAIL converted this amyloidogenic and cytotoxic sequence into nonamyloidogenic and noncytotoxic NF(N-Me)GA(N-Me)IL. Here, we show that NF(N-Me)GA(N-Me)IL is able to bind with high-affinity full-length hIAPP and to inhibit its fibrillogenesis. NF(N-Me)GA(N-Me)IL also inhibits hIAPP-mediated apoptotic β cell death. By contrast, unmodified NFGAIL does not inhibit hIAPP amyloidogenesis and cytotoxicity, suggesting that N-methylation conferred on NFGAIL the properties of NF(N-Me)GA(N-Me)IL. These results support the concept that rational N-methylation of hIAPP amyloid-core sequences may be a valuable strategy to design pancreatic-amyloid diagnostics and therapeutics for type II diabetes. ©2005 Elsevier Ltd All rights reserved.
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| 7. |
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