| 1. |
- Blomqvist, Maria K., 1975, et al.
(författare)
-
High-throughput analysis of sulfatides in cerebrospinal fluid using automated extraction and UPLC-MS/MS
- 2017
-
Ingår i: Journal of Lipid Research. - 0022-2275. ; 58:7, s. 1482-1489
-
Tidskriftsartikel (refereegranskat)abstract
- Sulfatides (STs) are a group of glycosphingolipids that are highly expressed in brain. Due to their importance for normal brain function and their potential involvement in neurological diseases, development of accurate and sensitive methods for their determination is needed. Here we describe a high-throughput oriented and quantitative method for the determination of STs in cerebrospinal fluid (CSF). The STs were extracted using a fully automated liquid/liquid extraction method and quantified using ultra-performance liquid chromatography coupled to tandem mass spectrometry. With the high sensitivity of the developed method, quantification of 20 ST species from only 100 mu l of CSF was performed. Validation of the method showed that the STs were extracted with high recovery (90%) and could be determined with low inter-and intra-day variation. Our method was applied to a patient cohort of subjects with an Alzheimer's disease biomarker profile. Although the total ST levels were unaltered compared with an age-matched control group, we show that the ratio of hydroxylated/nonhydroxylated STs was increased in the patient cohort. In conclusion, we believe that the fast, sensitive, and accurate method described in this study is a powerful new tool for the determination of STs in clinical as well as preclinical settings.-Blomqvist, M., J. Boren, H. Zetterberg, K. Blennow, J-E. Mansson, and M. Stahlman. High-throughput analysis of sulfatides in cerebrospinal fluid using automated extraction and UPLC-MS/MS.
|
|
| 2. |
- Hollestelle, Antoinette, et al.
(författare)
-
No clinical utility of KRAS variant rs61764370 for ovarian or breast cancer
- 2016
-
Ingår i: Gynecologic Oncology. - : Elsevier BV. - 0090-8258 .- 1095-6859. ; 141:2, s. 386-401
-
Tidskriftsartikel (refereegranskat)abstract
- Objective Clinical genetic testing is commercially available for rs61764370, an inherited variant residing in a KRAS 3′ UTR microRNA binding site, based on suggested associations with increased ovarian and breast cancer risk as well as with survival time. However, prior studies, emphasizing particular subgroups, were relatively small. Therefore, we comprehensively evaluated ovarian and breast cancer risks as well as clinical outcome associated with rs61764370. Methods Centralized genotyping and analysis were performed for 140,012 women enrolled in the Ovarian Cancer Association Consortium (15,357 ovarian cancer patients; 30,816 controls), the Breast Cancer Association Consortium (33,530 breast cancer patients; 37,640 controls), and the Consortium of Modifiers of BRCA1 and BRCA2 (14,765 BRCA1 and 7904 BRCA2 mutation carriers). Results We found no association with risk of ovarian cancer (OR = 0.99, 95% CI 0.94-1.04, p = 0.74) or breast cancer (OR = 0.98, 95% CI 0.94-1.01, p = 0.19) and results were consistent among mutation carriers (BRCA1, ovarian cancer HR = 1.09, 95% CI 0.97-1.23, p = 0.14, breast cancer HR = 1.04, 95% CI 0.97-1.12, p = 0.27; BRCA2, ovarian cancer HR = 0.89, 95% CI 0.71-1.13, p = 0.34, breast cancer HR = 1.06, 95% CI 0.94-1.19, p = 0.35). Null results were also obtained for associations with overall survival following ovarian cancer (HR = 0.94, 95% CI 0.83-1.07, p = 0.38), breast cancer (HR = 0.96, 95% CI 0.87-1.06, p = 0.38), and all other previously-reported associations. Conclusions rs61764370 is not associated with risk of ovarian or breast cancer nor with clinical outcome for patients with these cancers. Therefore, genotyping this variant has no clinical utility related to the prediction or management of these cancers.
|
|
| 3. |
- Novakova, Lenka, 1984, et al.
