| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Blanton, Michael R., et al.
(författare)
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Sloan Digital Sky Survey IV : Mapping the Milky Way, Nearby Galaxies, and the Distant Universe
- 2017
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Ingår i: Astronomical Journal. - : IOP Publishing Ltd. - 0004-6256 .- 1538-3881. ; 154:1
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Tidskriftsartikel (refereegranskat)abstract
- We describe the Sloan Digital Sky Survey IV (SDSS-IV), a project encompassing three major spectroscopic programs. The Apache Point Observatory Galactic Evolution Experiment 2 (APOGEE-2) is observing hundreds of thousands of Milky Way stars at high resolution and. high signal-to-noise ratios in the near-infrared. The Mapping Nearby Galaxies at Apache Point Observatory (MaNGA) survey is obtaining spatially resolved spectroscopy for thousands of nearby galaxies (median z similar to 0.03). The extended Baryon Oscillation Spectroscopic Survey (eBOSS) is mapping the galaxy, quasar, and neutral gas distributions between z similar to 0.6 and 3.5 to constrain cosmology using baryon acoustic oscillations, redshift space distortions, and the shape of the power spectrum. Within eBOSS, we are conducting two major subprograms: the SPectroscopic IDentification of eROSITA Sources (SPIDERS), investigating X-ray AGNs. and galaxies in X-ray clusters, and the Time Domain Spectroscopic Survey (TDSS), obtaining spectra of variable sources. All programs use the 2.5 m Sloan Foundation Telescope at the. Apache Point Observatory; observations there began in Summer 2014. APOGEE-2 also operates a second near-infrared spectrograph at the 2.5 m du Pont Telescope at Las Campanas Observatory, with observations beginning in early 2017. Observations at both facilities are scheduled to continue through 2020. In keeping with previous SDSS policy, SDSS-IV provides regularly scheduled public data releases; the first one, Data Release 13, was made available in 2016 July.
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| 3. |
- Cabrera, J., et al.
(författare)
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The planetary system around HD 190622 (TOI-1054): Measuring the gas content of low-mass planets orbiting F-stars
- 2023
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Ingår i: Astronomy and Astrophysics. - 0004-6361 .- 1432-0746. ; 675
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Tidskriftsartikel (refereegranskat)abstract
- Context. Giant planets are known to dominate the long-term stability of planetary systems due to their prevailing gravitational interactions, but they are also thought to play an important role in planet formation. Observational constraints improve our understanding of planetary formation processes such as the delivery of volatile-rich planetesimals from beyond the ice line into the inner planetary system. Additional constraints may come from studies of the atmosphere, but almost all such studies of the atmosphere investigate the detection of certain species, and abundances are not routinely quantitatively measured. Aims. Accurate measurements of planetary bulk parameters-that is, mass and density-provide constraints on the inner structure and chemical composition of transiting planets. This information provides insight into properties such as the amounts of volatile species, which in turn can be related to formation and evolution processes. Methods. The Transiting Exoplanet Survey Satellite (TESS) reported a planetary candidate around HD 190622 (TOI-1054), which was subsequently validated and found to merit further characterization with photometric and spectroscopic facilities. The KESPRINT collaboration used data from the High Accuracy Radial Velocity Planet Searcher (HARPS) to independently confirm the planetary candidate, securing its mass, and revealing the presence of an outer giant planet in the system. The CHEOPS consortium invested telescope time in the transiting target in order to reduce the uncertainty on the radius, improving the characterization of the planet. Results. We present the discovery and characterization of the planetary system around HD 190622 (TOI-1054). This system hosts one transiting planet, which is smaller than Neptune (3.087-0.053+0.058REarth, 7.7 ± 1.0 MEarth) but has a similar bulk density (1.43 ± 0.21 g cm-3) and an orbital period of 16 days; and a giant planet, not known to be transiting, with a minimum mass of 227.0 ± 6.7 MEarth in an orbit with a period of 315 days. Conclusions. Our measurements constrain the structure and composition of the transiting planet. HD 190622b has singular properties among the known population of transiting planets, which we discuss in detail. Among the sub-Neptune-sized planets known today, this planet stands out because of its large gas content.
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| 4. |
- Carter, Aarynn L., et al.
(författare)
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A benchmark JWST near-infrared spectrum for the exoplanet WASP-39 b
- 2024
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Ingår i: Nature Astronomy. - 2397-3366. ; In Press
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Tidskriftsartikel (refereegranskat)abstract
- A combined analysis of datasets across four JWST instrument modes provides a benchmark transmission spectrum for the Saturn-mass WASP-39 b. The broad wavelength range and high resolution constrain orbital and stellar parameters to below 1%.
