| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Cai, Xiao, et al.
(författare)
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Propagation of Darrieus-Landau unstable laminar and turbulent expanding flames
- 2021
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Ingår i: Proceedings of the Combustion Institute. - : Elsevier BV. - 1540-7489. ; 38:2, s. 2013-2021
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Tidskriftsartikel (refereegranskat)abstract
- The propagation of laminar and turbulent expanding flames subjected to Darrieus-Landau (DL), hydrodynamic instability was experimentally studied by employing stoichiometric H2/O2/N2 flames under quiescent and turbulent conditions performed in a newly developed medium-scale, fan-stirred combustion chamber. In quiescent environment, DL unstable laminar flame exhibits three-stage propagation, i.e. smooth expansion, transition acceleration, and self-similar acceleration. The self-similar acceleration is characterized by a power-law growth of acceleration exponent, α, with normalized Peclet number, which is different from the usually suggested self-similar propagation with a constant α. The imposed turbulence advances the onset of both transition acceleration and self-similar acceleration stages and promotes the strength of flame acceleration as additional wrinkles are invoked by turbulence eddies. A DL-turbulent interaction regime is confirmed to be the classical corrugated flamelets regime. Furthermore, the DL instability significantly facilitates the propagation of expanding flames in medium and even intense turbulence. The development of DL cells is not suppressed by turbulence eddies, and it needs to be considered in turbulent combustion.
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| 3. |
- Chen, Haoran, et al.
(författare)
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Decoupling engineering of formamidinium-cesium perovskites for efficient photovoltaics
- 2022
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Ingår i: National Science Review. - : Oxford University Press. - 2095-5138 .- 2053-714X. ; 9:10
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Tidskriftsartikel (refereegranskat)abstract
- Sequential Cs incorporation strategy is developed to decouple crystallization of FACs perovskite with reduced electron-phonon coupling, resulting in highly stable FACs tri-iodide perovskite photovoltaics with record efficiency. Although pure formamidinium iodide perovskite (FAPbI(3)) possesses an optimal gap for photovoltaics, their poor phase stability limits the long-term operational stability of the devices. A promising approach to enhance their phase stability is to incorporate cesium into FAPbI(3). However, state-of-the-art formamidinium-cesium (FA-Cs) iodide perovskites demonstrate much worse efficiency compared with FAPbI(3), limited by the different crystallization dynamics of formamidinium and cesium, which result in poor composition homogeneity and high trap densities. We develop a novel strategy of crystallization decoupling processes of formamidinium and cesium via a sequential cesium incorporation approach. As such, we obtain highly reproducible, highly efficient and stable solar cells based on FA(1)(-)(x)Cs(x)PbI(3) (x = 0.05-0.16) films with uniform composition distribution in the nanoscale and low defect densities. We also revealed a new stabilization mechanism for Cs doping to stabilize FAPbI(3), i.e. the incorporation of Cs into FAPbI(3) significantly reduces the electron-phonon coupling strength to suppress ionic migration, thereby improving the stability of FA-Cs-based devices.
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| 4. |
- Jiang, X., et al.
(författare)
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Rural tourism network evaluation based on resource control ability analysis : A case study of Ning’an, China
- 2021
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Ingår i: Land. - : MDPI AG. - 2073-445X. ; 10:4
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Tidskriftsartikel (refereegranskat)abstract
- Organization of rural tourism resources is important for optimizing rural land use based on rational resource classification. Quantitative analysis was performed to evaluate the resource control ability of rural tourism networks. This was achieved by determining the resource control relationship and assessing the structure of the rural tourism network. The ability of resource control was analyzed via resource abstraction, which included the extraction of resource nodes and corridors, control scope analysis, and network structure level evaluation. The proposed approach was applied to the Ning’an in Heilongjiang Province, China, and proved to be effective for exploring the network degree and development trends in rural tourism resources. By examining the resource control ability, the spatial characteristics and development trend in rural tourism networks were quantitatively analyzed, especially the connection mode of key tourism resources, network structure analysis, and resource linking ability. The core resources showed a lack of outward ability in the network, and the secondary resource expansion ability was limited. Via resource control ability analysis, this study focused on areas with rich tourism but an unbalanced spatial structure, combining the directional characteristics of the network to provide suggestions for the optimization rural tourism resources network in other regions of the world.
