| 51. |
- Park, Y I, et al.
(författare)
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Role of a novel photosystem II-associated carbonic anhydrase in photosynthetic carbon assimilation in Chlamydomonas reinhardtii
- 1999
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Ingår i: FEBS Letters. - 0014-5793 .- 1873-3468. ; 444:1, s. 102-105
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Tidskriftsartikel (refereegranskat)abstract
- Intracellular carbonic anhydrases (CA) in aquatic photosynthetic organisms are involved in the CO2-concentrating mechanism (CCM), which helps to overcome CO2 limitation in the environment. In the green alga Chlamydomonas reinhardtii, this CCM is initiated and maintained by the pH gradient created across the chloroplast thylakoid membranes by photosystem (PS) II-mediated electron transport. We show here that photosynthesis is stimulated by a novel, intracellular alpha-CA bound to the chloroplast thylakoids. It is associated with PSII on the lumenal side of the thylakoid membranes. We demonstrate that PSII in association with this lumenal CA operates to provide an ample flux of CO2 for carboxylation. (C) 1999 Federation of European Biochemical Societies.
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| 52. |
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| 53. |
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| 54. |
- Williams, W P, et al.
(författare)
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X-ray diffraction studies of the structural organisation of prolamellar bodies isolated from Zea mays
- 1998
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Ingår i: FEBS Letters. - 0014-5793 .- 1873-3468. ; 422:2, s. 252-254
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Tidskriftsartikel (refereegranskat)abstract
- Transmission electron microscopy (TEM) indicates that maize prolamellar bodies (PLBs) are built up of tetrapodal units based on a highly convoluted but continuous lipid bilayer exhibiting diamond cubic (Fd3m) symmetry. Such lattices are often described in terms of infinite periodic minimal surfaces (IMPS) exhibiting zero net curvature and dividing the system into two identical subvolumes. IT so, X-ray diffraction measurements would bt: expected ci to index ore a double-diamond (Pn3m) lattice with a unit cell length half that of the TEM lattice. Our measurements index on a Fd3m lattice with a similar repeat distance to thr TEM images. The PLB membrane is thus inherently asymmetric, probably as the result of the distribution of membrane protein. (C) 1998 Federation of European Biochemical Societies.
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| 55. |
- Bratt, T, et al.
(författare)
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Processing and secretion of rat alpha 1-microglobulin-bikunin expressed in eukaryotic cell lines
- 1994
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Ingår i: FEBS Letters. - : Wiley. - 0014-5793. ; 354:1, s. 57-61
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Tidskriftsartikel (refereegranskat)abstract
- The precursor protein alpha 1-microglobulin-bikunin was cleaved to the same degree whether expressed in CHO cells or in mutated CHO cells, RPE.40 cells, suggested to lack a functional form of the intracellular protease furin. Thus, alpha 1-microglobulin-bikunin probably is not cleaved in vivo by furin. However, simultaneous overexpression of the precursor and furin in COS, CHO and RPE.40 cells increased the cleavage, suggesting that compartmentalisation and concentrations of protease and precursor are important for the cleavage, besides the in vitro specificity. Expression of alpha 1-microglobulin and bikunin alone gave different protein patterns of SDS-PAGE as compared to expression of the precursor and subsequent cleavage, suggesting that the precursor protein is important for the post-translational handling of alpha 1-microglobulin and bikunin.
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| 56. |
- Gololobov, Mikhail Yu, et al.
(författare)
-
Organic solvent changes the chymotrypsin specificity with respect to nucleophiles
- 1992
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Ingår i: FEBS Letters. - 0014-5793. ; 307:3, s. 309-312
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Tidskriftsartikel (refereegranskat)abstract
- In α-chymotrypsin-catalyzed acyl-transfer reactions in water the specificity of the enzyme (the nucleophile reactivity of amino acid amides) is correlated with the substrate hydrophobicity and increases as the hydrophobicity of the side chain of the amino acid amides is increased. In a low water system (4% H2O) bulky amino acid amides are less efficient nucleophiles. The specificity of α-chymotrypsin towards the amino acid amides in acyl transfer reactions in this case does not depend on the hydrophobicity of the amino acid side chains but correlates with their size. Therefore, different factors can be responsible for the specificity of enzymes in water and in a mainly organic medium.
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| 57. |
- Möritz, Annemarie, et al.