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| 5. |
- Esparza-Borges, E., et al.
(författare)
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Detection of Carbon Monoxide in the Atmosphere of WASP-39b Applying Standard Cross-correlation Techniques to JWST NIRSpec G395H Data
- 2023
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Ingår i: Astrophysical Journal Letters. - 2041-8213 .- 2041-8205. ; 955:1
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Tidskriftsartikel (refereegranskat)abstract
- Carbon monoxide was recently reported in the atmosphere of the hot Jupiter WASP-39b using the NIRSpec PRISM transit observation of this planet, collected as part of the JWST Transiting Exoplanet Community Early Release Science Program. This detection, however, could not be confidently confirmed in the initial analysis of the higher-resolution observations with NIRSpec G395H disperser. Here we confirm the detection of CO in the atmosphere of WASP-39b using the NIRSpec G395H data and cross-correlation techniques. We do this by searching for the CO signal in the unbinned transmission spectrum of the planet between 4.6 and 5.0 μm, where the contribution of CO is expected to be higher than that of other anticipated molecules in the planet’s atmosphere. Our search results in a detection of CO with a cross-correlation function (CCF) significance of 6.6σ when using a template with only 12C16O lines. The CCF significance of the CO signal increases to 7.5σ when including in the template lines from additional CO isotopologues, with the largest contribution being from 13C16O. Our results highlight how cross-correlation techniques can be a powerful tool for unveiling the chemical composition of exoplanetary atmospheres from medium-resolution transmission spectra, including the detection of isotopologues.
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| 6. |
- France, Kevin, et al.
(författare)
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HST-COS Observations of Hydrogen, Helium, Carbon, and Nitrogen Emission from the SN 1987A Reverse Shock
- 2011
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Ingår i: Astrophysical Journal. - 0004-637X .- 1538-4357. ; 743:2, s. 186-
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Tidskriftsartikel (refereegranskat)abstract
- We present the most sensitive ultraviolet observations of Supernova 1987A to date. Imaging spectroscopy from the Hubble Space Telescope-Cosmic Origins Spectrograph shows many narrow (Δv ~ 300 km s-1) emission lines from the circumstellar ring, broad (Δv ~ 10-20 × 103 km s-1) emission lines from the reverse shock, and ultraviolet continuum emission. The high signal-to-noise ratio (>40 per resolution element) broad Lyα emission is excited by soft X-ray and EUV heating of mostly neutral gas in the circumstellar ring and outer supernova debris. The ultraviolet continuum at λ > 1350 Å can be explained by H I two-photon (2s 2 S 1/2-1s 2 S 1/2) emission from the same region. We confirm our earlier, tentative detection of N V λ1240 emission from the reverse shock and present the first detections of broad He II λ1640, C IV λ1550, and N IV] λ1486 emission lines from the reverse shock. The helium abundance in the high-velocity material is He/H = 0.14 ± 0.06. The N V/Hα line ratio requires partial ion-electron equilibration (Te /Tp ≈ 0.14-0.35). We find that the N/C abundance ratio in the gas crossing the reverse shock is significantly higher than that in the circumstellar ring, a result that may be attributed to chemical stratification in the outer envelope of the supernova progenitor. The N/C abundance may have been stratified prior to the ring expulsion, or this result may indicate continued CNO processing in the progenitor subsequent to the expulsion of the circumstellar ring. Based on observations made with the NASA/ESA Hubble Space Telescope, obtained from the data archive at the Space Telescope Science Institute. STScI is operated by the Association of Universities for Research in Astronomy, Inc., under NASA contract NAS 5-26555.
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| 7. |
- France, Kevin, et al.
(författare)
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MAPPING HIGH-VELOCITY H alpha AND Ly alpha EMISSION FROM SUPERNOVA 1987A
- 2015
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Ingår i: Astrophysical Journal Letters. - 2041-8205 .- 2041-8213. ; 801:1
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Tidskriftsartikel (refereegranskat)abstract
- We present new Hubble Space Telescope images of high-velocity H alpha and Ly alpha emission in the outer debris of SN 1987 A. The Ha images are dominated by emission from hydrogen atoms crossing the reverse shock (RS). For the first time we observe emission from the RS surface well above and below the equatorial. ring (ER), suggesting a bipolar or conical structure perpendicular to the ring plane. Using the H alpha imaging, we measure the mass flux of hydrogen atoms crossing the RS front, in the velocity intervals (-7500 < V-obs < -2800 km s(-1)) and (1000 < V-obs < 7500 km s(-1)), (M)(H) over dot = 1.2 x 10(-3) M-circle dot yr(-1). We also present the first Ly alpha imaging of the whole remnant and new Chandra X-ray observations. Comparing the spatial distribution of the Ly alpha and X-ray emission, we observe that the majority of the high-velocity Ly alpha emission originates interior to the ER. The observed Ly alpha/H alpha photon ratio, < R(L alpha/H alpha)> approximate to 17, is significantly higher than the theoretically predicted ratio of approximate to 5 for neutral atoms crossing the RS front. We attribute this excess to Ly alpha emission produced by X-ray heating of the outer debris. The spatial orientation of the Ly alpha and X-ray emission suggests that X-ray heating of the outer debris is the dominant Ly alpha production mechanism in SN 1987 A at this phase in its evolution.