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| 5. |
- Ju, Haoran, et al.
(författare)
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LES and URANS study on turbulent flow through 3 x 3 rod bundle with spacer grid and mixing vanes using spectral element method
- 2021
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Ingår i: Annals of Nuclear Energy. - : Elsevier BV. - 0306-4549 .- 1873-2100. ; 161, s. 108474-
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Tidskriftsartikel (refereegranskat)abstract
- In this paper, the turbulent flow in rod bundles with spacer grid and mixing vanes in nuclear reactor core was studied using spectral element method with large eddy simulation (LES) and unsteady RANS (URANS) method. Combining with the radial periodical boundary conditions, the turbulent mixing phenomena in central subchannels were investigated, especially focusing on the flow field after the spacer grid and mixing vanes. Other than setting up long bare rod bundle to establish the fully developed turbulent flow, only a 2-cm-long bare rod bundle was extruded upstream the spacer grid and mixing vanes, while the fully developed laminar velocity profile was applied on the inlet surface for velocity boundary settings. The results indicate that both of the models could predict time-averaged velocity at 0.5Dh, 1Dh, 4Dh downstream mixing vanes properly, while the URANS model underestimate the root-mean-square (RMS) value of fluctuating velocity due to the inlet treatment effect. Furthermore, turbulent kinetic energy contour with transverse velocity vector was combined to investigate the behaviors of vortices formed by split-type mixing vanes, revealing the behavior of vortices for preliminary dynamic and mechanic analysis in design process of fuel assembly.
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| 6. |
- Li, Shicong, et al.
(författare)
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The Tribological Performance of Frictional Pair of Gas–Liquid Miscible Backflow Pumping Seal in Oil–Air Environment
- 2023
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Ingår i: Lubricants. - : MDPI. - 2075-4442. ; 11:5
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Tidskriftsartikel (refereegranskat)abstract
- The gas–liquid miscible backflow pumping seal (G-LMBPHS) is a non-contact mechanical seal that is suitable for high-speed bearing chambers. However, the tribological properties and wear mechanisms of the frictional pair of G-LMBPHS in an oil–air environment have not yet been comprehensively studied. In this study, the tribological properties of six frictional pairs, consisting of three hard materials (18Cr2Ni4WA, Al2O3 coating, and Cr2O3 coating) and two soft materials (metal-impregnated graphite [Metal-IG] and resin-impregnated graphite [Resin-IG]), were analyzed using a disc-on-disc tribometer. An oil–air environment was created using a minimal quantity lubrication (MQL) system and a closed chamber. The results show that the COF of the four frictional pairs consisting of two coatings and two graphites decreases gradually with increasing rotational speed, and the frictional pairs composed of Al2O3 coating and Resin-IG and Cr2O3 coating and Resin-IG have the lowest COF between 0.022 and 0.03. Therefore, the frictional pairs of G-LMBPHS are in a mixed lubrication condition. The lubricant in the oil–air environment is adsorbed and stored in pits on the surface of graphite and coatings, enhancing the hydrodynamic effect of the spiral grooves and reducing the COF by up to 45%. Metal-IG has better wear resistance than Resin-IG, and the frictional pair consisting of Cr2O3 coating and Metal-IG has the lightest wear. This study provides an important basis for the selection of G-LMBPHS frictional pairs in oil–air environments.
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| 7. |
- Wang, Xiaoyu, et al.