(författare)
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Molecular cloning and sequence analysis of the cDNA encoding the human acrosin-trypsin inhibitor (HUSI-II)
- 1991
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Ingår i: FEBS Letters. - 0014-5793. ; 278:1, s. 127-130
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Tidskriftsartikel (refereegranskat)abstract
- A complete cDNA clone encoding the human acrosin-trypsin inhibitor HUSI-II has been isolated from a cDNA library of human testis and completely sequenced. The cDNA of 594 bp contained an open reading frame of 252 base pairs, The deduced amino acid sequence comprised the complete amino acid sequence of HUSI-II[1] and a putative signal peptide. Northern blotting analysis revealed that HUSI-II is synthesized in testis, epididymis and seminal vesicle, but not in the prostate gland.
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| 58. |
- Pierzchalski, P, et al.
(författare)
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Synthesis of alpha 1-microglobulin in cultured rat hepatocytes is stimulated by interleukin-6, leukemia inhibitory factor, dexamethasone and retinoic acid
- 1992
-
Ingår i: FEBS Letters. - 0014-5793. ; 298:2-3, s. 8-165
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Tidskriftsartikel (refereegranskat)abstract
- The secretion of alpha 1-microglobulin by primary cultures of rat hepatocytes was found to increase upon the addition of interleukin-6 or leukemia inhibitory factor, two mediators of acute phase response. This stimulatory effect was further enhanced by dexamethasone. alpha 1-Microglobulin is synthesized as a precursor also containing bikunin, and the precursor protein is cleaved shortly before secretion. Our results therefore suggest that both alpha 1-microglobulin and bikunin are acute phase reactants in rat hepatocytes. Furthermore, we found that retinoic acid, previously shown to be involved in the regulation of cell differentiation and development, also stimulated alpha 1-microglobulin synthesis. Only free, uncomplexed alpha 1-microglobulin (28,000 Da) was detected in the hepatocyte media, suggesting that the complex between alpha 1-microglobulin and alpha 1-inhibitor 3, found in rat serum, is formed outside the hepatocyte.
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| 59. |
- Rasmusson, Allan G., et al.
(författare)
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Component of the alternative oxidase localized to the matrix surface of the inner membrane of plant mitochondria
- 1990
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Ingår i: FEBS Letters. - 0014-5793. ; 259:2, s. 311-314
-
Tidskriftsartikel (refereegranskat)abstract
- In mitoplasts from Arum maculatum spadices, succinate dehydrogenase (EC 1.3.99.1) and the alternative, cyanide-resistant oxidase activity (measured as m-chlorobenzhydroxamic acid-sensitive duroquinol oxidation) was unaffected by treatment with trypsin. In contrast, when 85% inside-out submitochondrial particles were treated with trypsin the alternative oxidase activity was inhibited by about 50% and succinate dehydrogenase activity by about 40%. Thus, a trypsin-sensitive component of the alternative pathway is located on the inner surface of the inner mitochondrial membrane. After trypsin treatment of the inside-out submitochondrial particles the inhibited alternative oxidase activity was partly restored by including 0.7 M citrate in the assay medium. This indicates that trypsin does not destroy the active site but merely causes a conformational change in the enzyme, thereby lowering its activity.
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| 60. |
- Åkerström, B, et al.
(författare)
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Formation of the alpha 1-microglobulin chromophore in mammalian and insect cells : a novel post-translational mechanism?
- 1995
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Ingår i: FEBS Letters. - 0014-5793. ; 362:1, s. 4-50
-
Tidskriftsartikel (refereegranskat)abstract
- alpha 1-Microglobulin is an immunosuppressive plasma protein synthesized by the liver. The isolated protein is yellow-brown, but the hypothetical chromophore has not yet been identified. In this work, it is shown that a human liver cell line, HepG2, grown in a completely synthetic and serum-free medium, secretes alpha 1-microglobulin which is also yellow-brown, suggesting a de novo synthesis of the chromophore by the cells. alpha 1-Microglobulin isolated from the culture medium of insect cells transfected with the gene for rat alpha 1-microglobulin is also yellow-brown, suggesting that the gene carries information about the chromophore. Reduction and alkylation or removal of N- or O-linked carbohydrates by glycosidase treatment did not reduce the colour intensity of the protein. An internal dodecapeptide (amino acid positions 70-81 in human alpha 1-microglobulin) was also yellow-brown. The latter results indicate that the chromophore is linked to the polypeptide. In conclusion, the results suggest that the alpha 1-microglobulin gene carries information activating a post-translational protein modification mechanism which is present in mammalian and insect cells.
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| 61. |
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| 77. |
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| 78. |
- Hjertman, M, et al.
(författare)
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Evidence for protein dolichylation
- 1997
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Ingår i: FEBS letters. - : Wiley. - 0014-5793. ; 416:3, s. 235-238
-
Tidskriftsartikel (refereegranskat)
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