(författare)
-
Cerebrospinal fluid sulfatide isoforms lack diagnostic utility in separating progressive from relapsing-remitting multiple sclerosis.
- 2023
-
Ingår i: Multiple sclerosis and related disorders. - 2211-0356. ; 74
-
Tidskriftsartikel (refereegranskat)abstract
- Multiple sclerosis (MS) is an immune-mediated demyelinating disorder of the central nervous system. The glycosphingolipid sulfatide, a lipid particularly enriched in the myelin sheath, has been shown to be involved the maintenance of this specific membrane structure. Sulfatide in cerebrospinal fluid (CSF) may reflect demyelination, a dominating feature of MS. We investigated the diagnostic utility of CSF sulfatide isoform levels to separate different courses or phenotypes of MS disease.This was a mono-center, cross-sectional study of relapsing-remitting MS (RRMS) (n=45) and progressive MS (PMS) (n=42) patients (consisting of primary PMS (n=17) and secondary PMS (n=25)) and healthy controls (n=19). In total, 20 sulfatide isoforms were measured in CSF by liquid chromatography-mass spectrometry.CSF total sulfatide concentrations, as well as CSF sulfatide isoform distribution, did not differ across the study groups, and their levels were independent of disease course/phenotype, disease duration, time to conversion to secondary PMS, age, and disability in MS patients.CSF sulfatide isoforms lack diagnostic and prognostic utility as a biomarker for progressive MS.
|
|
| 4. |
- Novakova, Lenka, 1984, et al.
(författare)
-
Sulfatide isoform pattern in cerebrospinal fluid discriminates progressive MS from relapsing-remitting MS
- 2018
-
Ingår i: Journal of Neurochemistry. - : Wiley. - 0022-3042. ; 146:3, s. 322-332
-
Tidskriftsartikel (refereegranskat)abstract
- Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system (CNS). Several biomarkers including proteins and lipids have been reported in MS cerebrospinal fluid (CSF), reflecting different aspects of the pathophysiology particularly of relapsing-remitting MS (RRMS). Sulfatide, abundant in the myelin sheath and a proposed target for autoimmune attack in MS, has been reported altered in MS CSF. Here, we investigated the potential of CSF sulfatide and its isoforms as biomarkers in MS. A highly sensitive and quantitative mass spectrometry method was employed to determine levels of sulfatide isoforms in CSF from RRMS and progressive MS (PMS) patients, and healthy donors (HD). We demonstrate that levels of total CSF sulfatide and C24:1, C26:1, and C26:1-OH isoforms were significantly increased in PMS compared with RRMS patients and HD, while C23:0-OH was significantly decreased in CSF from PMS patients compared to the other two groups. Multivariate discriminant analysis showed that CSF sulfatide isoform pattern in PMS patients was distinct and non-overlapping with that of RRMS patients and HD. Sulfatide levels did not correlate with tested biomarkers or clinical parameters. The results suggest that CSF sulfatide isoform levels may be used to discriminate the phenotype ofMS and might play a role in the progression of the disease.
|
|
| 5. |
|
|
| 6. |
- Adler, Jan-Olof, et al.
(författare)
-
A broad range tagging spectrometer for the MAX-laboratory
- 1997
-
Ingår i: Nuclear Instruments & Methods in Physics Research. Section A: Accelerators, Spectrometers, Detectors, and Associated Equipment. - 0167-5087. ; 388:1-2, s. 17-26
-
Tidskriftsartikel (refereegranskat)abstract
- A broad range tagging spectrometer together with a new beam transport system for photonuclear experiments at the MAX-laboratory in Lund is described. The spectrometer consists of a quadrupole followed by an Elbek-type dipole and has a large momentum acceptance. It can produce both polarized and unpolarized tagged photons in the energy range 10–80 MeV with an energy resolution of about 300 keV.
|
|
| 7. |
- Anderberg, Mats, 1961-, et al.