(författare)
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Guest Editorial: Modeling and Simulation Methods for Analysis and Design of Advanced Energy Conversion Systems
- 2020
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Ingår i: IEEE Transactions on Energy Conversion. - 1558-0059 .- 0885-8969. ; 35:1, s. 309-311
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The papers in this special section examine modeling and simulation methods for the analysis and design of advanced energy conversion systems. Energy conversion lies aEnergyveral fronts including materials, conversion methods, power electronics, and controls have created new opportunities for efficient energy conversion from both the conventional and new sources of energy. Our urgent need to solve many critical problems with regards to the sustainability and security of our energy system as well as the ever-increasing environmental challenges facing humanity have further spurred unprecedented opportunities for creation of innovative solutions to address these challenges.
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| 8. |
- Wu, Chuansha, et al.
(författare)
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Modification of Food Allergy on the Associations between Early Life Exposure to Size-Specific Particulate Matter and Childhood Allergic Rhinitis
- 2024
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Ingår i: Environmental Science and Technology. - : American Chemical Society (ACS). - 0013-936X .- 1520-5851. ; 58:4, s. 1813-1822
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Tidskriftsartikel (refereegranskat)abstract
- Previous studies have reported the association between particulate matter (PM) and childhood allergic rhinitis (AR). However, it is unclear whether food allergy (FA) modifies the PM-AR association. We aimed at evaluating the effect of the modification of FA on PM-AR association in preschool children. We adopted a cross-sectional study and conducted a questionnaire survey among preschool children aged 3-6 years in 7 cities in China from June 2019 to June 2020 to collect information on AR and FA. We used a combination of multilevel logistic regression and restricted cubic spline functions to quantitatively assess whether FA modifies the associations between size-specific PM exposure (1 x 1 km) and the risk of AR. The adjusted odds ratios (ORs) for AR among the children with FA as per a 10 mu g/m(3) increase in early life PM1, PM2.5, and PM10 were significantly higher than the corresponding ORs among the children without FA [e.g., OR: 1.58, 95% CI: (1.32, 1.90) vs 1.29, 95% CI: (1.18, 1.41), per 10 mu g/m(3) increase in PM1]. The interactions between FA and size-specific PM exposure and their effects on AR were statistically significant (all p-int < 0.001). FA, as an important part of the allergic disease progression, may modify the PM-AR association in preschool children.
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| 9. |
- Zhao, Jinli, et al.
(författare)
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Cloud-Edge Collaboration-Based Local Voltage Control for DGs With Privacy Preservation
- 2023
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Ingår i: IEEE Transactions on Industrial Informatics. - : IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC. - 1551-3203 .- 1941-0050. ; 19:1, s. 98-108
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Tidskriftsartikel (refereegranskat)abstract
- The increased distributed generators (DGs) have exacerbated voltage violations in active distribution networks (ADNs). Local reactive power control of DG inverters can realize a fast response to frequent voltage fluctuations. However, commonly used model-based voltage control depends upon accurate network parameters and entire ADN data, which may cause the sensitive information leakage of ADN and DG behaviors in practical operation. In this article, a cloud-edge collaboration-based local voltage control strategy for DGs is proposed with privacy preservation. First, a local voltage control framework is established based on cloud-edge collaboration, in which a surrogate model is built based on the graph convolutional neural networks to estimate the ADN voltages. By transferring the surrogate model, the edge side can obtain the exact voltage estimation in the local curve tuning process without the authority of the whole ADN data, preserving the network parameters of ADN. Then, the interarea coordination based on federated learning is proposed to realize the parameter updating of DG control curves, which can achieve better voltage control performance. By updating surrogate submodels based on private data distributed across multiple edge devices, federated learning can effectively preserve DG behaviors. Finally, the effectiveness and adaptability of the proposed control strategy are validated using the modified IEEE 33-node system. The proposed local DG control strategy can effectively cope with voltage problems and enhance the adaptability to variations in practical operation states while considering privacy preservation.
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| 10. |
- Zhao, Jinli, et al.
