| 1. |
- Bowers, Robert M., et al.
(författare)
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Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea
- 2017
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Ingår i: Nature Biotechnology. - : NATURE PUBLISHING GROUP. - 1087-0156 .- 1546-1696. ; 35:8, s. 725-731
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Tidskriftsartikel (refereegranskat)abstract
- We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.
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| 2. |
- Drake, Marcus J, et al.
(författare)
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Structural and functional denervation of human detrusor after spinal cord injury.
- 2000
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Ingår i: Laboratory Investigation. - : Nature Publishing Group. - 0023-6837 .- 1530-0307. ; 80:10, s. 1491-9
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Tidskriftsartikel (refereegranskat)abstract
- The bladder receives an extensive nerve supply that is predominantly cholinergic, but several putative transmitters are present, some of which are colocalized. Previous studies have shown increased levels of sensory nerves, reduced inhibitory transmitters, and structural and functional changes in the excitatory input in unstable bladder conditions. The present study compared the end-organ nerve supply to the bladder in spinal cord injury (SCI) with uninjured controls. Acetylcholinesterase histochemistry and double-label immunofluorescence were used to investigate neurotransmitter content, with confocal laser scanning microscopy to assess colocalization. Organ bath studies provided functional correlates for the structural changes in the excitatory innervation. Control samples had dense innervation of the detrusor containing a diverse range of transmitters. Hyperreflexic SCI samples showed patchy denervation, and areflexic SCI samples were diffusely denervated. Vasoactive intestinal polypeptide-, neuropeptide Y-, neuronal nitric oxide synthase-, and galanin-immunoreactive nerve fibers were reduced from frequent or moderately frequent to infrequent or very infrequent in SCI. Calcitonin gene-related peptide-immunoreactive fibers were infrequent in controls and SCI samples. Patterns of colocalization were unchanged, but significantly fewer fibers expressed more than one transmitter. The subepithelial plexus was markedly reduced and several of the smaller coarse nerve trunks showed no immunoreactivity to the transmitters assessed. There was no reduction in sensitivity to electrical field stimulation of intrinsic nerves in SCI, but the maximum force generated by each milligram of bladder tissue and the peak force as a proportion of the maximum carbachol contraction were significantly reduced and the responses were protracted. There was no significant functional atropine-resistant neuromuscular transmission in controls or SCI. The reported findings have clinical implications in the management of chronic SCI and development of new treatments.
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| 3. |
- Gratzke, Christian, et al.
(författare)
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Cannabinor, a Selective Cannabinoid-2 Receptor Agonist, Improves Bladder Emptying in Rats With Partial Urethral Obstruction
- 2011
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Ingår i: JOURNAL OF UROLOGY. - : Elsevier Science B.V. Amsterdam. - 0022-5347 .- 1527-3792. ; 185:2, s. 731-736
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: We studied the effects of chronic treatment with the novel selective cannabinoid 2 receptor agonist cannabinor (Procter andamp; Gamble Pharmaceuticals, Cincinnatti, Ohio) on bladder function in conscious rats with partial urethral obstruction and on the functional properties of isolated detrusor muscle. Materials and Methods: A total of 24 female Sprague-Dawley(R) rats with surgically created partial urethral obstruction received daily intraperitoneal injections of 3 mg/kg cannabinor (12) or saline as controls (12) for 2 weeks. Cystometry was done, the rats were sacrificed and the bladders were prepared for in vitro studies. Results: Mean +/- SEM bladder weight was 0.97 +/- 0.15 gm in controls and 0.53 +/- 0.08 gm in cannabinor treated rats (p andlt; 0.05). There was no difference between the groups in the mean micturition interval, or mean baseline, threshold, flow or maximum pressure. In controls and cannabinor treated rats mean post-void residual volume was 0.28 +/- 0.07 and 0.06 +/- 0.02 ml, mean micturition compliance was 0.032 +/- 0.006 and 0.069 +/- 0.016 ml/cm H2O, and mean bladder wall force at the start of flow was 950 +/- 280 and 1,647 +/- 325 mN/gm, respectively (each p andlt; 0.05). Nonvoiding contractions were significantly less frequent in cannabinor treated rats than in controls. We noted no difference in carbachol (Sigma(R)) half maximum concentration between the groups but the carbachol maximum response in detrusor strips from cannabinor treated rats was significantly higher than that in control strips. Conclusions: In rats with partial urethral obstruction treated daily for 14 days with cannabinor bladder weight was lower, the ability to empty the bladder was preserved and nonvoiding contraction frequency was low compared to those in controls. Detrusor preparations from cannabinor treated rats showed a higher response to nerve stimulation than those from controls. Selective cannabinoid 2 receptor activation may be a novel principle to enable improved bladder function after partial urethral obstruction.
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| 4. |
- Hedlund, Jonas, et al.