(författare)
-
Strukturerade intervjuer inom missbruksvården : - som en grund för kunskapsutveckling
- 2009
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Anderberg, Mats & Dahlberg, Mikael (2009). Strukturerade intervjuer inom missbruksvården – som en grund för kunskapsutveckling (Structured interviews in substance abuse treatment - as a foundation for the development of knowledge). In the discussion on knowledge development in social work, structured interviews have attracted an increasing amount of attention. The aim of this thesis is to examine and analyze how structured interviews can serve as a basis in the compilation and development of knowledge for practice and research in addiction treatment. More precisely, it means a problematization of the methodological issues that are of central importance in studying treatment outcomes, in which structured interviews form the basis. The methodological aspects in focus here are scientific concepts such as reliability, validity and specific factors in the evaluation of treatment interventions. A further objective is to outline a model for analysis when compiling and evaluating treatment outcome. The five empirical studies have been conducted in their natural context, in organisations in which structured interviews with clients are carried out as an everyday routine and used for e.g. treatment planning. The structured interview DOK (a Swedish abbreviation for Documentation of clients) is used as a basis and an example for the empirical studies in this thesis. The validation studies show that the DOK interview generally attains a good level of reliability and validity, but also contains a small number of variables that do not live up to the requirements which may be imposed. Deficiencies in operationalization and question construction seem to have a large impact. Another conclusion is that it is possible to carry out validation studies, where each variable is examined separately and evaluated with the aid of both sound methodological starting points and relevant statistical theory. Another of the central questions, the thesis addresses, is how structured interviews can be used as a basis for evaluation of treatment. The two studies which highlight this issue show that it is obvious that structured interviews are suitable for such a purpose, through its standardized form and multi-dimensional nature. Validation of structured interviews or evaluation of social interventions should not be reduced to single numbers or values. The multidimensional nature of the structured interview shall also be reflected in the results and analysis. The thesis presents an analysis model for evaluating treatment outcome in relation to two empirical examples and foregoing theoretical starting points. Our view is that structured interviews can provide a link between practice and research and thus contribute to the development of knowledge in social work and substance abuse treatment.
|
|
| 8. |
|
|
| 9. |
- Andersson, S., et al.
(författare)
-
Sulfur recirculation for increased electricity production in Waste-to-Energy plants
- 2014
-
Ingår i: Waste Management. - : Elsevier BV. - 0956-053X .- 1879-2456. ; 34:1, s. 67-78
-
Tidskriftsartikel (refereegranskat)abstract
- Sulfur recirculation is a new technology for reducing boiler corrosion and dioxin formation. It was demonstrated in full-scale tests at a Waste to Energy plant in Goteborg (Sweden) during nearly two months of operation. Sulfur was recirculated as sulfuric acid from the flue gas cleaning back to the boiler, thus creating a sulfur loop. The new technology was evaluated by extensive measurement campaigns during operation under normal conditions (reference case) and operation with sulfur recirculation. The chlorine content of both fly ash and boiler ash decreased and the sulfur content increased during the sulfur recirculation tests. The deposit growth and the particle concentration decreased with sulfur recirculation and the dioxin concentration (I-TEQ) of the flue gas was reduced by approximately 25%. Sulfuric acid dew point measurements showed that the sulfuric acid dosage did not lead to elevated SO3 concentrations, which may otherwise induce low temperature corrosion. In the sulfur recirculation corrosion probe exposures, the corrosion rate decreased for all tested materials (16Mo3, Sanicro 28 and Inconel 625) and material temperatures (450 degrees C and 525 degrees C) compared to the reference exposure. The corrosion rates were reduced by 60-90%. Sulfur recirculation prevented the formation of transition metal chlorides at the metal/oxide interface, formation of chromate and reduced the presence of zinc in the corrosion products. Furthermore, measured corrosion rates at 525 degrees C with sulfur recirculation in operation were similar or lower compared to those measured at 450 degrees C material temperature in reference conditions, which corresponds to normal operation at normal steam temperatures. This implies that sulfur recirculation allows for higher steam data and electricity production without increasing corrosion.
|
|
| 10. |
|
|