(författare)
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Peer-to-Peer electricity trading of interconnected flexible distribution networks based on Non-Cooperative games
- 2023
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Ingår i: International Journal of Electrical Power & Energy Systems. - : Elsevier Ltd. - 0142-0615 .- 1879-3517. ; 145
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Tidskriftsartikel (refereegranskat)abstract
- With the integration of power electronic devices represented by soft open points (SOPs), distribution networks have gradually evolved into interconnected flexible distribution networks (FDNs). Considering the deregulation of electricity market and user privacy, multiple stakeholders have participated in the operation of FDNs. Peer-to-peer (P2P) electricity trading is promising to alleviate operational problems of interconnected FDNs. As multiple regions pursue the maximum profits individually, non-cooperative game methods can be utilized to realize fair profit allocation in P2P trading. In this paper, a non-cooperative game-based P2P trading method is proposed to meet the electricity trading needs of multi-region interconnected FDNs. First, based on non-cooperative games, a two-layer P2P electricity trading framework is established to realize cost reduction and voltage profile improvement of multi-region interconnected FDNs. Then, a P2P trading adjustment mechanism is designed to improve the operational profits of SOP, in which spatial active power trading adjustment, temporal dispatching of energy storage (ES) link and reactive power support are incorporated. Finally, the effectiveness of the proposed method is verified based on a practical distribution network with four-terminal SOP in Tianjin. The results show that the proposed P2P electricity trading method can promote the economic operation performance of interconnected FDNs and improve the operational profit of SOP.
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| 11. |
- Zhao, Ning, et al.
(författare)
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Emerging information and communication technologies for smart energy systems and renewable transition
- 2023
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Ingår i: Advances in Applied Energy. - : Elsevier Ltd. - 2666-7924. ; 9
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Tidskriftsartikel (refereegranskat)abstract
- Since the energy sector is the dominant contributor to global greenhouse gas emissions, the decarbonization of energy systems is crucial for climate change mitigation. Two major challenges of energy systems decarbonization are renewable transition planning and sustainable systems operations. To address the challenges, incorporating emerging information and communication technologies can facilitate both the design and operations of future smart energy systems with high penetrations of renewable energy and decentralized structures. The present work provides a comprehensive overview of the applicability of emerging information and communication technologies in renewable transition and smart energy systems, including artificial intelligence, quantum computing, blockchain, next-generation communication technologies, and the metaverse. Relevant research directions are introduced through reviewing existing literature. This review concludes with a discussion of the industrial use cases and demonstrations of smart energy technologies.
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| 12. |
- Zhao, Shengzhong, et al.
(författare)
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Theoretical and numerical study on smoke descent during tunnel fires under natural ventilation condition
- 2023
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Ingår i: Tunnelling and Underground Space Technology. - : Elsevier Ltd. - 0886-7798 .- 1878-4364. ; 142
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Tidskriftsartikel (refereegranskat)abstract
- The smoke stratification and the smoke descent along a tunnel are of the utmost importance for personnel evacuation. The paper investigates the smoke descent along a tunnel during a naturally ventilated tunnel fire. A theoretical model is developed to predict the smoke depth below the ceiling along the tunnel. A series of numerical simulations of full-scale tunnel fires are conducted to compare with the developed model, and some coefficients such as the entrainment coefficient are determined from the simulation results. The concepts of critical moment and critical distance are proposed to characterize the smoke descent along the tunnel. The results show that as the smoke spreads longitudinally, the smoke depth below the tunnel ceiling continuously increases. The temperature decay along the tunnel due to heat losses and air entrainment at the smoke layer interface is considered as the main parameter for the smoke descent. After the vitiated air returns back to the fire source, the smoke stratification in the entire tunnel will be significantly reduced. The smoke layer depth along the tunnel based on the temperature distribution is relatively stable in the process of smoke development, which is not sensitive to the HRR, but influenced by the tunnel width, and this method could only be used before the critical moment. The outcomes of this study could provide references for a better understanding of smoke movement in naturally ventilated tunnels and provide technical guidelines for fire safety designers.
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