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| 8. |
- France, Kevin, et al.
(författare)
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MAPPING high-velocity Hα and Lyα emission from supernova 1987A
- 2015
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Ingår i: Astrophysical Journal Letters. - 2041-8205 .- 2041-8213. ; 801:1
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Tidskriftsartikel (refereegranskat)abstract
- We present new Hubble Space Telescope images of high-velocity H alpha and Ly alpha emission in the outer debris of SN 1987 A. The Ha images are dominated by emission from hydrogen atoms crossing the reverse shock (RS). For the first time we observe emission from the RS surface well above and below the equatorial. ring (ER), suggesting a bipolar or conical structure perpendicular to the ring plane. Using the H alpha imaging, we measure the mass flux of hydrogen atoms crossing the RS front, in the velocity intervals (-7500 < V-obs < -2800 km s(-1)) and (1000 < V-obs < 7500 km s(-1)), (M)(H) over dot = 1.2 x 10(-3) M-circle dot yr(-1). We also present the first Ly alpha imaging of the whole remnant and new Chandra X-ray observations. Comparing the spatial distribution of the Ly alpha and X-ray emission, we observe that the majority of the high-velocity Ly alpha emission originates interior to the ER. The observed Ly alpha/H alpha photon ratio, < R(L alpha/H alpha)> approximate to 17, is significantly higher than the theoretically predicted ratio of approximate to 5 for neutral atoms crossing the RS front. We attribute this excess to Ly alpha emission produced by X-ray heating of the outer debris. The spatial orientation of the Ly alpha and X-ray emission suggests that X-ray heating of the outer debris is the dominant Ly alpha production mechanism in SN 1987 A at this phase in its evolution.
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| 9. |
- France, Kevin, et al.
(författare)
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Observing Supernova 1987A with the Refurbished Hubble Space Telescope
- 2010
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 329:5999, s. 1624-1627
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Tidskriftsartikel (refereegranskat)abstract
- Observations with the Hubble Space Telescope (HST), conducted since 1990, now offer an unprecedented glimpse into fast astrophysical shocks in the young remnant of supernova 1987A. Comparing observations taken in 2010 with the use of the refurbished instruments on HST with data taken in 2004, just before the Space Telescope Imaging Spectrograph failed, we find that the Ly alpha and H alpha lines from shock emission continue to brighten, whereas their maximum velocities continue to decrease. We observe broad, blueshifted Ly alpha, which we attribute to resonant scattering of photons emitted from hot spots on the equatorial ring. We also detect N v lambda lambda 1239, 1243 angstrom line emission, but only to the red of Ly alpha. The profiles of the N v lines differ markedly from that of H alpha, suggesting that the N4+ ions are scattered and accelerated by turbulent electromagnetic fields that isotropize the ions in the collisionless shock.
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| 10. |
- Fransson, Claes, et al.
(författare)
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THE DESTRUCTION OF THE CIRCUMSTELLAR RING OF SN 1987A
- 2015
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Ingår i: Astrophysical Journal Letters. - 2041-8205 .- 2041-8213. ; 806:1
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Tidskriftsartikel (refereegranskat)abstract
- We present imaging and spectroscopic observations with Hubble Space Telescope and Very Large Telescope of the ring of SN 1987A from 1994 to 2014. After an almost exponential increase of the shocked emission from the hotspots up to day similar to 8000 (similar to 2009), both this and the unshocked emission are now fading. From the radial positions of the hotspots we see an acceleration of these up to 500-1000 km s(-1), consistent with the highest spectroscopic shock velocities from the radiative shocks. In the most recent observations (2013 and 2014), we find several new hotspots outside the inner ring, excited by either X-rays from the shocks or by direct shock interaction. All of these observations indicate that the interaction with the supernova ejecta is now gradually dissolving the hotspots. We predict, based on the observed decay, that the inner ring will be destroyed by similar to 2025.
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