(författare)
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Ultrathin oriented zeolite LTA films
- 1997
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Ingår i: Chemical Communications. - 1359-7345 .- 1364-548X. ; :13, s. 1193-1194
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Tidskriftsartikel (refereegranskat)abstract
- Ultrathin oriented films of zeolite LTA are prepared on single-crystal alumina supports by a method including adsorption of LTA seeds on the support followed by hydrothermal film crystallization.
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| 5. |
- Mintova, Svetlana, et al.
(författare)
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Continuous films of zeolite ZSM-5 on modified gold surfaces
- 1997
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Ingår i: Chemical Communications. - 1359-7345 .- 1364-548X. ; :1, s. 15-16
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Tidskriftsartikel (refereegranskat)abstract
- Thin continuous films of zeolite ZSM-5 on gold surfaces are prepared by a novel method including silanization, charge modification and seeding of the surface prior to the hydrothermal crystallization of the zeolite.
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| 6. |
- Mintova, Svetlana, et al.
(författare)
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Preparation of ZSM-5 films from template free precursors
- 1997
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Ingår i: Journal of Materials Chemistry. - 0959-9428 .- 1364-5501. ; 7:12, s. 2341-2342
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Tidskriftsartikel (refereegranskat)abstract
- Thin films of zeolite ZSM-5 on quartz substrates have been prepared in the absence of organic templates by growth of adsorbed seed crystals attached to a polymer-modified substrate surface.
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| 7. |
- Mintova, Svetlana, et al.
(författare)
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ZSM-5 films prepared from template free precursors
- 1998
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Ingår i: Journal of Materials Chemistry. - 0959-9428 .- 1364-5501. ; 8:10, s. 2217-2221
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Tidskriftsartikel (refereegranskat)abstract
- Thin continuous films of zeolite ZSM-5 were synthesized on quartz substrates. The substrates were first surface modified and covered by a monolayer of colloidal silicalite-1 seed crystals. These crystals were grown into continuous films with thicknesses in the range 230-3500 nm by hydrothermal treatment in a synthesis gel free from organic templates. The preferential orientation of the crystals constituting the film was initially one with thec-axis close to parallel to the substrate surface. During the course of crystallization this orientation changed to one with most of the crystals having the c-axes directed approximately 35° from perpendicular to the substrate surface. A mechanism explaining this behavior is proposed. The final thickness of the film was controlled by the synthesis time but also by the addition of seed crystals to the synthesis gel. Films prepared according to this method may be of great value for the development of zeolite based membranes.
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| 8. |
- Murray, Alison E., et al.
(författare)
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Roadmap for naming uncultivated Archaea and Bacteria
- 2020
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Ingår i: Nature Microbiology. - : NATURE PUBLISHING GROUP. - 2058-5276. ; 5:8, s. 987-994
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Tidskriftsartikel (refereegranskat)abstract
- The assembly of single-amplified genomes (SAGs) and metagenome-assembled genomes (MAGs) has led to a surge in genome-based discoveries of members affiliated with Archaea and Bacteria, bringing with it a need to develop guidelines for nomenclature of uncultivated microorganisms. The International Code of Nomenclature of Prokaryotes (ICNP) only recognizes cultures as 'type material', thereby preventing the naming of uncultivated organisms. In this Consensus Statement, we propose two potential paths to solve this nomenclatural conundrum. One option is the adoption of previously proposed modifications to the ICNP to recognize DNA sequences as acceptable type material; the other option creates a nomenclatural code for uncultivated Archaea and Bacteria that could eventually be merged with the ICNP in the future. Regardless of the path taken, we believe that action is needed now within the scientific community to develop consistent rules for nomenclature of uncultivated taxa in order to provide clarity and stability, and to effectively communicate microbial diversity. In this Consensus Statement, the authors discuss the issue of naming uncultivated prokaryotic microorganisms, which currently do not have a formal nomenclature system due to a lack of type material or cultured representatives, and propose two recommendations including the recognition of DNA sequences as type material.
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| 9. |
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| 10. |
- Omanakuttan, Giriprasanth, et al.
(författare)
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Surface emitting 1.5 mu m multi-quantum well LED on epitaxial lateral overgrowth InP/Si
- 2020
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Ingår i: Optical Materials Express. - : The Optical Society. - 2159-3930 .- 2159-3930. ; 10:7, s. 1714-1723
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Tidskriftsartikel (refereegranskat)abstract
- We demonstrate a surface emitting 1.5 mu m multi-quantum well (MQW) light-emitting diode (LED) on a 3-inch epitaxial lateral overgrowth (ELOG) InP/Si wafer. The enhanced crystalline quality of ELOG InP/Si is revealed by various characterization techniques, which gives rise to a MQW with high photoluminescence intensity at 1.5 mu m and interference fringes arising from the vertical Fabry-Perot cavity. The LED devices exhibited strong electroluminescence intensity that increased with pump current. Moreover, transparency current measurements indicate optical gain in the 1.5 mu m MQW on InP/Si. The results are encouraging for obtaining wafer scale 1.5 mu m surface emitting laser structures on silicon with further optimization.
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